RESUMO
A freshwater Se guideline was developed for consideration based on concentrations in fish eggs or ovaries, with a focus on Canadian species, following the Canadian Council of Ministers of the Environment protocol for developing guideline values. When sufficient toxicity data are available, the protocol recommends deriving guidelines as the 5th percentile of the species sensitivity distribution (SSD). When toxicity data are limited, the protocol recommends a lowest value approach, where the lowest toxicity threshold is divided by a safety factor (e.g., 10). On the basis of a comprehensive review of the current literature and an assessment of the data therein, there are sufficient egg and ovary Se data available for freshwater fish to develop an SSD. For most fish species, Se EC10 values (10% effect concentrations) could be derived, but for some species, only no-observed-effect concentrations and/or lowest-observed-effect concentrations could be identified. The 5th percentile egg and ovary Se concentrations from the SSD were consistently 20 µg/g dry weight (dw) for the best-fitting distributions. In contrast, the lowest value approach using a safety factor of 10 would result in a Se egg and ovary guideline of 2 µg/g dw, which is unrealistically conservative, as this falls within the range of egg and ovary Se concentrations in laboratory control fish and fish collected from reference sites. An egg and ovary Se guideline of 20 µg/g dw should be considered a conservative, broadly applicable guideline, as no species mean toxicity thresholds lower than this value have been identified to date. When concentrations exceed this guideline, site-specific studies with local fish species, conducted using a risk-based approach, may result in higher egg and ovary Se toxicity thresholds.
Assuntos
Monitoramento Ambiental , Peixes/metabolismo , Ovário/efeitos dos fármacos , Óvulo/efeitos dos fármacos , Selênio/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Canadá , Feminino , Peixes/crescimento & desenvolvimento , Masculino , Ovário/química , Óvulo/química , Óvulo/crescimento & desenvolvimento , Medição de Risco , Selênio/análise , Especificidade da Espécie , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/normas , Poluição Química da Água/análiseRESUMO
Salt-impacted soils resulting from oilfield brine spills are increasingly becoming a significant problem in oil-producing areas of Canada such as Alberta and Saskatchewan. The native halophyte Atriplex patula is being considered a potential species for phytoremediation of brine-impacted sites in these hemiboreal climactic zones. The objective of this study was to investigate the optimal seeding conditions under field conditions (with no irrigation) of A. patula for phytoremediation of salt from a brine-impacted site. Atriplex patula was identified in preliminary greenhouse trials to have one of the highest salt accumulations in relation to plant yields. Different seeding methods of A. patula were assessed in an attempt to achieve reproducible growth of this species. While plant yields for A. patula were improved on compacted soil by approximately 30-50%, growth was uneven with regard to density and height. The uneven growth may be due to seed quality and low precipitation during the field season, while improvements in plant yield on compact soil might be due to a lack of competition with other species.
Assuntos
Atriplex/crescimento & desenvolvimento , Plantas Tolerantes a Sal/crescimento & desenvolvimento , Alberta , Atriplex/fisiologia , Biodegradação Ambiental , Biomassa , Hordeum/crescimento & desenvolvimento , Salinidade , Plantas Tolerantes a Sal/fisiologia , Sais , Estações do Ano , Sementes/crescimento & desenvolvimento , Solo/análise , Poluentes do Solo , Água/fisiologiaRESUMO
Naphthenic acids (NAs) are a complex mixture of organic acid compounds released during the extraction of crude oil from oil sands operations. The accumulation of toxic NAs in tailings pond water (TPW) is of significant environmental concern, and phytoremediation using constructed wetlands is one remediation option being assessed. Since root-associated microorganisms are an important factor during phytoremediation of organic compounds, this study investigated the impact of NAs on the microbial communities associated with the macrophyte Typha latifolia (cattail). Denaturing gradient gel electrophoresis revealed that the impact of NAs on microbial communities was niche dependent, with endophytic communities being the most stable and bulk water communities being the least stable. The type of NA used was significant to microbial response, with commercial NAs causing greater adverse changes than TPW NAs. In general, plant beneficial bacteria such as diazotrophs were favoured in cattails grown in TPW NAs, while potentially deleterious bacteria such as denitrifying Dechlorospirillum species increased in commercial NA treatments. These findings suggest that NAs may affect plant health by impacting root-associated microbial communities. A better understanding of these impacts may allow researchers to optimize those microbial communities that support plant health, and thus further optimize wetland treatment systems.
Assuntos
Ácidos Carboxílicos/farmacologia , Raízes de Plantas/microbiologia , Typhaceae/microbiologia , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Sequência de Bases , Primers do DNA , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , DNA de Plantas/genética , DNA de Plantas/isolamento & purificação , Hidroponia/métodos , Dados de Sequência Molecular , Filogenia , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , RNA Bacteriano/genética , RNA Bacteriano/isolamento & purificação , RNA de Plantas/genética , RNA Ribossômico 16S/genética , Typhaceae/efeitos dos fármacos , Typhaceae/genética , Typhaceae/crescimento & desenvolvimentoRESUMO
There is interest in using mature fine tailings (MFT) in reclamation strategies of oil sands mining operations. However, simulated runoff from different dried MFT treatments is known to have elevated levels of salts, toxic ions, and naphthenic acids, and alkaline pH and it is phytotoxic to the emergent macrophyte, common reed (Phragmites australis). Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) of the acidic species in the runoff confirmed that the distribution of oil sands naphthenic acids and associated oil sand acids was dependent on the MFT treatment. Furthermore, FT-ICR MS studies of the acidic species in hydroponic systems revealed that there was no plant-mediated change in the electrospray ionization mass spectra of the runoff. O(o)-containing species were prevalent (>90%), O(o)S(s) were predominant (<10% relative abundance), and O(o)N(n) were least abundant in all runoff water samples. O(o)S(s) species were predominant in all the samples investigated. The heteroatomic classes present in runoff water at greater than 1% relative abundance include: O(2)N(1), O(3)N(1), O(2), O(2)S(1) O(3), O(3)S(1), O(4), O(4)S(1), O(5), O(5)S(1), O(6), O(6)S(1), O(7), O(7)S(1), O(8) and O(8)S(1). Assuming the same response factor for all O(o) species, the O(4) class, presumably dicarboxylic acids, was generally more prevalent than the O(2) class in all samples. The O(2) class is indicative of classical naphthenic acids. However, dicarboxylic acids will form negative ions more readily than the monocarboxylic acids as there are two acidic hydrogens available for formation of these species.
RESUMO
During reclamation the water associated with the runoff or groundwater flushing from dry stackable tailings technologies may become available to the reclaimed environment within an oil sands lease. Here we evaluate the performance of the emergent macrophyte, common reed (Phragmites australis), grown in chemically amended mature fine tailings (MFT) and simulated runoff/seepage water from different MFT drying treatments. The present study also investigated the phytotoxicity of the concentration of oil sands naphthenic acids (NAs) in different MFT drying chemical treatments, in both planted and unplanted systems. We demonstrate that although growth was reduced, the emergent macrophyte common reed was capable of growing in diluted unamended MFT runoff, as well as in diluted runoff from MFT amended with either 0.25% lime and gypsum or 0.5% gypsum. Common reed can thus assist in the dewatering process of oil sands MFT. However, simulated runoff or seepage waters from chemically amended and dried MFT were phytotoxic, due to combined levels of salts, naphthenic acids and pH. Phytoremediation of runoff water/ground water seepage from dry-land applied MFT will thus require pre-treatment in order to make conditions more favorable for plant growth.
Assuntos
Biodegradação Ambiental/efeitos dos fármacos , Ácidos Carboxílicos/toxicidade , Resíduos Industriais/efeitos adversos , Petróleo , Poaceae/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Compostos de Cálcio/química , Sulfato de Cálcio/química , Ácidos Carboxílicos/química , Ácidos Carboxílicos/metabolismo , Resíduos Industriais/análise , Óxidos/química , Poaceae/crescimento & desenvolvimento , Poaceae/metabolismo , Movimentos da Água , Poluentes Químicos da Água/química , Poluentes Químicos da Água/metabolismo , Abastecimento de Água/análise , Áreas AlagadasRESUMO
Naphthenic acids (NAs) are composed of alkyl-substituted acyclic and cycloaliphatic carboxylic acids and, because they are acutely toxic to fish, are of toxicological concern. During the caustic hot-water extraction of oil from the bitumen in oil sands deposits, NAs become concentrated in the resulting tailings pond water. The present study investigated if dissipation of NAs occurs in the presence of hydroponically grown emergent macrophytes (Typha latifolia, Phragmites australis, and Scirpus acutus) to determine the potential for phytoremediation of these compounds. Plants were grown with oil sands NAs (pKa approximately 5-6) in medium at pH 7.8 (predominantly ionized NAs) and pH 5.0 (predominantly nonionized NAs) to determine if, by altering their chemical form, NAs may be more accessible to plants and, thus, undergo increased dissipation. Whereas the oil sands NA mixture in its nonionized form was more toxic to wetland plants than its ionized form, neither form appeared to be sequestered by wetland plants. The present study demonstrated that plants may selectively enhance the dissipation of individual nonionized NA compounds, which contributes to toxicity reduction but does not translate into detectable total NA dissipation within experimental error and natural variation. Plants were able to reduce the toxicity of a NA system over 30 d, increasing the median lethal concentration (LC50; % of hydroponic solution) of the medium for Daphnia magna by 23.3% +/- 8.1% (mean +/- standard error; nonionized NAs) and 37.0% +/- 2.7% (ionized NAs) as determined by acute toxicity bioassays. This reduction in toxicity was 7.3% +/- 2.6% (nonionized NAs) and 45.0% +/- 6.8% (ionized NAs) greater than that in unplanted systems.
Assuntos
Ácidos Carboxílicos/toxicidade , Cyperaceae/efeitos dos fármacos , Poaceae/efeitos dos fármacos , Poluentes do Solo/toxicidade , Typhaceae/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Áreas Alagadas , Ácidos Carboxílicos/análise , Cyperaceae/crescimento & desenvolvimento , Cyperaceae/metabolismo , Monitoramento Ambiental , Íons/química , Poaceae/crescimento & desenvolvimento , Poaceae/metabolismo , Poluentes do Solo/análise , Testes de Toxicidade , Typhaceae/crescimento & desenvolvimento , Typhaceae/metabolismo , Poluentes Químicos da Água/análiseRESUMO
Mass spectrometry is a common tool for studying the fate of complex organic compound mixtures in oil sands processed water (OSPW), but a comparison of low-, high- ( approximately 10 000), and ultrahigh-resolution ( approximately 400 000) instrumentation for this purpose has not previously been made. High-resolution quadrupole time-of-flight mass spectrometry (QTOF MS) and ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), with negative-ion electrospray ionization, provided evidence for the selective dissipation of components in OSPW. Dissipation of oil sands naphthenic acids (NAs with general formula C(n)H(2n+z)O(2) where n is the number of carbon atoms, and Z is zero or a negative even number describing the number of rings) was masked (by components such as fatty acids, O(3), O(5), O(6), O(7), SO(2), SO(3), SO(4), SO(5), SO(6), and NO(4) species) at low resolution (1000) when using a triple quadrupole mass spectrometer. Changes observed in the relative composition of components in OSPW appear to be due primarily to the presence of plants, specifically cattails (Typha latifolia) and their associated microorganisms. The observed dissipation included a range of heteratomic species containing O(2), O(3), O(4), and O(5), present in Athabasca oil sands acid extracts. For the heteratomic O(2) species, namely naphthenic acids, an interesting structural relationship suggests that low and high carbon number NAs are dissipated by the plants preferentially, with a minimum around C(14)/C(15). Other heteratomic species containing O(6), O(7), SO(2), SO(3), SO(4), SO(5), SO(6), and NO(4) appear to be relatively recalcitrant to the cattails and were not dissipated to the same extent in planted systems.
Assuntos
Ácidos Carboxílicos/análise , Cromatografia Líquida de Alta Pressão/métodos , Óleos/química , Raízes de Plantas/química , Poluentes do Solo/análise , Espectrometria de Massas por Ionização por Electrospray/métodos , Typhaceae/química , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Differences in dissipation and phytotoxicity were measured for two naphthenic acid mixtures in hydroponically grown emergent macrophytes (Typha latifolia, Phragmites australis, and Scirpus acutus). One of the naphthenic acid (NA) mixtures was extracted from tailings pond water of an oil sands operation in Fort McMurray, Alberta, Canada. The other mixture was a commercially available NA mixture. While the oil sands NA mixture was less phytotoxic to wetland plants compared to the commercially available NA mixture, they were not sequestered by wetland plants like their commercial NA counterparts. The small loss of commercial NAs from the spiked hydroponic system appeared to be selective and dependant on the specific NA compound. The results of this study indicate that plants alone may not mitigate NAs from oil sands tailings pond water. In addition, caution should be taken when making predictions on the environmental fate of oil sands naphthenic acids when using commercial NAs as surrogates.
Assuntos
Ácidos Carboxílicos/toxicidade , Resíduos Industriais/efeitos adversos , Magnoliopsida/efeitos dos fármacos , Petróleo , Poluentes Químicos da Água/toxicidade , Ácidos Carboxílicos/metabolismo , Hidroponia , Magnoliopsida/crescimento & desenvolvimento , Magnoliopsida/metabolismo , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Brotos de Planta/efeitos dos fármacos , Brotos de Planta/crescimento & desenvolvimento , Brotos de Planta/metabolismo , Dióxido de Silício , Água/metabolismo , Poluentes Químicos da Água/metabolismoRESUMO
Through their diet, herbivores inhabiting contaminated sites may be chronically exposed to a variety of aryl hydrocarbons (e.g., dioxins and polycyclic aromatic hydrocarbons [PAHs]). However, little is known about how differences in morphology and physiology among plant species alter the environmental accumulation of aryl hydrocarbons or their release and subsequent activity in the gastrointestinal tract of herbivores after ingestion. In the present study, the activity of aryl hydrocarbons during digestion was examined using six Arctic plant species growing in impacted and reference sites near Inuvik, Northwest Territories, Canada. The plant species studied were black spruce (Picea mariana), labrador tea (Ledum groenlandicum), bog birch (Betula glandulosa), green alder (Alnus crispa), water sedge (Carex aquatilis), and little-tree willow (Salix arbusculoides). Plants were digested using a simulator of the upper digestive tract, and aryl hydrocarbon release was evaluated using an aryl hydrocarbon-receptor assay. Bioaccessible aryl hydrocarbon activity varied among the plant species tested. The species with the greatest activity was green alder, and the species with the least activity was black spruce. Further investigation revealed that digested plant extracts may antagonize the aryl hydrocarbon receptor and prevent bioactivation of the aryl compound benzo[a]pyrene. Thus, PAH risk from the ingestion of vegetation varies among plant species and may depend on antagonists present in the vegetation.