Prediction and Diagnosis of Respiratory Disease by Combining Convolutional Neural Network and Bi-directional Long Short-Term Memory Methods.

Objective: Based on the respiratory disease big data platform in southern Xinjiang, we established a model that predicted and diagnosed chronic obstructive pulmonary disease, bronchiectasis, pulmonary embolism and pulmonary tuberculosis, and provided assistance for primary physicians. Methods: The method combined convolutional neural network (CNN) and long-short-term memory network (LSTM) for prediction and diagnosis of respiratory diseases. We collected the medical records of inpatients in the respiratory department, including: chief complaint, history of present illness, and chest computed tomography. Pre-processing of clinical records with "jieba" word segmentation module, and the Bidirectional Encoder Representation from Transformers (BERT) model was used to perform word vectorization on the text. The partial and total information of the fused feature set was encoded by convolutional layers, while LSTM layers decoded the encoded information. Results: The precisions of traditional machine-learning, deep-learning methods and our proposed method were 0.6, 0.81, 0.89, and F1 scores were 0.6, 0.81, 0.88, respectively. Conclusion: Compared with traditional machine learning and deep-learning methods that our proposed method had a significantly higher performance, and provided precise identification of respiratory disease.

[Meta-analysis of the association between PPARγ2 gene Pro12Ala polymorphism and obesity in Chinese population].

OBJECTIVE: To explore the relationship between the peroxisome proliferator-activated receptor γ(PPARγ) 2 gene Pro12 Ala polymorphism and the risk of obesity in the Chinese population. METHODS: Take "peroxisome proliferator activated receptor gamma2", "PPAR gamma2", "pparg2", "peroxisome proliferator-activated receptor gamma2", "obesity", "Pro12 Ala", "gene polymorphism", "Chinese" as searchable words, Chinese people were selected as study subjects from Chinese databases such as CNKI, Wanfang Database, VIP and foreign language databases such as PubMed, Embase, Cochrane Library and Web of Science, which were published from the establishment of the database until December 31, 2019. RevMan 5. 3 software was used for Meta-analysis of the literature with literature score ≥ 5 points. RESULTS: A total of 12 articles were included, including 2233 cases and 1795 healthy controls. Meta-analysis showed that:(1) Dominant gene model: the risk of obesity for those carrying Ala/Ala+Pro/Ala was 1. 86 times that of those carrying Pro/Pro(OR=1. 86, 95%CI 1. 07-3. 21). (2) Additive gene model: the risk of obesity for those carrying Pro/Ala was 1. 79 times that of those carrying Pro/Pro(OR=1. 79, 95% CI 1. 04-3. 08). (3) Allelic model: the risk of obesity in those carrying the allele Ala was 1. 75 times that of those carrying the allele Pro(OR=1. 75, 95% CI 1. 11-2. 78). The subgroup analysis of different regions showed that the combined OR(95% CI) values of the dominant gene model, additive gene model and allelic model in the southern region were 1. 96(1. 12-3. 42), 2. 02(1. 14-3. 56) and 1. 79(1. 06-3. 03), the combined OR(95% CI) values of the dominant gene model, additive gene model and allele model in the northern region were 1. 89(0. 90-3. 97), 1. 77(0. 85-3. 67) and 1. 80(0. 97-3. 36). CONCLUSION: The polymorphic allele Ala or genotype Ala/Ala or Pro/Ala of PPARγ2 gene may increase the risk of obesity in Chinese population. The correlation between the PPARγ2 gene Pro12 Ala polymorphism and the risk of obesity in the southern population is more obvious.

Effects of 2,2',4,4' -tetrabromodiphenyl ether on differentiation of mouse 3T3-L1 cells / 预防医学

Objective@#To investigate the effects of 2, 2', 4, 4'-tetrabromodiphenyl ether ( BDE-47 ) on the differentiation of mouse embryonic fibroblasts-3T3-L1, so as to provide the basis for revealing the mechanism of environmental obesity factors. @*Methods@#The 3T3-L1 cells were divided into five BDE-47 intervention groups ( 25, 18.75, 12.5, 7.5 and 2.5 µmol/L ), a positive control group (1 µmol/L 2, 4-thiazolidinedione) and a negative control group ( 0.1% dimethyl sulfoxide ) for the induction of differentiation. The lipid droplet accumulation in adipocytes was observed by oil red O staining treatment and detection of optical desity ( OD ) on the eighth day of differentiation. Triglyceride ( TG ) content was measured using the histiocyte TG enzymatic assay kit. The mRNA expression of adiponectin and peroxisome proliferator activated receptor-γ ( PPARγ ) was measured by RT-PCR.@*Results@#The positive areas of oil red O staining, OD values, TG content and mRNA expression of adiponectin and PPARγ in 3T3-L1 cells were significantly different among seven groups ( P<0.05 ). The positive areas of oil red O staining and OD values in the BDE-47 groups with different concentrations were higher than those in the negative control group ( P<0.05 ). The 18.75 µmol/L BDE-47 group had higher TG levels than the negative control group ( P<0.05 ). The mRNA expression of PPARγ in the 25, 18.75, 12.5, and 7.5 µmol/L BDE-47 groups and the positive control group was higher than that in the negative control group ( P<0.05 ). The mRNA expression of PPARγ in the 12.5 µmol/L BDE-47 group was higher than that in the 25, 18.75, 7.5, 2.5 µmol/L BDE-47 group and the positive control group ( P<0.05 ). The mRNA expression of adiponectin in the 12.5, 7.5 µmol/L BDE-47 group and the positive control group was higher than that in the negative control group ( P<0.05 ). The mRNA expression of adiponectin in the 12.5 µmol/L BDE-47 group was higher than that in the 25, 18.75, 2.5 µmol/L BDE-47 group ( P<0.05 ). The mRNA expression of PPARγ and adiponectin in the different concentration groups of BDE-47 distributed like inverted "U" shape.@*Conclusion@#BDE-47 can promote the differentiation of 3T3-L1 preadipocytes. Low concentration of BDE-47 may induce adipocyte differentiation by activating PPARγ.

[Restrictive cubic spline analysis of dose-response relationship between maternal polybrominated diphenyl ethers exposure level and neonatal low birth weight in Urumqi].

OBJECTIVE: To explore the dose-response relationship between maternal polybrominated diphenyl ethers(PBDEs) exposure and neonatal low birth weight(LBW) by restrictive cubic spline method. METHODS: Blood samples from 96 full-term primiparas in Urumqi were collected and the concentrations of seven PBDEs(BDE-28, BDE-47, BDE-99, BDE-100, BDE-153, BDE-154, BDE-183) in serum were measured to calculate the total PBDEs concentration(ΣPBDEs). The sociodemographic data of pregnant women and their spouses, prenatal examination, nutritional status during pregnancy, internal and external environmental factors and neonatal birth outcomes were collected to analyze the influencing factors affecting the low birth weight of the newborn and explore the dose-response relationship between the exposure level of maternal PBDEs and the birth weight of the newborn. RESULTS: (1) ΣPBDEs ranged from 144.67 to 32884.70 pg/g lipid weight(LW), and the median concentration was 3930.93 pg/g LW. BDE-153 was the main source of contribution. The median concentration was 41.04%.(2) There was a positive correlation between ΣPBDEs and birth outcomes such as birth length and body weight, both P<0.05.(3) There were significant differences in the distribution of PBDEs, weight gain during pregnancy, birth weight, Apgar score, house decoration before and after pregnancy, number of home appliances and number of carpets in normal birth weight infants and low birth weight infants(P<0.05).(4) Restricted cubic splines showed a non-linear "∩" dose-response relationship between the exposure level of PBDEs during pregnancy and the intensity of birth weight. The relationship between the exposure level of PBDEs during pregnancy and the intensity of LBW showed a weak nonlinear "^" dose-response relationship. The correlation intensity between the level and the normal birth weight showed a non-linear "^" dose response relationship(non-linear test, both P<0.05). CONCLUSION: The blood level of PBDEs in pregnant women in Urumqi is low, but it can affect the birth outcome of newborns. The correlation between the exposure level of PBDEs and birth weight during pregnancy is nonlinear dose-response relationship.