Expression of HIF-1α and Nestin in oral squamous cell carcinoma and its association with vasculogenic mimicry.

AIM: To evaluate and correlate the expression of HIF1-α and Nestin in tumor center and periphery of nonmetastatic, and recurrent oral squamous cell carcinoma (OSCC) and its association with vasculogenic mimicry. MATERIALS AND METHODS: About 60 histopathological proven cases of OSCC with proper tumor center and periphery were collected. Among them 25 are nonmetastatic, 25 metastatic, and 10 recurrent cases of OSCC. Immunohistochemical analysis of HIF, Nestin, and CD31/PAS (periodic acid Schiff) was done. RESULTS: Based on the extent of tumor cells stained, staining intensity and index score, expression of both HIF and Nestin was highly significant in periphery of metastatic OSCC with a P value of 0.003* and 0.001*. The total number of vessels expressed in nonmetastatic, metastatic, and recurrent OSCC was not significant but the overall expression of CD31/PAS was significant in the periphery of the tumor with a P value of 0.024*. Correlating the overall expression, HIF showed a positive relation with Nestin and CD31/PAS with a P value of 0.026* and 0.038* in nonmetastatic OSCC using Pearson's correlation coefficient analysis. CONCLUSION: Based on the above results hypoxia plays a vital role in cancer stem cells maintenance with the formation of vessel-like structures by tumor cells at an early stage of cancer development.

Age Estimation using Cephalometrics-A Cross-Sectional Study among Teenagers of Salem District, Tamil Nadu.

Background: Age estimation is important not only in identifying dead body of a person but also in living persons since there is an increasing rate of juvenile delinquencies recorded every year. To avoid foul play by age fabrication, legal age estimation becomes important. Facial growth alteration takes place in the jawbones as age advances which can be observed with lateral cephalometry. Thus, the aim of the study is to create a regression formula for age estimation using cephalometrics of teenagers in Salem population. Materials and Methods: A cross-sectional study was done using 770 lateral cephalometrics of teenagers (13-19 yrs) in Salem population. Nine cephalometric points with two linear hard tissue measurements (condylion to mandibular plane (AFH) and palatal plane to menton (PFH)) and one angular soft tissue measurement (z angle) were recorded as predictor variables using a digital lateral cephalometric software (Carestream CS8100 SC) which were subjected to regression analysis using SPSS version 21.0 to develop a formula for age estimation. Results: Significant association on age was obtained for the two linear measurements. The regression formula generated for estimating the age was Age = 7.146 + 0.044 (AFH) + 0.146 (PFH) with R2 value = 0.674. Conclusion: Within the limitations of the present study, age estimation of teenagers in Salem population can be estimated. The predictability of the age can be increased by taking more cephalometric variables in generating the formula with increase in sample size.

Hyperplasia of the mandibular body: An anomaly in a developmental anomaly.

Hyperplasias of the mandible are usually seen in relation to the condyle or affecting one half of the mandible, such cases being described as hemimandibular hyperplasia or elongation. This article presents a rare case of hyperplasia of the right body of the mandible. The case being unique in that although being present from childhood did not cause any functional disturbances or any occlusal disharmony characteristically seen in such developmental anomalies. Here, we describe the clinical, radiographic and histopathologic findings that led to the diagnosis hyperplasia of the mandibular body and the treatment rendered to provide the esthetic correction.