[Evaluation of the effect of hemolysis, bilirubin, lipemy, glucosis and natrium on mean concentration of hemoglobin (XN 2000® Sysmex®)]. / Évaluation de l'influence de l'hémolyse, de l'ictère, de la lipémie, d'un apport en glucose ou en sodium sur la concentration corpusculaire moyenne en hémoglobine (XN 2000® Sysmex®).

We evaluated the effect of haemolysis, bilirubin, lipemia, hyperglycemia and natremia on mean corpuscular haemoglobin concentration (MCHC) measured by XN 2000® (Sysmex®). Our evaluation had shown than correction is necessary for haemolysis and lipemia but not for icterus. There were not possible to reproduce variation of MCHC with hyperglycemia or variation of natremia. Blood smears show red blood cells abnormalities.

A novel multiple locus variable number of tandem repeat (VNTR) analysis (MLVA) method for Propionibacterium acnes.

Propionibacterium acnes plays a central role in the pathogenesis of acne and is responsible for severe opportunistic infections. Numerous typing schemes have been developed that allow the identification of phylotypes, but they are often insufficient to differentiate subtypes. To better understand the genetic diversity of this species and to perform epidemiological analyses, high throughput discriminant genotyping techniques are needed. Here we describe the development of a multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) method. Thirteen VNTRs were identified in the genome of P. acnes and were used to genotype a collection of clinical isolates. In addition, publically available sequencing data for 102 genomes were analyzed in silico, providing an MLVA genotype. The clustering of MLVA data was in perfect congruence with whole genome based clustering. Analysis of the clustered regularly interspaced short palindromic repeat (CRISPR) element uncovered new spacers, a supplementary source of genotypic information. The present MLVA13 scheme and associated internet database represents a first line genotyping assay to investigate large number of isolates. Particular strains may then be submitted to full genome sequencing in order to better analyze their pathogenic potential.