Comparative analysis of human serum albumin adsorption and complement activation for intraocular lenses.

Intraocular liquid, in contrast to blood, has no cellular components; therefore, proteins (human serum albumin [HSA], and [alpha, beta, gamma] globulins) are the major components that determine patients' response to the intraocular lens (IOL) surface. In addition to the amount of adsorbed proteins, the possibility of its conformational changes, including conformational changes of globulins C1 and C3 that respond for the activation of the complements system by the classical and alternative pathways, cannot be excluded. The interaction between IOLs and protein components of intraocular liquid directly influences the ocular exudative reaction in the early postoperational period, the intensity of cellular and pigmental scurf on the surface of the IOLs, and the state of endothelial cells of the cornea in the distant postoperational period. Our goal was to compare the interaction of commercial IOLs made from polymethylmethacrylate, silicone, poly-2-hydroxyethyl methacrylate (p-HEMA), and copolymer p-HEMA with collagen with HSA and the complement system. The total internal reflection fluorescence (TIRF) method and hemolytic assay were used for this task, respectively. It has been demonstrated that the probability of biocompatibility of commercially produced IOLs on the stage of protein adsorption can be evaluated using the kinetic of HSA-fluorescein isothiocyanate adsorption onto the IOL surface by the TIRF METHOD: In the case of IOLs from p-HEMA, a negative correlation was shown between the degree of irreversible adsorption of HSA and the minimum relative rate constant of the surface-induced complement activation. We did not find any correlation between hydrophilicity/hydrophobicity of lenses and their adsorptional properties including complement activation. From suggested adsorptional criteria in vitro for biocompatible surfaces, the hydrogel lens from p-HEMA has a lower probability of biocompatibility in comparison with other IOLs.

[Use of the fluorescence method in the study of protein adsorption onto the surface of intraocular lenses]. / Primenenie metoda fliuorestsentsii dlia issledovaniia adsorbtsii belkov na poverkhnosti intraokuliarnykh linz.

The paper shows it possible to use the method of total internal reflection-fluorescence for study of the kinetics of protein adsorption onto the surface of intraocular lenses (IOL) made from optically transparent hydrophobic polymers and hydrogels. The nature of the materials (polymethylmethacrylate, silicone, p-HEMA, p-HEMA-collagen copolymer) used for IOL on the adsorption and desorption of human serum albumin was studied.

[The activation of the proliferation of human corneal endothelium in malignant tumors of various sites and diabetes mellitus]. / Aktivatsiia proliferatsii éndoteliia rogovitsy cheloveka pri zlokachestvennykh opukholiakh razlichnoi lokalizatsii i sakharnom diabete.

The morphological investigation of corneal endothelium was carried out in 205 human eyes of patients with malignant tumors of different localization including the eye, and also with diabetes mellitus. The investigation was performed in flat specimens by electron microscopy. In all types of pathology mitotic figures were found in endothelial population, most frequently in cases of diabetes of moderate and severe degree. Simultaneously the endothelial cell density increased significantly comprising in case of choroidal melanoma 2416 cell/mm2, in cancer of different localization 2916 cell/mm2; in the control group in 40-60-year-old patients 2160 cell/mm2; in diabetes mellitus of moderate and severe degree 2770 cell/mm2, in the control group in 60-80-year-old patients 1975 cell/mm. Simultaneously the endothelial cell area decreased significantly, the number of small unchanged cells of mitosis product increased and that of large cells diminished. Therefore, it growth factors in the host are more active, mitotic division of corneal endothelial cells will become more active as well.

[The reparative potentials of human corneal endothelium from the aspect of age]. / Reparativnye vozmozhnosti éndoteliia rogovitsy cheloveka v vozrastnom aspekte.

Morphometric and cytospectrophotometric studies of the endothelium of human cornea in age aspect, from 20 to 80 years, has revealed the presence of nuclei with double content of DNA (4 c) irrespective of age, as well as binuclear cells. Their number increases with age. At the age of 20-40 years, 4 tetraploid nuclei and 3 binuclear cells were found, and after 80 years of age - 45 tetraploid nuclei and 11 binuclear cells per a cornea. This speaks about the ability of the endothelium cell of the human cornea to incomplete polyploidizing cellular mitosis-variant of a normal proliferation of the cells and is characteristic for long-living and slightly proliferating tissues. The increase of the number of polyploid cells in the corneal endothelium with age speaks about activation of adaptation mechanisms in the course of age involution.

[Experimental secondary cataract induced by immune complexes]. / Eksperimental'naia vtorichnaia katarakta, indutsirovannaia immunnymi kompleksami.

The aim of this experimental study was to elucidate the contribution of the immune complexes to the formation of a secondary cataract. The immune complexes are formed after the lens extraction in case specific antibodies to the lens and its capsule and to the lens mass are present in the circulation. The immune complexes, fixed on the posterior capsule, induce antibody-dependent fibrosis, in other words, a secondary cataract.