Real-Time Measurement of Refractive Index Using 3D-Printed Optofluidic Fiber Sensor.

This work describes a 3D-printed optofluidic fiber sensor to measure refractive index in real time, combining a microfluidic system with an optical fiber extrinsic Fabry-Perot interferometer. The microfluidic chip platform was developed for this purpose through 3D printing. The Fabry-Perot cavity was incorporated in the microfluidic chip perpendicularly to the sample flow, which was of approximately 3.7 µL/s. The optofluidic fiber sensor platform coupled with a low-cost optical power meter detector was characterized using different concentrations of glucose solutions. In the linear regression analysis, the optical power shift was correlated with the refractive index and a sensitivity of -86.6 dB/RIU (r2 = 0.996) was obtained. Good results were obtained in terms of stability with a maximum standard deviation of 0.03 dB and a sensor resolution of 5.2 × 10-4 RIU. The feasibility of the optofluidic fiber sensor for dynamic analyses of refractive index with low sample usage was confirmed through real-time measurements.

Fiber Bragg Grating Sensors for Reinforcing Bar Slippage Detection and Bond-Slip Gradient Characterization.

The detection of bond-slip between the reinforcing bar (RB) and concrete is of great importance to ensure the safety of reinforced concrete (RC) structures. The techniques to monitor the connection between the RB and concrete are in constant development, with special focus on the ones with straightforward operation and simple non-intrusive implementation. In this work, a simple configuration is developed using 10 optical fiber sensors, allowing different sections of the same RC structure to be monitored. Since the RB may suffer different strains along its length, the location of the sensors is critical to provide an early warning about any displacement. Bragg gratings were inscribed in both silica and polymer optical fibers and these devices worked as displacement sensors by monitoring the strain variations on the fibers. The results showed that these sensors can be easily implemented in a civil construction environment, and due to the small dimensions, they can be a non-intrusive technique when multiple sensors are implemented in the same RC structure.

Sensing System Based on FBG for Corrosion Monitoring in Metallic Structures.

As corrosion has slow development, its detection at an early age could be an alternative for reducing costs of structural rehabilitation. Therefore, the employment of structural health monitoring (SHM) systems, sensing configurations collecting data over time allowing for observing changes in the properties of the materials and damage emergence, for monitoring corrosion can be a good strategy to measure the damage and to decide the better moment for intervention. Nonetheless, the current corrosion sensor technology and the high costs of the sensing system implementation are limiting this application in the field. In this work, an optical fiber Bragg grating (FBG)-based sensing system is proposed for monitoring the thickness loss of a 1020 carbon steel metal plate subjected to controlled corrosion. The natural frequency of the plate was collected as a function of the corrosion time over 3744 h. To validate the experimental results, ultrasound measures and electrochemical tests were also carried out under similar conditions. The experimental results show adequate reliability, indicating the suitable functionality of the proposed system for monitoring the thickness loss caused by corrosion in metallic structures, in comparison with traditional methods, as ultrasonic and electrochemical measures.

Lateral flow assay applied to pesticides detection: recent trends and progress.

Devices based on lateral flow assay (LFA) have been gaining more and more space in the detection market mainly due to their simplicity, speed, and low cost. These devices have excellent sensing format versatility and make these strips an ideal choice for field applications. The COVID-19 pandemic boosted the democratization of this method as a "point of care testing" (POCT), and the trend is that these devices become protagonists for the monitoring of pesticides in the environment. However, designing LFA devices for detecting and monitoring pesticides in the environment is still a challenge. This is because analytes are small molecules and have only one antigenic determinant, which makes it difficult to apply direct immunoassays. Furthermore, most LFA devices provide only qualitative or semi-quantitative results and have a limited number of applications in multi-residue analysis. Here, we present the state of the art on the use of LFA in the environmental monitoring of pesticides. Based on well-documented results, we review all available LFA formats and strategies for pesticide detection, which may have important implications for the future of monitoring pesticides in the environment. The main advances, challenges, and perspectives of these devices for a direction in this field of study are also presented.

Respiratory and heart rate monitoring using an FBG 3D-printed wearable system.

This work proposes a 3D-printed sensor based on fiber Bragg grating (FBG) technology for respiratory rate (RR) and heart rate (HR) monitoring. Each sensor is composed of a single FBG fully encapsulated into a 3D-printable Flexible, during the printing process. Sensors with different material thicknesses and infill densities were tested. The sensor with the best metrological properties was selected and preliminary assessed in terms of capability of monitoring RR and HR on three users. Preliminary results proved that the developed sensor can be a valuable easy-to-fabricate solution, with high reproducibility and high strain sensitivity to chest wall deformations due to breathing and heart beating.

Sensor Cell Network for Pressure, Temperature and Position Detection on Wheelchair Users.

This work proposes an optical sensing network to monitor pressure and temperature in specific areas of a wheelchair to prevent pressure ulcers and to monitor the position of the wheelchair user by analyzing its pressure distribution. The sensing network is composed of six optical fiber Bragg grating (FBG)-based sensor cells. Each sensor cell is built from a polylactic acid (PLA) base and has two FBGs, one embedded in epoxy resin to monitor pressure variations (FBGP) and another without resin to monitor temperature (FBGT). Once produced, all sensor cells were experimentally characterized for pressure and temperature variations, resulting in an average pressure sensitivity of 81 ± 5 pm/kPa (FBGP) and -5.0 ± 0.4 pm/kPa (FBGT), and an average temperature sensitivity of 25 ± 1 pm/°C (FBGP) and 47.7 ± 0.7 pm/°C (FBGT). The sensor cells were then placed in six specific areas of a wheelchair (four in the seat area and two in the shoulder blade area) to carry out experimental tests, wherein the response of the sensors to a specific sequence of relief positions was tested. During the execution of the test, the optical signal of all sensors was monitored, in real time, with the pressure and temperature values detected in each zone of the wheelchair. In addition, random position changes were performed in order to evaluate the precision of the proposed sensing network in the identification of such positions.

Mixed laryngocele mimicking thyroid nodule.

Laryngocele is defined as a dilation of the laryngeal saccule forming an air sac. Some differential diagnoses for laryngocele have been reported. The aim of the present paper was to describe a case of a patient referred for the evaluation a suspected thyroid nodule that was subsequently diagnosed as a mixed laryngocele. A 31-year-old male with no clinical manifestations was referred by an endocrinologist to undergo Doppler ultrasonography and fine-needle aspiration biopsy due to a preliminary ultrasonographic diagnosis of a nodule apparently in the thyroid. The diagnosis of laryngocele was raised considering the cytopathological analysis and imaging exam. Computed tomography of the neck confirmed this hypothesis. The patient was counseled to consult a surgeon, but, up to the end of this report, continued asymptomatic and in follow-up. We report a mixed laryngocele with different clinical behavior, showing that laryngocele may appear to be another entity and drawing the attention of clinicians to imaging similarities.

Interrogation Method with Temperature Compensation Using Ultra-Short Fiber Bragg Gratings in Silica and Polymer Optical Fibers as Edge Filters.

The use of simpler and less bulky equipment, with a reliable performance and at relative low cost is increasingly important when assembling sensing configurations for a wide variety of applications. Based on this concept, this paper proposes a simple, efficient and relative low-cost fiber Bragg grating (FBG) interrogation solution using ultra-short FBGs (USFBGs) as edge filters. USFBGs with different lengths and reflection bandwidths were produced in silica optical fiber and in poly(methyl methacrylate) (PMMA) microstructured polymer optical fiber (mPOF), and by adjusting specific inscription parameters and the diffraction pattern, these gratings can present self-apodization and unique spectral characteristics suitable for filtering operations. In addition to being a cost-effective edge filter solution, USFBGs and standard uniform FBGs in silica fiber have similar thermal sensitivities, which results in a straightforward operation without complex equipment or calculations. This FBG interrogation configuration is also quite promising for dynamic measurements, and due to its multiplexing capabilities multiple USFBGs can be inscribed in the same optical fiber, allowing to incorporate several filters with identical or different spectral characteristics at specific wavelength regions in the same fiber, thus showing great potential to create and develop new sensing configurations.

Fiber Optic Load Cells with Enhanced Sensitivity by Optical Vernier Effect.

Developing technologies capable of constantly assessing and optimizing day-to-day activities has been a research priority for several years. A key factor in such technologies is the use of highly sensitive sensors to monitor in real-time numerous parameters, such as temperature and load. Due to their unique features, optical fiber sensors became one of the most interesting and viable solutions for applications dependent on those parameters. In this work, we present an optical fiber load sensor, called load cell, based on Fabry-Pérot hollow cavities embedded in a polymeric material. By using the load cells in a parallel configuration with a non-embedded hollow cavity, the optical Vernier effect was generated, allowing maximum sensitivity values of 0.433 nm N-1 and 0.66 nm °C-1 to be attained for vertical load and temperature, respectively. The proposed sensor's performance, allied with the proposed configuration, makes it a viable and suitable device for a wide range of applications, namely those requiring high thermal and load sensitivities.

An Optimized Self-Compensated Solution for Temperature and Strain Cross-Sensitivity in FBG Interrogators Based on Edge Filter.

Optical fiber sensors based on fiber Bragg gratings (FBGs) are prone to measurement errors if the cross-sensitivity between temperature and strain is not properly considered. This paper describes a self-compensated technique for canceling the undesired influence of temperature in strain measurement. An edge-filter-based interrogator is proposed and the central peaks of two FBGs (sensor and reference) are matched with the positive and negative slopes of a Fabry-Perot interferometer that acts as an optical filter. A tuning process performed by the grey wolf optimizer (GWO) algorithm is required to determine the optimal spectral characteristics of each FBG. The interrogation range is not compromised by the proposed technique, being determined by the spectral characteristics of the optical filter in accordance with the traditional edge-filtering interrogation. Simulations show that, by employing FBGs with optimal characteristics, temperature variations of 30 °C led to an average relative error of 3.4% for strain measurements up to 700µÏµ. The proposed technique was experimentally tested under non-ideal conditions: two FBGs with spectral characteristics different from the optimized results were used. The temperature sensibility decreased by 50.8% as compared to a temperature uncompensated interrogation system based on an edge filter. The non-ideal experimental conditions were simulated and the maximum error between theoretical and experimental data was 5.79%, proving that the results from simulation and experimentation are compatible.

Microscale sensor solution for data collection from fibre-matrix interfaces.

Especially the applications of fibrous composites in miniature products, dental and other medical applications require accurate data of microscale mechanics. The characterization of adhesion between single filament and picoliter-scale polymer matrix usually relies on the experiments using so-called microbond (MB) testing. The traditional MB test systems provide unitary data output (i.e., converted force) which is enigmatic in resolving the fracture parameters of multi-mode interface cracks. As a fundamental basis, the momentary reaction force and respective local strain at the location of a non-ambiguous gradient are needed for a mechanical analysis. In this paper, a monolithic compliant based structure with an integrated Fiber Bragg Grating (FBG) sensor is developed and analysed. The stiffness of the compliant structure is estimated by using mathematical and finite element (FE) models. Qualification experiments are carried out to confirm the functional performance: MB testing of synthetic (carbon and glass) and natural (flax) single filaments are successfully performed. Quasi-static and dynamic analysis of the MB testing is carried out by using the FE method to interpret the response of the compliant structure. The developed strain-sensing CBPM-FBG holder shows excellent sensitivity during the MB tests for both synthetic and natural filaments, even at a low filament diameters as low as [Formula: see text], making the monolithic compliant structure the first instrument capable of force-strain data output for bonded filament-droplet specimens.

Polymer optical fibers for mechanical wave monitoring.

This Letter presents the development of a low-cost polymer optical fiber (POF) sensor for mechanical wave monitoring. The POF is fabricated using the light polymerization spinning process (LPS-POF) with Bisphenol-A as its main component, resulting in a highly flexible fiber. The proposed LPS-POF sensor is applied on the assessment of squared waves with different amplitudes, where the amplitude and dynamic responses are compared to the ones of a piezoelectric transducer (PZT). In static conditions, a determination coefficient (R2) of 0.990 is obtained between the reference (PZT) and proposed sensors for the amplitude assessment of the wave. In dynamic analysis, the LPS-POF viscoelasticity is compensated using viscoelastic constitutive models, resulting in a R2 of 0.988 between the sensor responses, which indicate a mean error reduction of 21% when compared to the uncompensated responses in the amplitudes of different square waves. The dynamic analysis also shows the sensor capability of operating in frequencies as high as 25 Hz. Then, the sensor's responses, compared to the input squared wave, show the possibility of wave velocity measurement. Therefore, with a LPS-POF sensor array, it is possible to monitor these parameters in practical applications.

Performance Analysis of Scattering-Level Multiplexing (SLMux) in Distributed Fiber-Optic Backscatter Reflectometry Physical Sensors.

Optical backscatter reflectometry (OBR) is a method for the interrogation of Rayleigh scattering occurring in each section of an optical fiber, resulting in a single-fiber-distributed sensor with sub-millimeter spatial resolution. The use of high-scattering fibers, doped with MgO-based nanoparticles in the core section, provides a scattering increase which can overcome 40 dB. Using a configuration-labeled Scattering-Level Multiplexing (SLMux), we can arrange a network of high-scattering fibers to perform a simultaneous scan of multiple fiber sections, therefore extending the OBR method from a single fiber to multiple fibers. In this work, we analyze the performance and boundary limits of SLMux, drawing the limits of detection of N-channel SLMux, and evaluating the performance of scattering-enhancement methods in optical fibers.

Indigenous microalgae biomass cultivation in continuous reactor with anaerobic effluent: effect of dilution rate on productivity, nutrient removal and bioindicators.

Effluents from municipal wastewater treatment have been long recognized as suitable media for the cultivation of microalgae biomass. However, few studies report data concerning biomass productivity in continuous reactors using unsterilized wastewater effluents. This study focuses on indigenous microalgae strains that grow with native bacteria and are applicable for biomass production and tertiary wastewater treatment in continuous growth mode. Initially, five Chlorophyta strains were isolated and grown in batch mode to single out a potential inoculum for the experiments in continuous growth mode. The isolate Chlorella sp. L06 was selected and evaluated based on five dilution rates from 0.1 to 0.5 day-1 on continuous growth reactor using unsterilized secondary effluent as culture medium. Maximal volumetric biomass productivity of 283 mg L-1 day-1 was achieved at 0.3 day-1 without CO2 addition or air bubbling. Carbohydrates were the major fraction of the dried biomass, followed by proteins and then lipids. The highest removal rates of total nitrogen and phosphorus from the liquid phase were 13.0 and 1.4 mg L-1 day-1, respectively, and were obtained at 0.4 day-1. The maximal decay rate for E. coli (2.9 day-1) was achieved both at 0.3 and 0.4 day-1. Conclusively, Chlorella sp. L06 cultivation in unsterilized secondary effluent can be adjusted depending on the objective: for biomass production, a dilution rate of approximately 0.3 day-1 is recommended; and for tertiary treatment a rate of 0.4 day-1 is suggested.

Inscription of Bragg gratings in undoped PMMA mPOF with Nd:YAG laser at 266†nm wavelength.

We present the first Bragg gratings fabricated in two, three and five rings undoped PMMA microstructured polymer optical fibres (mPOFs) with relative low cost 266 nm Nd:YAG laser in the 850 nm region. The fibers were connectorised with commercial ferrules for easy coupling with silica patch cables. Temperature, humidity and strain sensitivities are measured and also the impact of ring structure and the diameter of POF on the characterization measurements are studied for potential applications. We also analyzed the effect of the number of hexagonal rings structure in gratings fabrication, noticing that larger number of rings lead to more difficulties to obtain strong gratings, where we consider this performance due to the scattering effects. We demonstrate Bragg gratings fabrication in 5-rings structure mPOF after 6 min by using 266 nm Nd:YAG laser whereas no Bragg gratings have been fabricated so far using 325 nm He-Cd laser system. Up to 30 dB relative reflected power gratings are obtained in two rings mPOF, showing good time stability and promising results for undoped mPOF applications.

High Rate Dynamic Monitoring with Fabry-Perot Interferometric Sensors: An Alternative Interrogation Technique Targeting Biomedical Applications.

Fabry-Perot interferometric (FPI) sensors are an accurate and well-established sensing technology that are used to monitor a wide range of parameters such as strain, temperature, and refractive index, among many others. Nevertheless, due to the limited number and high cost of existing interrogation techniques for FPIs, its use is often restricted to discrete measurements, not being so explored for dynamic applications. The development of an alternative interrogation technique for a high rate of acquisition may propel this type of sensor into less explored fields such as dynamic biomedical applications. In this work, we present the theoretical and experimental analyses of an FPI sensing architecture by using an alternative high rate dynamic acquisition methodology, based on frequency to amplitude conversion, where the FPI spectral shift is detuned by the convolution of the optical light source with the FPI interference pattern. The good agreement between the theoretical and experimental results verified the reliability of the proposed methodology. Moreover, preliminary results show that the developed sensing architecture can be a suitable solution to monitor biomedical parameters such as the carotid pulse wave.

Highly sensitive fiber optic temperature and strain sensor based on an intrinsic Fabry-Perot interferometer fabricated by a femtosecond laser.

In this Letter, we report on a simple, but highly sensitive sensor based on two intrinsic Fabry-Perot interferometers (FPIs) inscribed in a standard single-mode optical fiber. A brief theoretical study on the Vernier effect is presented, in which a simulation of the sensitivity magnification factor dependence on the FPI's length is performed. Based on the simulation results, the FPIs were fabricated using a custom micromachining setup that integrates a near-infrared femtosecond laser and a motorized XYZ platform. Using the Vernier effect, sensitivities of 145 pm/µÎµ and 927 pm/°C were obtained for strain and temperature, respectively. The sensor's performance combined with its versatile and customizable configuration allows real-time and in situ strain and temperature monitoring under harsh environments.

Impact of conjugation strategies for targeting of antibodies in gold nanoparticles for ultrasensitive detection of 17ß-estradiol.

Antibody-coated nanoparticles have recently attracted considerable attention, with the focus falling on diagnostics. Nevertheless, controlled antibody bioconjugation remains a challenge. Here, we present two strategies of bioconjugation with the aim of evaluating the best approach for the coupling of antibodies on the surface of nanomaterials in an oriented way. We employed electrostatic interaction (physical adsorption) and covalent conjugation in the orientation of antibodies on the metallic surface as coupling methods, and their influence on the detection of 17ß-estradiol was addressed with localized surface plasmon resonance. The understanding of these mechanisms is fundamental for the development of reproducible inorganic bioconjugates with oriented surface as well sensibility of immunoassays.

Perrogator: A Portable Energy-Efficient Interrogator for Dynamic Monitoring of Wavelength-Based Sensors in Wearable Applications.

In this paper, we report the development of a portable energy-efficient interrogator (Perrogator) for wavelength-based optical sensors. The interrogator is based on a compact solution encompassing a white light source and the spectral convolution between the sensor and a tunable filter, which is acquired by a photodetector, where a microcontroller has two functions: (i) To control the filter tuning and to (ii) acquire the photodetector signal. Then, the data is sent to a single-board computer for further signal processing. Furthermore, the employed single-board computer has a Wi-Fi module, which can be used to send the sensors data to the cloud. The proposed approach resulted in an interrogator with a resolution as high as 3.82 pm (for 15.64 nm sweeping range) and maximum acquisition frequency of about 210 Hz (with lower resolution ~15.30 pm). Perrogator was compared with a commercial fiber Bragg grating (FBG) interrogator for strain measurements and good agreement between both devices was found (1.226 pm/µÎµ for the commercial interrogator and 1.201 pm/µÎµ for the proposed approach with root mean square error of 0.0144 and 0.0153, respectively), where the Perrogator has the additional advantages of lower cost, higher portability and lower energy consumption. In order to demonstrate such advantages in conjunction with the high acquisition frequency allowed us to demonstrate two wearable applications using the proposed interrogation device over FBG and Fabry-Perot interferometer (FPI) sensors. In the first application, an FBG-embedded smart textile for knee angle assessment was used to analyze the gait of a healthy person. Due to the capability of reconstructing the FBG spectra, it was possible to employ a technique based on the FBG wavelength shift and reflectivity to decouple the effects of the bending angle and axial strain on the FBG response. The measurement of the knee angle as well as the estimation of the angular and axial displacements on the grating that can be correlated to the variations of the knee center of rotation were performed. In the second application, a FPI was embedded in a chest band for simultaneous measurement of breath and heart rates, where good agreement (error below 5%) was found with the reference sensors in all analyzed cases.