Assuntos
Bactérias/isolamento & purificação , Biodiversidade , Filogenia , Microbiologia do Solo , Arthrobacter/genética , Arthrobacter/isolamento & purificação , Bactérias/classificação , Bactérias/genética , Bradyrhizobium/genética , Bradyrhizobium/isolamento & purificação , Ecossistema , Microscopia Eletrônica , Dados de Sequência Molecular , RNA Ribossômico 16S , SibériaRESUMO
A halotolerant bacterium, strain SMB34T, was isolated from a naphthalene-utilizing bacterial consortium obtained from primitive technogeneous soil (Vrkhnekamsk salt deposit, Perm region, Russia) by enrichment procedure. The strain itself was unable to degrade naphthalene and grew at NaCl concentrations up to 11% (w/v). The 16S rRNA-based phylogenetic analysis showed that the strain belongs to the genus Thalassospira. The DNA-DNA hybridization values between SMB34T and the type strains of phylogenetically closest species (T. xiamenensis, T. profundimaris and T. tepidiphila) did not exceed 50%. The novel strain could be distinguished from the above species by the cell motility, MALDI/TOF mass spectra of whole cells and a range of physiological and biochemical characteristics. SMB34T also considerably differs from the recently described species T. xianhensis, with the most striking differences in the DNA G + C content (53.7 +/- 1.0 vs. 61.2 +/- 1.0 mol.%) and predominant ubiquinones (Q-10 vs. Q-9). The data obtained suggest strain SMB34T (=VKM B-2527T = NBRC 106175T), designated as the type strain, represents a novel species, named Thalassospira permensis sp. nov.
Assuntos
Consórcios Microbianos , Naftalenos/metabolismo , RNA Ribossômico 16S/genética , Rhodospirillaceae/classificação , Rhodospirillaceae/metabolismo , Microbiologia do Solo , Composição de Bases , Filogenia , Rhodospirillaceae/genética , Federação Russa , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodosAssuntos
Caulobacter , Enzimas de Restrição do DNA/metabolismo , DNA Bacteriano/análise , Água Doce/microbiologia , Bactérias Gram-Negativas , RNA Ribossômico 16S/análise , Microbiologia do Solo , Ásia , Técnicas de Tipagem Bacteriana , Caulobacter/classificação , Caulobacter/genética , Caulobacter/isolamento & purificação , Polaridade Celular , Enzimas de Restrição do DNA/genética , DNA Bacteriano/química , Ecossistema , Microbiologia Ambiental , Europa (Continente) , Variação Genética , Bactérias Gram-Negativas/classificação , Bactérias Gram-Negativas/genética , Bactérias Gram-Negativas/isolamento & purificação , Filogenia , Mapeamento por Restrição , Análise de Sequência de DNA , Solo/análiseRESUMO
We studied metabolites synthesized by Bacillus subtilis strains, including the type strain of B. atrophaeus and phenotypically similar cultures. These metabolites were presented by polyene antibiotics with conjugated double bonds. Hexaenes from study strains inhibited the growth of phytopathogenic fungi Fusarium culmorum, F. sporotrichiella, F. oxysporum, Botrytis sorokiniana, Alternaria tenuis, and Phytophtora infestans. The degree of growth inhibition depended on the test fungus.
Assuntos
Antibacterianos/biossíntese , Bacillaceae/metabolismo , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Antifúngicos/farmacologia , Bacillaceae/crescimento & desenvolvimento , Bacillus subtilis/crescimento & desenvolvimento , Bacillus subtilis/metabolismo , Meios de Cultura/metabolismo , Fungos Mitospóricos/efeitos dos fármacos , Fungos Mitospóricos/crescimento & desenvolvimento , Phytophthora/efeitos dos fármacos , Phytophthora/crescimento & desenvolvimento , Saccharomyces cerevisiae/efeitos dos fármacos , Especificidade da EspécieRESUMO
Bacteria belonging to different taxonomic and physiological groups (members of the genera Pseudomonas, Brevibacterium, Rhodopseudomonas, and Lactococcus) are able to form intracellular cobalt- and chromium-containing magnetic inclusions. The paper deals with the structure and the intracellular localization of these inclusions and their similarity to the known noncrystalline iron-containing magnetic inclusions. The possible biological role of the magnetic inclusions is discussed.
Assuntos
Bacilos e Cocos Aeróbios Gram-Negativos/metabolismo , Bactérias Gram-Positivas/metabolismo , Corpos de Inclusão/metabolismo , Cromo/química , Cromo/metabolismo , Cobalto/química , Cobalto/metabolismo , Bacilos e Cocos Aeróbios Gram-Negativos/ultraestrutura , Bactérias Gram-Positivas/ultraestrutura , Corpos de Inclusão/química , Corpos de Inclusão/ultraestrutura , Magnetismo , Microscopia EletrônicaRESUMO
Prokaryotic cells may contain one of two types of magnetic intracellular structures, either crystalline magnetosomes or noncrystalline magnetic inclusions. In a magnetic field, the locomotor behavior of cells containing magnetosomes is categorized as magnetotaxis, whereas noncrystalline magnetic inclusions cause a passive attraction of cells containing such inclusions to a magnet. The review considers the distribution, structure, and function of both types of magnetic particles in prokaryotic cells.
Assuntos
Bactérias/metabolismo , Corpos de Inclusão/metabolismo , Bactérias/classificação , Bactérias/isolamento & purificação , Fenômenos Fisiológicos Bacterianos , Ecologia , Microbiologia Ambiental , Compostos Férricos/análise , Corpos de Inclusão/química , Magnetismo , MovimentoRESUMO
Six strains containing site-specific endonucleases II were selected from a collection of 45 ice-nucleating bacterial strains isolated from rhizosphere of plants growing in various geographical regions. Endonucleases Pfl211I, Psp8I, and Psp23I were isolated and purified from two Pseudomonas sp. strains and a Pseudomonas fluorescens strain. Restriction endonucleases Pfl21I and Psp23I were shown to recognize and cleave the DNA nucleotide sequence 5'-CTGCA decrease G-3'. Endonuclease Psp8I recognized and cleaved the DNA nucleotide sequence 5'-G decrease GATCC-3'. These endonucleases were found to be true isoschizomers of PstI and BamHI, respectively.