RESUMO
OBJECTIVE： To establish the quality standard for Kazakhstan medicine Peganum harmala. METHODS： Ten batches of P. harmala collected in Xinjiang Kazakh region were selected as research objects to investigate their characteristics. Qualitative identification of harmaline and harmine was conducted by TLC. The contents of water， total ash， acid-insoluble ash and ethanol extract were tested according to Chinese Pharmacopoeia（2015 edition）. The contents of harmaline and harmine were determined by HPLC. The determination was performed on X-bridge C18 column（250 mm×4.6 mm，5 μm） with mobile phase consisted of acetonitrile-ammonium acetate buffer （adjusted to 6 with glacial acetic acid， gradient elution） at the flow rate of 1 mL/min. The detection wavelength was set at 267 nm， and column temperature was 25 ℃. The sample size was 5 μL. RESULTS： TLC identification results showed that 10 batches of medicinal material showed clear spots at the same position as harmaline and harmine reference substances. Water， total ash， acid-insoluble ash should not be more than 12％，22％，2％， respectively； ethanol extract must not be less than 16％. HPLC results showed that the linear ranges of harmaline and harmine were 15.22-301.40，15.09-301.80 μg/mL； RSDs of precision， reproducibility and stability tests were all lower than 4％； average recoveries were 100.22％ and 100.94％（all RSD＜2％）. The determination results showed that the content of total alkaloids （harmaline and harmine） should not be less than 6.5 mg/g. CONCLUSIONS： Based on the original standard， test items are added in this study. TLC method is established to identify harmaline and harmine. HPLC method is established to determine their contents. Established quality standard can be used for comprehensive quality control of P. harmala from Xinjiang Kazakh region.
RESUMO
Objective: To investigate the content of total carbonates, total hydroxides and mineral elements in a Kazakh herbal medicine Sahar( an alkaline substance obtained from plant ashes). Methods: Total carbonates and total hydroxides were quantified by using the method which described in GB / T 2306-2008[National standard chemical reagents( potassium carbonate) ]. Besides, The contents of ten mineral elements( potassium, sodium, calcium, magnesium, iron, zinc, manganese, copper,selenium and aluminium) were also measured by using the methods which described in national standards such as GB / T 5009. 91-2003( Determination of potassium and sodium in foods),GB / T 5009. 92-2003( Determination of calcium in foods),GB / T 5009. 90-2003( Determination of iron, magnesium and manganese in foods),GB / T 5009. 14-2003( Determination of zinc in foods),GB / T 5009. 13-2003( Determination of copper in foods),GB / T5009. 93-2003( Determination of selenium in foods),GB / T 23374-2009( Determination of aluminium in foods-Inductively coupled plasma mass spectrometry),respectively. Results: The experimental datas indicated that the average contents of total hydroxides and total carbonates in Sahar from Haloxylon persicum were 23. 19% and 29. 83%,whereas those in Sahar from Chenopodium glaucum were 24. 42%and 43. 30%,respectively. Qualitative analysis revealed the presence of iron and magnesium. Quantitative analysis showed that in both Sahars the sodium and potassium were the most abundant, and calcium, iron and aluminum were followed by. Conclusion: The results will provide scientific basis for the establishment of quality standard for Sahar.
