RESUMO
Phytochemical investigation of Senecio obtusatus Wall. ex DC led to the isolation of a novel alkaloid, senobtusin (1). Compound 1 possesses an atypical amidine moiety, and the structure was determined on the basis of extensive NMR analysis and HR-ESI-MS technique. This is the first report of this class of natural products obtained from family Asteraceae, and the systematic importance was also discussed.
Assuntos
Alcaloides/química , Senécio/química , Alcaloides/isolamento & purificação , Amidinas/química , China , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Estrutura Molecular , Compostos Fitoquímicos/química , Compostos Fitoquímicos/isolamento & purificaçãoRESUMO
OBJECTIVE: To study the effect of silverionized drinking water on erythrocyte membrane fluidity, serum lipids and vascular endothelial cells in tail-suspended rats. METHOD: Thirty male SD rats were randomly divided into ground control group (GC), simulated weightlessness control group (SC), simulated weightlessness and silverionized water drinking group (SS). Number of circulating endothelial cells (CEC), serum lipids and erythrocyte membranes fluidity was measured on the 21st day of tail suspension. RESULT: Levels of serum TC, TG, HDL-C, HDL-C/TC and erythrocyte membrane fluidity in SC rats were significantly lower than those in GC rats; LDL-C/HDL-C ratio and number of CEC in SC rats were markedly higher than those in GC rats. Levels of serum TC, LDL-C and LDL-C/HDL-C in SS rats were higher than those in SC rats; HDL-C/TC ratio and erythrocyte membrane fluidity in SS rats were lower than those in SC rats. CONCLUSION: Drinking silverionized water has a negative effect on lipid metabolism in tail-suspended rats.
Assuntos
HDL-Colesterol/efeitos dos fármacos , LDL-Colesterol/efeitos dos fármacos , Prata/farmacologia , Água/farmacologia , Simulação de Ausência de Peso , Animais , HDL-Colesterol/metabolismo , LDL-Colesterol/metabolismo , Células Endoteliais/efeitos dos fármacos , Elevação dos Membros Posteriores , Masculino , Fluidez de Membrana , Ratos , Ratos Sprague-Dawley , Triglicerídeos/metabolismoRESUMO
OBJECTIVE: To study the changes of erythrocyte membrane fluidity, serum lipid and vascular endothelial cell caused by simulated weightlessness in rats and the beneficial effect of spirulina. METHOD: Thirty male SD rats were divided into 3 groups: free control group (group A) and two simulated weightlessness groups (groups B, C). Rats in group A and B were fed with normal forage, and the rats in group C were fed with normal forage supplemented with 5% (W/W) spirulina. Water was taken ad libitum. RESULT: Levels of serum CHO, HDL, TG, HDL-C/CHO and erythrocyte membrane fluidity decreased significantly, and number of vascular endothelial cells in plasma increased markedly in group B as compared with those in group A; The ratio of LDL-C/HDL-C, and atherosclerosis index (AI) decreased, number of vascular endothelial cells significantly lowered; level of CHO, HDL-C and value of the IDmax of plasma as well as erythrocyte membrane fluidity remarkedly increased in group C compared with those in group B. CONCLUSION: Spirulina can improve the physiological conditions of erythrocyte membrane fluidity, serum lipid and vascular endothelial cell caused by simulated weightlessness in rats.
Assuntos
Proteínas de Bactérias/farmacologia , Cianobactérias , Endotélio Vascular/efeitos dos fármacos , Membrana Eritrocítica/efeitos dos fármacos , Lipídeos/sangue , Simulação de Ausência de Peso , Animais , Proteínas de Bactérias/administração & dosagem , Colesterol/sangue , Colesterol/metabolismo , Suplementos Nutricionais , Endotélio Vascular/citologia , Deformação Eritrocítica/efeitos dos fármacos , Deformação Eritrocítica/fisiologia , Membrana Eritrocítica/fisiologia , Metabolismo dos Lipídeos , Lipoproteínas/sangue , Lipoproteínas/efeitos dos fármacos , Lipoproteínas/metabolismo , Masculino , Fluidez de Membrana/efeitos dos fármacos , Fluidez de Membrana/fisiologia , Ratos , Ratos Sprague-Dawley , Spirulina , Triglicerídeos/sangue , Triglicerídeos/metabolismoRESUMO
Objective. To study the effect of dietary n-3 polyunsaturated fatty acid (PUFA) on the lipid metabolism of tail-suspended rats. Method. 49 male Sprague-Dawley rats were divided into five groups: tail-suspension group I, fed with 10 wt % corn oil, tail-suspension groups II, III, IV were fed with three levels of fish oil (0.5, 1.5 or 3.0 wt %; low, middle and high level of fish oil, respectively). Group V fed with 10 wt % corn oil served as normal control. Result. Viscoelasticity of cell membrane in group III was significantly higher than that of group I (P<0.01); Vascular endothelial cells (VEC) was less in group II compared to group I; Serum total cholesterol (TC) and triglyceride (TG) , serum lipoprotein were lower in groups II, III, IV compared to group I, the higher fish oil content in diet, the lower serum lipid concentration in microgravity. Conclusion. Dietary n-3 PUFA do have distinguished effect on lipid metabolism and n-3 PUFA have certain effects on prevention of atherosclerotic disease and coronary heart disease in rats.
