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Biocell ; 30(2): 295-300, 2006 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16972554

RESUMO

We assessed the ascorbic acid (AA) supplementation on the myenteric neurons in the duodenum of rats. Fifteen rats with 90 days of age were divided into three groups: control (C), diabetics (D) and ascorbic acid treated diabetics (DA). After 120 days of daily treatment with AA, the duodenum was submitted to the NADH-diaphorase (NADH-d) histochemical technique, which allowed us to evaluate the neuronal density in an area of 8.96 mm2 for each duodenum, and also to measure the cellular profile area of 500 neurons per group. The supplementation promoted an increase on AA levels. The neuronal density (p < 0.05) was higher in the group DA when compared to group D. There were no significant differences in the neuronal areas, when we compared groups C (204 +/- 16.5) and D (146.3 +/- 35.84) to groups D and DA (184.5 +/- 5.6) (p > 0.05). The AA-supplementation avoided the density reduction of the NADHd myenteric neurons in the duodenum of diabetic rats.


Assuntos
Ácido Ascórbico/farmacologia , Diabetes Mellitus Experimental , Di-Hidrolipoamida Desidrogenase/metabolismo , Duodeno/efeitos dos fármacos , Plexo Mientérico/efeitos dos fármacos , Plexo Mientérico/enzimologia , Neurônios/efeitos dos fármacos , Neurônios/enzimologia , Animais , Ácido Ascórbico/administração & dosagem , Peso Corporal/efeitos dos fármacos , Membrana Celular/efeitos dos fármacos , Suplementos Nutricionais , Duodeno/citologia , Duodeno/enzimologia , Masculino , Ratos , Ratos Wistar
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