[Use of combined detection of hepatitis C virus core antigen and antibodies to reduce the serological window-phase]. / Intérêt du dépistage combiné de l'antigène de capside et des anticorps du virus de l'hépatite C dans la réduction de la fenêtre sérologique.

OBJECTIVES: In this study, we aimed at evaluating the performances of a combined assay for the detection of hepatitis C virus core antigen and antibodies and comparing this test with conventional third generation Elisa. MATERIAL AND METHODS: Two hundred forty-one samples were included in this study and tested by Monolisa HCV Ag-Ab ULTRA, Biorad and compared to Monolisa Anti-HCV Plus. A comparative study was performed on a HCV seroconversion panel (Monolisa anti-HCV Plus, Biorad; Innotest HCV Ab IV, Innogenetics and Murex anti-HCV, Abbott). False positive samples were detected with western blot assay (INNO-LIA HCV Ab III, Innogenetics). Two anti-HCV negative haemodialysis patients with rise in ALT have been tested for RNA detection (Amplicor v2.0, Roche). RESULTS: Results obtained with Biorad Ag-Ab were in agreement with third generation ELISA on HCV seroconversion panel. From anti-HCV negative patients, four samples were found low positive with HCV Ag-Ab. Two anti-HCV negative haemodialysis patients/HCV RNA positive were also negative with HCV Ag-Ab and 13 low positive samples with Biorad Ab were found negative with Ag-Ab. CONCLUSION: The HCV Ag-Ab assay has a high specificity and sensitivity comparatively to conventional ELISA; but in our study we don't prove the reduction of the "serologic window" for detection of anti-HCV antibodies.

[Successful treatment of Echovirus 27 meningoencephalitis in agammaglobulinaemia with intraventricular injection of gammaglobulin. A case report]. / Guérison d'une méningo-encéphalite à Echovirus 27 par immunoglobulines intraventriculaires au cours d'une agammaglobulinémie. A propos d'un cas.

BACKGROUND: Meningoencephalitis due to enteroviruses is particularly serious when occurring in patients with agammaglobulinaemia. This disease is associated with a high mortality and a significant risk for neurological sequelae in such circumstances. We report here a new case treated with intraventricular immunoglobulin, whose evolution was favourable. CASE REPORT: A three-year-old boy with agammaglobulinaemia, while he was treated with gammaglobulin with an IgG residual concentration of 10 g/l, presented neurological symptoms related to Echovirus 27 meningo encephalitis. Under treatment with intraventricular gammaglobulin by means of an Ommaya reservoir, the patient recovered. CONCLUSION: Favourable evolution is rare in meningo encephalitis in agammaglobulinaemic patients. Prognosis depends on an early diagnosis and on the extent of dissemination of the infection. Intraventricular gammaglobulin administration may contribute to a favourable outcome.

First molecular characterization of HIV-1 Tunisian strains.

In order to identify HIV-1 subtypes circulating in Tunisia, blood specimens from 25 HIV-1 infected Tunisian patients were collected. Proviral HIV-1 DNA was genotyped by sequence analysis of the C2-V3 env region. HIV-1 subtypes were determined in 21 DNA sequences: 20 were of subtype B and one was a circulating recombinant form (CRF02 AG). Subtype B largely dominates the epidemiology of HIV-1 infection in Tunisia, suggesting the probable imported origin of HIV-1 infection, but further studies are needed to investigate the potential diversification of HIV-1 isolates in Tunisia.

[Clinical aspects and etiology of lower respiratory tract infections in hospitalized children]. / Aspect clinique et etiologique des infections respiratoires basses de l'enfant en milieu hospitalier.

The authors report a prospective study conducted in one of the general pediatrics unit. One hundred one children, aged 20 days to 12 years, admitted with acute lower respiratory tract infections have been studied on clinical paraclinicals and etiology grounds. All children had a chest Ray roentgenogram, a total blood cell count and C-reactive protein. The detection of a viral agent in nasopharyngeal aspirate by immunofluorescent technic were performed. 85% of patients have bronchiolitis, the chest--Rays showed an abnormal pictures in 84.2% of cases, an anaemia was present in 54.7%. Viral agent as identified in 58 patients, the RSV indicated in 56 cases. Bacterial infection as present in 57 of the 101 children. A mixed viral-bacterial infection as documented in 28 cases. Among bacterial causes the predominant agent as Haemophilus influenzae 24, Branhamella Catarrhalis 17, streptococcus pneumoniae in 16 cases. Early empiric antibiotic as performed. All patients recovered uneventfully.

[Evaluation of the frequency of oncogenic HPV in venereal condyloma. Prospective study of 24 patients]. / Evaluation de la fréquence des HPV à risque oncogène au cours des végétations vénériennes. Etude prospective de 24 patients.

UNLABELLED: Human papillomavirus (HPV) infection tend to be the most prevalent sexually transmitted disease (STD), and "high-risk" HPV types are correlated to intra-epithelial neoplasia, especially cervical cancer. The aim of this study was to evaluate the frequency of "high risk" HPV types in condyloma. PATIENTS AND METHODS: A non randomised prospective study was performed, including 24 patients with condyloma examined in the department of Dermatology of Charles Nicolle's hospital. Molecular hybridisation method was realised for all patients to identify HPV type. RESULTS: Mean age of the 24 patients was 37.8 years Ratio (male/female): 1.4 In 21 patients HPV DNA was detected 2 women had "high risk" HPV type (9.5%).

Molecular epidemiology of poliovirus infection in Tunisia.

This report is an overview of poliomyelitis surveillance in Tunisia from 1991 to 1996. In all, 2088 stool specimens, collected from 152 acute flaccid paralysis (AFP) cases and from 1747 of their healthy contacts were investigated. Virus isolation was done systematically in RD and HEp-2C cell lines and isolated viruses were typed by sero-neutralisation as polioviruses or non-polio enteroviruses. Poliovirus isolates were analysed systematically for their wild or vaccine-related origin by two methods--one based on antigenic differences and one on genetic differences between strains. All type 2 polioviruses were vaccine-related and most wild viruses belonged to polio serotype 3. Wild polio type 3 viruses were detected in 1991 and 1992 in six cases of paralytic polio. A silent circulation of wild polio 1 and wild polio 3 was detected in 1994. No wild virus was detected in Tunisia from 1995 onwards. Wild polioviruses were sequenced and compared with Tunisian wild strains isolated during the 1980s, as well as other genotypes from the international database. These investigations revealed a single Tunisian polio 3 genotype that has been circulating from 1985 to 1994 and two different polio 1 genotypes. These results reflect effective control strategies within the country and contribute to the improvement of the polio eradication programme effectiveness at national and global levels.

[Frequency and characteristics of Campylobacter jejuni-coli diarrhea in Tunisia]. / Fréquence et caractères de la diarrhée à Campylobacter jejuni-coli en Tunisie.

Because importance of Campylobacter jejuni/coli in human disease, we studied frequency, clinical and biological symptoms of Campylobacter enteritis. Investigation has been realised on 216 cases of diarrhoea seen outside the hospital and aged from 4 days to 60 years, and 512 cases of hospitalised diarrhoea. Outside the hospital, this bacteria has the same frequency as Shigella and Salmonella (3-5%). Inside the hospital, it is rare. Outside the hospital, this bacteria is isolated in babies and children. Inside the hospital, it affects children and adults. In the hospital, clinical and biological symptoms of Campylobacter enteritis are more serious: Campylobacter predominant in stools, presence of leukocytes for all patients, and no other pathogenic organisms associated.