RESUMO
Genetic variation of two Ziziphus spina-christi L. (Sidr) varieties was determined by Random Amplified Polymorphic DNA (RAPD). The activity of antioxidant enzymes (peroxidase and catalase) was also determined for the two varieties (Balady and Pakistani). Moreover, the antibacterial effects of different concentrations of aqueous and ethanolic extracts of leaves of the two varieties of Ziziphus spina-christi L. were assessed against Gram-positive bacteria (Streptococcus pyogenes and Staphylococcus aureus) and Gram-negative (Klebsiella pneumoniae, Proteus mirablis and Escherichia coli). The similarity of RAPD profiles was found to be 85%. Our results suggest that Ziziphus spina-christi is an effective source of antioxidants and its leaf extract is a potential promising alternative antibacterial agent against different pathogenic bacteria.
Assuntos
Variação Genética/genética , Extratos Vegetais/farmacologia , Ziziphus/química , Ziziphus/genética , Ziziphus/metabolismo , Catalase/metabolismo , Testes de Sensibilidade Microbiana/métodos , Peroxidase/metabolismo , Extratos Vegetais/química , Folhas de Planta/química , EspectrofotometriaRESUMO
The karyotypes of 21 accessions of Lathyrus L. belonging to four sections were investigated. Although all the species have a chromosome number of 2n = 14, they could be differentiated by their karyotype formula and quantitative parameters of the karyotypes. Phenetic distance showed that in spite of the differences observed among entitied, they can be grouped in clusters that coincide with the taxonomic sections established by Kupicha and with the life cycle of the species. The section Clymenum can be distinguished by the presence of a subtelocentric pair. From an evolutionary point of view, variation in genome size, however, is congruent with morphological variation as well as with the life cycle.
Assuntos
Cromossomos de Plantas/genética , Evolução Molecular , Cariotipagem , Lathyrus/genética , Dados de Sequência Molecular , FilogeniaRESUMO
The results in the present study emphasize that in Arabidopsis thaliana genome, gamma-GTase encoded by a gene family comprised of four genes. Three of these genes are functionally expressed while the fourth is a pseudogene. The three functional genes express different active protein isoforms that appeared to have different functions. Two genes (GGT1 and GGT2) are structurally similar and expresses proteins with the same molecular weight and both target to the same cellular compartment (plasma membrane). However, both demonstrated different tissue localization, physiological activities and different patterns of response to environmental stress. The majority of GGTI encoded by the first gene was localized in the rosette leaves, It is also expressed throughout the whole plant.
