qSR: a quantitative super-resolution analysis tool reveals the cell-cycle dependent organization of RNA Polymerase I in live human cells.

We present qSR, an analytical tool for the quantitative analysis of single molecule based super-resolution data. The software is created as an open-source platform integrating multiple algorithms for rigorous spatial and temporal characterizations of protein clusters in super-resolution data of living cells. First, we illustrate qSR using a sample live cell data of RNA Polymerase II (Pol II) as an example of highly dynamic sub-diffractive clusters. Then we utilize qSR to investigate the organization and dynamics of endogenous RNA Polymerase I (Pol I) in live human cells, throughout the cell cycle. Our analysis reveals a previously uncharacterized transient clustering of Pol I. Both stable and transient populations of Pol I clusters co-exist in individual living cells, and their relative fraction vary during cell cycle, in a manner correlating with global gene expression. Thus, qSR serves to facilitate the study of protein organization and dynamics with very high spatial and temporal resolutions directly in live cell.

Women and the global tobacco epidemic: nurses call to action.

BACKGROUND: Tobacco consumption is increasing among women across the globe at alarming rates. Without effective intervention, the smoking prevalence among women will nearly triple over the next generation. These trends are potentially more threatening when considering how tobacco intersects women's lives, regardless if they use tobacco products or not. AIM: A review and analysis of the literature is conducted to examine the scope of tobacco's global effect on the multiple dimensions of women's health. METHOD: Medline (1990-2003), Cumulative Index to Nursing and Allied Health Literature (1990-2003) and World Health Organization databases were searched for related topics. Keywords for searches included global health, tobacco, women and nursing. FINDINGS: The epidemiology and prevalence of tobacco use among women are presented and its impact on women globally. Using an ecological perspective, the consequences of tobacco are analysed within the contexts of health, social, environment, economic and policy as it relates to women, their families and their communities. IMPLICATIONS: Nurses are in prime positions to empower individuals, families, communities and nations in the prevention and treatment of tobacco use. Health for all women continues to be a call for equity and social justice. Recommendations are provided for nursing practice, education, theory, research and policy to address this global health concern.

Provider feedback improves adherence with AHCPR Smoking Cessation Guideline.

BACKGROUND: This study evaluated the effect of primary care providers' adherence with the AHCPR Smoking Cessation Guideline after receiving a multicomponent intervention. METHODS: A quasi-experimental study with one intervention and one control team was conducted in a southeastern Veterans Affairs Medical Center primary care setting. During phase I, chart reviews were conducted to measure baseline provider adherence and documentation of the four A's (ask, advise, assist, arrange). In phase II, the intervention team received a single educational session on the AHCPR Guideline, four A's, and tobacco dependence treatment. This was followed by chart reviews of patients seen 4 to 8 weeks after the educational intervention to measure provider adherence and documentation of the four A's. During phase III, the intervention team received individual and team feedback from the chart reviews in phases I and II and booster education on the AHCPR Guideline. Chart reviews were conducted from patient visits 4 to 8 weeks after the feedback and booster education to determine provider adherence and documentation of the four A's. RESULTS: A nested repeated measures two-factor analysis of variance was performed for each of the following outcomes: ask, advise, assist, and arrange. Data analyses revealed that both the control and the intervention teams had 100% compliance in asking the patient about smoking status. There was a prestudy implementation of the vital sign stamp that included smoking status in this setting. Education on tobacco dependence and the AHCPR Guideline had no significant impact on provider performance with the advisement, assistance, and arrangement of follow-up. However, significant improvements occurred in the intervention team in the advisement (P = 0.05), assistance (P = 0.001), and arrangement of follow-up (P = 0.001) phase after individual and team feedback was provided. This research supports the fact that feedback impacts individuals and team performances and facilitated positive system changes to improve provider adherence with the AHCPR recommendations in treating tobacco dependence.

Heart disease mortality in women: racial, ethnic, and geographic disparities.

Heart disease is the leading cause of death in women. The recent Centers for Disease Control and Prevention (CDC) landmark document, Women and Heart Disease: An Atlas of Racial and Ethnic Disparities in Mortality, identifies the relationship between geography, race, ethnicity, gender, and the determinants of mortality. Overwhelming evidence supports that African American women, especially those residing in the South, experience higher heart disease death rates than women of other ethnic, racial, and geographic origins. This publication presents a unique opportunity for health care providers to use derived county-specific data in education, research, and provision of health promotion and disease prevention services to all women.

Race, ethnicity, and geography: disparities in heart disease in women of color.

Cardiovascular disease (CVD) is the leading cause of death in women. Misconceptions about the importance of CVD still persist. These myths affect awareness of CVD and the urgency with which women present for treatment after symptoms develop. Modifiable and nonmodifiable risk factors are more prevalent among ethnic minority women in concentrated geographical locations. The recent publication of the CDC women's atlas presents a unique opportunity for healthcare providers to use derived county-specific data in education, research, and delivery of health promotion and disease prevention services to women of color. Specific recommendations for the nursing profession are provided to assist in eliminating CVD disparities for all women.

A model nurse practitioner-managed smoking cessation clinic.

PURPOSE/OBJECTIVES: To describe an intensive nurse practitioner (NP)-managed smoking cessation clinic that evolved from a primary-care quality-management initiative. DATA SOURCE: Published articles, abstracts, books, clinical experience, and clinical data. DATA SYNTHESIS: Smoking continues to be the leading cause of preventable deaths in the United States and accounts for 87% of all lung cancers. Although smoking is responsible for nearly 30% of all cancer deaths, smoking prevalence rates remain stagnant in adults and are continuing to increase in adolescents. Twenty-five percent of all Americans smoke. An NP-managed clinic was developed within a large teaching hospital located in the southeastern United States based on the Agency for Health Care Policy and Research guideline for smoking cessation. The clinic provided effective smoking cessation interventions that can be replicated by experienced nurses with a smoking cessation background. All nurses have opportunities to assist patients to stop smoking through brief counseling and minimum interventions. CONCLUSIONS: Nurses can effectively manage an intensive smoking cessation clinic that is utilized by the interdisciplinary team to treat referrals. Cessation rates were high because therapies combined intensive behavioral sessions and pharmacologic approaches rather than either single modality. IMPLICATIONS FOR NURSING PRACTICE: Nurses can replicate this practice in a variety of healthcare settings. Innovations in clinical practice often facilitate research studies to further define effective approaches for smoking cessation. Nurses need to identify and target smoking as the serious health problem it is and conduct much-needed research on cessation approaches within the inpatient and outpatient settings.

Implementing the Agency for Health Care Policy and Research smoking cessation guidelines: a call to action.

Smoking is the single most preventable cause of death in our society. Each year, cigarettes kill more Americans than AIDS, alcohol, car accidents, fires, illegal drugs, murders, and suicides combined. An estimated 46 million adults in the United States smoke--28% of all men and 23.5% of all women. Seventy percent of Americans who smoke say that they would like to quit. Additionally, 70% who smoke visit a healthcare provider each year. Unfortunately, half of these who seek health care each year say they have never been advised to quit smoking or provided specific strategies to be successful at quitting. In 1996, the Agency for Health Care Policy and Research (AHCPR) published smoking cessation materials in several formats that target different audiences. This article describes the significant health problems of smoking and the AHCPR publications for smoking cessation. Providers are encouraged to identify smoking cessation as a priority and incorporate the AHCPR guidelines into routine health care for all patient encounters.

A QI project to reduce nosocomial blood loss.

Nosocomial blood loss in the intensive care setting can be significant and even lead to anemia. The Quality Improvement (QI) Project described in this article resulted in standards to decrease the volume of blood loss related to laboratory testing in critically ill patients.