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1.
Heliyon ; 10(2): e24403, 2024 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-38304780

RESUMO

The HT-29 cell line, derived from human colon cancer, is valuable for biological and cancer research applications. Early detection is crucial for improving the chances of survival, and researchers are introducing new techniques for accurate cancer diagnosis. This study introduces an efficient deep learning-based method for detecting and counting colorectal cancer cells (HT-29). The colorectal cancer cell line was procured from a company. Further, the cancer cells were cultured, and a transwell experiment was conducted in the lab to collect the dataset of colorectal cancer cell images via fluorescence microscopy. Of the 566 images, 80 % were allocated to the training set, and the remaining 20 % were assigned to the testing set. The HT-29 cell detection and counting in medical images is performed by integrating YOLOv2, ResNet-50, and ResNet-18 architectures. The accuracy achieved by ResNet-18 is 98.70 % and ResNet-50 is 96.66 %. The study achieves its primary objective by focusing on detecting and quantifying congested and overlapping colorectal cancer cells within the images. This innovative work constitutes a significant development in overlapping cancer cell detection and counting, paving the way for novel advancements and opening new avenues for research and clinical applications. Researchers can extend the study by exploring variations in ResNet and YOLO architectures to optimize object detection performance. Further investigation into real-time deployment strategies will enhance the practical applicability of these models.

2.
Comput Intell Neurosci ; 2022: 5054641, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36268157

RESUMO

With the emergence of the Internet of Things (IoT), investigation of different diseases in healthcare improved, and cloud computing helped to centralize the data and to access patient records throughout the world. In this way, the electrocardiogram (ECG) is used to diagnose heart diseases or abnormalities. The machine learning techniques have been used previously but are feature-based and not as accurate as transfer learning; the proposed development and validation of embedded device prove ECG arrhythmia by using the transfer learning (DVEEA-TL) model. This model is the combination of hardware, software, and two datasets that are augmented and fused and further finds the accuracy results in high proportion as compared to the previous work and research. In the proposed model, a new dataset is made by the combination of the Kaggle dataset and the other, which is made by taking the real-time healthy and unhealthy datasets, and later, the AlexNet transfer learning approach is applied to get a more accurate reading in terms of ECG signals. In this proposed research, the DVEEA-TL model diagnoses the heart abnormality in respect of accuracy during the training and validation stages as 99.9% and 99.8%, respectively, which is the best and more reliable approach as compared to the previous research in this field.


Assuntos
Arritmias Cardíacas , Eletrocardiografia , Humanos , Eletrocardiografia/métodos , Arritmias Cardíacas/diagnóstico , Computação em Nuvem , Aprendizado de Máquina , Software
3.
Comput Intell Neurosci ; 2022: 6852845, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35958748

RESUMO

According to the World Health Organization (WHO) report, heart disease is spreading throughout the world very rapidly and the situation is becoming alarming in people aged 40 or above (Xu, 2020). Different methods and procedures are adopted to detect and diagnose heart abnormalities. Data scientists are working on finding the different methods with the required accuracy (Strodthoff et al., 2021). Electrocardiogram (ECG) is the procedure to find the heart condition in the waveform. For ages, the machine learning techniques, which are feature based, played a vital role in the medical sciences and centralized the data in cloud computing and having access throughout the world. Furthermore, deep learning or transfer learning widens the vision and introduces different transfer learning methods to ensure accuracy and time management to detect the ECG in a better way in comparison to the previous and machine learning methods. Hence, it is said that transfer learning has turned world research into more appropriate and innovative research. Here, the proposed comparison and accuracy analysis of different transfer learning methods by using ECG classification for detecting ECG Arrhythmia (CAA-TL). The CAA-TL model has the multiclassification of the ECG dataset, which has been taken from Kaggle. Some of the healthy and unhealthy datasets have been taken in real-time, augmented, and fused with the Kaggle dataset, i.e., Massachusetts Institute of Technology-Beth Israel Hospital (MIT-BIH dataset). The CAA-TL worked on the accuracy of heart problem detection by using different methods like ResNet50, AlexNet, and SqueezeNet. All three deep learning methods showed remarkable accuracy, which is improved from the previous research. The comparison of different deep learning approaches with respect to layers widens the research and gives the more clarity and accuracy and at the same time finds it time-consuming while working with multiclassification with massive dataset of ECG. The implementation of the proposed method showed an accuracy of 98.8%, 90.08%, and 91% for AlexNet, SqueezeNet, and ResNet50, respectively.


Assuntos
Aprendizado Profundo , Arritmias Cardíacas/diagnóstico , Computação em Nuvem , Eletrocardiografia/métodos , Humanos , Aprendizado de Máquina
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