RESUMO
Scopulariopsis brevicaulis, a soil saprophyte, is the most common dermatomycotic mould and causes deep fungal infection. Ten canaries died in a flock of 200 and, at necropsy, S. brevicaulis was isolated from lung and beak samples. Macroscopically, the colonies were flat, velvety or powdery, white, tan, dark brown, grey or black. Microscopically, the isolated fungus had hyaline and septate hyphae, finger-like conidiophores on which annelids produced chains of conidia. On histopathological examination, multiple irregular thin red hyphae were seen in lung tissue of the canaries. Although S. brevicaulis may be involved in onychomycosis, pulmonary mycosis or invasive infection in humans, this infection has not been reported in canaries. This study shows that S. brevicaulis can cause invasive and fatal infection in canaries.
Assuntos
Micoses , Onicomicose , Scopulariopsis , Animais , Humanos , Micoses/microbiologia , Micoses/veterinária , Onicomicose/microbiologia , Onicomicose/patologia , Onicomicose/veterináriaRESUMO
Canine transmissible venereal tumor (CTVT) is a tumor that commonly occurs in genital and extragenital sites of both genders. Long interspersed nuclear elements (LINE-1) retrotransposon has a pivotal role in allogenic transfection among uncontrolled dog populations. This study aimed to perform pathomorphological, immunohistochemical, and in situ polymerase chain reaction (PCR) evaluation of CTVT (n = 18) in transfected dogs during chemotherapy. Immunohistochemically, tumor phases were investigated by using specific markers (CD3, CD4, CD8, CD79, and transforming growth factor beta [TGF-ß]), and investigated an amplified specific sequence of TVT LINE-1 retrotransposon by in situ PCR. Polyhedral-shaped neoplastic cells that had large, round, hypo/hyperchromatic nuclei and eosinophilic cytoplasm were detected. All marker results were positive, especially in the early weeks of recovery. CD4 and TGF-ß markers were conspicuously positive at the initial stage. In situ PCR LINE-1 sequence was initially positive in only four cases. It is believed that the CD and TGF-ß markers provide phase identification at tumor initiation and during chemotherapy. It is thought that presence of T and B lymphocytes, which have roles in cellular and humoral immunity, is needed so that regression of the tumor is possible.
Assuntos
Biomarcadores Tumorais/análise , Doenças do Cão/diagnóstico , Elementos Nucleotídeos Longos e Dispersos , Tumores Venéreos Veterinários/diagnóstico , Animais , Antígenos de Diferenciação de Linfócitos T/análise , Antígenos CD79/análise , Doenças do Cão/metabolismo , Doenças do Cão/terapia , Cães , Feminino , Masculino , Transfecção/veterinária , Tumores Venéreos Veterinários/metabolismo , Tumores Venéreos Veterinários/terapiaRESUMO
Canine transmissible venereal tumor (CTVT) is a tumor that commonly occurs in genital and extragenital sites of both genders. Long interspersed nuclear elements (LINE-1) retrotransposon has a pivotal role in allogenic transfection among uncontrolled dog populations. This study aimed to perform pathomorphological, immunohistochemical, and in situ polymerase chain reaction (PCR) evaluation of CTVT (n = 18) in transfected dogs during chemotherapy. Immunohistochemically, tumor phases were investigated by using specific markers (CD3, CD4, CD8, CD79, and transforming growth factor beta [TGF-β]), and investigated an amplified specific sequence of TVT LINE-1 retrotransposon by in situ PCR. Polyhedral-shaped neoplastic cells that had large, round, hypo/hyperchromatic nuclei and eosinophilic cytoplasm were detected. All marker results were positive, especially in the early weeks of recovery. CD4 and TGF-β markers were conspicuously positive at the initial stage. In situ PCR LINE-1 sequence was initially positive in only four cases. It is believed that the CD and TGF-β markers provide phase identification at tumor initiation and during chemotherapy. It is thought that presence of T and B lymphocytes, which have roles in cellular and humoral immunity, is needed so that regression of the tumor is possible.
Assuntos
Animais , Cães , Linfócitos B , Citoplasma , Tratamento Farmacológico , Eosinófilos , Imunidade Humoral , Imuno-Histoquímica , Reação em Cadeia da Polimerase , Retroelementos , Transfecção , Fator de Crescimento Transformador beta , Tumores Venéreos VeterináriosRESUMO
Axonal damage has been proposed as the major substrate of permanent clinical disability in multiple sclerosis. Tau protein, a microtubule-associated protein localised in neuronal axons, may serve as a biochemical surrogate marker to evaluate axonal damage in vivo. We intended to determine the extent of axonal damage in different stages and clinical subtypes of MS by investigating cerebrospinal fluid tau concentrations. Tau was measured using an immunoassay in 35 patients with relapsing-remitting MS, eight patients with secondary progressive MS, nine patients with primary progressive MS, 50 patients with clinically isolated syndrome suggestive of early MS and 46 normal controls. Cerebrospinal fluid tau was significantly elevated in MS compared with normal controls (median 206.0 pg/mL versus 152.0 pg/mL; P = 0.002). No significant difference among different subtypes of MS could be detected, although highest levels were found in very early disease stages. There was a significant elevation of CSF tau among patients with gadolinium-enhancing brain lesions in magnetic resonance imaging (P = 0.02) and a tendency towards higher CSF tau levels in patients with pronounced intrathecal IgG synthesis, supporting the notion that axonal damage is influenced by inflammatory activity.
Assuntos
Esclerose Múltipla Crônica Progressiva/líquido cefalorraquidiano , Esclerose Múltipla Crônica Progressiva/diagnóstico , Esclerose Múltipla Recidivante-Remitente/líquido cefalorraquidiano , Esclerose Múltipla Recidivante-Remitente/diagnóstico , Proteínas tau/líquido cefalorraquidiano , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/líquido cefalorraquidiano , Diagnóstico Precoce , Feminino , Humanos , Imunoglobulina G/líquido cefalorraquidiano , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Esclerose Múltipla Crônica Progressiva/epidemiologia , Esclerose Múltipla Recidivante-Remitente/epidemiologia , Prognóstico , Fatores de Risco , Índice de Gravidade de DoençaRESUMO
H-type tracheoesophageal fistula is a rare congenital condition. Its rarity and concurrent problems, such as respiratory distress, may delay its detection. We report delay in the diagnosis in a preterm small-for-gestational-age baby who showed symptoms of apnea and recurrent pneumonia, even when she was being fed by orogastric tube.