Evaluation and Comparison of Caries Excavation Efficacy of Three Different Burs: A Micro-computed Tomographic-assisted Study.

AIMS AND OBJECTIVES: The aim of this study was to evaluate the effectiveness of three different caries excavation methods using micro-computed tomography (micro-CT). MATERIALS AND METHODS: Fifteen freshly extracted human molar teeth with occlusal dentinal caries were selected. The teeth were sectioned longitudinally into two halves and were randomly divided into three groups (n = 10) depending on the caries removal technique: Group 1: tungsten carbide (TC) bur, Group 2: cerabur (CB), and Group 3: Excavus (EX) tipEX). A preoperative micro-CT scan of all the samples was taken. The caries excavation procedures were carried out followed by postoperative micro-CT scan. The preoperative and postoperative scans of each tooth were analyzed for caries removal effectiveness (CRE), mineral density (MD), and minimal invasiveness potential (MIP) using Avizo 9.4 software (Thermo Fisher Scientific, Germany). Statistical analysis was conducted by applying three-way analysis of variance and independent sample t-test using the Statistical Package for the Social Sciences software, version 20.0 (IBM Corporation, USA). RESULTS: Among the three groups, the TC group had the smallest RC/IC (residual caries/initial caries) ratio, highest mean MD at the cavity floor, and highest MIP. The EX group significantly had the highest RC/IC, lowest mean MD, and lowest MIP. Both the CRE and MIP parameters of CB group were acceptable (RC/IC = 0.08, mean MD = 1.09g/cm3, and MIP = 1.09). CONCLUSION: As compared with the three excavation methods, CBs can be considered as an alternative to TC burs because of its MIP and complete removal of infected carious dentin.

Stereomicroscopic Dye Leakage Measurement of Six Different Root Canal Sealers.

INTRODUCTION: Hermetic sealing of the root canal is the most desirable outcome of any root canal treatment, but almost always the filling of the root canal is defective, which is a multifactorial outcome. One such factor majorly influencing the obturation is the root canal sealer used. AIM: The present study was done for evaluating microleakage in different root canal sealers. MATERIALS AND METHODS: Sixty extracted human single rooted teeeth were used in this in-vitro study. Sealers tested for microleakage in this study were zinc oxide eugenol based sealer, Sealapex, AH Plus, MTA Plus, EndoRez, Endosequence BC. All the specimens were examined under stereomicroscope for microleakage and the obtained data were statistically analysed using One-way ANOVA test and Tukey's multiple comparision tests using the software GraphPad Prism 7.02. RESULTS: The Endosequence BC group showed the least dye leakage and the highest leakage was seen in Zinc oxide Eugenol based sealer. CONCLUSION: Bio ceramic salers being hydrophilic show better sealing ability compared to resin based and eugenol based sealers.

Effect of ultrasonic activation of photosensitizer dye temoporfin (Foscan) on antimicrobial photodynamic therapy: An ex vivo study.

AIM: The aim of this study is to evaluate the effectiveness of ultrasonic activation of photosensitizer (PS) drug Foscan in the elimination of endodontic pathogen from root canal system. MATERIALS AND METHODS: The minimal bactericidal concentration of "Foscan" was determined using disc diffusion and broth dilution method. Forty-eight extracted single-rooted premolars with periapical pathology were used for the study. After access opening, the first microbial sample was taken. The teeth were then randomly divided into four groups: Group 1: Chemomechanical debridement (CMD), Group 2: CMD and passive ultrasonic irrigation (CMD + PUI), Group 3: CMD and antimicrobial photodynamic therapy (aPDT), and Group 4: CMD and aPDT with activation by ultrasonics. After antimicrobial protocols, the second microbiological sample was collected. The samples were analyzed by multiplex polymerase chain reaction for the effectiveness of four antimicrobial protocols on Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas gingivalis, and Treponema denticola. STATISTICAL ANALYSIS USED: Wilcoxon signed-rank test and Mann-Whitney U-test with the significant level P < 0.05, using IBM SPSS statistics 20 version software. RESULTS: Foscan at concentration of 3.125 µg/ml was chosen for antimicrobial analysis. The prevalence of T. denticola, F. nucleatum, P. gingivalis, and P. intermedia was 79.17%, 70.84%, 70.83%, and 58.33%, respectively. Group 4 showed highest bacterial reduction of 99.51%, followed by Group 1 which showed bacterial reduction of 97.35%. CONCLUSIONS: Foscan can be used as an effective PS dye at low concentration with minimal tendency for tooth discoloration. Ultrasonic activation of PS dye facilitated better diffusion into dentinal tubules and biofilm obtaining greater bacterial reduction.

Variable permanent mandibular first molar: Review of literature.

INTRODUCTION: The success of root canal therapy depends on the locations of all the canals, thourough debridement and proper sealing. At times the clinicians are challenged with variations in morphology of root canal. This review article attempts to list out all the variations of permanent mandibular first molar published so for in the literature. MATERIALS AND METHODS: An exhaustive search was undertaken using PUBMED database to identify published literature from 1900 to 2010 relating to the root canal morphology of permanent first molar by using key words. The selected artcles were obtained and reviewed. RESULTS: Total ninty seven articles were selected out of which 50 were original article and forty seven were case reports. The incidence of third canal in mesial root was 0.95% to 15%. The incidence of three rooted mandibular first molar was 3% to 33%. Only ninety cases reported with c-shape canal configuration. Incidence of Taurodintism without congenital disorder was very rare. CONCLUSION: The root canal treatment requires proper knowlegde of variations in root canal morphology in order to recognise, disinfect and seal all portal of exit. This can be accomplished with proper diagnosis using newer modes, modification in access preparation, use of operating microscope, enhanced methods of disinfecting and sealing of all canals.