RESUMO
The white clover has been transformed with the Betaine Aldehyde Dehydrogenase (BADH) gene cloned from Atriplex hortensis by Agrobacterium tumefaciens. The relative electronic conductivity of the transgenic plants under 1% NaCl stress for 48 hours was about 20%, less than the control plant's relative electronic conductivity (more than 40%), these showed the cell membrane of the transgenic plants has been less injured than control plants under salt stress. The other experience showed that the transgenic plant could grow well in water culture included 0.5% NaCl for more than two weeks, but the control plants could not.
Assuntos
Agrobacterium tumefaciens/genética , Aldeído Oxirredutases/genética , Cloreto de Sódio/farmacologia , Transformação Genética , Trifolium/genética , Betaína-Aldeído Desidrogenase , Northern Blotting , Southern Blotting , Plantas Geneticamente Modificadas , Reação em Cadeia da Polimerase , Trifolium/efeitos dos fármacosRESUMO
In this paper we review recent advances in studies of several aspects of genome specific repetitive DNA sequences in wheat and related species. The available results demonstrate that genome specific repetitive DNA sequences are important components of genome specificity in wheat and related species. Research on genome specific repetitive DNA sequences is essential to the elucidation of genome function. The application of genome specific repetitive DNA sequences will aid molecular cytogenetic studies in wheat and related species and contributes to genetic improvement of common wheat.
