RESUMO
The glycoprotein hormones lutropin (LH) and thyrotropin and a limited number of additional glycoproteins bear carbohydrate structures terminating with the unique sequence SO(4)-4-GalNAcbeta1,4GlcNAcbeta that has been conserved in the glycoprotein hormones of all vertebrate species. Synthesis of these structures is mediated by a protein-specific beta1,4GalNAc-transferase and a GalNAc-4-sulphotransferase (GalNAc-4-ST1). GalNAc-4-ST1 is a member of a family of sulphotransferases that includes HNK-1 sulphotransferase, chondroitin-4-sulphotransferases-1-3 and dermatan-4-sulphotransferase-1. With the exception of HNK-1-ST, these sulphotransferases add sulphate to the C-4 hydroxy group of either terminal or non-terminal beta1,4-linked GalNAc. GalNAc-4-ST1 is most highly expressed in pituitary, cerebellum and other regions of the brain. The terminal GalNAcSO(4) on LH is recognized by the cysteine-rich domain of the mannose/GalNAc-4-SO(4) receptor located in hepatic endothelial cells. Each cysteine-rich domain binds a single terminal GalNAc-4-SO(4), and the receptor must form non-covalently associated homodimers in order to simultaneously engage two GalNAc-4-SO(4) moieties on separate oligosaccharides with sufficient affinity to form stable complexes. The receptor mediates the clearance of LH from the blood. This clearance, in conjunction with the stimulated release of hormone from dense core granules in pituitary gonadotroph cells, is required to produce the episodic rise and fall in LH levels needed for optimal oestrogen production during the implantation of embryos in the uterus.
Assuntos
Acetilgalactosamina/análogos & derivados , Hormônios/biossíntese , Sulfotransferases/metabolismo , Animais , Glicoproteínas/biossíntese , Humanos , Oligossacarídeos/biossíntese , Sulfotransferases/genética , Sulfotransferases/fisiologia , Distribuição TecidualRESUMO
We have identified and characterized an N-acetylgalactosamine-4-O-sulfotransferase designated dermatan-4-sulfotransferase-1 (D4ST-1) (GenBank(TM) accession number AF401222) based on its homology to HNK-1 sulfotransferase. The cDNA predicts an open reading frame encoding a type II membrane protein of 376 amino acids with a 43-amino acid cytoplasmic domain and a 316-amino acid luminal domain containing two potential N-linked glycosylation sites. D4ST-1 has significant amino acid identity with HNK-1 sulfotransferase (21.4%), N-acetylgalactosamine-4-O-sulfotransferase 1 (GalNAc-4-ST1) (24.7%), N-acetylgalactosamine-4-O-sulfotransferase 2 (GalNAc-4-ST2) (21.0%), chondroitin-4-O-sulfotransferase 1 (27.3%), and chondroitin-4-O-sulfotransferase 2 (22.8%). D4ST-1 transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. D4ST-1 shows a strong preference in vitro for sulfate transfer to IdoUAalpha1,3GalNAcbeta1,4 that is flanked by GlcUAbeta1,3GalNAcbeta1,4 as compared with IdoUAalpha1,3GalNAcbeta1,4 flanked by IdoUAalpha1,3GalNAcbeta1,4. The specificity of D4ST-1 when assayed in vitro suggests that the addition of sulfate to GalNAc occurs immediately after epimerization of GlcUA to IdoUA. The open reading frame of D4ST-1 is encoded by a single exon located on human chromosome 15q14. Northern blot analysis reveals a single 2.4-kilobase transcript. D4ST-1 message is expressed in virtually all tissues at some level but is most highly expressed in pituitary, placenta, uterus, and thyroid. The properties of D4ST-1 indicate that sulfation of the GalNAc moieties in dermatan is mediated by a distinct GalNAc-4-O-sulfotransferase and occurs following epimerization of GlcUA to IdoUA.
Assuntos
Dermatan Sulfato/química , Sulfotransferases/química , Sulfotransferases/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Northern Blotting , Células CHO , Sequência de Carboidratos , Cromatografia em Gel , Cromossomos Humanos Par 15 , Clonagem Molecular , Cricetinae , DNA Complementar/metabolismo , Relação Dose-Resposta a Droga , Éxons , Humanos , Modelos Químicos , Modelos Genéticos , Dados de Sequência Molecular , Análise de Sequência com Séries de Oligonucleotídeos , Fases de Leitura Aberta , Ligação Proteica , Estrutura Terciária de Proteína , RNA Mensageiro/metabolismo , Homologia de Sequência de Aminoácidos , Sulfotransferases/biossíntese , Fatores de Tempo , Distribuição Tecidual , TransfecçãoRESUMO
Recognition of carbohydrates on glycosylated molecules typically requires multivalent interactions with receptors. Monovalent forms of terminal saccharides engaged by the receptor binding sites typically display weak affinities in the mm range and poor specificity. In contrast, multivalent forms of the same saccharides are bound with strong affinity (10(-7)-10(-9) m) and significantly greater specificity. Although multivalency can readily account for increased affinity, the molecular basis for enhanced specificity is not well understood. We have examined the specificity of the cysteine-rich domain of the mannose/GalNAc-4-SO4 receptor using monovalent and multivalent forms of the trisaccharide GalNAcbeta1,4GlcNAcbeta1,2Manalpha (GGnM) sulfated at either the C4 (S4GGnM) or C3 (S3GGnM) hydroxyl of the terminal GalNAc. Monovalent S4GGnM and S3GGnM have K(i) values of 25.8 and 16.2 microm, respectively. Multivalent conjugates of the same GalNAc-4-SO4- and GalNAc-3-SO4-bearing trisaccharides (6.7 mol of trisaccharide/mol of bovine serum albumin) have K(i) values of 0.013 and 0.170 microm, respectively. The 2000-fold versus 95-fold change in affinity seen for the multivalent forms of these 4-sulfated and 3-sulfated trisaccharides reflects a difference in the impact of conformational entropy. A large fraction of the SO4-3-GalNAc structures exists in a form that is not favorable for binding to the Cys-rich domain. This reduces the effective concentration of SO4-3-GalNAc as compared with SO4-4-GalNAc under the same conditions and results in a markedly lower association rate. This difference in association rate accounts for the 12-fold difference in the rate of clearance from the blood seen with S4GGnM-BSA and S3GGnM-BSA in vivo.
Assuntos
Acetilgalactosamina/metabolismo , Lectinas de Ligação a Manose , Manose/metabolismo , Receptores de Superfície Celular/metabolismo , Trissacarídeos/metabolismo , Acetilgalactosamina/análogos & derivados , Animais , Sítios de Ligação , Ligação Competitiva , Sequência de Carboidratos , Bovinos , Membrana Celular/metabolismo , Cisteína , Entropia , Cinética , Antígenos CD15/análogos & derivados , Ligantes , Dados de Sequência Molecular , Oligossacarídeos/metabolismo , Receptores de Superfície Celular/química , Soroalbumina Bovina/metabolismo , Especificidade por Substrato , Ressonância de Plasmônio de Superfície , Trissacarídeos/químicaAssuntos
Acetilgalactosamina/metabolismo , Lectinas de Ligação a Manose , Receptores Imunológicos/fisiologia , Acetilgalactosamina/análogos & derivados , Animais , Metabolismo dos Carboidratos , Subunidade alfa de Hormônios Glicoproteicos/metabolismo , Humanos , Hormônio Luteinizante/metabolismo , Oligossacarídeos/metabolismo , Receptores Imunológicos/metabolismo , Vertebrados/metabolismoRESUMO
We have identified and characterized an N-acetylgalactosamine-4-O-sulfotransferase designated GalNAc-4-ST2 (GenBank(TM) accession number ) based on its homology to HNK-1 sulfotransferase (HNK-1 ST). The cDNA predicts an open reading frame encoding a type II membrane protein of 443 amino acids with a 12-amino acid cytoplasmic domain, a 23-amino acid transmembrane domain, and a 408-amino acid luminal domain containing four potential N-linked glycosylation sites. GalNAc-4-ST2 displays a high degree of amino acid sequence identity with GalNAc-4-ST1 (46%), HNK-1 ST (23%), chondroitin 4-O-sulfotransferase-1 (C4ST-1) (27%), and chondroitin 4-O-sulfotransferase-2 (C4ST-2) (24%). GalNAc-4-ST2 transfers sulfate to the C-4 hydroxyl of terminal beta1,4-linked GalNAc in the sequence GalNAc-beta1,4GlcNAcbeta-R found on N-linked oligosaccharides and nonterminal beta1,4-linked GalNAc in chondroitin and dermatan. The translated region of GalNAc-4-ST2 is encoded by five exons located on human chromosome 18q11.2. Northern blot analysis reveals a 2.1-kilobase transcript. GalNAc-4-ST2 message is most highly expressed in trachea and to a lesser extent in heart, liver, pancreas, salivary gland, and testis. The I.M.A.G.E. cDNA clone 49547 contains a putative GalNAc-4-ST2 splice form with an open reading frame encoding a protein of 358 amino acids that lacks the transmembrane domain and the stem region. This form of GalNAc-4-ST2 is not retained by transfected cells and is active against chondroitin but not terminal beta1,4-linked GalNAc. Thus, as with GalNAc-4-ST1, sequences N-terminal to the catalytic domain contribute to the specificity of GalNAc-4-ST2 toward terminal beta1,4-linked GalNAc.
Assuntos
Sulfotransferases/genética , Acetilgalactosamina/metabolismo , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , DNA Complementar/genética , Humanos , Dados de Sequência Molecular , Alinhamento de Sequência , Análise de Sequência , Sulfatos/metabolismo , Sulfotransferases/metabolismo , TransfecçãoRESUMO
N-Linked oligosaccharides terminating with the sequence SO(4)-4-GalNAcbeta1,4GlcNAcbeta1,2Manalpha are present on the pituitary hormones lutropin (LH), thyrotropin, and pro-opiomelanocortin. The sulfated structures on LH are essential for expression of its biologic function in vivo. We have cloned the N-acetylgalactosamine-4-sulfotransferase (GalNAc-4-ST1, GenBank(TM) accession number ), which mediates sulfate addition to the N-linked oligosaccharides on LH and other pituitary glycoproteins with terminal (beta1,4-linked GalNAc based on its homology to HNK-1 sulfotransferase (HNK-1 ST). GalNAc-4-ST1 displays 23% identity to HNK-1 ST and 28% to chondroitin 4-sulfotransferase 1 (C4ST-1) and 26% to chondroitin 4-sulfotransferase 2 (C4ST-2). The cDNA predicts a type II transmembrane protein of 424 amino acids with four potential N-linked glycosylation sites and a single membrane-spanning domain. GalNAc-4-ST1 has putative 5'-phosphosulfonate and 3'-phosphate binding sites. Three more carboxyl-terminal regions of unknown function also show a high degree of identity with HNK-1 ST, C4ST-1, and C4ST-2. The membrane-bound form of GalNAc-4-ST1 transfers sulfate to GalNAcbeta1, 4GlcNAcbeta-R but not to chondroitin, whereas truncated forms of GalNAc-4-ST1 that are released into the medium transfer sulfate to both GalNAcbeta1,4GlcNAcbeta-R and chondroitin. The first 118 amino acids of GalNAc-4-ST1 appear to contribute to both its activity and specificity for terminal beta1,4-linked GalNAc. GalNAc-4-ST1 also efficiently transfers sulfate to N-linked oligosaccharides on native LH and other glycoproteins terminating with beta1,4-linked GalNAc. A single transcript of 2.4 kilobases is most highly expressed in the pituitary and other regions of the central nervous system. The GalNAc-4-ST1 gene is located on human chromosome 19q13.1.
Assuntos
Hormônios Adeno-Hipofisários/metabolismo , Sulfotransferases/genética , Sulfotransferases/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Células CHO , Sequência de Carboidratos , Mapeamento Cromossômico , Cromossomos Humanos Par 19 , Clonagem Molecular , Cricetinae , Humanos , Hormônio Luteinizante/metabolismo , Dados de Sequência Molecular , Oligossacarídeos/química , Hormônios Adeno-Hipofisários/química , Pró-Opiomelanocortina/metabolismo , Ratos , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Tireotropina/metabolismo , TransfecçãoRESUMO
The circulatory half-life of the glycoprotein hormone lutropin (LH) is precisely regulated by the mannose (Man)/GalNAc-4-SO(4) receptor expressed in hepatic endothelial cells. Rapid clearance from the circulation contributes to the episodic rise and fall of LH levels that is essential for maximal stimulation of the G protein-coupled LH receptor. We have defined two molecular forms of the Man/GalNAc-4-SO(4) receptor that differ in ligand specificity, cell and tissue expression, and function. The form expressed by hepatic endothelial cells binds GalNAc-4-SO(4)-bearing ligands and regulates hormone circulatory half-life, whereas the form expressed by macrophages binds Man-bearing ligands and may play a role in innate immunity. We demonstrate that the GalNAc-4-SO(4)-specific form in hepatic endothelial cells is dimeric whereas the Man-specific form in lung macrophages is monomeric, accounting for the different ligand specificities of the receptor expressed in these tissues. Two cysteine-rich domains, each of which binds a single GalNAc-4-SO(4), are required to form stable complexes with LH. The kinetics of LH binding by the GalNAc-4-SO(4)-specific form of the receptor in conjunction with its rate of internalization from the cell surface make it likely that only two of the four terminal GalNAc-4-SO(4) moieties present on native LH are engaged before receptor internalization. As a result, the rate of hormone clearance will remain constant over a wide range of LH concentrations and will not be sensitive to variations in the number of terminal GalNAc-4-SO(4) moieties as long as two or more are present on multiple oligosaccharides.
Assuntos
Fígado/metabolismo , Pulmão/metabolismo , Hormônio Luteinizante/química , Hormônio Luteinizante/metabolismo , Lectinas de Ligação a Manose , Receptores de Superfície Celular/química , Receptores de Superfície Celular/metabolismo , Animais , Células CHO , Cricetinae , Dimerização , Endotélio/metabolismo , Cinética , Modelos Moleculares , Conformação Proteica , Ratos , Receptores de Superfície Celular/isolamento & purificação , Receptores do LH/metabolismo , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/metabolismo , Ressonância de Plasmônio de Superfície , TransfecçãoRESUMO
The large array of different glycolipids described in mammalian tissues is a reflection, in part, of diverse glycosyltransferase expression. Herein, we describe the cloning of a UDP-galactose: beta-d-galactosyl-1,4-glucosylceramide alpha-1, 3-galactosyltransferase (iGb(3) synthase) from a rat placental cDNA expression library. iGb(3) synthase acts on lactosylceramide, LacCer (Galbeta1,4Glcbeta1Cer) to form iGb(3) (Galalpha1,3Galbeta1, 4Glcbeta1Cer) initiating the synthesis of the isoglobo-series of glycosphingolipids. The isolated cDNA encoded a predicted protein of 339 amino acids, which shows extensive homology (40-50% identity) to members of the ABO gene family that includes: murine alpha1, 3-galactosyltransferase, Forssman (Gb(5)) synthase, and the ABO glycosyltransferases. In contrast to the murine alpha1, 3-galactosyltransferase, iGb(3) synthase preferentially modifies glycolipids over glycoprotein substrates. Reverse transcriptase-polymerase chain reaction revealed a widespread tissue distribution of iGb(3) synthase RNA expression, with high levels observed in spleen, thymus, and skeletal muscle. As an indirect consequence of the expression cloning strategy used, we have been able to identify several potential glycolipid biosynthetic pathways where iGb(3) functions, including the globo- and isoglobo-series of glycolipids.
Assuntos
Galactosiltransferases/genética , Galactosiltransferases/fisiologia , Globosídeos/metabolismo , Glicoesfingolipídeos/biossíntese , Sistema ABO de Grupos Sanguíneos/metabolismo , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais/química , Sequência de Bases , Células CHO , Separação Celular , Cromatografia em Camada Fina , Clonagem Molecular , Cricetinae , DNA Complementar/metabolismo , Feminino , Citometria de Fluxo , Biblioteca Gênica , Glicosídeo Hidrolases/metabolismo , Modelos Biológicos , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Placenta/metabolismo , Plasmídeos/metabolismo , Ratos , Ratos Long-Evans , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Especificidade por Substrato , Distribuição Tecidual , TransfecçãoRESUMO
We have cloned Gb(3) synthase, the key alpha1, 4-galactosyltransferase in globo-series glycosphingolipid (GSL) synthesis, via a phenotypic screen, which previously yielded iGb(3) synthase, the alpha1,3-galactosyltransferase required in isoglobo-series GSL (Keusch, J. J., Manzella, S. M., Nyame, K. A., Cummings, R. D., and Baenziger, J. U. (2000) J. Biol. Chem. 33). Both transferases act on lactosylceramide, Galbeta1,4Glcbeta1Cer (LacCer), to produce Gb(3) (Galalpha1,4LacCer) or iGb(3) (Galalpha1, 3LacCer), respectively. GalNAc can be added sequentially to either Gb(3) or iGb(3) yielding globoside and Forssman from Gb(3), and isogloboside and isoForssman from iGb(3). Gb(3) synthase is not homologous to iGb(3) synthase but shows 43% identity to a human alpha1,4GlcNAc transferase that transfers a UDP-sugar in an alpha1, 4-linkage to a beta-linked Gal found in mucin. Extensive homology (35% identity) is also present between Gb(3) synthase and genes in Drosophila melanogaster and Arabidopsis thaliana, supporting conserved expression of an alpha1,4-glycosyltransferase, possibly Gb(3) synthase, throughout evolution. The isolated Gb(3) synthase cDNA encodes a type II transmembrane glycosyltransferase of 360 amino acids. The highest tissue expression of Gb(3) synthase RNA is found in the kidney, mesenteric lymph node, spleen, and brain. Gb(3) glycolipid, also called P(k) antigen or CD77, is a known receptor for verotoxins. CHO cells that do not express Gb(3) and are resistant to verotoxin become susceptible to the toxin following transfection with Gb(3) synthase cDNA.
Assuntos
Galactosiltransferases/genética , Sequência de Aminoácidos , Animais , Arabidopsis , Toxinas Bacterianas/farmacologia , Sequência de Bases , Células CHO , Separação Celular , Cromatografia em Camada Fina , Clonagem Molecular , Sequência Conservada , Cricetinae , DNA Complementar/metabolismo , Bases de Dados Factuais , Drosophila , Citometria de Fluxo , Galactosiltransferases/metabolismo , Biblioteca Gênica , Globosídeos/metabolismo , Glicolipídeos/metabolismo , Glicoesfingolipídeos/biossíntese , Humanos , Dados de Sequência Molecular , Família Multigênica , Mutagênese Sítio-Dirigida , Placenta/metabolismo , Ratos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Homologia de Sequência de Aminoácidos , Toxina Shiga I , Toxinas Shiga , Distribuição Tecidual , TransfecçãoRESUMO
A critical element of lutropin bioactivity in vivo is its rapid removal from the blood by a receptor, located in hepatic endothelial cells, that recognizes the terminal sulfated carbohydrate structure SO4-4-GalNAcbeta1,4GlcNAcbeta1,2Manalpha (S4GGnM). We have previously shown that the macrophage mannose (Man)-receptor cDNA directs the synthesis of a protein that binds oligosaccharides with either terminal S4GGnM or terminal Man, at independent sites. We now show that the cysteine-rich (Cys-Rich) domain at the N terminus of the Man/S4GGnM receptor accounts for binding of oligosaccharides with terminal GalNAc-4-SO4, whereas calcium-dependent carbohydrate recognition domains (CRDs) account for binding of ligands containing terminal Man. The Cys-Rich domain is thus a previously unrecognized carbohydrate binding motif. Cys-Rich domains have been described on the three other members of the endocytic C-type lectin family of receptors. The structural relationship of these receptors to the Man/S4GGnM receptor raises the possibility that their Cys-Rich domains also bind carbohydrate moieties and contribute to their function.
Assuntos
Lectinas Tipo C , Hormônio Luteinizante/metabolismo , Lectinas de Ligação a Manose , Oligossacarídeos/química , Receptores de Superfície Celular/química , Receptores de Superfície Celular/metabolismo , Animais , Sítios de Ligação , Células CHO , Configuração de Carboidratos , Sequência de Carboidratos , Cricetinae , Cinética , Hormônio Luteinizante/química , Receptor de Manose , Modelos Moleculares , Dados de Sequência Molecular , Oligossacarídeos/metabolismo , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/metabolismo , TransfecçãoRESUMO
Lutropin (LH) and other glycoproteins bearing oligosaccharides with the terminal sequence SO4-4-GalNAcbeta1,4GlcNAcbeta1,4Man- (S4GGnM) are rapidly removed from the circulation by an S4GGnM-specific receptor (S4GGnM-R) expressed at the surface of hepatic endothelial cells. The S4GGnM-R isolated from rat liver is closely related to the macrophage mannose-specific receptor (Man-R) isolated from rat lung both antigenically and structurally. The S4GGnM-R and Man-R isolated from these tissues nonetheless differ in their ability to bind ligands bearing terminal GalNAc-4-SO4 or Man. In this paper, we have explored the structural relationship between the Man-R and the S4GGnM-R by examining the properties of the recombinant Man-R in the form of a transmembrane protein and a soluble chimeric fusion protein in which the transmembrane and cytosolic domains have been replaced by the Fc region of human IgG1. Like the S4GGnM-R isolated from liver, the chimeric fusion protein is able to bind ligands terminating with GalNAc-4-SO4 and Man at independent sites. When expressed in CHO cells the recombinant Man-R is able to mediate the uptake of ligands bearing either terminal GalNAc-4-SO4 or terminal Man. We propose that the Man-R be renamed the Man/S4GGnM receptor on the basis of its multiple and independent specificities.
Assuntos
Dissacarídeos , Endotélio Vascular/metabolismo , Lectinas Tipo C , Macrófagos/metabolismo , Lectinas de Ligação a Manose , Manose , Oligossacarídeos/metabolismo , Receptores de Superfície Celular/biossíntese , Substituição de Aminoácidos , Animais , Células CHO , Metabolismo dos Carboidratos , Sequência de Carboidratos , Cromatografia de Afinidade , Cricetinae , DNA Complementar , Humanos , Cinética , Fígado/metabolismo , Hormônio Luteinizante , Receptor de Manose , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Oligossacarídeos/química , Ratos , Receptores de Superfície Celular/isolamento & purificação , Receptores de Superfície Celular/metabolismo , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/isolamento & purificação , Proteínas Recombinantes de Fusão/metabolismo , Especificidade por Substrato , TransfecçãoRESUMO
Glycoproteins, such as the glycoprotein hormone lutropin (LH), bear oligosaccharides terminating with the sequence SO4-4GalNAcbeta1, 4GlcNAcbeta1,2Manalpha (S4GGnM) and are rapidly removed from the circulation by a receptor present in hepatic endothelial cells and Kupffer cells. Rapid removal from the circulation is essential for attaining maximal hormone activity in vivo. We have isolated a protein from rat liver which has the properties expected for the S4GGnM-specific receptor (S4GGnM-R). The S4GGnM-R is closely related to the macrophage mannose receptor (Man-R) both antigenically and structurally. At least 12 peptides prepared from the S4GGnM-R have amino acid sequences that are identical to those of the Man-R. Nonetheless, the ligand binding properties of the S4GGnM-R and the Man-R differ in a number of respects. The S4GGnM-R binds to immobilized LH but not to immobilized mannose, whereas the Man-R binds to immobilized mannose but not to immobilized LH. When analyzed using a binding assay that precipitates receptor ligand complexes with polyethylene glycol, the S4GGnM-R is able to bind S4GGnM-bovine serum albumin (S4GGnM-BSA) conjugates whereas the Man-R is not. In contrast both the S4GGnM-R and the Man-R are able to bind Man-BSA. Monosaccharides that inhibit binding of Man-BSA by the Man-R enhance binding by the S4GGnM-R. Oligosaccharides terminating with S4GGnM and those terminating with Man are bound at independent sites on the S4GGnM-R. The S4GGnM-R present in hepatic endothelial cells may account for clearance of glycoproteins bearing oligosaccharides terminating with S4GGnM and glycoproteins bearing oligosaccharides terminating with either mannose, fucose, or N-acetylglucosamine.
Assuntos
Fígado/metabolismo , Receptores de Superfície Celular/isolamento & purificação , Receptores de Superfície Celular/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Carboidratos , Cromatografia de Afinidade , Glicoproteínas , Indicadores e Reagentes , Cinética , Dados de Sequência Molecular , Peso Molecular , Oligossacarídeos/metabolismo , Fragmentos de Peptídeos/química , Mapeamento de Peptídeos , Radioimunoensaio , Ratos , Ratos Sprague-Dawley , Receptores de Superfície Celular/químicaRESUMO
Successful pregnancy is dependent upon an array of signaling proteins secreted by the trophoblast cells of the placenta. Among these is a group of proteins related to pituitary prolactin, known as the prolactin/growth hormone family. These proteins are expressed at specific times during gestation and synthesized in distinct trophoblast cell types in the rat placenta. We report here that select members of this family, prolactin-like protein (PLP-A), PLP-B, PLP-C, decidual/trophoblast PRP, and placental lactogen I variant, only which are expressed in the spongiotrophoblast, late in rat placental development bear Asn-linked oligosaccharides terminating with NeuAcalpha2,6GalNAcbeta1,4GlcNAcbeta-R. This reflects the concurrent expression of these prolactin/growth hormone family members with the peptide-specific beta1,4GalNAc-transferase and an alpha2,6-sialyltransferase, which can add sialic acid to terminal beta1,4-linked GalNAc. We have determined that at least one of the prolactin-like proteins, PLP-A, is recognized by the protein-specific GalNAc-transferase. The presence of NeuAcalpha2, 6GalNAcbeta1,4GlcNAcbeta-R on only a limited number of glycoproteins synthesized by the spongiotrophoblasts between mid gestation and birth reflects the need for both the GalNAc-transferase and the peptide recognition determinant for efficient addition of GalNAc. Thus, expression of the GalNAc-transferase and specific members of the prolactin/growth hormone family is developmentally regulated in the rat placenta, suggesting a physiological role for the terminal NeuAcalpha2,6GalNAcbeta1,4GlcNAcbeta-R sequence on Asn-linked oligosaccharides of these proteins.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Oligossacarídeos/metabolismo , Placenta/metabolismo , Proteínas da Gravidez/metabolismo , Animais , Feminino , Oligossacarídeos/genética , Gravidez , Proteínas da Gravidez/genética , Ratos , Ratos Sprague-DawleyRESUMO
A phenotypic cloning approach was used to isolate a canine cDNA encoding Forssman glycolipid synthetase (FS; UDP-GalNAc:globoside alpha-1,3-N-acetylgalactosaminyltransferase; EC 2.4.1.88). The deduced amino acid sequence of FS demonstrates extensive identity to three previously cloned glycosyltransferases, including the enzymes responsible for synthesis of histo-blood group A and B antigens. These three enzymes, like FS, catalyze the addition of either N-acetylgalactosamine (GalNAc) or galactose (Gal) in alpha-1,3-linkage to their respective substrates. Despite the high degree of sequence similarity among the transferases, we demonstrate that the FS cDNA encodes an enzyme capable of synthesizing Forssman glycolipid, and demonstrates no GalNAc or Gal transferase activity when closely related substrates are examined. Thus, the FS cDNA is a novel member of the histo-blood group ABO gene family that encodes glycosyltransferases with related but distinct substrate specificity. Cloning of the FS cDNA will allow a detailed dissection of the roles Forssman glycolipid plays in cellular differentiation, development, and malignant transformation.
Assuntos
Antígeno de Forssman/genética , N-Acetilgalactosaminiltransferases/genética , Sistema ABO de Grupos Sanguíneos , Sequência de Aminoácidos , Animais , Sequência de Bases , Células COS , Sequência de Carboidratos , Clonagem Molecular , DNA Complementar/genética , Cães , Dados de Sequência Molecular , Proteínas Recombinantes , Alinhamento de Sequência , Homologia de Sequência de AminoácidosRESUMO
Carbohydrates attached to proteins and lipids characteristically display complex and heterogeneous structures. However, it is becoming increasingly clear that carbohydrates with definite biological functions also exhibit unique structural features. A number of glycoproteins and glycolipids have been shown to bear oligosaccharides containing sulfate. Often, addition of a sulfate moiety turns a relatively common structural motif into a unique carbohydrate with the potential to be recognized by a specific receptor or lectin. This is clearly the case in three systems in which sulfated oligosaccharides have been shown to play a well-defined biological role: 1) control of the circulatory half-life of luteinizing hormone, 2) symbiotic interactions between leguminous plants and nitrogen-fixing bacteria, and 3) homing of lymphocytes to lymph nodes. The rapidly growing list of glycoproteins and glycolipids identified as bearing sulfated oligosaccharides suggests that sulfated carbohydrates play important biological roles in numerous other systems as well.-Hooper, L. V., Manzella, S. M., Baenziger, J. U. From legumes to leukocytes: biological roles for sulfated carbohydrates.
Assuntos
Carboidratos/fisiologia , Fabaceae , Leucócitos , Plantas Medicinais , Sulfatos/metabolismo , Animais , Configuração de Carboidratos , Sequência de Carboidratos , Carboidratos/química , Fabaceae/química , Humanos , Hormônio Luteinizante/química , Hormônio Luteinizante/fisiologia , Dados de Sequência Molecular , Oligossacarídeos/química , Oligossacarídeos/metabolismoAssuntos
Gonadotropina Coriônica/química , Hormônio Foliculoestimulante/química , Hormônio Luteinizante/química , Oligossacarídeos/química , Tireotropina/química , Sequência de Aminoácidos , Animais , Sequência de Carboidratos , Gonadotropina Coriônica/metabolismo , Hormônio Foliculoestimulante/metabolismo , Glicosilação , Humanos , Hormônio Luteinizante/metabolismo , Dados de Sequência Molecular , Relação Estrutura-Atividade , Tireotropina/metabolismoRESUMO
The circulatory half-life of the mammalian glycoprotein hormone lutropin is controlled by its unique Asn-linked oligosaccharides, which terminate with the sequence SO4-4-GalNAc beta 1,4GlcNAc. A cluster of basic amino acids essential for recognition of the alpha subunit by the glycoprotein hormone:N-acetylgalactosaminyltransferase is located within two turns of an alpha helix (Mengeling, B.J., Manzella, S.M., and Baenziger, J.U. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 502-506). The amino acids within this region are virtually invariant in the alpha subunits of all vertebrates, indicating that the recognition determinant utilized by the N-acetylgalactosaminyltransferase has been conserved in species ranging from teleost fish to mammals. We demonstrate that the glycoprotein hormone:N-acetylgalactosaminyltransferase and the N-acetylgalactosamine-4-sulfotransferase responsible for the synthesis of these unique sulfated oligosaccharides are expressed in the pituitaries of vertebrates ranging from teleost fish to mammals. Furthermore, we show that Asn-linked oligosaccharides terminating with SO4-4-GalNAc beta 1,4GlcNAc are present on the alpha and beta subunits of the salmon glycoprotein hormone GTH II. Asn-linked oligosaccharides terminating with SO4-4-GalNAc beta 1,4GlcNAc are unique structural features of the glycoprotein hormones that have been conserved during vertebrate evolution, suggesting they are critical for the expression of hormone biologic activity.
Assuntos
Evolução Biológica , Subunidade alfa de Hormônios Glicoproteicos/química , Subunidade alfa de Hormônios Glicoproteicos/metabolismo , Hormônio Luteinizante/química , Hormônio Luteinizante/metabolismo , N-Acetilgalactosaminiltransferases/metabolismo , Oligossacarídeos/química , Hipófise/metabolismo , Sequência de Aminoácidos , Animais , Configuração de Carboidratos , Sequência de Carboidratos , Galinhas , Gonadotropina Coriônica/metabolismo , Sequência Conservada , Meia-Vida , Humanos , Dados de Sequência Molecular , Oligossacarídeos/isolamento & purificação , Oncorhynchus kisutch , Estrutura Secundária de Proteína , Rana catesbeiana , Ratos , Especificidade por Substrato , Sulfatos/análise , Transferrina/metabolismo , Tartarugas , VertebradosRESUMO
The pituitary glycoprotein hormone lutropin is characterized by its pulsatile appearance in the bloodstream which is important for the expression of its biological activity in the ovary. We have previously shown that lutropin bears unique Asn-linked oligosaccharides terminating with GalNAc-4-SO4 which allow the hormone to be rapidly cleared from the bloodstream via a specific receptor in the liver, thus contributing to its pulsatile appearance in the circulation. Furthermore, we have found that carbonic anhydrase VI, synthesized by the submaxillary gland and secreted into the saliva, also bears Asn-linked oligosaccharides terminating with GalNAc-4-SO4, suggesting that this unique sulfated structure mediates other biological functions in addition to rapid clearance from the circulation. We report here the purification of a GalNAc-4-sulfotransferase which transfers sulfate to terminal beta 1,4-linked GalNAc on Asn-linked oligosaccharides. We show that the purified submaxillary gland enzyme has kinetic parameters identical to the pituitary enzyme, indicating that the same sulfotransferase is responsible for the sulfation of lutropin oligosaccharides in pituitary and carbonic anhydrase VI oligosaccharides in submaxillary gland. This GalNAc-4-sulfotransferase has an apparent molecular mass of 128 kDa and can be specifically photoaffinity radiolabeled with 3',5'-ADP, a competitive inhibitor of sulfotransferase activity. The acceptor specificity of this GalNAc-4-sulfotransferase indicates that it is able to transfer sulfate to terminal GalNAc beta 1,4GlcNAc on both N- and O-glycosidically linked oligosaccharides, suggesting that this enzyme is also responsible for the sulfation of O-linked glycans on proopiomelanocortin.