RESUMO
Fermented foods (FFs) have been a part of our diets for millennia and comprise highly diverse products obtained from plants and animals all over the world. Historically, fermentation has been used to preserve food and render certain raw materials edible. As our food systems evolve towards more sustainability, the health benefits of FFs have been increasingly touted. Fermentation generates new/transformed bioactive compounds that may occur in association with probiotic bacteria. The result can be specific, advantageous functional properties. Yet, when considering the body of human studies on the topic, whether observational or experimental, it is rare to come across findings supporting the above assertion. Certainly, results are lacking to confirm the widespread idea that FFs have general health benefits. There are some exceptions, such as in the case of lactose degradation via fermentation in individuals who are lactose intolerant; the impact of select fermented dairy products on insulin sensitivity; or the benefits of alcohol consumption. However, in other situations, the results fail to categorically indicate whether FFs have neutral, beneficial, or detrimental effects on human health. This review tackles this apparent incongruity by showing why it is complex to test the health effects of FFs and what can be done to improve knowledge in this field.
Assuntos
Produtos Fermentados do Leite , Alimentos Fermentados , Probióticos , Animais , Fermentação , Humanos , LactoseRESUMO
AIMS: To characterize fibre degradation, colonization and fermentation, and xylanase activity of two xylanolytic bacteria Bacteroides xylanisolvens XB1A(T) and Roseburia intestinalis XB6B4 from the human colon. METHODS AND RESULTS: The bacteria grew well on all the substrates chosen to represent dietary fibres: wheat and corn bran, pea, cabbage and leek fibres, and also on purified xylans. Roseburia intestinalis colonized the substrates more efficiently than Bact. xylanisolvens. For the two bacteria, 80-99% of the total xylanase activity was associated with the cells whatever the substrate and time of growth. Optimal specific activities of cells were obtained on oat spelt xylan; they were higher than those previously measured for xylanolytic bacteria from the human gut. Roseburia intestinalis produced high molecular mass xylanases (100-70 kDa), while Bact. xylanisolvens produced lower molecular mass enzymes, including a cell-associated xylanase of 37 kDa. CONCLUSIONS: The two bacteria display very high xylanolytic activity on the different substrates. Differences were observed on substrate attachment and enzyme systems, suggesting that the two species occupy different niches within the gut microbiota. SIGNIFICANCE AND IMPACT OF THE STUDY: This study characterizes xylan degradation by two major species of the human intestine.
Assuntos
Bacteroides/enzimologia , Colo/microbiologia , Fibras na Dieta/metabolismo , Fermentação , Bactérias Gram-Positivas/enzimologia , Xilosidases/metabolismo , Bacteroides/crescimento & desenvolvimento , Bacteroides/isolamento & purificação , Metabolismo dos Carboidratos , Bactérias Gram-Positivas/crescimento & desenvolvimento , Bactérias Gram-Positivas/isolamento & purificação , Humanos , Xilanos/metabolismoRESUMO
AIM: To examine the effect of concentrate and yeast additive on the number of cellulolytic bacteria in the rumen of sheep. METHODS AND RESULTS: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens were quantified using real-time PCR (targeting 16S rDNA) in parallel to cellulolytic flora enumeration with cultural techniques. Whatever the conditions tested, R. flavefaciens was slightly more abundant than F. succinogenes, with both species outnumbering R. albus. Before feeding, the shift from hay to hay plus concentrate diet had no effect on rumen pH and on the number of the three specie; while after feeding, the concentrate-supplemented diet induced a decrease (-1 log) of the number of the three species concomitant with the rumen acidification. Overall, the presence of the live yeast resulted in a significant increase (two- to fourfold) of the Ruminococci. CONCLUSION: The use of real-time PCR allowed us to show changes in the number of cellulolytic bacterial species in vivo in response to diet shift and additives that could not be as easily evidenced by classical microbial methods. SIGNIFICANCE AND IMPACT OF THE STUDY: This study contributes to the understanding of the negative impact of readily fermentable carbohydrates on rumen cellulolysis and the beneficial effect of yeast on rumen fermentation.
Assuntos
Ração Animal , Celulose/metabolismo , Bactérias Gram-Positivas/isolamento & purificação , Rúmen/microbiologia , Carneiro Doméstico/microbiologia , Leveduras , Animais , Metabolismo dos Carboidratos , Contagem de Colônia Microbiana , DNA Bacteriano/análise , Suplementos Nutricionais , Fermentação , Fibrobacter/genética , Fibrobacter/isolamento & purificação , Fibrobacter/metabolismo , Bactérias Gram-Positivas/metabolismo , Masculino , Sondas de Oligonucleotídeos/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Rúmen/metabolismo , Ruminococcus/genética , Ruminococcus/isolamento & purificação , Ruminococcus/metabolismo , Carneiro Doméstico/metabolismoRESUMO
The composition of the human cecal microbiota is poorly known because of sampling difficulties. Samples of cecal fluid from eight subjects were collected via an intestinal tube. Feces were also collected. Total anaerobes, facultative anaerobes, bifidobacteria, and Bacteroides were enumerated by culture methods, and the predominant phylogenetic groups were quantified by molecular hybridization using a set of six rRNA-targeted probes. The numbers of strict anaerobes, bifidobacteria, Bacteroides, and members of the Clostridium coccoides group and Clostridium leptum subgroup were lower in the cecum. Facultative anaerobes represented 25% of total bacteria in the cecum versus 1% in the feces.
Assuntos
Bactérias Anaeróbias/classificação , Bactérias Anaeróbias/isolamento & purificação , Ceco/microbiologia , Fezes/microbiologia , Adulto , Bacteroides/classificação , Bacteroides/isolamento & purificação , Bifidobacterium/classificação , Bifidobacterium/isolamento & purificação , Clostridium/classificação , Clostridium/isolamento & purificação , Meios de Cultura , Feminino , Humanos , Masculino , RNA Ribossômico 16S/genéticaRESUMO
The endoglucanase activity of cells and extracellular culture fluid of Fibrobacter succinogenes S85 grown on glucose, cellobiose, soluble polysaccharides (beta-glucan, lichenan) and intact plant polysaccharides, was compared. The specific activity of cells grown on cellulose or forages was 6- to 20-fold higher than that of cells grown on soluble substrates, suggesting an induction of endoglucanases by the insoluble substrates. The ratios of cells to extracellular culture fluid endoglucanase activities measured in cultures grown on sugars or insoluble polysaccharides suggested that the endoglucanases induced by the insoluble polysaccharides remained attached to the cells. The mRNA of all the F. succinogenes glycoside hydrolase genes sequenced so far were then quantified in cells grown on glucose, cellobiose or cellulose. The results show that all these genes were transcribed in growing cells, and that they are all overexpressed in cultures grown on cellulose. Endoglucanase-encoding endB and endA(FS) genes, and xylanase-encoding xynC gene appeared the most expressed genes in growing cells. EGB and ENDA are thus likely to play a major role in cellulose degradation in F. succinogenes.
Assuntos
Proteínas de Bactérias/biossíntese , Celulase/metabolismo , Glicosídeo Hidrolases/biossíntese , Celobiose , Meios de Cultura , Primers do DNA , Regulação Enzimológica da Expressão Gênica , Glucose , Glicosídeo Hidrolases/genética , RNA Mensageiro/análise , TrissacarídeosRESUMO
A beta-1,4-endoglucanase named MI-ENG1, homologous to the family 5 glycoside hydrolases, was previously isolated from the plant parasitic root-knot nematode Meloidogyne incognita. We describe here the detection of the enzyme in the nematode homogenate and secretion and its complete biochemical characterization. This study is the first comparison of the enzymatic properties of an animal glycoside hydrolase with plant and microbial enzymes. MI-ENG1 shares many enzymatic properties with known endoglucanases from plants, free-living or rumen-associated microorganisms and phytopathogens. In spite of the presence of a cellulose-binding domain at the C-terminus, the ability of MI-ENG1 to bind cellulose could not be demonstrated, whatever the experimental conditions used. The biochemical characterization of the enzyme is a first step towards the understanding of the molecular events taking place during the plant-nematode interaction.
Assuntos
Celulase/química , Proteínas de Helminto/química , Tylenchoidea/enzimologia , Sequência de Aminoácidos , Animais , Carboximetilcelulose Sódica/metabolismo , Cátions Bivalentes , Celulase/isolamento & purificação , Celulase/metabolismo , Celulase/fisiologia , Celulose/metabolismo , Quelantes/farmacologia , Fenômenos Químicos , Físico-Química , Ácido Edético/farmacologia , Escherichia coli , Glucanos/metabolismo , Glicosídeo Hidrolases/química , Glicosídeo Hidrolases/classificação , Glicosídeo Hidrolases/metabolismo , Proteínas de Helminto/isolamento & purificação , Proteínas de Helminto/metabolismo , Proteínas de Helminto/fisiologia , Interações Hospedeiro-Parasita , Dados de Sequência Molecular , Raízes de Plantas/parasitologia , Plantas Tóxicas , Ligação Proteica , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/genética , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Especificidade por Substrato , Nicotiana/citologia , Tylenchoidea/fisiologia , Xilanos/metabolismoRESUMO
The enzymatic properties of two endoglucanases from Fibrobacter succinogenes, EGB and EGC, were analysed. EGB and EGC were purified from recombinant Escherichia coli cultures expressing their gene. The failure of purification of EGB by classical techniques led us to produce antipeptide antibodies that allowed immunopurification of the protein from E. coli as well as its detection in F. succinogenes cultures. Synthetic peptides were selected from the predicted primary structure of EGB, linked to bovine serum albumin and used as immunogens to obtain specific antibodies. One of the polyclonal antipeptide antisera was used to purify EGB. EGC was purified by affinity chromatography with Ni-NTA resin. The endo mode of action of the two enzymes on carboxymethyl-cellulose was different. The values of K(m) and V(max) were respectively 13.6 mg/ml and 46 micromol/min mg protein for EGB, and 7 mg/ml and 110 micromol/min mg protein for EGC. The reactivity of the antipeptide and the anti-EGC sera with F. succinogenes proteins of molecular mass different from that of EGB and EGC produced in E. coli suggested post-translational modification of the two enzymes in F. succinogenes cultures. Expression of endB and endC genes in F. succinogenes was confirmed by RT-PCR.