RESUMO
We report a draft genome sequence of Nitrosococcus oceani strain NS58, isolated from Tokyo Bay sediment. The genome sequence of strain NS58 was nearly identical (>99.99%) to those of other strains of N. oceani isolated from different ocean regions. Only nine single-nucleotide polymorphisms were identified between N. oceani ATCC 19707T and NS58.
RESUMO
Tetraheme cytochrome c-554 is a physiological electron acceptor of hydroxylamine oxidoreductase (HAO), a core enzyme of ammonia oxidation in chemoautotrophic nitrifiers. Here we report the purification of cytochrome c-554 from Nitrosococcus oceani strain NS58, a marine gammaproteobacterial ammonia-oxidizing bacterium. The NS58 cytochrome is a 25 kDa-protein having four hemes c. The absorption spectrum of the cytochrome showed peaks at 420 nm, 523 nm, and 554 nm, with shoulders at around 430 nm and 580 nm in the reduced state. In contrast to the highly basic counterpart from the betaproteobacterium Nitrosomonas europaea, the NS58 cytochrome c-554 was an acidic protein whose isoelectric point was 4.6. HAO was also purified, and the reaction with the NS58 cytochrome was found to be salt-tolerant. Compared with the activity observed in a non-salt solution, 60% of the activity remained in a saline concentration comparable to that of seawater.
Assuntos
Chromatiaceae/efeitos dos fármacos , Chromatiaceae/enzimologia , Grupo dos Citocromos c/metabolismo , Oxirredutases/metabolismo , Cloreto de Sódio/farmacologia , Sequência de Aminoácidos , Amônia/metabolismo , Proteínas de Bactérias/química , Proteínas de Bactérias/efeitos dos fármacos , Proteínas de Bactérias/isolamento & purificação , Proteínas de Bactérias/metabolismo , Catálise , Chromatiaceae/genética , Chromatiaceae/metabolismo , Grupo dos Citocromos c/efeitos dos fármacos , Grupo dos Citocromos c/genética , Grupo dos Citocromos c/isolamento & purificação , DNA Bacteriano/isolamento & purificação , DNA Ribossômico/química , DNA Ribossômico/genética , Transporte de Elétrons , Heme/química , Cinética , Microscopia Eletrônica de Transmissão , Modelos Moleculares , Dados de Sequência Molecular , Oxirredução , Oxirredutases/efeitos dos fármacos , Oxirredutases/genética , Oxirredutases/isolamento & purificação , Filogenia , RNA Ribossômico 16S/genética , Tolerância ao Sal , Água do Mar/microbiologia , Alinhamento de Sequência , Análise de Sequência de DNA , EspectrofotometriaRESUMO
A 66-year-old man with erysipelas was admitted with complaints of oliguria and massive proteinuria/hematuria. He was diagnosed as having acute poststreptococcal glomerulonephritis(APSGN) due to erysipelas infected by group A streptococcus pyogenes. On admission, his white cell count increased to 31,000, and CRP was 27.3 mg/dl. Serum urea nitrogen and creatinine were increased to 90.1 mg/dl and 4.5 mg/dl, respectively. He had diabetes mellitus(HbA1c 7.9%) and liver dysfunction(total bilirubin 3.5 mg/dl, AST 76 IU, ALT 41 IU) caused by alcoholic liver cirrhosis. Hypocomplementemia was found in addition to ASO 216 U/ml and ASK 10,240 x. After antibiotics treatment was initiated, inflammation of the erysipelas began to improve. Disseminated intravascular coagulation syndrome, probably due to sepsis, occurred on the 5th hospital day. He died of gastrointestinal bleeding on the 18th hospital day. Renal autopsy revealed 37% formation of fibrocellular crescents, and marked mesangiolysis was noted by light microscopy. Granular deposition of C3 and IgG was seen along the capillary walls on immunofluorescence study. Intramembranous deposits were scattered on electron microscopy. This case illustrates a fulminant type of APSGN, which was in part attributed to the presence of diabetes and alcoholic liver cirrhosis. Histological findings of crescent formation and marked mesangiolysis may account for the fulminant clinical course.
