The PHH-1V HIPRA vaccine: a new tool in the vaccination strategy against COVID-19.

OBJECTIVE: Vaccination against SARS-CoV-2 is essential to mitigate the personal, social and global impact of the coronavirus disease (COVID-19) as we move from a pandemic to an endemic phase. Vaccines are now required that offer broad, long-lasting immunological protection from infection in addition to protection from severe illness and hospitalisation. Here we present a review of the evidence base for a new COVID-19 vaccine, PHH-1V (Bimervax®; HIPRA HUMAN HEALTH S.L.U), and the results of an expert consensus. METHODS: The expert committee consisted of Spanish experts in medicine, family medicine, paediatrics, immunology, microbiology, nursing, and veterinary medicine. Consensus was achieved using a 4-phase process consisting of a face-to-face meeting during which the scientific evidence base was reviewed, an online questionnaire to elicit opinions on the value of PHH-1V, a second face-to-face update meeting to discuss the evolution of the epidemiological situation, vaccine programmes and the scientific evidence for PHH-1V and a final face-to-face meeting at which consensus was achieved. RESULTS: The experts agreed that PHH-1V constitutes a valuable novel vaccine for the development of vaccination programmes aimed towards protecting the population from SARS-CoV-2 infection and disease. Consensus was based on evidence of broad-spectrum efficacy against established and emerging SARS-CoV-2 variants, a potent immunological response, and a good safety profile. The physicochemical properties of the PHH-1V formulation facilitate handling and storage appropriate for global uptake. CONCLUSIONS: The physicochemical properties, formulation, immunogenicity and low reactogenic profile of PHH-1V confirm the appropriateness of this new COVID-19 vaccine.

Mechanical properties of friction induced nanocrystalline pearlitic steel.

Nanocrystalline structured variants of commercially available alloys have shown potential for boosting the mechanical properties of these materials, leading to a reduction in waste and thereby retaining feasible supply chains. One approach towards achieving these nanostructures resides in frictional treatments on manufactured parts, leading to differential refinement of the surface structure as compared to the bulk material. In this work the machining method is considered to be a testing platform for the formation and study of frictional nanostructured steel, assembly of which is stabilized by fast cooling of the produced chip. Analysis of the mechanical properties has shown extraordinary results at the surface, over 2000 MPa of strength on AISI1045 steel, more than three times the strength of the base material, demonstrating at the same time a reduction of 15% in the elastic modulus. The microscopic analysis suggests a reassembly of the elements in a new lattice of carbon supersaturated nano-ferrite.

Vacunación frente a Herpes Zóster en la Comunidad de Madrid / No disponible

No disponible

Calendario de vacunaciones sistemáticas del adolescente y adulto recomendado por la sociedad española de medicina preventiva, salud Pública e higiene (actualización del año 2014) / No disponible

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Clarificación combinada y evaluación sensorial de jugo de marañón (Anacardium occidentale L) / Combined clarification and sensory evaluation of cashew juice (Anacardium occidentale L)

Objetivo. Obtener jugo clarificado de marañón, evaluar sensorialmente jugos clarificados optimizados y establecer diferencias químicas entre el jugo integral y el clarificado. Materiales y métodos. Los pseudofrutos se separaron manualmente de la nuez, fueron seleccionados y lavados, luego se realizó escaldado y extracción del jugo. El jugo integral y el de mayor aceptación fueron caracterizados químicamente. Los jugos se evaluaron sensorialmente (aceptación) con una escala hedónica de 9 puntos y una prueba de ordenamiento por 30 catadores. Se empleó un diseño factorial de tres niveles combinado con la metodología de superficie de respuesta; las características químicas de los jugos se analizaron por prueba de homogeneidad de varianzas de Levene y la prueba T-Student de comparación de medias para muestras independientes. Resultados. Los jugos evaluados sensorialmente no presentaron diferencias estadísticas significativas entre sí (p≤0.05, p≤0.01); sin embargo el mayor porcentaje de aceptación fue del tratamiento 0.20% p/v Rapidasa® CX y 14.27 horas a 30°C, se presentaron diferencias estadísticas significativas para las variables pH, °Brix, azúcares reductores y ácido ascórbico (p≤0.05); además, el contenido de ácido ascórbico se redujo notablemente en un 41.01% con respecto al jugo inicial. Conclusiones. El jugo clarificado con alto contenido de vitamina C, obtenido por tratamiento enzimático, constituye una forma de aprovechamiento agroindustrial del pseudofruto, teniendo aceptación para su consumo y sin presentar astringencia, con buen sabor, aroma, mediante tratamiento 0.20% p/v Rapidasa® CX y 14.27 horas a 30°C.

Objective. Obtain clarified cashew juice, in order to sensorially evaluate clarified and optimized juice and establish chemical differences between the integral and the clarified juice. Materials and methods. The pseudo fruits were manually separated from the nut and were selected and washed, then scalded and the juice was extracted. The natural juice and the most accepted one were chemically characterized. Juices submitted to sensory evaluation (acceptance) with a 9-point hedonic scale and a test system for 30 tasters. A three-level factorial design combined with response surface methodology was used. The chemical characteristics of juices were analyzed by Levene’s test for homogeneity of variances and T-Student test for comparison of means for independent samples. Results. The evaluated juices displayed no statistically significant differences (p≤0.05, p≤0.01), but the highest percentage of acceptance was for the juice treated with 0.20% w/v Rapidase® CX and 14.27 hours at 30°C, differences were statistically significant for pH, °Brix, reducing sugars and ascorbic acid (p≤0.05), furthermore, the content of ascorbic acid was significantly reduced by 41.01% compared to the initial juice. Conclusions. The clarified juice with high vitamin C content, obtained by enzymatic treatment, is a way of agro-industrial use of the pseudo fruit, having been accepted for consumption, and non-astringent, with good flavor, aroma, by treatment with 0.20% w/v Rapidase® CX and 14.27 hours at 30°C.

Determinación de compuestos cianogénicos amigdalina y prunasina en semillas de almendras (prunus dulcis l) utilizando cromatografía liquida de alta resolución / Determination of cyanogenic compound amygdalin and prunasin in almond kernels (prunus dulcis l) by using liquid chromatography / Determinação de compostos cianogênicos amigdalina e prunasina em sementes e amêndoa (prunus dulcis miller) cromatografia líquida de alta resolução

El objetivo del presente trabajo fue aplicar un técnica para determinar y cuantificar por separado los compuestos cianogénicos que pueden estar presentes en la semilla de almendra madura (Prunus dulcis). Entre los métodos encontrados, se seleccionó la cromatografía de líquidos de alta resolución (HPLC), que permite la cuantificación de los glucósidos cianogénicos amigdalina y prunasina por separado, adecuando diferentes procedimientos de extracción como el tamaño de partículas que influye en el proceso de liofilización, donde a menor superficie mayor área de contacto para la sublimación. Se ensayaron muestras sin grasa y con grasa, utilizando los resultados con muestras con grasa, dados los resultados obtenidos. Se utilizó metanol 100% como extractante de los glucósidos cianogénicos, resultando una concentración de amigdalina máxima a partir un tiempo de extracción de 12 horas y como fase móvil acetonitrilo/agua (20:80), se obtiene amigdalina, con una concentración de 9,8 mg/100g de muestra seca. Los cromatogramas obtenidos presentan tiempo de retención (Tr), Amigdalina: 3,4 y Prunasina, 5,7, dos picos con excelente resolución, por lo tanto las condiciones anteriores se pueden utilizar para la identificación y cuantificación de amigdalina y prunasina.

The aim of this study was to apply a technique to identify and quantify separately also cyanogenic compounds that may be present in the mature seed almond (Prunus dulcis). Among the methods selected the chromatography of liquids of high resolution (HPLC), that permit the quantification of the glycosides for the separation process of Freeze Dry where there is less surface there is more contact to sublimation without fat samples, looking at the obtain results and supported by other investigations, the use of 100 % methanol extract as a mobile phase acetonitrile-water (80:20) the results obtained of the glycosides cyanogenics resulting in a concentration of maximum amygdalin from the time of extraction of twelve hours, amygdalin is obtained, with a concentration of 9,8 mg / 100 g of dry sample. The chromatograms obtained a time of retention (Tr), amygdalin 3,4 and prunasin 5,7 two peaks with excellent resolution, to the above conditions can be used for analysis by HPLC, identification and quantification of amygdalin and prunasina.

Neste trabalho, a técnica é aplicada para determinar e também para quantificar separadamente compostos cianogénicos que podem estar presentes na semente madura amêndoa (Prunus dulcis). Métodos encontrados é seleccionado de cromatografia líquida de alta eficiência (HPLC), que permite a quantificação dos glicosídeos separar adaptar diferentes técnicas de extracção, tais como o tamanho de partícula influencia o processo de liofilização, onde a área de superfície maior menor sublimação contacto com desengradas amostras de gordura e usando os resultados com amostras desengorduradas, Tendo em vista os resultados obtidos, e suportados por outras pesquisas metanol a 100 % foi usado como o agente de extracção e como fase móvel acetonitrilo/água (80:20) de glicósidos cianogénicos, resultando numa concentração elevada de amigdalina a partir de um tempo de extracção de 12 horas. Amigdalina é obtido, com uma concentração de 9,8 mg / 100 g de amostra seca. Os cromatogramas apresentados tempo de retenção (Tr), Amygdalin: 3,4 e prunasina 5,7 dois picos com excelente resolução, com as condições acima podem ser utilizados para a análise por HPLC. identificação e quantificação de amigdalina e prunasina.

Determinación d compuestos cianogénicos amigdaina y prunasina en semillas de almendras (prunus dulcis l) utilizando cromatografía liquida de alta resolución / Determination of cyanogenic compound amygdalin and prunasin in alond kernels (prrunus dulcis l) by using liquid chromatography / Detrminação de ccompostos cianogênicos amigdalina eprunasina em seementes e amêndoa (prunus dulcis miller) cromatografia líquida de alta resolução

El objetivo del presente trabajo fue aplicar un técnica para determinar y cuantificar por separado los compuestos cianogénicos que pueden estar presentes en la semilla de almendra madura (Prunus dulcis). Entre los métodos encontrados, se seleccionó la cromatografía de líquidos de alta resolución (HPLC), que permite la cuantificación de los glucósidos cianogénicos amigdalina y prunasina por separado, adecuando diferentes procedimientos de extracción como el tamaño de partículas que influye en el proceso de liofilización, donde a menor superficie mayor área de contacto para la sublimación. Se ensayaron muestras sin grasa y con grasa, utilizando los resultados con muestras con grasa, dados los resultados obtenidos. Se utilizó metanol 100% como extractante de los glucósidos cianogénicos, resultando una concentración de amigdalina máxima a partir un tiempo de extracción de 12 horas y como fase móvil acetonitrilo/agua (20:80), se obtiene amigdalina, con una concentración de 9,8 mg/100g de muestra seca. Los cromatogramas obtenidos presentan tiempo de retención (Tr), Amigdalina: 3,4 y Prunasina, 5,7, dos picos con excelente resolución, por lo tanto las condiciones anteriores se pueden utilizar para la identificación y cuantificación de amigdalina y prunasina.

The aim of this study was to apply a technique to identify and quantify separately also cyanogenic compounds that may be present in the mature seed almond (Prunus dulcis). Among the methods selected the chromatography of liquids of high resolution (HPLC), that permit the quantification of the glycosides for the separation process of Freeze Dry where there is less surface there is more contact to sublimation without fat samples, looking at the obtain results and supported by other investigations, the use of 100 % methanol extract as a mobile phase acetonitrile-water (80:20) the results obtained of the glycosides cyanogenics resulting in a concentration of maximum amygdalin from the time of extraction of twelve hours, amygdalin is obtained, with a concentration of 9,8 mg / 100 g of dry sample. The chromatograms obtained a time of retention (Tr), amygdalin 3,4 and prunasin 5,7 two peaks with excellent resolution, to the above conditions can be used for analysis by HPLC, identification and quantification of amygdalin and prunasina.

Neste trabalho, a técnica é aplicada para determinar e também para quantificar separadamente compostos cianogénicos que podem estar presentes na semente madura amêndoa (Prunus dulcis). Métodos encontrados é seleccionado de cromatografia líquida de alta eficiência (HPLC), que permite a quantificação dos glicosídeos separar adaptar diferentes técnicas de extracção, tais como o tamanho de partícula influencia o processo de liofilização, onde a área de superfície maior menor sublimação contacto com desengradas amostras de gordura e usando os resultados com amostras desengorduradas, Tendo em vista os resultados obtidos, e suportados por outras pesquisas metanol a 100 % foi usado como o agente de extracção e como fase móvel acetonitrilo/água (80:20) de glicósidos cianogénicos, resultando numa concentração elevada de amigdalina a partir de um tempo de extracção de 12 horas. Amigdalina é obtido, com uma concentração de 9,8 mg / 100 g de amostra seca. Os cromatogramas apresentados tempo de retenção (Tr), Amygdalin: 3,4 e prunasina 5,7 dois picos com excelente resolução, com as condições acima podem ser utilizados para a análise por HPLC. identificação e quantificação de amigdalina e prunasina.

Recomendaciones de vacunación antineumocócica en el adulto por indicación médica / No disponible

No disponible

Aprovechamiento de las características nutricionales del almendro de la india (Terminalia catappa L. ) como suplemento en la alimentación animal / Nutritional characteristics of the Indian almond Terminalia catappa L as a supplement in animal feeding

Objetivo. Evaluar nutricionalmente la semilla del almendro de la india (Terminalia catappa)para tres estados de madurez: E1 verde; E2 intermedio y E3 maduro, como una alternativade suplemento en la alimentación animal. Materiales y métodos. Se determinaron lascaracterísticas fisicoquímicas de la pulpa de los frutos y se les extrajo la semilla a la cual seles realizó el análisis bromatológico mediante los métodos oficiales de análisis químico AOAC.A la grasa extraída de la semilla se le realizó un perfil de composición de ácidos grasos pormedio de la técnica de cromatografía gaseosa acoplada a espectrometría de masas.Resultados. Para la semilla se obtuvo en promedio: grasa 54%, proteína 24%, ceniza 4%,fibra 12% y humedad 45%. Los datos se procesaron utilizando un análisis de varianza(ANOVA). Los estados de madurez, presentaron diferencias estadísticas (p≤0,05) decomposición para la semilla; mientras que para las variables fisicoquímicas de la pulpa, pH,acidez y grados Brix, arrojaron resultados no significativos. El perfil de ácidos grasos delaceite de la semilla para E1, E2 y E3, mostraron similitud en ciertos componentes. Conclusión.Teniendo en cuenta la composición nutricional de la semilla de almendro (Terminalia catappa)y su elevado valor en proteína (24%), grasa (54%), fibra (12%), es posible su aprovechamientopara la industrialización e inclusión en concentrados para la alimentación animal.

Immunogenicity and safety of an adjuvanted hepatitis B vaccine in pre-hemodialysis and hemodialysis patients.

BACKGROUND: Due to their impaired immune system, patients with renal insufficiency have a suboptimal response to hepatitis B (HB) vaccination and frequent boosters are needed to maintain protection. GlaxoSmithKline Biologicals has developed a HB vaccine containing a new adjuvant system AS04 for use in this immunocompromised patient population. METHODS: In an open, randomized clinical trial conducted in pre-hemodialysis (documented creatinine clearance < or =30 mL/min) and hemodialysis patients, over 15 years of age and naïve for HB, the immunogenicity and safety of single doses of HB-AS04 (Fendrix, GlaxoSmithKline Biologicals) were compared to double doses of commercially available HB vaccine (Engerix, GlaxoSmithKline Biologicals) administered at 0, 1, 2, and 6 months, and followed-up for 36 months. RESULTS: The HB-AS04 vaccine elicited a more rapid onset of protection than the currently licensed vaccine for this particular population, with 74% versus 52% of subjects seroprotected at month 3. After the vaccination course, seroprotection rates increased to 91% versus 84% in the HB-AS04 and standard vaccine groups, respectively. Differences persisted up to 36 months post-vaccination (73% vs. 52%, respectively). Antibody concentrations were higher following the HB-AS04 vaccine at all post-vaccination time points. During the follow-up, significantly fewer subjects primed with the HB-AS04 vaccine needed a booster dose as a consequence of anti-HBs loss below seroprotective levels (11/62 subjects in the HB-AS04 group vs. 22/57 subjects in the standard vaccine group, respectively, P = 0.014). The HB-AS04 was more locally reactogenic than the standard immunization regimen, with pain at the injection site occurring with 41% of HB-AS04 doses versus 19% of standard vaccine doses. The occurrence of grade 3 pain was less than 1% in both groups and all events resolved within the 4-day follow-up period. CONCLUSION: The improved immunogenicity profile and clinically acceptable reactogenicity of HB-AS04 vaccine are of key importance to provide a more rapid, enhanced, and longer seroprotection to these immunocompromised patients at risk for HB infection.

[Prevalence of antibodies against tetanus, diphtheria and Bordetella pertussis in health care professionals]. / Prevalenica de anticuerpos frente a tétanos, difteria y Bordetella pertussis en profesionales sanitarios.

AIM: The aim of the present study was to determine the prevalence of antibodies versus tetanus, diphtheria and Bordetella pertussis in a population of health care personnel. PATIENTS AND METHODS: A transversal study was carried out in a population of healthy health care professionals from two hospitalary centers in the Community of Madrid (Spain). The ELISA method was used for antibody determinations. The determination of antibodies versus tetanus and diphtheria was performed in a total of 344 individuals (mean age 32 years) and that of whooping cough in 104 subjects (mean age 23 years). RESULTS: The global prevalence of tetanus, diphtheria and whooping cough was of 76.5, 48 and 57%, respectively. The prevalence of antibodies versus tetanus was significantly greater in males than in females. CONCLUSIONS: Revaccination versus tetanus and diphtheria is recommended every 10 years to guarantee protector antibody concentrations in adults. With regard to whooping cough, revaccination could be a strategy to consider in the future, particularly in risk groups in contact with cases of this disease.