A Comparison of the Cystotome-Assisted Prechop and Phaco-Chop Phacoemulsification Nucleotomy Techniques.

BACKGROUND The aim of this study was to compare the intra-operative parameters and post-operative outcomes of cataract surgery performed using the cystotome-assisted prechop (CAP) and phaco-chop techniques. MATERIAL AND METHODS Fifty-two eyes with age-related cataract in the CAP group, and 63 eyes in the phaco-chop group were enrolled for analysis in this study, and the surgical outcomes were reported 1 day and/or 1 week, and 1 month post-operatively. RESULTS The CAP technique was associated with statistically significantly lower cumulative dissipated energy compared with the phaco-chop technique (P<0.001). The mean endothelial cell loss in the CAP group was statistically significantly lower than that of the phaco-chop group 1 week (5.6±5.9% versus 8.8±8.7%, P=0.020) and 1 month post-operatively (6.3±6.8% versus 9.8±9.9%, P=0.026). The change in the central corneal thickness between the 2 groups was significantly different at 1 day post-operatively (3.3±3.1% versus 4.9±4.6%, P=0.036). The change in the 8.0 mm central corneal volume between the 2 groups was significantly different at 1 day and 1 week post-operatively (6.5±6.1% versus 10.9±7.9%, P=0.001; 3.2±4.7% versus 5.4±5.7%, P=0.029, respectively). CONCLUSIONS The CAP technique showed lower ultrasound energy consumption and less endothelial damage and corneal edema than the phaco-chop technique. It might therefore prove a cost-effective prechop method for cataract surgery.

[The changes of histology and biochemical parameters in retina of the patient with diabetic retinopathy].

OBJECTIVE: To observe and estimate the distribution the changes of histology and biochemical parameters in retina of the patient with diabetic retinopathy. METHODS: Retinas were divided into quadrants and the lesions of diabetic retinopathy were compared among quadrants. the vasculature was isolated by trypsin-digest, homogenates taken from temporal and nasal quadrant were used to explore regional differences in the expression of Glut-1, PKCbeta and activity of caspase1. RESULTS: Microaneurysms, acellular capillaries and pericytes ghosts were significantly increased in the temporal retina than in the nasal retina. Activity of the pro-inflammatory protease, caspase1, was the only biochemical abnormality which was found significantly difference between quadrants. Glut-1 expression was decreased in diabetes, but there was no significant difference between quadrants. Expression of PKCbeta in both temporal and nasal retina was tended to be greater in diabetic than in non diabetic patients. CONCLUSION: Retinal microvascular disease does not develop uniformly across the retina, even the different regions are exposed to the same level of hyperglycemia. Regional differences play an important role in the response of the retinal microvasculature to hyperglycemia.