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1.
Opt Express ; 28(12): 17898-17899, 2020 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-32679991

RESUMO

We have found an error in our work reported in [Opt. Express26(3), 3557 (2018)], which we correct in this erratum. We used incorrect data for the experimentally measured values of power of the fibre trap and power of the conventional optical tweezers (OT) used to 'break' the fibre trap. Using the correct data, Ffibre and Q (force and quality) of the multicore fibre tweezer are re-calculated. In this erratum, we communicate the correct values of Ffibre and Q and publish a revised Fig. 7 that contains results based on the correct data. Based on the revised result, two statements, in the abstract and conclusions, are also revised. The fabrication method, technique and general conclusion remain unaffected.

2.
Opt Express ; 26(3): 3557-3567, 2018 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-29401883

RESUMO

Optical tweezing is a non-invasive technique that can enable a variety of single cell experiments; however, it tends to be based on a high numerical aperture (NA) microscope objective to both deliver the tweezing laser light and image the sample. This introduces restrictions in system flexibility when both trapping and imaging. Here, we demonstrate a novel, high NA tweezing system based on micro-machined multicore optical fibers. Using the machined, multicore fiber tweezer, cells are optically manipulated under a variety of microscopes, without requiring a high NA objective lens. The maximum NA of the fiber-based tweezer demonstrated is 1.039. A stable trap with a maximum total power 30 mW has been characterized to exert a maximum optical force of 26.4 pN, on a trapped, 7 µm diameter yeast cell. Single cells are held 15-35 µm from the fiber end and can be manipulated in the x, y and z directions throughout the sample. In this way, single cells are controllably trapped under a Raman microscope to categorize the yeast cells as live or dead, demonstrating trapping by the machined multicore fiber-based tweezer decoupled from the imaging or excitation objective lens.


Assuntos
Fibras Ópticas , Pinças Ópticas , Manejo de Espécimes/métodos , Leveduras/citologia , Imageamento Tridimensional/métodos
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