miR-140-3p is a potential differential biomarker in benign phyllodes tumors and fibroadenoma of the breast.

BACKGROUND: Benign phyllodes tumor (BPT) and fibroadenoma (FA) have some difficulties in differential diagnosis. BPT is often misdiagnosed as FA during the first operation and is not diagnosed until postoperative recurrence and reoperation. The intent of this research was to find and validate microRNAs (miRNAs) with significant differential expression between BPT and FA as novel potential differential biomarkers. METHODS: Tissue specimens from three BPT patients and three FA patients were selected to detect the expression of miRNAs by miRNA-Seq technique. Primary cells were extracted and cultured from fresh BPT and FA tissues by tissue-block culture. The expression of differentially expressed miRNA (DEmiRNA) was further verified by quantitative real-time polymerase chain reaction (qRT-PCR) in twelve BPT and eleven FA patient specimens as well as primary cells. Data with a P value < 0.05 were considered statistically significant. RESULTS: The miRNA-Seq results showed totally six DEmiRNA were identified, consisting of two downregulated genes and four upregulated genes in BPT. Further validation by qRT-PCR manifest that miR-140-3p was downregulated by approximately 70% in BPT. CONCLUSION: miR-140-3p could become potential differential biomarker for BPT and FA.

Visualization Experimental Study on Well Spacing Optimization of SAGD with a Combination of Vertical and Horizontal Wells.

For oil sand reservoirs, the steam-assisted gravity drainage (SAGD) technique is one of the most efficient thermal recovery technologies. However, the high oil viscosity and the severe heat loss seriously affect the production effect of SAGD in developing thin oil sand reservoirs by the traditional SAGD technology. Therefore, it is essential to improve the SAGD technology to enhance the recovery of the thin oil sand reservoir. In this paper, SAGD with a combination of vertical and horizontal well (VH-SAGD) technology was adopted, and the influence of different well spacings (horizontal distance between vertical steam injection wells and horizontal production wells) on the development of steam chambers was investigated. By the similarity criterion, the experimental parameters were obtained. After that, an improved 2D visualization physical model was designed with 9 × 9 high-precision thermocouples installed inside the device to monitor the real-time temperature. This experimental physical model can not only accurately capture the temperature distribution but also display the continuous change process of the chamber inside the model by the visible window. Finally, different well spacing cases (10, 15, and 20 cm) were tested to observe the development of the steam chamber and analyze the production data. Both the temperature distribution and visual window showed that the steam chamber has four obvious stages, and reasonable well spacing can promote the development of the steam chamber. When the well spacing is relatively small, the unswept area of the cold oil on both sides is large, and the area of the steam chamber overlaps more. When the well spacing is relatively moderate, the steam chamber is the most complete and the recovery factor is the highest. When the well spacing is relatively large, although the unswept area of the cold oil on both sides is small, the middle cold oil area is larger than the previous two groups. Therefore, the best well spacing for oil sand reservoirs of about 15 m thickness is 15-20 m, where the VH-SAGD method has a better displacement effect and higher oil recovery. The experimental conclusions can provide theoretical support for the production of thin oil sand reservoirs.