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1.
J Appl Physiol (1985) ; 131(4): 1380-1389, 2021 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-34410849

RESUMO

Although the health benefits of exercise in adults with obesity are well described, the direct effects of exercise on adipose tissue that may lead to improved metabolic health are poorly understood. The primary aims of this study were to perform an unbiased analysis of the subcutaneous abdominal adipose tissue transcriptomic response to acute exercise in adults with obesity, and to compare the effects of moderate-intensity continuous exercise versus high-intensity interval exercise on this response. Twenty-nine adults with obesity performed a session of either high-intensity interval exercise (HI; 10 × 1 min at 90%HRpeak, 1 min recovery between intervals; n = 14) or moderate-intensity continuous exercise (MI; 45 min at 70%HRpeak; n = 15). Groups were well matched for BMI (HI 33 ± 3 vs. MI 33 ± 4 kg/m2), sex (HI: 9 women vs. MI: 10 women), and age (HI: 32 ± 6 vs. MI: 29 ± 5). Subcutaneous adipose tissue was collected before and 1 h after the session of HI or MI, and samples were processed for RNA sequencing. Gene set enrichment analysis revealed 7 of 21 gene sets enriched postexercise overlapped between HI and MI. Interestingly, both HI and MI upregulated gene sets involved in inflammation (IL6-JAK-STAT3 signaling, allograft rejection, TNFα signaling via NFκB, and inflammatory response; FDR q value < 0.25). Exercise also downregulated adipogenic and oxidative metabolism gene sets in both groups. Overall, these data suggest genes involved in subcutaneous adipose tissue metabolism and inflammation may be an important part of the initial response after a session of exercise.NEW & NOTEWORTHY This study compared the effects of a single session of high-intensity interval exercise versus moderate-intensity continuous exercise on transcriptional changes in subcutaneous abdominal adipose tissue collected from adults with obesity. Our novel findings indicate exercise upregulated inflammation-related gene sets, while it downregulated metabolism-related gene sets - after both high-intensity and moderate-intensity exercise. These data suggest exercise can alter the adipose tissue transcriptome 1 h after exercise in ways that may impact inflammation and metabolism.


Assuntos
Exercício Físico , Obesidade , Gordura Abdominal , Tecido Adiposo , Adulto , Feminino , Humanos , Inflamação/genética , Obesidade/genética , Gordura Subcutânea
2.
J Agric Food Chem ; 56(19): 9269-76, 2008 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-18783242

RESUMO

The structural characteristics of the gum exudate of Acacia senegal (gum arabic) have been investigated by monitoring the composition and physicochemical properties before and after treatment with proteolytic enzyme and various alkaline systems. Molecular mass ( M w) and radius of gyration ( R g) measurements were performed using gel permeation chromatography (GPC) coupled to refractive index, UV absorbance, and multiangle light scattering detectors and indicated that the macromolecules present have a compact structure. It was found that treatment with proteolytic enzyme caused the arabinogalactan-protein component (AGP) with average molecular mass approximately 2 x 10 (6) Da to degrade, yielding material of molecular mass approximately 4 x 10 (5) Da, whereas the bulk of the material corresponding to the protein-deficient arabinogalactan component (AG) with molecular mass 4 x 10 (5) remained unaffected. Barium hydroxide was found to hydrolyze the polysaccharide component (AG) itself in addition to the proteinaceous component as demonstrated in control experiments using dextran. However, sodium borohydride/sodium hydroxide treatments were unable to hydrolyze dextran and were assumed to hydrolyze only the proteinaceous component of gum arabic. The AGP component was completely degraded, yielding material of molecular mass approximately 4.5 x 10 (4) Da. It has been concluded, therefore, that the enzyme did not fully hydrolyze all of the protein present and that the AGP component of gum arabic consists of carbohydrate blocks of approximately 4.5 x 10 (4) Da linked to a polypeptide chain consistent with the wattle blossom structure. Because the AGP was degraded to differing extents using a mild and more severe sodium borohydride/sodium hydroxide treatment, it was concluded that the polysaccharide moieties were linked through both O-serine and O-hydroxyproline residues. The gum arabic sample was deglycosylated by treatment with anhydrous hydrogen fluoride and revealed the presence of two putative core proteins of approximately 3 x 10 (4) and approximately 5 x 10 (3) Da, respectively, which correspond to proteins of approximately 250 and 45 amino acids in length. A new model for the structure of the AGP component has been proposed.


Assuntos
Goma Arábica/química , Mucoproteínas/química , Aminoácidos/análise , Compostos de Bário , Glicosilação , Concentração de Íons de Hidrogênio , Hidrólise , Peso Molecular , Peptídeo Hidrolases/metabolismo , Proteínas de Plantas/química
3.
Plant Cell ; 13(10): 2283-95, 2001 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11595802

RESUMO

Transport of nucleotide sugars across the Golgi apparatus membrane is required for the luminal synthesis of a variety of plant cell surface components. We identified an Arabidopsis gene encoding a nucleotide sugar transporter (designated GONST1) that we have shown by transient gene expression to be localized to the Golgi. GONST1 complemented a GDP-mannose transport-defective yeast mutant (vrg4-2), and Golgi-rich vesicles from the complemented strain displayed increased GDP-mannose transport activity. GONST1 promoter::beta-glucuronidase studies suggested that this gene is expressed ubiquitously. The identification of a Golgi-localized nucleotide sugar transporter from plants will allow the study of the importance of this class of proteins in the synthesis of plant cell surface components such as cell wall polysaccharides.


Assuntos
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Proteínas de Transporte/genética , Complexo de Golgi/metabolismo , Proteínas de Membrana Transportadoras , Sequência de Aminoácidos , Arabidopsis/citologia , Arabidopsis/crescimento & desenvolvimento , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/metabolismo , Sequência de Bases , Proteínas de Transporte/química , Proteínas de Transporte/metabolismo , Primers do DNA , Glicosilação , Membranas Intracelulares/metabolismo , Microscopia Confocal , Dados de Sequência Molecular , Conformação Proteica , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos
4.
Plant Mol Biol ; 45(4): 421-35, 2001 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11352461

RESUMO

A cDNA corresponding to the core protein of an immunoaffinity-purified arabinogalactan protein (AGP) secreted aucus carota (carrot) cells in liquid culture was isolated. This cDNA, DcAGP1, encodes a new class of non-classical' AGP with strong similarity to a family of basic proline-rich proteins. The protein is rich in proline (17%), alanine (10%) and lysine (11%) and contains four distinct domains: a signal peptide, a proline-rich domain, a histidine-rich basic domain and a cysteine-containing 'PAC' domain that is found in a range of other cell wall proteins. The protein contains several sequence motifs found in otherwise unrelated cell wall proteins, but also displays some unique features. Northern blot analyses show that while the DcAGP1 transcript is abundant in the suspension-culture cells from which the AGP was obtained; in carrot seedlings the gene is only expressed at low levels in the roots and it is neither wound- nor stress-inducible. Furthermore, northern and western blot analyses demonstrate that the core polypeptide of DcAGP1 is differentially glycosylated in two different carrot suspension cultures. The unusual features of the protein sequence suggest that the DcAGP1 protein is a member of a family of basic proline-rich proteins defined by the C-terminal PAC domain, and the possible function(s) of the DcAGP1 protein is considered in the light of current views on AGP structure and function.


Assuntos
Daucus carota/genética , Mucoproteínas/genética , Peptídeos/genética , Sequência de Aminoácidos , Sequência de Bases , Northern Blotting , DNA Complementar/química , DNA Complementar/genética , DNA Complementar/isolamento & purificação , Daucus carota/citologia , Regulação da Expressão Gênica de Plantas , Dados de Sequência Molecular , Filogenia , Proteínas de Plantas , Plantas/genética , Domínios Proteicos Ricos em Prolina , RNA de Plantas/genética , RNA de Plantas/metabolismo , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos
5.
Food Addit Contam ; 13(8): 991-9, 1996.
Artigo em Inglês | MEDLINE | ID: mdl-8950118

RESUMO

The physiochemical and immunological properties of three Sudanese gum arabic samples and four gum tahla samples (two Sudanese, one West African and one Tanzanian--Acacia seyal var. seyal) were compared. The optical rotation (ca -30 degrees) and rhamnose (12-14%), arabinose (24-29%), galactose (36-42%), glucuronic acid (16-17%), nitrogen (0.327-0.365%) and protein (2.16-2.41%) contents of the gum arabic samples were consistent with the FAO (1990) specification for Acacia gum. In contrast the gum tahla samples had positive [alpha]D values (+45 degrees to +54 degrees), lower rhamnose (3-4%) and higher arabinose (41-45%) contents and lower nitrogen (0.147-0.175%), and hence protein (0.97-1.15%), contents. All of the gum arabic samples precipitated with beta-glucosyl Yariv reagent and hence were shown to contain arabinogalactan-protein(s) (AGPs), whereas in all but one of the gum tahla samples AGPs were not detected. The strong interaction of gum tahla with a monoclonal antibody known to recognize arabinose residues present in AGPs and arabinogalactans (AGs) was consistent with the observed higher levels of arabinose present in the gum tahla samples relative to the gum arabic samples. The data presented confirm that there are a number of physicochemical and structural differences between gum arabic (A. senegal gum) and gum tahla (A. seyal gum), and that a quick and simple immunological technique (immunodot blots) using an antiAGP/AG monoclonal antibody (MAC 207) could be used to screen for the presence of gum tahla in gum arabic consignments.


Assuntos
Excipientes/química , Aditivos Alimentares/química , Goma Arábica/química , Acacia , Anticorpos Monoclonais/efeitos dos fármacos , Excipientes/análise , Excipientes/farmacologia , Aditivos Alimentares/análise , Aditivos Alimentares/farmacologia , Goma Arábica/análise , Goma Arábica/farmacologia
6.
Plant Physiol ; 103(1): 115-123, 1993 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12231918

RESUMO

Arabinogalactan proteins (AGPs) are secreted or membrane-associated glycoproteins that have been operationally defined as binding to [beta]-glucosyl Yariv artificial antigen, being rich in arabinose and galactose, and containing high levels of alanine, serine, and hydroxyproline. Using an anti-AGP monoclonal antibody (MAC 207) bound to cyanogen bromide-activated Sepharose 4B, we have purified by immunoaffinity chromatography an extracellular AGP from the culture medium of suspension-cultured cells of carrot (Daucus carota). The apparent molecular mass of this highly glycosylated proteoglycan is 70 to 100 kD as judged by sodium dodecyl sulfate-polyacrylamide gels. Although its sugar analysis, [beta]-glucosyl Yariv binding, and high alanine, serine, and proline content are consistent with it being an AGP, the amino acid composition unexpectedly revealed this molecule to have no detectable hydroxyproline. This suggests that this glycoprotein is not a "classical" AGP, but represents the first example of a new class of hydroxyproline-poor AGPs. Deglycosylation of the AGP with anhydrous hydrogen fluoride revealed that the purified proteoglycan contains probably a single core protein with an apparent molecular mass of 30 kD. Direct visualization of the native AGP in the electron microscope showed ellipsoidal putative AGP monomers, approximately 25 nm by 15 nm, that showed a strong tendency to self assemble into higher-order structures. Upon desiccation, the glycosylated AGP formed paracrystalline arrays visible in the light microscope. Polarized Fourier transform infrared microspectroscopy of these arrays demonstrated a high degree of polarization of the sugar moieties under these conditions. These results put possible constraints on current models of AGP structure; a putative role for these novel AGPs as pectin-binding proteins is discussed.

7.
Plant J ; 2(5): 733-9, 1992 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-1302630

RESUMO

ptl1, a gene expressed specifically in pistil transmitting tissue of Antirrhinum flowers, encodes a protein with similarity to plant extensins. The protein is rich in proline (28%) and serine (9%) and contains several proline-rich repetitive amino acid motifs found in other extensin-like proteins. The presence of three consensus N-glycosylation sites indicates that it is probably glycosylated. RNA blots show that the ptl1 transcript is abundant in mature pistillar tissue but absent from immature flower buds and all other plant organs tested. In-situ localization of mRNA demonstrates that ptl1 expression is confined to the transmitting tissue of the style and stigma. The presence of a putative signal peptide at the N-terminus of the protein, taken together with the expression pattern, indicates that the ptl1 product may be secreted into the extracellular matrix of the transmitting tissue. The possible contributions of the ptl1 product to the physical properties of the transmitting tissue are considered in the light of current views on extensin structure and function.


Assuntos
Glicoproteínas/genética , Proteínas de Plantas/genética , Plantas/genética , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Hibridização In Situ , Dados de Sequência Molecular , Pólen , RNA Mensageiro/genética , RNA Mensageiro/isolamento & purificação , Sequências Repetitivas de Ácido Nucleico/genética , Reprodução , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Distribuição Tecidual
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