Fungal derived herbicidal metabolite loaded starch-chitosan-gum acacia-agar based bio composite: Preparation, characterization, herbicidal activity, release profile and biocompatibility.

Biocomposites based on starch- gum acacia- agar, chitosan- starch- agar, starch- poly vinyl alcohol- agar were synthesized by simple, green route principles and the various characterization techniques like fourier infrared spectroscopy, SEM revealed the highly stable micro dimenstional that specially interacted with functional groups of polymers -herbicidal metabolites. Respective biocomposite was prepared by mixing equal volume of the selected polymer (1;1;1 ratio) with known concentration (100 mg of in distilled water followed by the addition of reconstituted herbicidal metabolites (100 mg or 0.1 g). Though all the biocomposites were capable of inducing herbicidal effect, notable impact was recorded in chitosan- starch- gum acacia treatment. In this case, the necrotic lesions were initiated at the early incubation period (6 h), progressively developing into dark brownish black lesions with 30.0 mm diameter. Release profile of the metabolites from the respective composite was also under in vitro and soil assay. Release profile study under in vitro and soil condition showed the sustained or controlled manner in distilled water and ethyl acetate treatment. No sign of toxic effect on the soil, parameters plant growth, rhizobacteria and peripheral blood cells clearly revealed the best biocompatibility of the presently proposed biocomposite.

Noteworthy biocompatibility of effective microorganisms (EM) like microbial beneficial culture formulation with metal and metal oxide nanoparticles.

The present study evaluates the biocompatibility of silver and zinc oxide nanoparticles with various effective microorganisms (EM), like beneficial microbial formulations. The respective nanoparticle was synthesised by chemical reduction of metal precursor with reducer via simple route green technology principles. The synthesised nanoparticles were characterised by UV visible spectroscopy, scanning electron microscopy (SEM), and X-ray diffraction (XRD) studies, revealing highly stable, nanoscale particles with marked crystallinity. EM-like beneficial cultures composed of viable cells of Lactobacillus lactis, Streptomyces sp, Candida lipolytica, and Aspergillus oryzae were formulated with rice bran, sugarcane syrup, and groundnut cake. The respective formulation was inoculated into the nanoparticles amalgamated pots raised with green gram seedlings. Biocompatibility was determined by measuring plant growth parameters of a green gram at pre-determined periods associated with enzymatic antioxidants like catalase (CAT), superoxide dismutase (SOD), and glutathione S transferase (GST) levels. Most significantly, the expression level of these enzymatic antioxidants level was also investigated by quantitative real-time polymerase chain reaction (qRT-PCR). The impact of the soil conditioning effect on soil nutrients like nitrogen, phosphorous, potassium, organic carbon, soil enzymes glucosidases, and ß-xylosidases activity was also studied. Among the formulation, rice bran-groundnut cake-sugar syrup formulation recorded the best biocompatibility. This formulation showed high growth promotion, soil conditioning effect and no impact on the oxidative stress enzymes genes that revealed the best compatibility of nanoparticles. This study concluded that biocompatible, eco-friendly formulations of microbial inoculants could be used for the desirable agro active properties that show extreme tolerance or biocompatibility to the nanoparticles. This present study also suggests the utilisation of the above said beneficial microbial formulation and metal-based nanoparticles with desirable agro active properties in a synergistic manner due to their high tolerance or compatibility towards the metal or metal oxide nanoparticles.

Evaluation of methyl orange adsorption potential of green synthesized chitosan-silver nanocomposite (CS-AgNC) and its notable biocompatibility on freshwater Tilapia (Oreochromis nitoticus).

Nanomaterials mainly nanocomposites possess unique physical and chemical properties which makes them superior and indispensable. Though much research has been focused on the properties and application of nanocomposites, the eco-toxicity assessment is one among top priority, which aims to protect the population of concerned biological component and their ecosystem. With this objective, the present study has undertaken an initiation to evaluate the efficacy of chitosan-silver nanocomposite for methyl orange adsorption property (CS-AgNC) and also assessed the toxicity impact on growth parameters of freshwater Tilapia. Batch in vitro studies showed that all the tested dosages of the nanocomposite were effectively adsorbing maximum concentration of methyl orange. The synthesized nanocomposite was administrated to the tested fishes followed by the determination of various growth, nutritional parameters, gene expression of enzymatic antioxidants and liver, and intestinal tissues histology. Obtained results indicated that nanocomposite treatment was not projected as a toxic impact on all the tested growth, and nutritional parameters. Histology study showed that the exposure of Tilapia to nanocomposite has not shown any detrimental effect on antioxidants gene expression and liver, intestinal tissue architecture. Hence, all these findings indicated that chitosan-silver nanocomposite prepared in our present system was found to be biocompatible which suggested the possible utilization and release of the nanocomposite into the divergent ecosystem without affecting non-target organisms (NTO).

Green chemistry principles for the synthesis of anti fungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC-NT) against food spoilage fungal strain Aspergillus ochraceopealiformis and its marked Congo red dye adsorption efficacy.

In this present study, a highly stable gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC-NT) was prepared via green science principles under in vitro conditions. Various characterisation techniques reveal highly stable structural, functional properties of the synthesised nanocomposite with marked antifungal activity and adsorption efficacy against congo red dye. The antifungal activity was investigated against the fungal strain Aspergillus ochraceopealiformis isolated from spoiled, expired bread. The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite. Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain. The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR). Among the various treatment, a notable reduction in all the tested marker genes expression was recorded in the nanocomposite treated fungal strain. Release profile studies using different solvents reveals sustained or controlled release of natamycin at the increasing periods. The synthesised nanocomposite's high safety or biocompatibility was evaluated with the Wistar animal model by determining notable changes in behavioural, biochemical, haematological and histopathological parameters. The synthesised nanocomposite did not exhibit any undesirable changes in all the tested parameters confirming the marked biosafety or biocompatibility. The nanocomposite was coated on the bread packaging material. The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied. Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) studies reveal that the nanocomposite was effectively coated on the packaging material without changing size, shape, and functional groups. No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite's marked safety. The complete absence of the fungal growth also indicates the uniqueness of the nanocomposite. Further, the sorption studies revealed the utilisation of Langmuir mechanism and pseudo II order model successfully The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent and adsorbent of toxic chemicals.

Gum acacia PEG iron oxide nanocomposite (GA-PEG-IONC) induced pharmacotherapeutic activity on the Las R gene expression of Pseudomonas aeruginosa and HOXB13 expression of prostate cancer (Pc 3) cell line. A green therapeutic approach of molecular mechanism inhibition.

Among the diverse nanomaterials, polymer-based nanocomposites are gained more attention due to their high efficacy, target biological activities, biodegradability and biocompatibility-gum acacia (GA) - a polymer obtained from acacia trees-is considering the multifunctional nanocomposite synthesis. Distinctive Physico-chemical and biocompatibility properties of gum acacia are utilised to prepare a highly stable, biologically active, eco-friendly Nanocomposite. In this current investigation, gum acacia - poly ethylene glycol grafted iron oxide nanocomposite (GA-PEG-IONC) was synthesised by in situ green science principles. The synthesised Nanocomposite was evaluated against the molecular mechanism of urinary tract pathogenic bacterial strains and prostate cancer cells (Pc 3). Nanocomposite prepared in this examination exhibited notable structural, functional stability with nanoarchitecture which was affirmed by Fourier transform infrared spectroscopy (FTIR), electron microscopic studies, atomic force microscopy (AFM), vibrating sample magnetometric analysis (VSM) and X-ray diffraction (XRD), Synthesised Nanocomposite brought about notable antibacterial activity against urinary tract pathogenic strains by recording potential inhibitory effect on the expression of Las R gene. Inhibition of Las R gene expression reduced notable effect on biofilm development. Anticancer activity against prostate cancer cells (Pc3) was investigated by measurement of HOXB13 gene expression level. Inhibition of HOXB13 gene expression by the IONC brought about structural, functional changes. HOXB13 gene expression inhibition reveals a remarkable cytotoxic effect by recording decreased cell viability. Morphometric analysis by phase-contrast and DAPI fluorescence staining demonstrates that the Nanocomposite prompted cell morphology anomalies or apoptotic changes. Nanocomposite treatment brought about a good sign of Apoptosis by recording enhanced caspase 3 and 9 activities, DNA fragmentation and elevated reactive oxygen species generation (ROS). Hemocompatibility studies were carried out to determine the biocompatibility of the Nanocomposite. Spectrophotometric estimation of plasma haemoglobin, microscopic examination of whole blood cells shows the Nanocomposite was not inciting any indication of toxicity. These findings infer that IONC synthesised in the present study is the promising contender for a broad scope of biomedical applications, especially as an antibacterial and anticancer agent.

Insecticidal fungal metabolites fabricated chitosan nanocomposite (IM-CNC) preparation for the enhanced larvicidal activity - An effective strategy for green pesticide against economic important insect pests.

In the present study, enhanced pesticidal activity and biocompatibility of chitosan nanocomposite prepared with biocompatible polymer chitosan - insecticidal metabolites derived from potential fungal biopesticidal agent Nomuraea rileyi were studied. Insecticidal metabolites were isolated from the culture filtrate of fungal strain grown in sabouraud maltose yeast extract broth (SMYB) and the collected filtrate was extracted with ethyl acetate followed by purification using G-60 silica gel column. Chitosan nanocomposite was prepared with metabolites thus acquired by ionic gelation method. Synthesized nanocomposite was found to have high stability, uniformly dispersed particles with high loading and entrapment efficiency. Insecticidal activity was studied by determination of cumulative mortality against larval instars of Spodoptera litura and changes in biochemical composition of midgut, hemolymph macromolecules which revealed that the nanocomposite was effective against all the larval stages in terms of high mortality, drastic reduction of midgut and hemolymph macromolecules biochemical composition. Biocompatibility of nanocomposite was carried out by evaluation of developmental toxicity against zebrafish and in vitro hemolysis with peripheral blood cells. Chitosan nanocomposite treatment was not induced any toxic effect on the developmental stages of zebra fish. Hemolysis was also not recorded in the nanocomposite treatment. The observed results imply that insecticidal metabolites fabricated chitosan nanocomposite prepared in our present system is a promising candidates for pest control against economically important insect pests without affecting non-target organisms.