RESUMO
The effects and mechanism of action of prostaglandin E2 (PGE2) on the ovine cervix are largely unknown in the luteal phase. In these studies we have shown that low levels of EP3-receptor (EP3R) mRNA are present in the ovine cervix and that the PGE2 induces activation of polymorphonuclear leukocytes in the ovine cervix on day 6 of the oestrous cycle. It is possible, therefore, that PGE2 acts on the ovine cervix through coupling to EP3 receptors.
Assuntos
Colo do Útero/fisiologia , Dinoprostona/farmacologia , Receptores de Prostaglandina E/biossíntese , Receptores de Prostaglandina E/genética , Transcrição Gênica , Animais , Sequência de Bases , Colo do Útero/efeitos dos fármacos , Corpo Lúteo/fisiologia , DNA Complementar , Estro , Feminino , Humanos , Reação em Cadeia da Polimerase , Gravidez , RNA Mensageiro/biossíntese , Receptores de Prostaglandina E Subtipo EP3 , Alinhamento de Sequência , Homologia de Sequência do Ácido Nucleico , OvinosAssuntos
Alérgenos/efeitos adversos , Anisakis/imunologia , Antígenos de Helmintos/efeitos adversos , Manipulação de Alimentos , Parasitologia de Alimentos , Hipersensibilidade Imediata/etiologia , Animais , Decapodiformes/parasitologia , Peixes/parasitologia , Congelamento , Temperatura Alta , Humanos , Fatores de RiscoRESUMO
The response of the ovine corpus luteum of pregnancy to the luteolytic effect of prostaglandin F2 alpha (dinoprost) and its analogue, cloprostenol, was tested in both superovulated and untreated ewes by monitoring plasma progesterone concentrations at the time of 'material recognition' and beyond (days 13 to 30). On days 13, 16 and 28, the majority of the superovulated ewes were refractory to 10 mg of dinoprost. The luteolytic efficacy of 250 micrograms of cloprostenol was compared with 10 mg of dinoprost on day 20 of pregnancy in superovulated and untreated animals and the two prostaglandins were also compared on days 26 and 30 in animals previously unresponsive to dinoprost. Generally, cloprostenol was more effective than dinoprost because there was less refractoriness on day 20 and no refractoriness on days 26 and 30 to this prostaglandin. No difference in the sensitivity to this drug was found between the superovulated and untreated groups on days 20 and 26.
