RESUMO
The effect and contribution of an external magnetic field (MF) on the uptake and translocation of nanoparticles (NPs) in plants have been investigated in this study. Barley was treated with iron oxide NPs (Fe3O4, 500 mg/L, 50-100 nm) and grown under various MF strengths (20, 42, 125, and 250 mT). The root-to-shoot translocation of NPs was assessed using a vibrating sample magnetometer (VSM) and inductively coupled plasma optical emission spectrometry (ICP-OES). Additionally, plant phenological parameters, such as germination, protein and chlorophyll content, and photosynthetic and nutritional status, were examined. The results demonstrated that the external MF significantly enhances the uptake of NPs through the roots. The uptake was higher at lower MF strengths (20 and 42 mT) than at higher MF strengths (125 and 250 mT). The root and shoot iron (Fe) contents were approximately 2.5-3-fold higher in the 250 mT application compared to the control. Furthermore, the MF treatments significantly increased micro-elements such as Mn, Zn, Cu, Mo, and B (P < 0.005). This effect could be attributed to the disruption of cell membranes at the root tip cells caused by both the MF and NPs. Moreover, the MF treatments improved germination rates by 28%, total protein content, and photosynthetic parameters. These findings show that magnetic field application helps the effective transport of magnetic NPs, which could be essential for NPs-mediated drug delivery, plant nutrition, and genetic transformation applications. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03727-4.
RESUMO
The magnetic field (MF) interacts with biological systems and has the potential to increase germination, plant growth and productivity. Although it is known as a low cost and promising approach, the mechanism that increases growth is not fully understood yet. In this study, the effect of different MF strengths (20, 42, 125, and 250 mT) was investigated on barley (Hordeum vulgare L.). In addition to phenological parameters, possible cell damage, electron transport rate, chlorophyll fluorescence, magnetic character and elemental status of tissues were determined. Results showed that lower strengths (≤125 mT) of MF treatment improve germination. Confocal microscopy analyzes revealed MF-induced cell membrane damage in roots that could alter the elemental content of tissues. Elemental analyzes found that the content of macroelements (Ca, Mg, P, and K) are gradually reduced with increasing MF forces; in opposite the microelement contents (Fe, B, Cu, Mn, Zn, and Mo) are increased in roots. Diamagnetism is the dominant magnetic character in all root and leaf samples. However, the roots became surprisingly superparamagnetic in 250 mT application. It seems that MF treatment at higher strength (250 mT in this study) could influence the orientation of magnetic moments. These findings suggest that MF application: i) can alter the magnetic character of plants, ii) enhances the germination, photosynthetic machinery, and growth, and iii) affects the nutrient uptake and abundance in tissues, depending on the MF strength. This comprehensive study can help in understanding the interaction of magnetic field with plants.
