1.
Proc Natl Acad Sci U S A
; 105(51): 20404-9, 2008 Dec 23.
Artigo
em Inglês
| MEDLINE
| ID: mdl-19073939
RESUMO
We previously reported assembly and cloning of the synthetic Mycoplasma genitalium JCVI-1.0 genome in the yeast Saccharomyces cerevisiae by recombination of six overlapping DNA fragments to produce a 592-kb circle. Here we extend this approach by demonstrating assembly of the synthetic genome from 25 overlapping fragments in a single step. The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments.