The Effect of Grape Seed Extract on the Alveolar, Jaw, and Skeletal Bone Remodeling: A Scoping Review.

Herbal medicine has an important part in promoting and maintaining human health. One of them was grape seed extract (GSE). Various potentials of GSE in human health have been explored, and its potential for maintaining bone health is promising. Some initial research has provided evidence that the GSE was able to affect bone remodeling (bone resorption and bone formation). This scoping review analyzed and discussed all the reports on the effect of GSE on bone healing and bone remodeling in animals in the alveolar bone, jaw bone, and skeletal bone. The further purpose is to give an opportunity to research and development of supplementation of GSE for humans.The Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) 2020 guidelines were used to compose this scoping review through database on Scopus, PubMed, Science Direct, Web of Science, Embase, and manual search until December 2022. The inclusion criteria were a study that analyzed the effect of supplementation GSE on all bones.All included study was in vivo study with supplementation of GSE. The supplementation of GSE affects the alveolar bone, jaw bones, and skeletal bone by promoting bone formation and inhibiting bone resorption by suppressing inflammation, apoptosis pathways, and osteoclastogenesis. It not only supports bone remodeling in bone inflammation, osteonecrosis, osteoporosis, and arthritis but also the GSE increases bone health by increasing the density and mineral deposition in trabecula and cortical bone.The supplementation of GSE supports bone remodeling by interfering with the inflammation process and bone formation not only by preventing bone resorption but also by maintaining bone density.

Gingiva Mesenchymal Stem Cells Normoxic or Hypoxic Preconditioned Application Under Orthodontic Mechanical Force on Osterix, Osteopontin, and ALP Expression.

OBJECTIVES: The aim of this article was to investigate Osterix, ALP, and osteopontin expression in the compression and tension sides of alveolar bone after the application of normoxic/hypoxic-preconditioned GMSCs in rabbits (Oryctolagus cuniculus) induced with OMF. MATERIALS AND METHODS: Forty-eight healthy, young male rabbits were divided into four groups: [-] OMF; [+] OMF; OMF with GMSCs normoxic-preconditioned; and OMF and GMSCs hypoxic-preconditioned. The central incisor and left mandibular molar in the experimental animals were moved, the mandibular first molar was moved mesially using nickel titanium (NiTi) and stainless steel ligature wire connected to a 50 g/mm2 light force closed coil spring. Allogeneic application of normoxic or hypoxic-preconditioned GMSCs was used in as many as 106 cells in a 20 µL phosphate buffered saline single dose and injected into experimental animals' gingiva after 1 day of OTM. On days 7, 14, and 28, all experimental animals were euthanized. Osterix, ALP, and osteopontin expressions were examined by immunohistochemistry. RESULTS: Osterix, ALP, and osteopontin expressions were significantly different after allogeneic application of hypoxic-preconditioned GMSCs than normoxic-preconditioned GMSCs in the tension and compression of the alveolar bone side during OMF (p < 0.05). CONCLUSION: Osterix, ALP, and osteopontin expressions were significantly more enhanced post-transplantation of GMSCs with hypoxic-preconditioning than after transplantation of normoxic-preconditioned GMSCs in rabbits (O. cuniculus) induced with OMF.

COL1A1 and FGFR2 Single-Nucleotide Polymorphisms Found in Class II and Class III Skeletal Malocclusions in Javanese Population.

OBJECTIVE: The aim of this article is to analyze and compare the presence of single-nucleotide polymorphisms (SNPs) of COL1A1 and FGFR2 in class II and class III Javanese populations. MATERIALS AND METHODS: Cephalometric radiographs from total 63 patients of class II and III were analyzed. SNP analysis was performed based on both COL1A1 and FGFR2 sequences amplified from total DNA of patients' fresh blood. Principal component analysis was done to calculate the data and find the correlation of the cephalometric indicators influenced by each mutation. t-test and Mann-Whitney analysis were performed to check the significance of differences occurred in each studied parameter (p < 0.05). RESULT: There were three COL1A1 SNPs found in class II and only two in class III, while three FGFR2 SNPs found in both classes. Class II phenotype seemed to be strongly influenced by Y-axis and mandibular plane angle, while class III by lower gonial angle and mandibular plane angle. CONCLUSION: Based on this study, we suggest that rs2249492 of COL1A1 and rs2981582 of FGFR2 play important roles in class III, while rs2277632 of COL1A1 and rs2981582 of FGFR2 play important role in class II skeletal malocclusion in Javanese population.

Anti-Peri-implantitis Bacteria's Ability of Robusta Green Coffee Bean (Coffea Canephora) Ethanol Extract: An In Silico and In Vitro Study.

OBJECTIVE: This study was aimed to investigate RGCBE extract as antioxidant and anti-peri-implantitis bacteria through in vitro study and its potential as antioxidant, antibacterial, anti-inflammatory, antibone resorption, and proosteogenic through in silico study. MATERIALS: AND METHODS: Absorption, distribution, metabolism, excretion and toxicity prediction, molecular docking simulation, and visualization of chlorogenic acid (CGA) and coumaric acid (CA) as anti-inflammatory, antioxidant, and antibacterial were investigated in silico. Inhibition zone by diffusion method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of RGCBE extract against Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), and Prevotella intermedia (Pi) were done. STATISTICAL ANALYSIS: the analysis of variance (ANOVA) difference test, and the post-hoc Tukey's Honest Significant Different (HSD) with a different significance value of p<0.05 RESULTS: GCA and CA compounds are good drug molecules and it has low toxicity. Chlorogenic acid have higher binding activity than coumaric acid to tumor necrosis factor (TNF)-α, nuclear factor (NF)-κB, receptor activation NF-κB (RANK) and its ligand (RANKL), interleukin (IL)-6, IL-10, runt related transcription factor (RUNX2), receptor activator nuclear Kappa beta Ligand-osteoprotegrin osteocalcin (RANKL-OPG), osteocalcin, nuclear factor associated T-cell 1 (NFATc1), tartate resistant acid phosphatase (TRAP), peptidoglycan, flagellin, dectin, Hsp70, and Hsp10 protein. RGCB ethanol extract has high antioxidant ability and it has MIC, MBC, and inhibit the growth of Aa, Pg, Fn, and Pi at 50% concentration with significantly different (p=0.0001 and<0.05). CONCLUSION: RGCB ethanol extract has high antioxidant ability and 50% RGCB ethanol extract may act as strong anti-peri-implantitis bacteria in vitro. In addition, CGA in RGCB potential as antioxidant, antibacterial, anti-inflammatory, antibone resorption, and proosteogenic in silico.

Effect of Caffeic Acid Phenethyl Ester Provision on Fibroblast Growth Factor-2, Matrix Metalloproteinase-9 Expression, Osteoclast and Osteoblast Numbers during Experimental Tooth Movement in Wistar Rats (Rattus norvegicus).

OBJECTIVES: To investigate the effect of caffeic acid phenethyl ester (CAPE) provision on matrix metalloproteinase-9 (MMP-9), fibroblast growth factor-2 (FGF-2) expression, osteoclast and osteoblast numbers during experimental orthodontic tooth movement (OTM) in male Wistar rats (Rattus norvegicus). MATERIALS AND METHODS: Forty-eight healthy male Wistar rats (R. norvegicus), 16 to 20 weeks old with 200 to 250 g body weight (bw) were divided into several groups as follows: K1: OTM for 3 days; K2: OTM for 7 days; K3: OTM for 14 days; KP1: OTM and CAPE for 3 days; KP2: OTM and CAPE for 7 days; and KP3: OTM and CAPE for 14 days. A nickel titanium closed coil spring 8.0 mm long with 10 g/mm2 was installed between the upper left first molar and upper central incisor to move molar mesially. CAPE provision with a dose of 20 mg/kg bw of animal studies was done per orally. Immunohistochemistry was done to examine MMP-9 expression and osteoclast number in compression side as well as FGF-2 expression and osteoblast number in tensile side of the OTM. STATISTICAL ANALYSIS: One-way analysis of variance test and Tukey's honest significant difference test were performed to determine the difference between the groups (p < 0.05). RESULTS: MMP-9 expression and osteoclast numbers in the compression side were significantly different between the groups. Similarly, FGF-2 expression and osteoclast numbers in the tensile side were significantly different between the groups. CONCLUSIONS: CAPE provision during OTM increases the number of osteoblasts and the FGF-2 expression significantly in the tensile side. Osteoclast numbers and MMP-9 expression significantly decrease in the compression side.

Correlation between Dentoalveolar Heights and Vertical Skeletal Patterns in Class I Malocclusion in Ethnic Javanese.

OBJECTIVES: Vertical proportions of the face are important determining factors for diagnosis and planning appropriate orthodontic treatment. Orthodontic patients have different vertical and sagittal skeletal discrepancies, as well as associated varying degrees of dentoalveolar compensations. Dentoalveolar is a functional component of the jaw; it plays a role in occlusal dynamics and forms sagittal and vertical maxilla-mandibula relationships. This study aims to analyze the relationship between dentoalveolar heights and several vertical skeletal patterns in patients with Class I malocclusion in ethnic Javanese. MATERIALS AND METHODS: The sample consisted of lateral cephalograms of 75 patients (18 samples were male, and 57 were female). Determined by inclusion and exclusion criteria, the participants were selected from an initial sample of 196 patients with skeletal Class I malocclusion (sella-nasion-A and B [ANB] = 1-4 degrees). Cephalometric analysis was performed using OrthoVision2017 digital software. This analysis measured upper anterior dental height (UADH), upper posterior dental height (UPDH), lower anterior dental height (LADH), lower posterior dental height (LPDH), ANB angle, sella-nasion and mandibular plane (SN-MP), sella-nasion and palatal plane (SN-PP), palatal plane and mandibular plane (PP-MP), Frankfort horizontal plane and mandibular plane (FH-MP), sella to gonion (S-Go), articulare to gonion (Ar-Go), nasion to menton (N-Me), nasion to anterior nasal spine (N-ANS), and anterior nasal spine to menton (ANS-Me). Pearson correlation test was used to assess correlations among all variables (p < 0.05). RESULTS: Significant correlations were observed between dentoalveolar heights and SN-MP, S-Go, Ar-Go, N-Me, and ANS-Me (p < 0.05). CONCLUSIONS: Patients with Class I malocclusion in ethnic Javanese exhibit a significant correlation between dentoalveolar and vertical skeletal patterns. UPDH and/or LPDH have a significantly positive correlation with SN-MP, S-Go, Ar-Go, N-Me, and ANS-Me. The orthodontic correction of the decreased or increased facial height included either the extrusion or intrusion of the anterior or posterior teeth in different ways.

Gonial Angle Characteristics of Class III Malocclusion in Javanese Ethnic

ABSTRACT Objective: To describe the gonial angle characteristics in class III skeletal malocclusion in Javanese ethnic. Material and Methods: Pretreatment lateral cephalometric radiographs of 43 Javanese ethnic patients were measured: upper (Go1) and lower gonial angle (Go2), anterior (AFH) and posterior face height ratio (PFH), maxilla-mandibular length difference, mandibular plane angle (FMA), Y axis, ramus position, ANB angle, posterior cranial base/ramus height and mandibular body length/anterior cranial base. The relation between Go1, Go2 and other variables were analysed using correlation and regression analysis. Results: The total gonial angle is within normal range, but Go1 is below normal and Go2 is above normal. There is no difference between male and female gonial angle measurements (p=0.939 and p=0.861, respectively). Ramus position is positively correlated to Go1 (p=0.003), while AFH (p=0.000), maxilla-mandibular length difference (p=0.000), FMA (p=0.000), Y axis (p=0.000), and posterior cranial base/ramus height (p=0.018) are positively correlated to Go2. PFH is negatively correlated to Go2 (p=0.018). Conclusion: The upper gonial angle is influenced by the position of mandibular ramus, while the lower gonial angle is affected by the posterior and anterior lower facial height and mandibular size and rotation. Javanese with class III malocclusion tends to have hypodivergent facial type, with more posteriorly located mandibular ramus and excess mandibular length.

The Importance of the Occlusal Plane in Predicting Better Facial Soft Tissue in Class II Malocclusion in Ethnic Javanese.

OBJECTIVES: Class II malocclusion is largely due to a retrognathic mandible. Mandibular rotation is closely related to changes in the occlusal plane during growth. The problems in the occlusal plane could cause disadvantages in the soft tissue profile in Class II malocclusion, presenting treatment challenges for an orthodontist. This study aimed to investigate the importance of the occlusal plane for a better soft tissue profile in Class II malocclusion for Javanese patients. MATERIALS AND METHODS: The total number of cephalogram softcopies of patients with skeletal Class II malocclusion were selected based on ANB values (> 4 degrees), no agenesis teeth except the third molar, and all permanent teeth. The cephalograms were calculated using digital tracing by Morpheus 3D imaging. The points and areas to be analyzed on the cephalogram were predetermined. The examination was performed in a span of 1 month and performed via a statistical test using Pearson's test and multiple regression analysis (p < 0.05). RESULTS: There were significant correlation values between the angles produced by the occlusal plane to sella national, Frankfurt horizontal, mandibular plane, and Z-angle (p < 0.05). CONCLUSION: Patients with skeletal Class II malocclusion have a significant correlation between the occlusal plane and the vertical plane, thereby affecting the shape of the soft tissue profile, which causes a facial imbalance. By improving mandibular movement, the soft tissue profile can also be corrected.

The Occlusal Plane Inclination Analysis for Determining Skeletal Class III Malocclusion Diagnosis.

INTRODUCTION: The occlusal plane is very important in the stomatognathic and aesthetic dentofacial system. Manipulation of the occlusal plane plays an important role in class III malocclusion correction. For this reason, the strategy in establishing the diagnosis, prognosis and treatment plan becomes more complex, so a more comprehensive analysis of the occlusal plane inclination as a parameter of diagnosis is needed for more effective and efficient treatments. AIM: The aim of this study was to determine the comparison of occlusal plane inclination between class III and class I malocclusions and to knowing the correlation of occlusal plane inclination in class III malocclusions with the direction of facial growth. MATERIALS AND METHODS: Samples were secondary lateral cephalograms collected at the Universitas Airlangga (UNAIR) Dental Hospital in October 2017-2018. Cephalograms were traced using the OrthovisionTM software; then, the Mann-Whitney comparative statistical test (p<0.05) was carried out followed by the canonical correlation test. RESULTS: Differences in class III and class I malocclusions were found in the OP-AB and OP-FH angles. The OP-AB angle is the occlusal plane inclination which has the strongest correlation compared to others. OP-AB angle is strongly correlated with horizontal growth parameter, especially theSNB. The OP-AB angle is also correlated with vertical growth parameters: the Y axis and the Facial axis. CONCLUSION: The OP-AB angle is a parameter that can distinguish between class III and class I malocclusions. The OP-AB angle has a strong correlation with vertical and horizontal growth directions.

Single Nucleotide Polymorphisms (SNPs) of COL1A1 and COL11A1 in Class II Skeletal Malocclusion of Ethnic Javanese Patient.

BACKGROUND: The prevalence of malocclusion cases in the orthodontic specialist clinic in Airlangga University's Dental Hospital in Surabaya, Indonesia, in 2014-2016 is fairly high, as 55.34% of the occurrences were identified as class II skeletal malocclusion. This type of skeletal malocclusion, which is usually recognized in adults, occurs as a result of variation during growth and development. Lately, there have been many reports on gene polymorphisms of COL1A1 and COL11A1, which are assumed to be associated with class II skeletal malocclusion in Caucasians. PURPOSE: This study aims to analyze the relationship between single nucleotide polymorphisms (SNPs) of COL1A1 and COL11A1 with class II skeletal malocclusion in Javanese ethnic group patients with mandibular micrognathism. MATERIALS AND METHODS: The diagnosis of class II skeletal malocclusion was established using the lateral cephalometric radiographs (ANB angle ≥4°) (n=50). DNA was extracted from the patient's peripheral blood. After that, PCR, electrophoresis, and DNA sequencing were conducted on the extracted DNA based on COL1A1 and COL11A1 primers. RESULTS: The SNPs in COL1A1 are c.20980G/A in 27 patients and c.20980G>A in 8 patients, whereas SNPs in COL11A1 are both c.134373C/A and c.134555C/T in 8 patients and both c.[134373A>C] and c.134582G>A in 10 patients. All samples show the deletion (c.[134227delA]) in COL11A1. CONCLUSION: SNPs in COL1A1 and COL11A1 have been found in class II skeletal malocclusion of Javanese ethnic group patients. Seventy percent of SNPs in COL1A1 occur in rs.2249492, whereas 36% of newly discovered SNPs appear in COL11A1. All samples also have deletion in COL11A1.

High Mobility Group Box 1 and Heat Shock Protein-70 Expression Post (-)-Epigallocatechin-3-Gallate in East Java Green Tea Methanolic Extract Administration During Orthodontic Tooth Movement in Wistar Rats

Abstract Objective: To investigate the expression of High Mobility Group Box 1 (HMGB1) and Heat Shock Protein-70 (HSP-70) during orthodontic tooth movement (OTM) after (-)- Epigallocatechin-3-Gallate (EGCG) in East Java Green Tea (Camelia Sinensis) Methanolic Extract (GTME) administration in vivo. Material and Methods: 28 Wistar rats (Rattus Novergicus) was used and divided into 4 groups accordingly: K- without EGCG and OTM; K+ with OTM, without EGCG for 14 days; T1with OTM for 14 days and EGCG for 7 days; treatment group 2 (T2) with OTM and EGCG for 14 days. OTM animal model was achieved through the installation of the OTM device by means of NiTi close coil spring with 10g force placed between the first incisor and first maxillary molars. The samples were terminated on Day 14. The pre-maxillary was isolated for the immunohistochemical examination. Analysis of Variance (ANOVA) then continued with Tukey Honest Significant Difference (HSD) (p<0.05) was performed to analyze the data. Results: The highest HMGB1 and HSP-70 expression were found in the K+ group pressure side, meanwhile the lowest HMGB1 and HSP-70 expression were found in K- group tension side in the alveolar bone. There was a significant decrease of HMGB1 and HSP-70 expression in T2 compared to T1 and K+ with significant between groups (p<0.05; p=0.0001). Conclusion: The decreased expression of HMGB1 and HSP-70 in alveolar bone of OTM wistar rats due to post administration of GTME that consisted EGCG.

The effect of miniscrew length and bone density on anchorage resistance: An in vitro study.

INTRODUCTION: With conventional anchorage, it is usually hard to accomplish a satisfactory result with an absolute anchorage, and this limitation could be resolved by the usage of mini-screw. The successful rate of miniscrew usage depends on its stability, which was determined by its length, bone density, cortical bone thickness, the insertion technique, the insertion angle, and the applied loads. OBJECTIVE: To observe the effect of miniscrew length and bone density on anchorage resistance. MATERIAL AND METHODS: Thirty pieces of miniscrew with 1.6mm in diameter were divided into three groups based on its length (n=10): 10mm (L), 8mm (M), and 6mm (S). Each group was further divided into 2 sub-groups: to be planted in optimal density bovine ribs (L1, M1, S1) and to be planted in low-density bovine ribs (L2, M2, S2). The density of bovine ribs was measured by CBCT. After the insertion of miniscrews based on respective groups, tensile test was done by means of Autograf Univerval Testing Machine to measure its stability. The data recorded was analysed using the Least Significant Difference (LSD) Fisher's test. RESULTS: The results of this study showed that L1 provided the greatest stability than other groups. On the other hand, the least stability was found in S2. CONCLUSION: The length of miniscrew and the density of bone affect the stability of miniscrew. In bone with optimum density, 10mm and 8mm miniscrew equipped good anchorage resistance while in bone with low-density only 10mm miniscrew provided favourable anchorage resistance.

Correlation between vertical components and skeletal Class II malocclusion in ethnic Javanese.

INTRODUCTION: The dentoskeletal morphology of Class II malocclusion has been analyzed in several cephalometric investigations. It is crucially important to understand the vertical components in orthodontic treatment. Defining the facial type of an individual is an essential key to obtain an accurate orthodontic diagnosis. AIM: The aim of this study was to understand the correlation of vertical components in Class II skeletal malocclusion. MATERIALS AND METHODS: This is a observational descriptive study. This study used lateral cephalograms of Class II skeletal malocclusion from Universitas Airlangga Dental Hospital, between April 2015 and 2016. Cephalometric analysis was performed using digital software by a single examiner. This analysis measured gonial (Go), upper gonial (Go1), lower gonial (Go2), Yaxis-SN angle, the length of anterior face height, and posterior face height. The correlation between each variable was analyzed using the Pearson correlation test (P<0.01). RESULTS: There was a correlation between vertical components and Class II skeletal malocclusion. CONCLUSION: Vertical components were correlated with Class II skeletal malocclusion. The greater ANB angle will be followed by greater Go2, which worsens the condition of Class II skeletal malocclusion.

Force degradation trend of latex and nonlatex orthodontic elastics after 48 hours stretching.

BACKGROUND: Two types of orthodontic elastics exist based on their material, latex and nonlatex, each of which has different properties in clinical use. Some of the differences include their initial force and force degradation over time. This study was conducted to compare the force changes in both materials. AIM: To evaluate the force degradation of latex and nonlatex elastics under moderate stretching over time. METHODS: Medium-force orthodontic latex and nonlatex elastics from American Orthodontics (AO) and Ortho Technology (OT) of lumen size 1/4 inches and 3/16 inches (total sample 110 elastics) were submerged in artificial saliva (pH 6.7) and incubated for 48 hours. Then, the elastic force was measured at the following time intervals: initial, 1, 3, 6, 12, 24, and 48 hours. Orthodontic latex and nonlatex elastics from AO and OT were analyzed using Fourier-Transform Infrared Spectroscopy and energy-dispersive X-ray to know the chemical bond structure and elements. RESULTS: There was a statistically significant difference between latex and nonlatex force degradation over a period of 0-24 hours (P<0.05), while no significant difference existed between 24-48 hours (P>0.05). Force degradation of latex elastics was higher than that of nonlatex elastics. Energy-dispersive X-ray results on nonlatex elastic bands from OT and AO showed higher C element in the latex elastic band from OT, while the latex elastic band from AO had higher Al element. CONCLUSION: Medium-force latex and nonlatex elastics 1/4 inches and 3/16 inches in size both showed force degradation at 1, 3, 6, 12, and 24-hour intervals under 30 mm stretching when kept in artificial saliva (pH 6.7) and incubated at 37°C for 48 hours.

Cephalometric Characteristic of Skeletal Class II Malocclusion in Javanese Population at Universitas Airlangga Dental Hospital.

INTRODUCTION: To describe the cephalometric characteristic of skeletal Class II malocclusion in Javanese Population at Universitas Airlangga Dental Hospital. METHODS: A total of 118 lateral cephalograms of preorthodontic patients with skeletal Class II malocclusion were obtained from Universitas Airlangga Dental Hospital. The lateral cephalograms were analyzed using digital cephalometric analysis to determine the ANB, mandibular length, facial axis, Y-axis, sella to nasion-mandibular plane (SN-MP), and lower anterior facial height (LAFH). Correlation between mandibular length and other variables was analyzed using Pearson correlation test with P < 0.05. RESULTS: There was an increase of ANB, Y-axis, SN-MP, and LAFH. While SNB was decrease and mandibular length was shortened. There was a significant correlation between mandibular length and other variables, such as facial axis, SN-MP, LAFH, and ANB. CONCLUSIONS: Skeletal Class II malocclusion in Javanese Population at Universitas Airlangga Dental Hospital was characterized by short mandibular length and large ANB mostly not by the increased of SNA but by the lack of SNB. The length of mandible correlated with facial axis angle, lower anterior facial height, and mandibular plane angle.