Vertical flow constructed wetlands for decentralized wastewater treatment in Jordan: Optimization of total nitrogen removal.

The baseline performance of two full-scale vertical flow (VF) constructed wetlands operating in the arid climate of Jordan is presented in this study, within the context of the Jordanian Standards for reuse of treated wastewater. One system was a recirculating VF wetland, and the other was a single-pass two-stage VF wetland. Operational modifications were made to each treatment system, with the aim of improving Total Nitrogen (TN) removal. For the recirculating VF system, attached-growth media was added to the recirculation tank to provide increased surface area for growth of denitrifying bacteria. The modification showed a small but significant improvement in TN removal (8 mg/L less than the baseline phase; p = 0.004). Statistical analysis showed that 30% and 4.5% of the increase in compliance with the TN limits (Class A and Class B/C, respectively) could be attributed to the modification. The two-stage VF wetland was modified with a step-feeding line that introduced carbon-rich raw wastewater to the intermediate pump shaft just upstream of the second-stage filter. The modification also resulted in a small but significant improvement in TN removal (13 mg/L less than the baseline phase; p = 0.005). The increase in compliance with the TN standard due to the modification was estimated at 20% and 22% for Class A and B/C, respectively. The simple operational modifications proved to be effective for improving total nitrogen removal in arid climate VF wetland systems.

Side-by-side comparison of 15 pilot-scale conventional and intensified subsurface flow wetlands for treatment of domestic wastewater.

This study reports a systematic assessment of treatment efficacy for 15 pilot-scale subsurface flow constructed wetlands of different designs for CBOD5, TSS, TOC, TN, NH4-N, NO3-N, NO2-N, and E. coli over the course of one year in an outdoor study to evaluate the effects of design and plants. The systems consisted of a range of designs: horizontal flow (HF) with 50 and 25 cm depth, unsaturated vertical flow (VF) with sand or fine gravel, and intensified systems (horizontal and saturated vertical flow with aeration, and reciprocating fill and drain). Each system was built in duplicate: one was planted with Phragmites and one was left unplanted (with the exception of the reciprocating system, of which there was only one and it was unplanted). All systems were fed with the same primary-treated domestic wastewater. Effluent concentrations, areal and volumetric mass removal rates, and percent mass removal for the 15 systems are discussed. HF wetlands removed CBOD5, TSS, TN, NH4-N and E. coli by 73-83%, 93-95%, 17-41%, 0-27% and 1.5 log units, respectively. Unsaturated VF and aerated VF wetlands removed CBOD5, TSS, TN, NH4-N and E. coli by 69-99%, 76-99%, 17-40%, 69-99% and 0.9-2.4 log units, respectively. The aerated HF and reciprocating systems removed CBOD5, TSS, TN, NH4-N and E. coli by 99%, 99%, 43-70%, 94-99% and 3.0-3.8 log units, respectively. The aerated HF and reciprocating systems achieved the highest TN removal rate of all of the designs. Design complexity clearly enhanced treatment efficacy (HF < VF < Intensified, p < 0.001) during the first two years of plant growth while the presence of plants had minor effects on TN and NH4-N removal in the shallow HF design only.

Resilience of carbon and nitrogen removal due to aeration interruption in aerated treatment wetlands.

Treatment wetlands have long been used for domestic and industrial wastewater treatment. In recent decades, treatment wetland technology has evolved and now includes intensified designs such as aerated treatment wetlands. Aerated treatment wetlands are particularly dependent on aeration, which requires reliable air pumps and, in most cases, electricity. Whether aerated treatment wetlands are resilient to disturbances such as an aeration interruption is currently not well known. In order to investigate this knowledge gap, we carried out a pilot-scale experiment on one aerated horizontal flow wetland and one aerated vertical flow wetland under warm (Twater>17°C) and cold (Twater<10°C) weather conditions. Both wetlands were monitored before, during and after an aeration interruption of 6d by taking grab samples of the influent and effluent, as well as pore water. The resilience of organic carbon and nitrogen removal processes in the aerated treatment wetlands depended on system design (horizontal or vertical flow) and water temperature. Organic carbon and nitrogen removal for both systems severely deteriorated after 4-5d of aeration interruption, resulting in effluent water quality similar to that expected from a conventional horizontal sub-surface flow treatment wetland. Both experimental aerated treatment wetlands recovered their initial treatment performance within 3-4d at Twater>17°C (warm weather) and within 6-8d (horizontal flow system) and 4-5d (vertical flow system) at Twater<10°C (cold weather). In the vertical flow system, DOC, DN and NH4-N removal were less affected by low water temperatures, however, the decrease of DN removal in the vertical flow aerated wetland at Twater>17°C was twice as high as in the horizontal flow aerated wetland. The quick recovery of treatment performance highlights the benefits of aerated treatment wetlands as resilient wastewater treatment technologies.

Community-Level Physiological Profiling of Microbial Communities in Constructed Wetlands: Effects of Sample Preparation.

Community-level physiological profiling (CLPP) using BIOLOG® EcoPlates™ has become a popular method for characterizing and comparing the functional diversity, functional potential, and metabolic activity of heterotrophic microbial communities. The method was originally developed for profiling soil communities; however, its usage has expanded into the fields of ecotoxicology, agronomy, and the monitoring and profiling of microbial communities in various wastewater treatment systems, including constructed wetlands for water pollution control. When performing CLPP on aqueous samples from constructed wetlands, a wide variety of sample characteristics can be encountered and challenges may arise due to excessive solids, color, or turbidity. The aim of this study was to investigate the impacts of different sample preparation methods on CLPP performed on a variety of aqueous samples covering a broad range of physical and chemical characteristics. The results show that using filter paper, centrifugation, or settling helped clarify samples for subsequent CLPP analysis, however did not do so as effectively as dilution for the darkest samples. Dilution was able to provide suitable clarity for the darkest samples; however, 100-fold dilution significantly affected the carbon source utilization patterns (CSUPs), particularly with samples that were already partially or fully clear. Ten-fold dilution also had some effect on the CSUPs of samples which were originally clear; however, the effect was minimal. Based on these findings, for this specific set of samples, a 10-fold dilution provided a good balance between ease of use, sufficient clarity (for dark samples), and limited effect on CSUPs. The process and findings outlined here can hopefully serve future studies looking to utilize CLPP for functional analysis of microbial communities and also assist in comparing data from studies where different sample preparation methods were utilized.

Hydraulic characterization and optimization of total nitrogen removal in an aerated vertical subsurface flow treatment wetland.

In this study, a side-by-side comparison of two pilot-scale vertical subsurface flow constructed wetlands (6.2 m(2)×0.85 m, q(i)=95 L/m(2) d, τ(n)=3.5 d) handling primary treated domestic sewage was conducted. One system (VA-i) was set to intermittent aeration while the other was aerated continuously (VAp-c). Intermittent aeration was provided to VA-i in an 8 h on/4 h off pattern. The intermittently aerated wetland, VA-i, was observed to have 70% less nitrate nitrogen mass outflow than the continuously aerated wetland, VAp-c. Intermittent aeration was shown to increase treatment performance for TN while saving 33% of running energy cost for aeration. Parallel tracer experiments in the two wetlands showed hydraulic characteristics similar to one Continuously Stirred Tank Reactor (CSTR). Intermittent aeration did not significantly affect the hydraulic functioning of the system. Hydraulic efficiencies were 78% for VAp-c and 76% for VA-i.