Ethylene Is Crucial in Abscisic Acid-Mediated Modulation of Seed Vigor, Growth, and Photosynthesis of Salt-Treated Mustard.

The current study explored the differential interaction between ethylene (ET) and abscisic acid (ABA) in relation to salt stress in mustard (Brassica juncea L.) plants. Significant reductions in seed germination, growth, and photosynthesis were observed with 100 mmol NaCl. Among the cultivars tested, the Pusa Vijay cultivar was noted as ET-sensitive. Pusa Vijay responded maximally to an application of 2.0 mmol ethephon (Eth; 2-chloethyl phosphonic acid-ethylene source), and exhibited the greatest growth, photosynthesis, activity of 1-aminocyclopropane carboxylic acid (ACC) synthase (ACS), and ET evolution. Notably, Eth (2.0 mmol) more significantly improved the seed germination percentage, germination and vigor index, amylase activity, and reduced H2O2 content under salt stress, while ABA (25 µmol) had negative effects. Moreover, the individual application of Eth and ABA on Pusa Vijay under both optimal and salt-stressed conditions increased the growth and photosynthetic attributes, nitrogen (N) and sulfur (S) assimilation, and antioxidant defense machinery. The addition of aminoethoxyvinylglycine (0.01 µmol AVG, ET biosynthesis inhibitor) to ABA + NaCl-treated plants further added to the effects of ABA on parameters related to seed germination and resulted in less effectiveness of growth and photosynthesis. In contrast, the effects of Eth were seen with the addition of fluoridone (25 µmol Flu, ABA biosynthesis inhibitor) to Eth + NaCl. Thus, it can be suggested that ET is crucial for alleviating salt-induced inhibition in seed germination, growth, and photosynthesis, while ABA collaborated with ET to offer protection by regulating nutrient assimilation and enhancing antioxidant metabolism. These findings provide insight into the complex regulatory processes involved in ET-ABA interaction, enhancing our understanding of plant growth and development and the mitigation of salt stress in mustard. It opens pathways for developing hormonal-based strategies to improve crop productivity and resilience, ultimately benefiting agricultural practices amidst a challenging environment.

Phytoremediation of Potentially Toxic Elements: Role, Status and Concerns.

Environmental contamination with a myriad of potentially toxic elements (PTEs) is triggered by various natural and anthropogenic activities. However, the industrial revolution has increased the intensity of these hazardous elements and their concentration in the environment, which, in turn, could provoke potential ecological risks. Additionally, most PTEs pose a considerable nuisance to human beings and affect soil, aquatic organisms, and even nematodes and microbes. This comprehensive review aims to: (i) introduce potentially toxic elements; (ii) overview the major sources of PTEs in the major environmental compartments; (iii) briefly highlight the major impacts of PTEs on humans, plants, aquatic life, and the health of soil; (iv) appraise the major methods for tackling PTE-caused pollution; (v) discuss the concept and applications of the major eco-technological/green approaches (comprising phytoextraction, rhizofiltration, phytostabilization, phytovolatilization, and phytorestoration); (vi) highlight the role of microbes in phytoremediation under PTE stress; and (vii) enlighten the major role of genetic engineering in advancing the phytoremediation of varied PTEs. Overall, appropriate strategies must be developed in order to stop gene flow into wild species, and biosafety issues must be properly addressed. Additionally, consistent efforts should be undertaken to tackle the major issues (e.g., risk estimation, understanding, acceptance and feasibility) in order to guarantee the successful implementation of phytoremediation programs, raise awareness of this green technology among laymen, and to strengthen networking among scientists, stakeholders, industrialists, governments and non-government organizations.

CD-MAWS: An Alignment-Free Phylogeny Estimation Method Using Cosine Distance on Minimal Absent Word Sets.

Multiple sequence alignment has been the traditional and well established approach of sequence analysis and comparison, though it is time and memory consuming. As the scale of sequencing data is increasing day by day, the importance of faster yet accurate alignment-free methods is on the rise. Several alignment-free sequence analysis methods have been established in the literature in recent years, which extract numerical features from genomic data to analyze sequences and also to estimate phylogenetic relationship among genes and species. Minimal Absent Word (MAW) is an effective concept for representing characteristics of a sequence in an alignment-free manner. In this study, we present CD-MAWS, a distance measure based on cosine of the angle between composition vectors constructed using minimal absent words, for sequence analysis in a computationally inexpensive manner. We have benchmarked CD-MAWS using several AFProject datasets, such as Fish mtDNA, E.coli, Plants, Shigella and Yersinia datasets, and found it to perform quite well. Applied on several other biological datasets such as mammal mtDNA, bacterial genomes and viral genomes, CD-MAWS resolved phylogenetic relationships similar to or better than state-of-the-art alignment-free methods such as Mash, Skmer, Co-phylog and kSNP3.

Nitric Oxide and Salicylic Acid Regulate Glutathione and Ethylene Production to Enhance Heat Stress Acclimation in Wheat Involving Sulfur Assimilation.

Phytohormones have a role in stress adaptation. The major mechanism underlying the role of exogenously-sourced nitric oxide (NO; as sodium nitroprusside, SNP: 50.0 µM) and salicylic acid (SA; 0.5 mM) in the presence of 2.0 mM SO4-2 was assessed in heat stress (HS; 40 °C for 6 h daily for 15 days) tolerance in wheat (Triticum aestivum L. cv. HD-3226). The cultivar HD-3226 possessed high photosynthetic sulfur use efficiency (p-SUE) among the six cultivars screened. Plants grown under HS exhibited an increased content of reactive oxygen species (ROS; including superoxide radical and hydrogen peroxide) and extent of lipid peroxidation with a consequent reduction in photosynthesis and growth. However, both NO and SA were found to be protective against HS via enhanced S assimilation. Their application reduced oxidative stress and increased the activity of antioxidant enzymes. NO or SA supplementation along with S under HS recovered the losses and improved photosynthesis and growth. The use of SA inhibitor (2-aminoindane-2-phosphonic acid; AIP) and NO scavenger (cPTIO) confirmed that the mitigating effects of SA and NO involved induction in S assimilation.

Phylogenetic Analyses of SARS-CoV-2 Strains Reveal Its Link to the Spread of COVID-19 Across the Globe.

This study leveraged the phylogenetic analysis of more than 10K strains of novel coronavirus (SARS-CoV-2) from 67 countries. Due to the requirement of high-end computational power for phylogenetic analysis, we leverage a fast yet highly accurate alignment-free method to develop the phylogenetic tree out of all the strains of novel coronavirus. K-Means clustering and PCA-based dimension reduction technique were used to identify a representative strain from each location. The resulting phylogenetic tree was able to highlight evolutionary relationships of SARS-CoV-2 genome and, subsequently, linked to the interpretation of facts and figures across the globe for the spread of COVID-19. Our analysis revealed that the geographical boundaries could not be explained by the phylogenetic analysis of novel coronavirus as it placed different countries from Asia, Europe and the USA in very close proximity in the tree. Instead, the commute of people from one country to another is the key to the spread of COVID-19. We believe our study will support the policymakers to contain the spread of COVID-19 globally.

Arbuscular mycorrhizae: natural modulators of plant-nutrient relation and growth in stressful environments.

The human population is increasing by 0.96% annually and is estimated to reach from 7.3 to 9 billion in 2050 and 11 billion in 2100. The world's agriculture is under pressure to produce more food and ensure food security. On the other hand, around 40% of the cultivable land is already degraded due to various factors including urbanization, soil sealing, soil acidification, salinization, soil erosion, and contamination. Arbuscular mycorrhizal fungi (AMF) constitute a unique group of root obligate symbiont that exchange mutual benefits with about 90% of terrestrial plants and represents a key link between plants and soil mineral nutrients. Literature is scanty on the studies on massive inoculation of AMF in food crops in agronomic settings, and thereby achieving efficient uptake and minimization of the major soil nutrients, eventually meeting our food demand under increasing and inevitable stressed environments. Given above, this review aimed to (i) introduce agricultural soil-contamination, and the relation of soil microbiome with the health of soils and plants; (ii) briefly overview AMF; (iii) highlight AMF role as a bioinoculant, and enhancer of efficient uptake and loss-minimization of nutrients; (iv) appraise literature available on AMF role in the regulation of growth and nutrition mainly in vegetable, horticultural crops and fruit trees; (v) enlighten the role and major mechanisms underlying AMF-mediated regulation of plant growth and nutrition under major biotic and abiotic stresses; (vi) highlight AMF role in the minimization of greenhouse gas emissions; and (vii) list major aspects so far unexplored in the current context.

Ethylene-nitrogen synergism induces tolerance to copper stress by modulating antioxidant system and nitrogen metabolism and improves photosynthetic capacity in mustard.

This study aimed to test the efficiency of ethylene (Eth; 200 µL L-1 ethephon) in presence or absence of nitrogen (N; 80 mg N kg-1 soil) in protecting photosynthetic apparatus from copper (Cu; 100 mg Cu kg-1 soil) stress in mustard (Brassica juncea L.) and to elucidate the physio-biochemical modulation for Eth plus N-induced Cu tolerance. Elevated Cu-accrued reductions in photosynthesis and growth were accompanied by significantly higher Cu accumulation in leaves and oxidative stress with reduced assimilation of N and sulfur (S). Ethylene in coordination with N considerably reduced Cu accumulation, lowered lipid peroxidation, lignin accumulation, and contents of reactive oxygen species (hydrogen peroxide, H2O2, and superoxide anion, O2•-), and mitigated the negative effect of Cu on N and S assimilation, accumulation of non-protein thiols and phytochelatins, enzymatic, and non-enzymatic antioxidants (activity of ascorbate peroxidase, APX, and glutathione reductase, GR; content of reduced glutathione, GSH, and ascorbate, AsA), cell viability, photosynthesis, and growth. Overall, the effect of ethylene-nitrogen synergism was evident on prominently mitigating Cu stress and protecting photosynthesis. The approach of supplementing ethylene with N may be used as a potential tool to restrain Cu stress, and protect photosynthesis and growth of mustard plants.

Control of Elevated Ion Accumulation, Oxidative Stress, and Lipid Peroxidation with Salicylic Acid-Induced Accumulation of Glycine Betaine in Salinity-Exposed Vigna radiata L.

The identification of the sustainable approaches is required for the minimization of adverse impact of worldwide increasing soil salinity on plant growth, development, and productivity. This study investigated the protective role and major mechanism underlying salicylic acid (SA; 0.1, 0.5, or 1.0 mM)-induced glycine betaine (GB)-mediated tolerance to salinity (50 mM NaCl) in mungbean (Vigna radiata L. cultivar Punt Mung). The supply of 0.5 mM SA maximally increased the accumulation of GB (>40%) with respect to the control. This was further corroborated with the increase in water potential, antioxidant system (reduced glutathione (GSH), GSH/GSSG redox state, and glutathione reductase (GR) activity) and decreased Na+ and Cl- accumulation, Na+/K+ ratio, oxidative stress, and lipid peroxidation. This was also associated with the increased photosynthesis (14-18%) and growth (7-12%) parameters. Overall, SA-induced accumulation of GB protected photosynthesis and growth against 50 mM NaCl-accrued impacts in V. radiata through minimizing the accumulation of Na+ and Cl- ions, oxidative stress, and maintaining high GSH level that led to reduced cellular redox environment.

Hydrogen peroxide potentiates defense system in presence of sulfur to protect chloroplast damage and photosynthesis of wheat under drought stress.

The involvement of hydrogen peroxide (H2 O2 ) combined with sulfur (S) was studied in the protection of the photosynthetic performance of wheat (Triticum aestivum L.) under drought stress. The mechanisms of S-assimilation, the activity of antioxidants, glucose sensitivity, water and osmotic relations and abscisic acid (ABA) content were the focus. The combined application of 50 µM H2 O2 and 100 mg S kg-1 soil (sulfur) resulted in a marked increase in S-assimilation and activity of antioxidant enzymes, with decreased glucose sensitivity and ABA content causing improvement in the structure and function of the photosynthetic apparatus under drought stress. The photosynthetic performance, pigment system (PS) II activity, and growth were improved conspicuously by H2 O2 in the presence of S, as H2 O2 induced S-assimilation capacity, the activity of antioxidant enzymes, and GSH synthesis under drought stress. Our study shows that H2 O2 is more effective in the reversal of drought stress in the presence of S through its influence on S-assimilation, glucose sensitivity, and antioxidant system. These results provide evidence for the effectiveness of H2 O2 in improving photosynthesis under drought stress in the presence of S.

The outcomes of the functional interplay of nitric oxide and hydrogen sulfide in metal stress tolerance in plants.

Phytotoxicity of metals constraints plants health, metabolism and productivity. The sustainable approaches for minimizing major metals-accrued phytotoxicity have been least explored. The gasotransmitters signaling molecules such as nitric oxide (NO) and hydrogen sulfide (H2S) play a significant role in the mitigation of major consequences of metals stress. Versatile gaseous signaling molecules, NO and H2S are involved in the regulation of various physiological processes in plants and their tolerance to abiotic stresses. However, literature available on NO or H2S stand alone, and the major insights into the roles of NO and/or H2S in plant tolerance, particularly to metals, remained unclear. Given above, this paper aimed to (a) briefly overview metals and highlight their major phytotoxicity; (b) appraises literature reporting potential mechanisms underlying the roles of NO and H2S in plant-metal tolerance; (c) crosstalk on NO and H2S in relation to plant metal tolerance. Additionally, major aspects so far unexplored in the current context have also been mentioned.

Mechanisms and Role of Nitric Oxide in Phytotoxicity-Mitigation of Copper.

Phytotoxicity of metals significantly contributes to the major loss in agricultural productivity. Among all the metals, copper (Cu) is one of essential metals, where it exhibits toxicity only at its supra-optimal level. Elevated Cu levels affect plants developmental processes from initiation of seed germination to the senescence, photosynthetic functions, growth and productivity. The use of plant growth regulators/phytohormones and other signaling molecules is one of major approaches for reversing Cu-toxicity in plants. Nitric oxide (NO) is a versatile and bioactive gaseous signaling molecule, involved in major physiological and molecular processes in plants. NO modulates responses of plants grown under optimal conditions or to multiple stress factors including elevated Cu levels. The available literature in this context is centered mainly on the role of NO in combating Cu stress with partial discussion on underlying mechanisms. Considering the recent reports, this paper: (a) overviews Cu uptake and transport; (b) highlights the major aspects of Cu-toxicity on germination, photosynthesis, growth, phenotypic changes and nutrient-use-efficiency; (c) updates on NO as a major signaling molecule; and (d) critically appraises the Cu-significance and mechanisms underlying NO-mediated alleviation of Cu-phytotoxicity. The outcome of the discussion may provide important clues for future research on NO-mediated mitigation of Cu-phytotoxicity.

Nitric Oxide Pre-Treatment Advances Seed Germination and Alleviates Copper-Induced Photosynthetic Inhibition in Indian Mustard.

This investigation tested the efficiency of nitric oxide (NO) in alleviation of Cu-induced adverse impacts on seed germination and photosynthesis in Indian mustard (Brassica juncea L.). Pre-treatment of B. juncea seeds with sodium nitroprusside (SNP; NO donor) significantly improved the seed germination rate and also alleviated Cu-accrued oxidative stress. However, in the absence of NO, Cu caused a higher reduction in seed germination rate. The presence of NO strengthened the antioxidant defense system (glutathione reductase, ascorbate peroxidase, and superoxide dismutase) and thereby sustained the lower lipid peroxidation, reduced H2O2 content, and thiobarbituric acid reactive substances in Cu-exposed seeds. NO pre-treated seeds also retained a higher amylase activity and exhibited an improved seed germination rate. This effect of NO under Cu stress was also seen in plants originated from the NO pre-treated seeds, where the role of NO pre-treatment was reflected in the improved photosynthetic potential of B. juncea. Overall, NO pre-treatment not only improved the germination rate in seeds but also carried its effects in the grown seedlings evidenced as improved photosynthesis and growth. Potential mechanisms involved in the action of NO pre-treatment included NO-mediated significant strengthening of the antioxidant defense system and decreases in Cu-caused oxidative stress parameters.

Assessment of Antioxidants in Selected Plant Rootstocks.

The service tree (Sorbus domestica) is a wild fruit tree with immense medicinal and industrial value. This study aimed at determining the four major groups of antioxidants (flavonoids, phenolic acids and aldehydes, catechin and procyanidin) in rootstocks of Crataegus laevigata (genotypes O-LE-14 and O-LE-21), Aronia melanocarpa (genotypes O-LE-14 and O-LE-21), Chaenomeles japonica (genotype O-LE-9) and Cydonia oblonga (BA 29) (genotypes O-LE-14 and O-LE-21). Hyperoside (Quercetin 3-D-galactoside) was the most abundant flavonoid compound, since its average content in the rootstocks of Crataegus laevigata (O-LE-21) was 180.68 ± 0.04 µg·g-1. Dihydrokaempherol was the least frequently found flavonoid compound, with an average concentration of 0.43 ± 0.01 µg·g-1 in all the rootstocks of plants considered in this study. Among the phenolic compounds, the most represented one was protocatechuic acid, with 955.92 ± 10.25 µg·g-1 in the rootstocks of Aronia melanocarpa (O-LE-14). On the other hand, the least represented p-Coumaric acid exhibited the average concentration of 0.34 ± 0.01 µg·g-1 in the plant rootstocks. Epicatechin was the most abundant catechin compound, with a content of 3196.37 ± 50.10 µg·g-1 in the rootstocks of Aronia melanocarpa (O-LE-14). The lowest represented catechin compound was epigallocatechin, with the average concentration of 0.95 ± 0.08 µg·g-1 in the screened plant rootstocks. From the procyanidin compounds, the most abundant one was procyanidin b2 in the rootstocks of Crataegus laevigata (O-LE-14), with a concentration of 5550.40 ± 99.56 µg·g-1. On the contrary, procyanidin a2, with an average concentration of 40.35 ± 1.61 µg·g-1, represented the least frequent procyanidin compound in all the plant rootstocks screened herein.

Real-time monitoring of glutathione in living cells using genetically encoded FRET-based ratiometric nanosensor.

Reduced glutathione (GSH) level inside the cell is a critical determinant for cell viability. The level of GSH varies across the cells, tissues and environmental conditions. However, our current understanding of physiological and pathological GSH changes at high spatial and temporal resolution is limited due to non-availability of practicable GSH-detection methods. In order to measure GSH at real-time, a ratiometric genetically encoded nanosensor was developed using fluorescent proteins and fluorescence resonance energy transfer (FRET) approach. The construction of the sensor involved the introduction of GSH binding protein (YliB) as a sensory domain between cyan fluorescent protein (CFP; FRET donor) and yellow fluorescent protein (YFP; FRET acceptor). The developed sensor, named as FLIP-G (Fluorescence Indicator Protein for Glutathione) was able to measure the GSH level under in vitro and in vivo conditions. When the purified FLIP-G was titrated with different concentrations of GSH, the FRET ratio increased with increase in GSH-concentration. The sensor was found to be specific for GSH and also stable to changes in pH. Moreover, in live bacterial cells, the constructed sensor enabled the real-time quantification of cytosolic GSH that is controlled by the oxidative stress level. When expressed in yeast cells, FRET ratio increased with the external supply of GSH to living cells. Therefore, as a valuable tool, the developed FLIP-G can monitor GSH level in living cells and also help in gaining new insights into GSH metabolism.

Reactive oxygen species detection-approaches in plants: Insights into genetically encoded FRET-based sensors.

The generation of reactive oxygen species (ROS) (and their reaction products) in abiotic stressed plants can be simultaneous. Hence, it is very difficult to establish individual roles of ROS (and their reaction products) in plants particularly under abiotic stress conditions. It is highly imperative to detect ROS (and their reaction products) and ascertain their role in vivo and also to point their optimal level in order to unveil exact relation of ROS (and their reaction products) with the major components of ROS-controlling systems. Förster Resonance Energy Transfer (FRET) technology enables us with high potential for monitoring and quantification of ROS and redox variations, avoiding some of the obstacles presented by small-molecule fluorescent dyes. This paper aims to: (i) introduce ROS and overview ROS-chemistry and ROS-accrued major damages to major biomolecules; (ii) highlight invasive and non-invasive approaches for the detection of ROS (and their reaction products); (iii) appraise literature available on genetically encoded ROS (and their reaction products)-sensors based on FRET technology, and (iv) enlighten so far unexplored aspects in the current context. The studies integrating the outcomes of the FRET-based ROS-detection approaches with OMICS sciences (genetics, genomics, proteomics, and metabolomics) would enlighten major insights into real-time ROS and redox dynamics, and their signaling at cellular and subcellular levels in living cells.

Ageing-induced changes in nutritional and anti-nutritional factors in cowpea (Vigna unguiculata L.).

This study examined the role of seed ageing in the control of anti-nutritional factors in cowpea (Vigna unguiculata L.). In differently aged seeds of three cultivars of V. unguiculata (V240, V78 and V585), germination ability and vigour were studied. Effort was also made to assay trypsin inhibitor, phenol and phytic acid, perform protein profiling in these seeds. High vigour lots (V240 and V585) registered maximum increases in germination of aged seeds. The contents of nutritional factors such as total protein and carbohydrate declined with decrease in seed vigour lots. Anti-nutritional factors such as phytic acid, phenolic content and trypsin inhibitor activity decreased and varied in low and high vigour seed lots. Polypeptide banding pattern significantly varied in the high, medium and low vigour seeds. Notably, proteins with the highest relative mobility of 0.98 and lowest molecular weight of 11.5 kDa and lowest relative mobility of 0.17 and highest molecular weight of 102.0 kDa were observed in all the vigour lots. Results implied the decline in vigour of V. unguiculata seeds under conditions of controlled ageing can be related to the decline in content of major nutritional factors (total carbohydrates and proteins) required for the growing embryo during seed germination. Additionally, decreases in the contents of anti-nutritional factors phytic acid and phenols, and the activity of trypsin inhibitor in particular are connected with the decrease in seed vigour irrespective of V. unguiculata cultivars. The use of short duration controlled ageing technique can, at least partially, reduce the negative effects of anti-nutritional factors, and eventually improve the nutritional quality of V. unguiculata seeds.

Genome-wide identification and expression profiling of EIL gene family in woody plant representative poplar (Populus trichocarpa).

This study aimed to improve current understanding on ethylene-insensitive 3-like (EIL) members, least explored in woody plants such as poplar (Populus trichocarpa Torr. & Grey). Herein, seven putative EIL members were identified in P. trichocarpa genome and were roughly annotated either as EIN3-like sequence associated with ethylene pathway or EIL3-like sequences related with sulfur (S)-pathway. Motif-distribution pattern of proteins also corroborated this annotation. They were distributed on six chromosomes (chr1, 3, 4 and 8-10), and were revealed to encode a protein of 509-662 residues with nuclear localization. The presence of ethylene insensitive 3 (EIN3; PF04873) domain (covering first 80-280 residues from N-terminus) was confirmed by Hidden Markov Model-based search. The first half of EIL proteins (∼80-280 residues including EIN3 domain) was substantially conserved. The second half (∼300-600 residues) was considerably diverged. Additionally, first half of proteins harbored acidic, proline-rich and glutamine-rich sites, and supported the essentiality of these regions in the transcriptional-activation and protein-function. Moreover, identified six segmental and one-tandem duplications demonstrated the negative or purifying selective nature of mutations. Furthermore, expression profile analysis indicated the possibility of a crosstalk between EIN3- and EIL3-like genes, and co-expression networks implicated their interactions with very diverse panels of biological molecules.