Assuntos
Interferon-alfa/farmacologia , Anticorpos Monoclonais/farmacologia , Contagem de Células , Divisão Celular/efeitos dos fármacos , Linhagem Celular , Estabilidade de Medicamentos , Armazenamento de Medicamentos , Citometria de Fluxo , Humanos , Interferon alfa-2 , Interferon-alfa/química , Testes de Neutralização , Proteínas Recombinantes , Albumina Sérica/química , Albumina Sérica/farmacologiaRESUMO
The L-selectin leukocyte adhesion molecule plays an important role in controlling leukocyte extravasation in peripheral lymph nodes and at sites of tissue injury or infection. Although febrile responses during infection and inflammation are associated with enhanced immune activity, the contribution of fever-range temperatures to controlling lymphocyte recruitment to tissues has not been previously examined. In this report we provide evidence that direct exposure of lymphocytes to fever-range temperatures (38-41 degrees C) in vitro for 9 to 24 h resulted in a >100% increase in L-selectin-dependent adhesion of these cells to lymph node high endothelial venules (HEV). Moreover, culture of lymphocytes under hyperthermia conditions markedly enhanced the ability of these cells to traffic in an L-selectin-dependent manner to peripheral lymph nodes, mesenteric lymph nodes, and Peyer's patches. In contrast, febrile temperatures did not increase LFA-1 function as assessed by measuring lymphocyte adhesion to ICAM-1-3T3 transfectants. Fever-range hyperthermia further did not increase L-selectin surface density on lymphocytes or L-selectin-dependent recognition of soluble carbohydrate substrates; however, a marked increase in ultrastructural immunogold-labeling of L-selectin was observed in response to thermal stimuli. These results suggest that elevated temperatures enhance L-selectin adhesion and/or avidity through the regulation of L-selectin conformation or organization in the plasma membrane. Finally, the observed thermal effects on L-selectin adhesion were attributed to soluble factors in the conditioned medium of heat-treated cells. Taken together, these data provide new insight into the potential physiologic role of the febrile response in enhancing lymphocyte recruitment to tissues through the regulation of L-selectin adhesion.
Assuntos
Febre/imunologia , Selectina L/fisiologia , Linfócitos/imunologia , Linfócitos/fisiologia , Adesão Celular/imunologia , Movimento Celular/imunologia , Células Cultivadas , Endotélio Linfático/citologia , Endotélio Linfático/imunologia , Humanos , Selectina L/biossíntese , Selectina L/imunologia , Ligantes , Linfonodos , Linfócitos/metabolismo , Mananas/metabolismo , Manosefosfatos/metabolismo , Solubilidade , Fatores de TempoRESUMO
The L-selectin adhesion molecule mediates lymphocyte extravasation in peripheral lymph nodes, and has also been implicated in directing leukocyte recruitment to inflammatory tissues and metastasis of lymphoid malignancies. In this study, we demonstrate a novel level of regulation of L-selectin expression that involves the 16-kDa Leu-13 signal transduction molecule. Leu-13 is a member of a multimeric cell surface complex in lymphocytes that includes TAPA-1 (target of antiproliferative Ab-1, CD81) as well as lineage-specific proteins. In the present study, mAb-induced ligation of Leu-13 was shown to rapidly down-regulate L-selectin surface density on normal and malignant human lymphocytes, and to markedly inhibit L-selectin-mediated adhesion of lymphocytes to soluble carbohydrate ligands (i.e., PPME, phosphomonoester core polysaccharide) and to lymph node high endothelial venules. Through the use of genistein and staurosporine, potent inhibitors of tyrosine kinases (TK) and protein kinase C (PKC), respectively, Leu-13-induced L-selectin down-modulation was demonstrated to involve a TK-dependent, PKC-independent pathway, and was attributed to increased L-selectin shedding from surface membranes. Notably, direct L-selectin ligation, modeling cross-linking interactions with endothelial cell ligands, similarly down-regulates L-selectin surface expression through a TK-dependent, PKC-independent mechanism. In sharp contrast, PMA and anti-CD3 mAb down-regulate L-selectin via a staurosporine-sensitive, genistein-resistant pathway that is closely linked to lymphocyte proliferation. Taken together, these results demonstrate a novel role for Leu-13- and L-selectin-induced TK activity in control of L-selectin expression, thus providing insight into the complex molecular mechanisms that potentially regulate L-selectin-dependent lymphocyte homing in vivo.
Assuntos
Antígenos de Diferenciação/metabolismo , Selectina L/biossíntese , Leucócitos Mononucleares/metabolismo , Proteína Quinase C/metabolismo , Proteínas Tirosina Quinases/metabolismo , Transdução de Sinais , Citometria de Fluxo , HumanosRESUMO
Calcium channel blockers cause antiproliferative effects on various cells in culture. Since angioneogenesis is a crucial step in the development of tumor growth, we examined the influence of different calcium channel blockers on human umbilical arterial endothelial cell (HUAEC) growth. Cell growth was measured by cell count, by [3H]thymidine incorporation, and by a 5-bromo-2-deoxyuridine (BrdU-incorporation immunofluorescence assay. Cell-cycle analysis was performed by flow cytometric analysis. Nifedipine, isradipine, diltiazem, and verapamil dose-dependently inhibited the basic fibroblast growth factor (bFGF)-induced [3H]thymidine incorporation. Fifty micromolars of nifedipine, isradipine, diltiazem, and verapamil completely inhibited bFGF-induced proliferation of HUAEC. Ten micromolars of each calcium channel blocker abolished the bFGF-induced increase in cell count. Five micromolars of isradipine completely blocked the bFGF-induced BrdU incorporation. Stimulation of HUAEC with bFGF (50 ng/ml) for 24 h caused a 2-fold increase in cells that entered S and G2+M phase in comparison with control cells. Five micromolars of isradipine abolished this effect completely. We conclude that calcium channel blockers are able to inhibit cell proliferation by a cell-cycle arrest in G0/G1 phase.
Assuntos
Bloqueadores dos Canais de Cálcio/farmacologia , Ciclo Celular , Di-Hidropiridinas/farmacologia , Endotélio Vascular/citologia , Inibidores do Crescimento/farmacologia , Substâncias de Crescimento/farmacologia , DNA/biossíntese , Fator de Crescimento Epidérmico/farmacologia , Fator 1 de Crescimento de Fibroblastos/farmacologia , Fator 2 de Crescimento de Fibroblastos/farmacologia , Citometria de Fluxo , HumanosRESUMO
In the present study, the effect of 10(-9) to 10(-6) M epinephrine (alpha- and beta-agonist), norepinephrine (alpha- and beta 1-antagonist) isoproterenol (beta-agonist) salbutamol (beta 2-agonist), phenylephrine (alpha 1-agonist) and oxymetazoline (mainly alpha 2-agonist) on DNA synthesis in vascular smooth muscle cells (VSMCs) from rat aorta has been investigated. Our results show that only oxymetazoline induced a moderate dose-dependent elevation of [3H]thymidine incorporation into cell DNA (10(-6) M, 100-300%). Epidermal growth factor (EGF) (50 ng/ml) and platelet-derived growth factor (PDGF)-BB induced an elevation of the [3H]thymidine incorporation into cell DNA from 154 +/- 7 (basal value) to 1270 +/- 95 and 1552 +/- 178 cpm/microgram protein (mean +/- S.D., n = 3). Oxymetazoline (10(-6) M) and phenylephrine induced an increase of [3H]thymidine incorporation to 368 +/- 53 and 205 +/- 27 cpm/microgram protein, respectively. In contrast to phenylephrine, oxymetazoline caused an elevation of the PDGF-BB- and EGF-induced [3H]thymidine incorporation to 1561 +/- 143 and 2086 +/- 235 (means S.D., n = 3), respectively. In addition, EGF (1 to 50 ng/ml) induced a dose-dependent increase of [3H]thymidine incorporation from 154 +/- 7 (basal value) to 486 +/- 35 (1 ng/ml), 912 +/- 74 (5 ng/ml), 1019 +/- 40 (25 ng/ml) and 1270 +/- 95 (50 ng/ml) cpm/microgram protein (mean +/- S.D.). In the presence of 10(-6) M oxymetazoline, 1, 5, 25 and 50 ng/ml EGF caused an increase of [3H]thymidine incorporation to 633 +/- 101, 1124 +/- 87, 1231 +/- 101, and 1561 +/- 89 cpm/microgram protein (mean +/- S.D.).(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
DNA/biossíntese , Fator de Crescimento Epidérmico/farmacologia , Oximetazolina/farmacologia , Fator de Crescimento Derivado de Plaquetas/farmacologia , Agonistas Adrenérgicos/farmacologia , Animais , Aorta , Células Cultivadas , AMP Cíclico/biossíntese , Feminino , Músculo Liso Vascular/citologia , Músculo Liso Vascular/efeitos dos fármacos , Músculo Liso Vascular/metabolismo , Ratos , Ratos Endogâmicos WKY , Receptores Adrenérgicos beta 2/fisiologiaRESUMO
The L-selectin homing receptor expressed by lymphocytes mediates the initial attachment of these cells to high endothelial venules within peripheral lymph nodes. This adhesive interaction is required for the migration of B and T lymphocytes from the blood into peripheral lymph nodes. There is currently little information regarding the nature of the factors involved in the regulation of the synthesis and expression of L-selectin by lymphocytes. In this report, the immunomodulatory cytokine interferon-alpha (IFN-alpha) was shown to markedly upregulate the surface density of L-selectin in the established human B lymphoid Daudi cell line and in a subpopulation of tissue-derived human B lymphoid cells. Other cytokines such as IFN-gamma, tumor necrosis factor-alpha, interleukin (IL)-1 beta, IL-2, IL-4, IL-6, and low molecular weight B cell growth factor did not affect L-selectin surface expression in the model Daudi B cell line. Upregulation of L-selectin surface density in IFN-alpha-treated Daudi B cells correlated directly with an increase in L-selectin mRNA steady state levels and enhanced L-selectin-dependent binding to a carbohydrate-based ligand, phosphomonoester core polysaccharide. Regulation of L-selectin mRNA by IFN-alpha had characteristics similar to that of classical IFN-stimulated genes including rapid kinetics of induction, protein-synthesis-independent induction, and sensitivity to tyrosine-kinase inhibitors. IFN-alpha did not upregulate L-selectin mRNA levels or surface expression in an IFN-resistant Daudi subclone which exhibits a defect in the signal transduction pathway required for the transcriptional induction of IFN-stimulated genes. These data demonstrate a fundamental role for IFN-alpha in regulating L-selectin synthesis and expression in human B lymphoid cells and suggest a mechanism whereby this cytokine regulates the regional trafficking of B cells to peripheral lymph nodes.
Assuntos
Linfócitos B/metabolismo , Moléculas de Adesão Celular/biossíntese , Expressão Gênica/efeitos dos fármacos , Interferon Tipo I/farmacologia , Receptores de Retorno de Linfócitos/biossíntese , Linfócitos B/efeitos dos fármacos , Linfócitos B/imunologia , Sequência de Bases , Moléculas de Adesão Celular/análise , Linhagem Celular , Membrana Celular/metabolismo , Primers do DNA , Sondas de DNA , Relação Dose-Resposta a Droga , Citometria de Fluxo , Imunofluorescência , Humanos , Interferon gama/farmacologia , Interleucina-4/farmacologia , Interleucinas/farmacologia , Selectina L , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , RNA Mensageiro/análise , RNA Mensageiro/biossíntese , Proteínas Recombinantes/farmacologia , Transcrição Gênica/efeitos dos fármacos , Células Tumorais Cultivadas , Fator de Necrose Tumoral alfa/farmacologiaAssuntos
Proteínas de Ligação a DNA/genética , DNA/biossíntese , Proteínas Imediatamente Precoces , Lipoproteínas/farmacologia , Músculo Liso Vascular/efeitos dos fármacos , Músculo Liso Vascular/metabolismo , Fatores de Transcrição/genética , Animais , Cálcio/metabolismo , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Proteína 1 de Resposta de Crescimento Precoce , Expressão Gênica/efeitos dos fármacos , Humanos , Lipoproteínas HDL/farmacologia , Lipoproteínas LDL/farmacologia , Lipoproteínas VLDL/farmacologia , Músculo Liso Vascular/citologia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RatosRESUMO
Hypertension has a high prevalence among subjects with decreased insulin sensitivity and/or hyperinsulinemia. Furthermore, angiotensin II plays a pivotal role in the regulation of vascular tone and is known to induce hypertrophy and/or hyperplasia in vascular smooth muscle cells. In the present study, the effect of insulin on angiotensin II induced smooth muscle cell growth (Wistar-Kyoto rat) was investigated. Cell growth was assessed by the measurement of [3H]thymidine incorporation into cell DNA. Insulin in a concentration range of 1.7 x 10(-10)-1.7 x 10(-6) M lacked any effect on cell DNA synthesis. However, insulin enhanced the angiotensin II induced DNA synthesis in a concentration-dependent manner. This effect was similar in cells with a weak and in cells with a marked response in DNA synthesis to stimulation with 100 nM angiotensin II. In conclusion, insulin is able to enhance angiotensin II induced DNA synthesis and may therefore function as a growth cofactor in vascular smooth muscle cells.
Assuntos
Angiotensina II/farmacologia , DNA/biossíntese , Insulina/farmacologia , Músculo Liso Vascular/efeitos dos fármacos , Animais , Relação Dose-Resposta a Droga , Sinergismo Farmacológico , Feminino , Ratos , Ratos Endogâmicos WKYRESUMO
2-n-Butyl-4-chloro-5-hydroxymethyl-1-[(2'-(1H-tetrazol-5-yl)bip hen yl-4-yl)methyl]imidazole, potassium salt (Losartan) (previous name, DuP 753 or MK 954) is a nonpeptide angiotensin II receptor antagonist. This study was performed to investigate the ability of Losartan to inhibit the angiotensin II-induced stimulation of the phospoinositide signalling system and the angiotensin II-induced hypertrophy in aortic vascular smooth muscle cells of normotensive Wistar-Kyoto rats. 10(-7) M Losartan abolished the angiotensin II-induced formation of inositol 1,4,5-trisphosphate in vascular smooth muscle cells. 10(-6) M Losartan completely abolished the angiotensin II-induced elevation of the intracellular free Ca2+ concentration ([Ca2+]i). 10(-6) M Losartan lacked effects on the [Arg8]vasopressin-induced elevation of [Ca2+]i. In addition, 10(-6) M completely inhibited the angiotensin II-induced stimulation of Na+/H+ exchange in the vascular smooth muscle cells. 10(-10) to 10(-6) M Losartan inhibited the angiotensin II-induced cell protein synthesis in a concentration-dependent manner, yielding to an effective concentration (ED50) of 6.2 +/- 1.8 x 10(-8) M (n = 4). Losartan did not affect the platelet-derived growth factor-BB-induced increase in cell protein. These results show that Losartan is a highly specific angiotensin II receptor antagonist which inhibits angiotensin II-induced cell growth and thus may have beneficial effects on the development and regression of vascular hypertrophy.
Assuntos
Angiotensina II/antagonistas & inibidores , Compostos de Bifenilo/farmacologia , Imidazóis/farmacologia , Inositol 1,4,5-Trifosfato/metabolismo , Músculo Liso Vascular/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos , Tetrazóis/farmacologia , Angiotensina II/farmacologia , Animais , Aorta , Cálcio/metabolismo , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Losartan , Músculo Liso Vascular/citologia , Músculo Liso Vascular/metabolismo , Ratos , Ratos Endogâmicos WKYRESUMO
Structural changes within the blood vessel wall such as hyperplasia and hypertrophy of vascular smooth muscle cells are important factors in the pathogenesis of hypertension. Humoral growth factors such as angiotensin II (AII) and platelet-derived growth factor BB (PDGF-BB) may participate in the remodelling of the blood vessel wall. Whether and by which mechanisms antihypertensive treatment is capable of influencing the structural blood vessel alterations to date remains unclear. In the present study, the effect of nifedipine and diltiazem on AII- and PDGF-BB-induced vascular smooth muscle cell proliferation was examined. Nifedipine and diltiazem at a concentration of 10 microM did not affect baseline DNA synthesis in isolated vascular smooth muscle cells in culture. AII (final concentration 100 nM) and PDGF-BB (50 ng/ml) stimulated DNA synthesis by approximately 9.0- and 4.6-fold, respectively. Both AII- and PDGF-BB-induced DNA synthesis was significantly blunted by diltiazem and nifedipine in a concentration of 10 microM, while no significant influence was seen with concentrations from 10 nM up to 1 microM. In contrast, no significant influence of these drugs could be observed on fetal calf serum 5%-induced DNA synthesis. The findings indicate that calcium antagonists possess antimitogenic potential and that they may thus contribute to the regression of structural changes of the blood vessels associated with hypertension.
Assuntos
Angiotensina II/antagonistas & inibidores , Bloqueadores dos Canais de Cálcio/farmacologia , Divisão Celular/efeitos dos fármacos , Músculo Liso Vascular/efeitos dos fármacos , Fator de Crescimento Derivado de Plaquetas/antagonistas & inibidores , Angiotensina II/fisiologia , Animais , Divisão Celular/fisiologia , Células Cultivadas , Replicação do DNA/efeitos dos fármacos , Replicação do DNA/fisiologia , Diltiazem/farmacologia , Relação Dose-Resposta a Droga , Enalaprilato/farmacologia , Feminino , Metoprolol/farmacologia , Músculo Liso Vascular/fisiologia , Nifedipino/farmacologia , Fator de Crescimento Derivado de Plaquetas/fisiologia , Ratos , Ratos Endogâmicos WKYRESUMO
This paper describes the production and evaluation of an antiserum with high affinity (Ka = 1.9 X 10(11) l/mol) and specifity to 8-arginine-vasopressin (AVP). The antiserum binds only to the intact and unchanged ring and tail of the AVP-molecule. AVP was labelled with 125I by a modification of the chloramin T method and purified by gel-filtration. We describe the development and validation of a radioimmunoassay for AVP in human plasma and urine, using only one extraction method (octa-decasilyl-treated silica microcolumns) for both biological fluids. The overall sensitivity of the assay method is 0.3 pg/ml plasma and 0.6 pg/ml urine. Mean (+/- SD) plasma AVP-concentration in normally hydrated females was 1.2 +/- 0.6 pg/ml (median 1.2 pg/ml) and 1.7 +/- 0.7 pg/ml (median 1.8 pg/ml) in males. Mean urinary AVP excretion was 73 +/- 43 ng/day with ad libitum water intake and normal activity. Sex differences were not statistically significant. We also assessed the response of plasma AVP and urinary AVP-excretion to waterload and dehydration.
Assuntos
Arginina Vasopressina/análise , Radioimunoensaio/métodos , Adulto , Arginina Vasopressina/sangue , Arginina Vasopressina/imunologia , Arginina Vasopressina/urina , Cromatografia em Gel , Reações Cruzadas , Feminino , Humanos , Soros Imunes , Masculino , Pessoa de Meia-Idade , Peptídeos/imunologia , Valores de Referência , Sede , Água/administração & dosagemRESUMO
To investigate the effect of chronic alpha-adrenergic receptor blockade on renin release, plasma renin activity (PRA) was determined overnight at short intervals in 9 patients with essential hypertension before and after 7 days medication with phenoxybenzamine (20 mg orally/day). Inhibition of basal (supine) renin secretion in response to alpha-adrenergic receptor blockade was more apparent in patients with elevated PRA (n = 3) than in those with normal PRA. On the other hand, patients with low PRA (n = 2) even showed an increase in renin release. In addition, night-day variations with secretory episodes in PRA were blunted during drug administration. It is suggested that alpha-adrenergic receptor blockade inhibits renin secretion distal to its blockade of specific adrenergic receptors. However, the increase in renin release during phenoxybenzamine observed in patients with low PRA indicates that the responsiveness of renin secretion to stimulatory effects (most probably induced by the lowered blood pressure in our patients) remained intact during alpha-adrenergic receptor blockade.
Assuntos
Antagonistas Adrenérgicos alfa/farmacologia , Pressão Sanguínea/efeitos dos fármacos , Hipertensão/fisiopatologia , Renina/metabolismo , Adulto , Humanos , Fenoxibenzamina/farmacologia , Taxa Secretória , Fatores de TempoRESUMO
In primary aldosteronism due to an adrenal adenoma (n=2), treatment with a spirolactone (160 mg Canrenone/day for 7 days) decreased plasma aldosterone and urinary aldosterone-18-glucuronide. However, in the presence of a normalization in urinary aldosterone 18-glucuronide plasma aldosterone remained elevated above normal. Continued therapy with higher doses (320 mg/day for 7 days and 480 mg/day for 28 days) did not significantly alter plasma aldosterone, while urinary aldosterone-18-glucuronide returned to values comparable to those obtained before therapy. Cessation of the drug resulted in a marked increase in plasma aldosterone and urinary aldosterone-18-glucuronide. The results indicate that in primary aldosteronism due to an adrenal adenoma, the spirolactone (Canrenone) inhibits aldosterone biosynthesis and seems to influence aldosterone degradation.
Assuntos
Aldosterona/metabolismo , Canrenona , Hiperaldosteronismo/metabolismo , Pregnadienos , Adenoma/complicações , Adenoma/metabolismo , Neoplasias das Glândulas Suprarrenais/complicações , Neoplasias das Glândulas Suprarrenais/metabolismo , Aldosterona/sangue , Aldosterona/urina , Feminino , Glucuronatos/urina , Humanos , Hiperaldosteronismo/etiologia , Cinética , Potássio/sangue , Renina/sangueRESUMO
In patients with essential hypertension a gradual decrease in basal and stimulated renin secretion was found with increasing age. Stimulated plasma aldosterone decreased similarly; however, the observed changes were less pronounced. Young patients (less than 35 years) with high renin hypertension had lower diastolic blood pressure than patients with low renin hypertension in the same age group. Contrary to these findings, a markedly higher diastolic blood pressure was observed in patients over 35 years of age with high renin hypertension than in the group of patients with low renin hypertension. These results indicate that neither high nor low renin essential hypertension patients represent homogeneous groups. Furthermore, the dissociation between changes in renin activity and plasma aldosterone points to a disturbed relationship between the renin angiotensin system and aldosterone secretion in essential hypertension.