Assuntos
Talassemia beta/etnologia , Talassemia beta/genética , Alelos , Estudos de Coortes , Consanguinidade , Egito/epidemiologia , Testes Genéticos , Genótipo , Globinas/genética , Heterozigoto , Homozigoto , Humanos , Região do Mediterrâneo/etnologia , Oriente Médio/etnologia , Mutação , Análise de Sequência de DNARESUMO
Previous studies have suggested that intercellular adhesion molecule-1 (ICAM-1; CD54) may be involved in the pathogenesis of asthma. In addition, a soluble form of intercellular adhesion molecule-1 (sICAM-1) has been detected in increased concentrations in the sera from adult patients with certain inflammatory, immune, or malignant diseases. To determine whether bronchial asthma exacerbation in children is associated with increased levels of serum sICAM-1 and to investigate the effect of the severity of exacerbation on these levels, the concentrations of sICAM-1 were measured in sera of 20 healthy control children and 45 asthmatic children (15 with mild, 15 with moderate, and 15 with severe asthma exacerbation) using an immunoenzymatic assay. Assessment of the severity of asthma exacerbation was based on clinical and physiological parameters. The mean (+/- SD) level of serum sICAM-1 for asthmatic children (390.0+/-108.3 ng/ml) was significantly higher than that for healthy (193.2+/-33.95 ng/ml; p = 0.000). We have also found a differential rise of serum sICAM-1 level which correlates well with the severity of asthma exacerbation. The elevated concentrations of serum sICAM-1 in acute bronchial asthma may reflect the extensive inflammatory response occurring in the airways during acute exacerbation of the disease with airway obstruction. The results of this study suggest that serum sICAM-1 is a promising serological marker of the severity of inflammation in bronchial asthma in children and it would not only facilitate staging of inflammation but also allow the monitoring of therapy and intervention.
Assuntos
Asma/sangue , Molécula 1 de Adesão Intercelular/sangue , Criança , Feminino , Humanos , Masculino , Testes de Função Respiratória , Mecânica Respiratória , Índice de Gravidade de Doença , SolubilidadeRESUMO
To study the value of eosinophil cationic protein (ECP) as a serological marker of disease activity in childhood bronchial asthma, ECP levels were measured in 20 healthy control children and 25 asthmatic children, during and 2 weeks after acute exacerbation. The mean serum ECP level of all asthmatic patients, during and after exacerbation, was significantly higher than the control group and was significantly higher during attacks than 2 weeks after their termination. ECP levels were highest in severe attacks, but did not differ between mild and moderate attacks. ECP levels in asthmatic patients 2 weeks after mild and moderate attacks were comparable to normal; after severe attacks levels remained higher than normal. Measurement of serum ECP will be helpful in determining asthma activity and deciding the use of anti-asthma drugs.
Assuntos
Asma/sangue , Proteínas Sanguíneas/metabolismo , Ribonucleases , Doença Aguda , Adolescente , Asma/classificação , Asma/diagnóstico , Asma/imunologia , Biomarcadores/sangue , Gasometria , Estudos de Casos e Controles , Criança , Proteínas Granulares de Eosinófilos , Eosinófilos , Feminino , Humanos , Contagem de Leucócitos , Masculino , Pico do Fluxo Expiratório , Radioimunoensaio , Índice de Gravidade de Doença , Fatores de TempoRESUMO
To study the value of eosinophil cationic protein [ECP] as a serological marker of disease activity in childhood bronchial asthma, ECP levels were measured in 20 healthy control children and 25 asthmatic children, during and 2 weeks after acute exacerbation. The mean serum ECP level of all asthmatic patients, during and after exacerbation, was significantly higher than the control group and was significantly higher during attacks than 2 weeks after their termination. ECP levels were highest in severe attacks, but did not differ between mild and moderate attacks. ECP levels in asthmatic patients 2 weeks after mild and moderate attacks were comparable to normal; after severe attacks levels remained higher than normal. Measurement of serum ECP will be helpful in determining asthma activity and deciding the use of anti-asthma drugs