Grandmaternal stress during pregnancy and DNA methylation of the third generation: an epigenome-wide association study.

Stress during pregnancy may impact subsequent generations, which is demonstrated by an increased susceptibility to childhood and adulthood health problems in the children and grandchildren. Although the importance of the prenatal environment is well reported with regards to future physical and emotional outcomes, little is known about the molecular mechanisms that mediate the long-term consequences of early stress across generations. Recent studies have identified DNA methylation as a possible mediator of the impact of prenatal stress in the offspring. Whether psychosocial stress during pregnancy also affects DNA methylation of the grandchildren is still not known. In the present study we examined the multigenerational hypothesis, that is, grandmaternal exposure to psychosocial stress during pregnancy affecting DNA methylation of the grandchildren. We determined the genome-wide DNA methylation profile in 121 children (65 females and 56 males) and tested for associations with exposure to grandmaternal interpersonal violence during pregnancy. We observed methylation variations of five CpG sites significantly (FDR<0.05) associated with the grandmother's report of exposure to violence while pregnant with the mothers of the children. The results revealed differential methylation of genes previously shown to be involved in circulatory system processes (FDR<0.05). This study provides support for DNA methylation as a biological mechanism involved in the transmission of stress across generations and motivates further investigations to examine prenatal-dependent DNA methylation as a potential biomarker for health problems.

Behavioural/emotional problems in Brazilian children: findings from parents' reports on the Child Behavior Checklist.

Background. To compare Child Behavior Checklist (CBCL) findings for a large Brazilian general population sample with those for US children considering: (a) mean problem item ratings; (b) fit of the US-derived CBCL 8-syndrome model; (c) scale internal consistency measured by Cronbach's alphas; (d) effects of society, age, gender on CBCL problem scores; and (e) ability to discriminate referred from non-referred children. Methods. Parents of 1228 non-referred 6-to-11-year-olds from three different regions of Brazil and 247 referred 6-to-11-year-olds from one clinic rated their children's behavioural and emotional problems using the CBCL/6-18. Results. Results for mean item ratings and scale internal consistencies were very similar to those found in the US and in Uruguay. Confirmatory factor analysis indicated that Brazilian data showed the best fit to the US 8-syndrome model of all countries studied to date. Gender patterns were comparable to those reported in other societies, but mean problem scores for non-referred Brazilian children were higher than those for US children. Therefore, the CBCL discriminated less well between non-referred and referred children in Brazil than in the US. Conclusions. Overall, our findings replicated those reported in international comparisons of CBCL scores for 31 societies, thereby providing support for the multicultural robustness of the CBCL in Brazil.

[Evaluation of orientation and serological support centers in the Brazilian Northeast]. / Avaliação dos Centros de Orientação e Apoio Sorológico/CTA/Coas da Região Nordeste do Brasil.

This article presents the results of an evaluation of Orientation and Serological Support Centers, or Anonymous HIV Testing Centers, in the Northeast of Brazil. Methodological triangulation was used to evaluate these health programs and services, including qualitative and quantitative methodology and pointing to the insufficiency of single-discipline reasoning to encompass phenomena in social organizations. The article also shows results from this triangulation experience, seeking to combine Social Sciences and Epidemiology. It describes the structure and dynamics of these services, analyzes the relationships, perceptions, and representations of the actors, presents a self-evaluation by the professionals, expounds on some quantitative results, and discusses some limits and problems, as well as proposals to overcome them.

[Teenage law offenders in Rio de Janeiro and institutions that "rehabilitate" them. The perpetuation of negligence]. / Os adolescentes infratores do Rio de Janeiro e as instituições que os "ressocializam". A perpetuação do descaso.

This article presents data from juvenile delinquents in the Rio de Janeiro Minors Court. It is an exploratory study with qualitative and quantitative approaches, conducted in 1994 in three schools (Padre Severino Institute, João Luiz Alves School, and Santos Dumont School) in Greater Metropolitan Rio de Janeiro and responsible for the legal custody of juvenile delinquents. Social and demographic aspects of the adolescents and families are shown, and the daily routines of employees, adolescents, and researchers are reported. This paper stresses the damaging effects on the lives of these youngsters by their families, society, and judiciary and legal custody institutions. It also suggests approaches and positive ways of reorienting the issue.

[Violence in the city of Rio de Janeiro: areas of risk and mortality trends among adolescents 10-19 years of age]. / Violência no Município do Rio de Janeiro: áreas de risco e tendências da mortalidade entre adolescentes de 10 a 19 anos.

This study presents data on general mortality and mortality from external causes (traffic accidents and homicides) among adolescents 10 to 19 years of age in Rio de Janeiro Municipality, Brazil, from 1980 to 1994. The purpose of the study was to describe and put into context mortality from external causes and to identify areas of the Municipality where the risk of these occurrences is highest. The study also sought to identify trends and seasonal patterns of this mortality and to place violence within the ambit of public health through application of the epidemiologic surveillance approach. Information on mortality was extracted from death certificates provided by the State Health Secretariat of Rio de Janeiro. A total of 20,224 death certificates were studied. The populations used as the denominators for determining rates were estimated from the censuses of 1980 and 1991. For purposes of the analysis; the Municipality was divided into eight programmatic areas, each composed of diverse neighboring districts. A time series analysis was done for traffic accidents and homicides. The results showed that external causes were the leading causes of death in the 10-19 age group, and that during the study period deaths from these causes had increased, especially among males. The results also revealed that mortality was heterogeneously distributed in areas of the Municipality that differed socioeconomically. The study points to the need for better integration of the health sector with other sectors and institutions and for a restructuring of the health system with regard to epidemiologic surveillance of violence.

[Morbidity due to violence against children and adolescents in Rio de Janeiro]. / Morbidade por violência em crianças e adolescentes do município do Rio de Janeiro.

An analysis of police records was carried out in the Programatic Areas (Aps) of the city of Rio de Janeiro in 1990. 8000 non-fatal records were investigated, covering children and adolescents from 0 to 19 years old. These violent events were categorized as traffic accidents, physical abuse, robbery/theft and attempt of robbery/theft, sexual abuse, extortion/sequestration, threat, neglect-abandon,drugs use/traffic, and attempt of suicide/homicide. The aim of this paper is to identify the most frequent type of violence in the different Aps. According to the findings, traffic accidents, physical abuse and robbery/theft are the most common violent events affecting, especially boys in 10-19 age group. The APs 1 and 3.3 show the highest rates. The article concludes with difficulties and possibilities of dealing with violence in the health field, especially in the professional training.

[Health and violence in infancy and adolescence]. / Saúde e violência na infância e na adolescência.

This paper deals with the conceptualization and problematization of data on violence against infancy and adolescence in Brazil, varying from mortality data to the most subtle and concealed forms of domestic violence. It suggests the appropriation of such information by the health system and its professionals, aiming at the adequacy of their services and policies.