Quantification of 68 elements in river water monitoring samples in single-run measurements.

Triple-quadrupole inductively coupled plasma mass spectrometry (ICP-QQQ-MS) is a unique analytical technique which is, next to speciation analyses, applied for the determination of total element concentrations in several matrices. Due to its wide linear range, short analysis times, and the collision-reaction gas technology, it is capable of addressing a high number of analytes in a single run with sufficient low limits of quantification for river water monitoring. Over the last decades, the focus of the environmental monitoring changed from "traditional" and regulated analytes to elements of possibly rising concern from new applications such as the so-called technology-critical elements (TCE). By widening the analytical window of this method for applications in networks of future river water monitoring, a better understanding of natural transport processes and global biogeochemical element cycles will be established and the total number of methods can be reduced. During method development and validation, certified reference materials, calibration check solutions, and spiked river water samples from 12 major German rivers covering different catchment areas were measured and evaluated with the three cell gases He, H2 and O2. The method delivers a best as possible undisturbed simultaneous determination for 68 out of 71 target analytes with recoveries in an accepted range of 80-120% for river water samples (dissolved fraction; <0.45 µm). After comprehensive evaluation, we offer a novel best-practice multi-element method for river water monitoring with the goal of fostering the exchange and discussion between practitioners in long-term river monitoring. It enables the readers to create their own methods based on the scientific needs to monitor elemental "fingerprints" of rivers and their catchments.

Making waves: Time for chemical surface water quality monitoring to catch up with its technical potential.

A comprehensive real-time evaluation of the chemical status of surface water bodies is still utopian, but in our opinion, it is time to use the momentum delivered by recent advanced technical, infrastructural, and societal developments to get significantly closer. Procedures like inline and online analysis (in situ or in a bypass) with close to real-time analysis and data provision are already available in several industrial sectors. In contrast, atline and offline analysis involving manual sampling and time-decoupled analysis in the laboratory is still common practice in aqueous environmental monitoring. Automated tools for data analysis, verification, and evaluation are changing significantly, becoming more powerful with increasing degrees of automation and the introduction of self-learning systems. In addition, the amount of available data will most likely in near future be increased by societal awareness for water quality and by citizen science. In this analysis, we highlight the significant potential of surface water monitoring techniques, showcase "lighthouse" projects from different sectors, and pin-point gaps we must overcome to strike a path to the future of chemical monitoring of inland surface waters.

Arsenic metabolism in technical biogas plants: possible consequences for resident microbiota and downstream units.

The metal(loid) and in particular the Arsenic (As) burden of thirteen agricultural biogas plants and two sewage sludge digesters were investigated together with the corresponding microbial consortia. The latter were characterized by ARISA (automated ribosomal intergenetic spacer analysis) and next generation sequencing. The consortia were found to cluster according to digester type rather than substrate or metal(loid) composition. For selected plants, individual As species in the liquid and gaseous phases were quantified, showing that the microorganisms actively metabolize and thereby remove the As from their environment via the formation of (methylated) volatile species. The As metabolites showed some dependency on the microbial consortia, while there was no statistical correlation with the substrate mix. Finally, slurry from one agricultural biogas plant and one sewage sludge digester was transferred into laboratory scale reactors ("satellite reactors") and the response to a defined addition of As (30 and 60 µM sodium arsenite) was studied. The results corroborate the hypothesis of a rapid conversion of dissolved As species into volatile ones. Methanogenesis was reduced during that time, while there was no discernable toxic effect on the microbial population. However, the utilization of the produced biogas as replacement for natural gas, e.g. as fuel, may be problematic, as catalysts and machinery are known to suffer from prolonged exposure even to low As concentrations.

Moss bag monitoring as screening technique to estimate the relevance of methylated arsine emission.

Volatile arsenic (As) species, like arsine, mono-, di-, and trimethylarsine (AsH3, MeAsH2, Me2AsH, Me3As) are difficult to sample in remote areas without sophisticated equipment. The application of moss bags is an easy-to-apply screening technique which has been used for trapping total (mostly particulate) As from different emission sources before. We evaluated its potential for additional volatile As species screening. We found Me2AsH and Me3As in N2 atmosphere to be quantitatively trapped on the mosses and to be recoverable as their respective pentavalent acids (and/or oxyanions) when ground mosses were heated for 90min at 90°C in 0.1M HNO3/3% H2O2. MeAsH2 was trapped partially while AsH3 was not trapped. The most likely mechanism is covalent bonding to the moss surface. While moss monitoring does not replace more sophisticated techniques for volatile As sampling, it can easily be used as screening technique to determine whether besides particulate As volatile methylated As species could have any relevance in environments with yet unknown As emissions.

Investigation of poly(γ-glutamic acid) production via online determination of viscosity and oxygen transfer rate in shake flasks.

BACKGROUND: Poly(γ-glutamic acid) (γ-PGA) is a biopolymer with many useful properties making it applicable for instance in food and skin care industries, in wastewater treatment, in biodegradable plastics or in the pharmaceutical industry. γ-PGA is usually produced microbially by different Bacillus spp. The produced γ-PGA increases the viscosity of the fermentation broth. In case of shake flask fermentations, this results in an increase of the volumetric power input. The power input in shake flasks can be determined by measuring the torque of an orbitally rotating lab shaker. The online measurement of the volumetric power input enables to continuously monitor the formation or degradation of viscous products like γ-PGA. Combined with the online measurement of the oxygen transfer rate (OTR), the respiration activity of the organisms can be observed at the same time. RESULTS: Two different Bacillus licheniformis strains and three medium compositions were investigated using online volumetric power input and OTR measurements as well as thorough offline analysis. The online volumetric power input measurement clearly depicted changes in γ-PGA formation due to different medium compositions as well as differences in the production behavior of the two investigated strains. A higher citric acid concentration and the addition of trace elements to the standard medium showed a positive influence on γ-PGA production. The online power input signal was used to derive an online viscosity signal which was validated with offline determined viscosity values. The online measurement of the OTR proved to be a valuable tool to follow the respiration activity of the cultivated strains and to determine its reproducibility under different cultivation conditions. CONCLUSIONS: The combination of the volumetric power input and the OTR allows for an easy and reliable investigation of new strains, cultivation conditions and medium compositions for their potential in γ-PGA production. The power input signal and the derived online viscosity directly reflect changes in γ-PGA molecular weight and concentration, respectively, due to different cultivation conditions or production strains.

Spin fluctuations in normal state CeCu2Si2 on approaching the quantum critical point.

We report on the magnetic excitation spectrum in the normal state of the heavy-fermion superconductor CeCu92)Si(2) on approaching the quantum critical point (QCP). The magnetic response in the superconducting state is characterized by a transfer of spectral weight to energies above a spin excitation gap. In the normal state, a slowing-down of the quasielastic magnetic response is observed, which conforms to the scaling expected for a QCP of spin-density-wave type. This interpretation is substantiated by an analysis of specific heat data and the momentum dependence of the magnetic excitation spectrum. Our study represents the first direct observation of an almost critical slowing-down of the normal state magnetic response at a QCP when suppressing superconductivity. The results strongly imply that the coupling of Cooper pairs in CeCu(2)Si(2) is mediated by overdamped spin fluctuations.

Socialization in health education: encouraging an integrated interprofessional socialization process.

There is limited research on how health professionals are currently socialized to work interprofessionally. As part of a large-scale initiative funded by Health Canada, this report adds to our understanding of socialization and how it can prepare the health care student for an interprofessional health care environment. Data were collected through semistructured individual and group interviews with 83 respondents (i.e., faculty, students, health care and academic administrators, and health care providers) at seven clinical sites and five academic institutions throughout Alberta. Respondents indicated that socialization prepares health care students for interprofessional environments by "building a professional identity" and through what we are labeling "interprofessional familiarization" (i.e., where the goal is to introduce students to the roles and function of other professionals outside their own discipline). While there is interprofessional familiarization, it is neither consistently espoused as important nor systematically embedded in curriculum and clinical placements. Interprofessional competency building is lacking during this preparatory phase, leaving students ill prepared to work in interprofessional health care environments. We argue there is a need for greater interprofessional socialization in education. By creating a deliberate, planned, and integrated interprofessional socialization process that is consistent across the health professions, barriers to interprofessional practice could be mitigated.

Role understanding and effective communication as core competencies for collaborative practice.

The ability to work with professionals from other disciplines to deliver collaborative, patient-centred care is considered a critical element of professional practice requiring a specific set of competencies. However, a generally accepted framework for collaborative competencies is missing, which makes consistent preparation of students and staff challenging. Some authors have argued that there is a lack of conceptual clarity of the "active ingredients" of collaboration relating to quality of care and patient outcomes, which may be at the root of the competencies issue. As part of a large Health Canada funded study focused on interprofessional education and collaborative practice, our goal was to understand the competencies for collaborative practice that are considered most relevant by health professionals working at the front line. Interview participants comprised 60 health care providers from various disciplines. Understanding and appreciating professional roles and responsibilities and communicating effectively emerged as the two perceived core competencies for patient-centred collaborative practice. For both competencies there is evidence of a link to positive patient and provider outcomes. We suggest that these two competencies should be the primary focus of student and staff education aimed at increasing collaborative practice skills.

How can frontline managers demonstrate leadership in enabling interprofessional practice?

Interprofessional practice has been proposed to address many of the current health system challenges. Little attention has been paid to the role of frontline managers in supporting effective interprofessional practice. Qualitative interviews suggest that frontline managers can demonstrate leadership in enabling interprofessional practice by creating an organizational culture for interprofessional practice, eliminating barriers to collaboration, and acting as role models and facilitators. New management approaches may be needed to meet these demands.