Pre-Partum Supplementation with Polyunsaturated Fatty Acids on Colostrum Characteristics and Lamb Immunity and Behavior after a Mild Post-Weaning Aversive Handling Period.

We studied the effect of pre-natal supplementation with n-3 α-linolenic acid (ALA) combined with a tannin-rich forage on colostrum composition and immunological quality and whether these changes had advantageous effects on lambs' survival and stress reaction to a post-weaning stressor. Forty-eight Latxa ewes were fed during the last five weeks of pregnancy with two experimental diets: a control diet based on a neutral concentrate and forage (tall fescue hay; CO-FES), and a supplemented diet based on polyunsaturated (PUFA)-rich concentrate and tanniferous forage (sainfoin; ALA-SAIN). After parturition, twenty ewes had their blood and colostrum sampled, and their lambs were monitored until post-weaning. Lambs were afterwards subjected to (i) an aversive handling period (AHP) followed by a behavioral assessment and (ii) inflammatory and lymphocyte proliferation challenge. Feeding ALA-SAIN resulted in changes in colostrum fatty acid composition, specifically higher α-linoleic acid (p < 0.001), conjugate linoleic acid (p = 0.005), vaccenic acid (p = 0.006) and long-chain n-3 PUFA (p = 0.004). Pre-partum nutrition did not affect lamb immunoglobulin (Ig) G apparent efficacy absorption, but circulating IgG tended to be higher (p = 0.054) in ALA-SAIN lambs. ALA-SAIN lambs interacted more frequently with other lambs (p = 0.002), whereas ALA-SAIN females spent more time closer to other lambs (p < 0.001). Plasma cortisol was higher (p = 0.047) and plasma interleukin (IL)-2 lower (p = 0.003) in CO-FES lambs. This research highlights the importance of prenatal nutrition on the immune system stimulation and lambs' behavior as a strategy to improve lambs' health and welfare during early life.

The value of a retrospective analysis of slaughter records for the welfare of broiler chickens.

The effects of transport risk factors on deaths on arrival (DOAs; %) and carcass rejections (%) on broiler chickens transported to a slaughterhouse in Southern Spain were assessed using information routinely collected at the slaughterhouse. A total of 2,284 flocks and 10,198,663 broiler chickens, transported in 2,103 commercial transports from 217 different farms to a single slaughterhouse, were studied using the information of veterinary service and slaughter records. Studied risk factors were transport condition at arrival score (good/bad) and plumage condition score (good/bad) at arrival at the slaughterhouse, mixing loads (yes/no), transport duration (min), and season of the year (spring/summer/autumn/winter). Generalized linear mixed models were used to determine the effects of risk factors and their interactions on DOA and carcass rejections. The interactive nature of risk factors affecting both variables was revealed. Mixing loads interacted with transport duration (P < 0.0001), amplifying the negative consequences of long transports on DOA. Mixing loads also interacted with transport conditions at arrival (P = 0.0005), with the impact of bad transport conditions at arrival being particularly negative in the case of mixed loads. These facts raise questions about the suitability of mixing loads both from the animal welfare and economic standpoints. Transport duration interacted with season (P = 0.0003), with the detrimental effect of long distances on DOA being particularly evident during summer and winter, which highlights the need for alternative management measures when hot or cold temperatures are foreseen during transport. Long transports also increased carcass rejections (P < 0.0001). Mixing loads and bad plumage condition exacerbated the overall, detrimental effect of autumn transports on DOA (P = 0.0084 and P = 0.0009, respectively) with respect to summer transports. Similar results were obtained for the interactions between mixing loads and transport season (P = 0.0043) and transport conditions at arrival and transport season (P = 0.0014) on carcass rejections. Overall, results highlight the value of slaughter records to produce information useful to reduce the impact of transport risk factors, improve broiler chicken welfare, and improve slaughterhouse economic results.

Broiler Chickens On-Farm Welfare Assessment: Estimating the Robustness of the Transect Sampling Method.

Assessing commercial broiler chickens' welfare usually comes at the cost of reduced precision due to the large flock sizes and required time commitments. The transect method for on-farm welfare assessment is conducted by walking within delimited paths between feeder and drinker lines within the commercial house, referred to as transects. This non-invasive method is conducted by detecting birds with signs of impaired welfare indicators, which include leg problems, sickness, body wounds, and feather dirtiness. The transect method has been validated for commercial turkey flocks but not for broiler chickens due to the large flock sizes. The aim of this study was to evaluate the robustness of the transect method in broiler chicken flocks through a capture-recapture approach of a known subpopulation of 80 birds. Groups of 10 chickens were captured and individually marked in eight locations of the house. Two observers collected the number and position of the detected marked birds while walking along non-adjacent transects (four samplings/house/day) during the two following days. Detection and repetition rates per house, and within transects, were calculated, as well as the effects of flock density, transect number/house (six vs. eight), and sampling time (morning vs. afternoon). The number of traveled transects was calculated for birds detected more than once, and the population random distribution was tested by comparing the number of observed and expected birds/transect. Results showed more than 64% of detection rate with a repetition rate/house sampling of 24% and per transect of 1.66%. Higher repetition rates in six-transect houses and during morning samplings were detected. The number of traveled transects was higher in eight-transect houses and from birds first detected at walls, indicating longer traveled distances in wider houses. In addition, bootstrapping techniques were used to calculate the optimal sampling effort. Our findings indicate that the lowest repetition rates and optimal sampling can be achieved by assessing two transects, being one wall and one central, separated by three transects in between. Such sampling procedure would provide robust results for welfare assessment of commercial broiler chicken flocks.

On-farm broiler chicken welfare assessment using transect sampling reflects environmental inputs and production outcomes.

To evaluate the utility of transect sampling for assessing animal welfare in large chicken flocks, we quantified relationships between environmental inputs, welfare problems detected using transect sampling, and production outcomes. We hypothesised that environmental inputs including environmental complexity (i.e. number of environmental enrichment types provided), space allowance, underfloor heating (presence or absence), and photoperiod regimen (18 h continuous vs 16 h intermittent) would correspond to variations in welfare assessment findings, which would predict production outcomes. We conducted on-farm welfare assessment of Norwegian broiler flocks at approximately 28 days of age. We sampled four transects (rows between feeder and drinker lines) per flock to determine litter quality and the proportions of chickens with compromised welfare as indicated by visual signs of walking difficulties, illness, skin wounds and small bird size. Production outcome measures included mortality, reasons for carcass rejection at slaughter, footpad dermatitis, growth rate, feed conversion and an integrated production index. Greater environmental complexity was associated with a reduction in skin wounds and total welfare problems on the farm, lower mortality, fewer rejections due to wounds and underweight birds, and fewer rejections overall. Higher space allowances within levels of environmental complexity were associated with fewer walking difficulties and welfare problems overall, a reduction in rejections due to wounds, and a higher growth rate and production index. Underfloor heating was associated with a reduction in rejections due to leg deformity, and intermittent light was associated with lower illness and skin wound rates on the farm, and lower mortality. Furthermore, fewer welfare problems and better litter quality on the farm were associated with fewer carcass rejections at slaughter. Thus, data from transect sampling varied with environmental inputs and production outcomes, supporting the validity of transect sampling for practical, animal-based on-farm welfare assessment.

The Impact of Group Size on Welfare Indicators of Ewes during Pregnancy.

Group size (GS) and space allowance have major implications for the welfare of production species, however their effects are often confounded. In a previous study we investigated the impact of varying space allowance at constant GS. In the present work we report the consequences of varying GS on pregnant ewes while controlling space allowance. We housed ewes at 6 (GS6) or 12 ewes/enclosure (GS12), while controlling space allowance to 1.5 m2/ewe (3 enclosures/treatment), and necessarily varying enclosure size. Therefore, when indicating GS effects we implicitly reflect a confounding effect with that of enclosure size. Movement, use of space, behaviour, serum cortisol concentration and body condition score (BCS) were collected during the last 12 gestation weeks. Movement, use of space, and behaviour were collected every other week, during 2 days/week, using 10 minute continuous scan samplings. Blood was collected during weeks 10, 13, 17, and 21 of gestation, and BCS during weeks 15 and 21. Data were analysed using repeated measures, generalized linear mixed models, with GS, week, and their interaction as fixed effects, and enclosure as random effect. GS mainly affected movement and use of space. GS12 ewes walked longer distances using longer steps (P<0.001). An interaction GS by week was observed for angular dispersion (P<0.0001), which was smaller for GS12 from week 10 onwards. Initial restlessness levels were lower for GS12, as shown by the reduced frequency of location changes (P<0.0001). Furthest and mean neighbour distances increased with GS (P<0.0001). The effect of GS on behaviour was only evident for eating behaviour as an interaction with gestation week (P<0.05). Changes in behaviour, movement and use of space along the study indicated an activity peak during weeks 3 to 5. Cortisol changes during gestation (P<0.01) also reflected this activity peak, while BCS (P<0.001) reflected normal physical condition changes during pregnancy. Although the separate effects of GS and enclosure size cannot be disentangled, we conclude that if enough space/ewe is given during gestation, larger GS will result in larger effective space, and no major implications for the welfare of ewes should be expected as GS increases. Ewes will adapt their movement patterns and use of space to enclosure size, and no further behavioural, physiological and physical consequences should be expected.

Technology and Poultry Welfare.

Consideration of animal welfare is essential to address the consumers' demands and for the long term sustainability of commercial poultry. However, assessing welfare in large poultry flocks, to be able to detect potential welfare risks and to control or minimize its impact is difficult. Current developments in technology and mathematical modelling open new possibilities for real-time automatic monitoring of animal welfare and health. New technological innovations potentially adaptable to commercial poultry are appearing, although their practical implementation is still being defined. In this paper, we review the latest technological developments with potential to be applied to poultry welfare, especially for broiler chickens and laying hens. Some of the examples that are presented and discussed include the following: sensors for farm environmental monitoring, movement, or physiological parameters; imaging technologies such as optical flow to detect gait problems and feather pecking; infrared technologies to evaluate birds' thermoregulatory features and metabolism changes, that may be indicative of welfare, health and management problems. All these technologies have the potential to be implemented at the commercial level to improve birds' welfare and to optimize flock management, therefore, improving the efficiency of the system in terms of use of resources and, thus, long term sustainability.

Space availability in confined sheep during pregnancy, effects in movement patterns and use of space.

Space availability is essential to grant the welfare of animals. To determine the effect of space availability on movement and space use in pregnant ewes (Ovis aries), 54 individuals were studied during the last 11 weeks of gestation. Three treatments were tested (1, 2, and 3 m2/ewe; 6 ewes/group). Ewes' positions were collected for 15 minutes using continuous scan samplings two days/week. Total and net distance, net/total distance ratio, maximum and minimum step length, movement activity, angular dispersion, nearest, furthest and mean neighbour distance, peripheral location ratio, and corrected peripheral location ratio were calculated. Restriction in space availability resulted in smaller total travelled distance, net to total distance ratio, maximum step length, and angular dispersion but higher movement activity at 1 m2/ewe as compared to 2 and 3 m2/ewe (P<0.01). On the other hand, nearest and furthest neighbour distances increased from 1 to 3 m2/ewe (P<0.001). Largest total distance, maximum and minimum step length, and movement activity, as well as lowest net/total distance ratio and angular dispersion were observed during the first weeks (P<0.05) while inter-individual distances increased through gestation. Results indicate that movement patterns and space use in ewes were clearly restricted by limitations of space availability to 1 m2/ewe. This reflected in shorter, more sinuous trajectories composed of shorter steps, lower inter-individual distances and higher movement activity potentially linked with higher restlessness levels. On the contrary, differences between 2 and 3 m2/ewe, for most variables indicate that increasing space availability from 2 to 3 m2/ewe would appear to have limited benefits, reflected mostly in a further increment in the inter-individual distances among group members. No major variations in spatial requirements were detected through gestation, except for slight increments in inter-individual distances and an initial adaptation period, with ewes being restless and highly motivated to explore their new environment.

The Effect of Steps to Promote Higher Levels of Farm Animal Welfare across the EU. Societal versus Animal Scientists' Perceptions of Animal Welfare.

Information about animal welfare standards and initiatives from eight European countries was collected, grouped, and compared to EU welfare standards to detect those aspects beyond minimum welfare levels demanded by EU welfare legislation. Literature was reviewed to determine the scientific relevance of standards and initiatives, and those aspects going beyond minimum EU standards. Standards and initiatives were assessed to determine their strengths and weaknesses regarding animal welfare. Attitudes of stakeholders in the improvement of animal welfare were determined through a Policy Delphi exercise. Social perception of animal welfare, economic implications of upraising welfare levels, and differences between countries were considered. Literature review revealed that on-farm space allowance, climate control, and environmental enrichment are relevant for all animal categories. Experts' assessment revealed that on-farm prevention of thermal stress, air quality, and races and passageways' design were not sufficiently included. Stakeholders considered that housing conditions are particularly relevant regarding animal welfare, and that animal-based and farm-level indicators are fundamental to monitor the progress of animal welfare. The most notable differences between what society offers and what farm animals are likely to need are related to transportation and space availability, with economic constraints being the most plausible explanation.