Maternal hepatitis B status and Sex at birth: A cross-sectional study in a Ghanaian population.

Maternal carrier status of hepatitis B has been associated with excess sons while maternal immunity to it has been associated with excess daughters at birth. However, the proportion of males at birth (sex ratio) is relatively low in Sub-Saharan Africa despite the relatively high prevalence of hepatitis B. However, no known study has tested this hypothesis in the Ghanaian population; hence the aim of the study. The study was cross-sectional between January and September 2023 at the Tamale Central Maternal and Child Health unit. The study involved 380 mothers of whom mothers with daughters (MD) were 145 (38.2 %) while the rest were mothers with sons (MS). The mothers were aged between 18 and 43 years and were sampled within one week of delivery to singleton births. Maternal venous blood samples were collected and tested for hepatitis B surface antigen (HBsAg), surface antibody (HBsAb), envelop antigen (HBeAg) envelope antibody (HBeAb) and core antibody (HBcAb) using immunochromatographic technique and total testosterone (TT), using ELISA. There was no significant difference in the serum total testosterone level between MD and MS (0.32 ± 0.13 vs 0.32 ± 0.27, P = 0.991). Moreover, while the mothers were seropositive for HBsAg (10.5 %), HBsAb (35.5 %), HBeAg (0.0 %), HBeAb (5.3 %) and HBcAb (11.8 %), there was no significant association between sex at birth and maternal hepatitis B status for HBsAg (ꭓ2: 0.531, P = 0.472), HBsAb (ꭓ2: 2.655, P = 0.140), HBeAb (ꭓ2: 0.251, P = 0.633) and HBcAb (ꭓ2: 0.101, P = 1.000). Maternal hepatitis B status may not be associated with the offspring sex at birth in the studied population from Ghana.

Exploring Hematological and Biochemical Disparities in Same-Sex and Opposite-Sex Females: A Cross-Sectional Twin Study in a Ghanaian Population.

There are sex-dependent differences in hematological and biochemical variables in adulthood attributed to the predominant effects of testosterone in males and estrogen in females. The Twin Testosterone Transfer (TTT) hypothesis proposes that opposite-sex females may develop male-typical traits due to exposure to relatively higher levels of prenatal testosterone than same-sex females. Additionally, prenatal testosterone exposure has been suggested as a correlate of current circulating testosterone levels. Consequently, opposite-sex females might exhibit male-typical patterns in their hematological and biochemical variables. Despite this hypothesis, routine laboratory investigations assign the same reference range to all females. Our cross-sectional study, conducted in Tamale from January to September 2022, included 40 twins, comprising 10 opposite-sex (OS) males (25%), 10 OS females (25%), and 20 same-sex (SS) females (50%), all aged between 18 and 27 years. Fasting venous blood samples were collected and analyzed using automated hematology and biochemistry laboratory analyzers. Results indicated that levels of hemoglobin, serum creatinine, gamma-glutamyl transferase, total protein, globulins, and total testosterone were significantly higher in OS males than OS females. Conversely, total cholesterol and low-density lipoprotein cholesterol were significantly higher in OS females than OS males. Unexpectedly, levels of low-density lipoprotein cholesterol and total testosterone were significantly higher in SS females than OS females. Contrary to expectations, opposite-sex females did not exhibit male-typical patterns in their hematological and biochemical variables. This suggests that the TTT effect may not occur or may not be strong enough to markedly affect hematological and biochemical variables in OS females.

Immune checkpoint molecules B7-1 and B7-H1 as predictive markers of pre-eclampsia: A case-control study in a Ghana.

BACKGROUND/AIM: Immune tolerance in the fetal-maternal junction is maintained by a balance in the Th1/Th2 system. Th1-type immunity is associated with pro-inflammatory cytokines and immune checkpoint molecules (ICMs) such as B7-H1, while Th2-type immunity is characterized by anti-inflammatory cytokines and ICMs such as B7-1. Any imbalance in the Th1/Th2 immune system may lead to adverse pregnancy outcomes such as pre-eclampsia (PE). Hitherto, the potential of serum B7-1 and B7-H1 proteins as early markers of PE has not been explored in the Ghanaian population. MATERIALS AND METHODS: This was a case-control study from May 2020 to April 2022 at the War Memorial and the Upper East Regional Hospitals. The study involved 291 women, including 180 (61.9%) with normotensive pregnancy and 111 (38.1%) with PE. Venous blood samples were collected and assayed for blood cell count, serum interleukins (ILs)-4, -6, -12, -18, and TNF-α as well as serum B7-1 and B7-H1 proteins. RESULTS: The monocyte count (p = .007), the serum levels of IL-18 (p = .035), TNF-α (p = .001), and B7-H1 (p = .006) were significantly higher in PE than in normotensive pregnancy. In addition, the monocyte count (p = .002), the serum levels of IL-12 (p = .029), TNF-α (p = .016), and B7-1 (p = .009) levels were significantly higher in the third trimester than the second trimester PE. In predicting PE, the area under the curve of cytokines and ICMs ranged from 0.51 for IL-6 to 0.62 for TNF-α. CONCLUSION: PE may be characterized by a dominant Th1-type immunity with higher levels of pro-inflammatory cytokines and B7-H1 proteins, but these variables may not be suitable for predicting PE.

The impact of age on sex bias in models for height and sex estimation based on hand and foot dimensions the impact of age on sex bias.

There are sex differences in age-related bone modifications after puberty. Androgens stimulate radial bone expansion in males, while estrogen stimulates endosteal apposition but limits periosteal expansion in females. The potential effect of age on observed sex biases in height and sex estimation models is most significantly relevant for forensic or bioarchaeological research that relies, at least in part, on hand and foot bone measurements of living or skeletal remains for purposes of identification or demographic reconstruction. This study sought to determine whether age affects sex biases in models for height and sex estimation which are based on hand and foot dimensions. The study was cross-sectional between January and June 2021 at the University for Development Studies. The study included 379 participants (male = 161 and female = 218) between 20 and 29 years. The hand length (HL), hand width (HW), foot length (FL) and foot width (FW) were measured twice from the left side using computer-assisted analysis. Univariable and multivariable discriminant and linear regression models were formulated for sex and height estimation respectively. Females were better classified than males with sex biases (male-female) ranging from -1.3% to -22.6% for all models. Models for height estimation were more precise in males (bias: 0.0-0.3 cm) than in females (bias: 0.3-1.4 cm). However, age did not have an impact on the observed sex biases. Height and sex estimation from foot and height dimensions may not need adjustment for age. This may, however, be limited to a given population and age group.

Are sex differences in blood cell count and hemoglobin moderated by the 2D:4D ratio? A cross-sectional study in a Ghanaian population.

Background and Aims: There are sex differences in blood cell count and hemoglobin (HGB) in adulthood due to differences in the levels of circulating sex hormones. The second-to-fourth digit ratio (2D:4D) is the putative marker of prenatal hormone exposure. The 2D:4D or the right-left difference (Dr-l) are sexually dimorphic and are correlates of sex hormones in adulthood. The study sought to determine whether sex differences in adult blood cell count and HGB can be partly explained by the 2D:4D or Dr-l. Methods: The study was cross-sectional between June and December 2021 at the University for Development Studies. The study involved 207 healthy participants (females = 113) aged from 18 to 32 years. The right-hand (2D:4DR), and the left-hand (2D:4DL) digit ratio and their difference (Dr-l) were measured using Computer-assisted analysis. Blood cell count, HGB, testosterone, and estradiol were measured from venous blood samples using an automated HGB analyzer and ELIZA technique. Results: The platelet count was inversely related to the 2D:4DR in the total sample with the 2D:4DR accounting for about 0.2% (adjR 2 = 0.002) of the variability in platelet count. However, there was a sex difference as indicated by the significant interaction between sex and the 2D:4DR on platelet count (p = 0.03). The relationship between platelet count and the 2D:4DR was negative in females but positive in males. Also, there was a positive relationship between HGB concentration and the Dr-l in the total study sample, where the Dr-l accounted for about 0.6% (adjR 2 = 0.006) of the variability in HGB concentration. Sex interacted significantly with the Dr-l on HGB concentration (p = 0.01) such that the relationship between HGB and the Dr-l was positive in females but negative in males. Conclusion: Prenatal hormone exposure, as indexed by the 2D:4D ratio, may partly account for the observed sex differences in platelet count and HGB levels in adulthood.

Can maternal postpartum testosterone and estradiol retrospectively predict the offspring's sex at birth? A cross-sectional study in Ghana.

The selection of X- or Y-bearing spermatozoa during fertilization may depend on maternal circulating sex hormones. The zona pellucida of the developing oocyte is adapted to be selective for the Y-bearing spermatozoa when maternal circulating androgens are relatively high. This study sought to determine whether maternal postpartum testosterone and estradiol can retrospectively predict the offspring sex at birth. The study was cross-sectional from December 2020 to April 2021 at the Reproductive and Child Health unit in Tamale. The participants were part of a previous study and comprised 178 mother-offspring dyads (mother-daughter = 90, mother-son = 88). The mothers were between the ages of 18 and 35 years and had a median (interquartile range-IQR) postpartum interval of 111 (60-187) days. A single venous blood sample was drawn from the mothers between 8.00 am and 12.00 pm local time on each day to reduce diurnal variation. Postpartum serum estradiol, testosterone, and sex hormone-binding globulin were assayed using the ELISA technique. The serum total testosterone and the testosterone-to-estradiol ratio (TT: E2 ) were higher in mothers with sons while estradiol was higher in mothers with daughters (p < 0.050). The total testosterone and TT: E2 did not markedly differ by their area under the curve (AUC: 0.91 and 0.99, respectively) but both were higher than the AUC of estradiol (0.72). The Sensitivity was 97.7%, 97.7%, and 94.5% and specificity, 88.9%, 40.0%, and 95.5% at cutoff points of >1.659 nmol/L, ≤141.862 pmol/L, and > 31.5, respectively for total testosterone, estradiol, and TT: E2 . The maternal testosterone-to-estradiol ratio may be more predictive of offspring sex at birth than either testosterone or estradiol alone.

Insulin Resistance in relation to Hypertension and Dyslipidaemia among Men Clinically Diagnosed with Type 2 Diabetes.

Pathophysiologically, type 2 diabetes can result from insulin resistance or insulin insufficiency alone. It is unclear whether relative insulin shortage or pronounced insulin resistance is linked to poor cardiometabolic problems like obesity. Therefore, the objective of this study was to evaluate the relationship between insulin resistance (IR), hypertension, and dyslipidaemia, in men with type 2 diabetes mellitus. One hundred and twenty-one (121) type 2 diabetic men participated in this cross-sectional study, which was conducted between September 2018 and September 2019. Sociodemographic information was collected using a self-designed questionnaire. Anthropometric data were also taken and blood samples collected for estimation of insulin, glucose, and lipid concentrations. HOMA-IR was calculated from the fasting insulin and glucose values, and a HOMA - IR ≥ 2 was considered to indicate insulin resistance. Of the 121 participants, 39.7% were classified as insulin-resistant. Levels of total cholesterol (4.82 ± 1.2 mmol/L; p = 0.007 vs. 4.25 ± 1.1 mmol/L), LDL cholesterol (3.17 ± 0.9 mmol/L; p = 0.001 vs. 2.52 ± 0.8 mmol/L), and TC/HDL-C ratio (3.93 ± 0.9; p = 0.042 vs. 3.58 ± 0.9) and the prevalence of abnormal LDL-C (14.6%; p = 0.015 vs. 2.7%) and elevated BP (83.3%; p = 0.048 vs. 67.1%) were higher in the insulin-resistant group. LDL cholesterol (AUC = 0.670; p = 0.001) better classified subjects as being insulin-resistant compared to other lipid markers. The odds of insulin resistance in dyslipidaemia were not statistically significant after adjusting for obesity. The link between insulin resistance and dyslipidaemia and hypertension in male diabetics may thus be mediated by obesity.

Interactions between sex and the age at disease onset on cardiometabolic risk factors in a Ghanaian population with type 2 diabetes mellitus: A cross-sectional study.

Background and Aim: The are sex differences in cardiometabolic risk factors in type 2 diabetes mellitus (T2DM) as well as the age at disease onset. However, the impact of these risk factors on the age at onset of T2DM is less known in the Ghanaian population. An understanding of the differential impact of cardiometabolic risk factors on the age at onset on T2DM may lead to sex-specific interventions in preventive and management strategies for T2DM. Methods: The study was cross-sectional from January to June 2019 at the Bolgatanga regional hospital. The study involved 163 T2DM patients (Female = 103, Male = 60), aged from 25 to 70 years. The body mass index (BMI) and the waist-to-hip ratio (WHR) were measured following standardized anthropometric techniques. Fasting venous blood samples were collected and analyzed for cardiometabolic risk factors including total cholesterol (TCHOL) and low-density lipoprotein (LDL) cholesterol. Results: While TCHOL was higher in males than females (mean [SD]: F = .78 [1.37], M = 4.27 [1.39]) and LDL higher in females than males (mean [SD]: [F = 4.33 [1.22], M = 3.87 [1.26]), these did not, however, attain conventional statistical significance for TCHOL (t = 1.985, p = 0.05) and LDL (t = 2.001, p = 0.05). There were however, significant interactions between sex and the age at disease onset on TCHOL (t = -2.816, p = 0.006) and LDL (t = -2.874, p = 0.005), which were independent of the BMI, WHR and disease duration. The relationship between the age at disease onset and that of TCHOL and LDL were positive in females but negative in males. Conclusion: Fasting plasma TCHOL and LDL increases with increasing age at onset of T2DM in females but decreases in males. Strategies for the prevention and management of T2DM should be sex-specific. Females with T2DM should be given more attention regarding their fasting plasma cholesterol (total) and LDL cholesterol as they are more likely than men to have increased levels of these lipids with increasing age at disease onset.

Sex-moderated relationship between the 2D:4D ratio and circulating hormones in an adult Ghanaian population.

The second-to-fourth digit ratio (2D:4D) is the putative marker of prenatal hormone exposure. The 2D:4D ratio or the right-left difference (Dr-l) are said to be negative and positive correlates, respectively, of circulating testosterone and estrogen in both adult males and females. However, previous studies on the subject have reported mixed results. This study aimed to determine the sex-moderated relationship between the 2D:4D ratio and adult circulating testosterone, estradiol, testosterone-to-estradiol ratio and the free androgen index. This was a cross-sectional study from January to June 2021 at the University for Development Studies, Ghana. The study involved 62 participants (Female = 28; Male = 34), aged between 20 and 26 years. The right (2D:4DR), the left (2D:4DL), and their difference (Dr-l) were measured by computer-assisted analysis. Fasting venous samples were assayed for total testosterone (T), estradiol (E2 ), and sex hormone-binding globulin (SHBG) using ELISA. The free androgen index (FAI) was then calculated (T/SHBG) and the data were analyzed using moderated and/or weighted regression. Males had significantly higher T and FAI than females while females had significantly higher E2 than males, which were independent of age and body mass index (p < 0.001). There was a significant SEX*Dr-l interaction on FAI (p = 0.007). The Dr-l correlated negatively with FAI in males but positively in females and accounted for about 94.0% of the variability of FAI in males (adjR2  = 0.940) and only 0.2% in females (adjR2  = 0.002). The 2D:4D ratio, a putative marker of prenatal hormone exposure, may have an impact on sex differences in adult free androgen index.

Does sex interact with the 2D:4D ratio or adult circulating hormones on the estimated glomerular filtration rate? A cross-sectional study in Ghana.

The 2D:4D ratio is the putative marker of prenatal hormone exposure and has been suggested as a correlate of adult circulating testosterone and estrogen. The study aimed to determine whether sexual dimorphism in the estimated glomerular filtration rate (eGFR) can be partly explained by the 2D:4D ratio or adult circulating testosterone or estrogen. The study was cross-sectional from June to December 2021 at the University for Development Studies. The study involved 206 healthy adults (Female = 93, Male = 113) between 18 and 30 years. The 2D:4D ratio was measured using computer-assisted analysis. Venous blood samples were collected and analyzed for testosterone, estradiol and creatinine using the ELISA technique and routine biochemical analysis. The adjusted eGFR was calculated using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) creatinine equation (2021). The eGFR and the testosterone-to-estradiol ratio (TT:E2 ) were significantly higher in males than in females (p < 0.001). There was a significant interaction between sex and the TT:E2 on the eGFR (p < 0.001). Although the relationship between the eGFR and the TT:E2 was negative in both males and females, a unit change in the TT:E2 had a greater impact on the eGFR in females (B = -1.38) than in males (B = -0.01). Sexual dimorphism in the eGFR is influenced by both testosterone and estradiol. Although the sex difference in the eGFR may be influenced by the TT:E2 , estrogen seems to account for more variability in the eGFR than testosterone.

Sexual Dimorphism and Sex-2D : 4D Interactions on Fasting Lipid Variables in an Adult Ghanaian Population.

Prenatal hormone exposure has been suggested as a correlate of adult circulating estrogen and testosterone. If this observation is true, then prenatal hormone exposure may have an association with lipid homeostasis in adulthood. The study sought to investigate sexual dimorphism and the interactions between the putative marker of prenatal hormone exposure (2D : 4D) and sex on adult fasting plasma lipid variables. The study was cross-sectional from June to December 2021 at the University for Development Studies. The participants were between 18 and 30 years of age and consisted of 206 healthy persons (female = 93, male = 113). The right hand (2D : 4DR), the left hand (2D : 4DL), and the right-left 2D : 4D difference (Dr-l) were measured using computer-assisted analysis. Fasting venous blood samples were collected and analyzed for lipid variables including total cholesterol (TCHOL) and high-density lipoprotein cholesterol (HDL-C). There were no significant differences in the 2D : 4D ratio and lipid variables between males and females. However, after adjusting for age and BMI, the 2D : 4DR (P = 0.014) and the 2D : 4DL (P = 0.007) increased with increasing fasting plasma HDL-C on average. Moreover, there were significant interactions between sex and the 2D : 4DR (P = 0.002) and also, the 2D : 4DL (P = 0.005) on fasting plasma HDL-C. The relationship between HDL-C and the 2D : 4D ratio was positive in females but negative in males. The 2D : 4DR accounted for about 54.9% and 46.0% while the 2D : 4DL accounted for about 48.2% and 14.0% of the variabilities in fasting plasma HDL-C in females and males, respectively. Prenatal hormone exposure may partly account for the sexual dimorphism in adult lipid homeostasis.

The association between the 2D:4D ratio and offspring sex at birth: A cross-sectional study in Ghana.

OBJECTIVES: The 2D:4D ratio is the putative marker of prenatal androgen exposure. Low maternal 2D:4D ratio has been associated with high or male-biased secondary sex ratio. Hitherto, there has not been any study in Ghana regarding the maternal 2D:4D ratio and sex ratio at birth. This study sought to investigate this observation in a Ghanaian population. METHOD: The study was cross-sectional from December 2020 to April 2021 involving 272 first-time mothers. The mean ± SD age of the mothers was 23.9 ± 3.67 years. The right (2D:4DR), the left (2D:4DL), the mean (M2D:4D) digit ratios and the right-left difference (Dr-l) of the mothers were measured using computer-assisted analysis. The mothers were stratified by their digit ratios, and the cohort sex ratio (CSR) for each stratum was then calculated as the proportion of sons. RESULTS: The mean ± SD of the 2D:4DR of mothers-with-daughters and mothers-with-sons were 0.941 ± 0.032 and 0.933 ± 0.037, respectively. The mean ± SD of the 2D:4DL of mothers-with-daughters was 0.934 ± 0.034, while that of mothers-with-sons was 0.931 ± 0.039. The offspring sex at birth and the CSR was not associated with either the mother's right, left, mean 2D:4D ratio or their difference (Dr-l). However, mothers-with-daughters showed rightward asymmetry in their 2D:4D ratios as the Dr-l significantly and positively deviated from zero (p < .010). CONCLUSION: The maternal 2D:4D ratio may not be associated with their offspring sex at birth among first-time mothers. This study adds to the limited data on studies regarding offspring sex at birth and the 2D:4D ratio in Ghana and Sub-Saharan Africa.

The relationship between offspring's 2D:4D ratio and postpartum maternal circulating testosterone, estradiol, and their indices in a Ghanaian population.

OBJECTIVES: The 2D:4D ratio is influenced by prenatal testosterone (PT) and estrogen (PE) exposure in utero. This study sought to determine whether evidence of Manning's hypothesis can still be observed even in the postpartum period. We hypothesize that the offspring 2D:4D ratios will be inversely correlated with maternal postpartum circulating testosterone but positively correlated with estradiol. METHODS: This study was conducted between December 2020 and April 2021 and was cross-sectional in nature. There were 272 mother-offspring pairs; the mothers were aged between 18 and 36 years while the median (IQR) age of their offspring was 111 (44-210) days. Offspring right (2D:4DR) and left (2D:4DL) digit ratios were measured using computer-assisted analysis. Sampling was done at 111 (44-210) days postpartum and blood was analyzed for total testosterone (TT), estradiol (E2) and sex hormone-binding globulins using the enzyme-linked immunosorbent assay technique. RESULTS: The 2D:4DR of sons was significantly lower compared to daughters (p = .031). Mothers with sons had significantly increased levels of serum TT (p = .001) while mothers with daughters had significantly increased levels of E2 (p = .000). As hypothesized, the maternal serum free testosterone (FT%) was inversely correlated with their daughters' (r = -0.320, p = .003), and also with their sons' (r = -0.213, p = .047), 2D:4DL. Unexpectedly, daughters' 2D:4DL was inversely correlated with maternal circulating free E2 (r = -0.255, p = .015). CONCLUSIONS: In humans, evidence of the relationship between maternal testosterone levels and their offspring's 2D:4D ratio may persist even into the postpartum period.

The relationship between 2D:4D ratio and postpartum adult female variables in a Ghanaian population.

OBJECTIVES: Postpartum hematological and anthropometric assessment is a requirement for optimal maternal and child health. The study aimed to determine the relationship between the 2D:4D ratio and postpartum hematological and anthropometric variables in adult females. METHODS: The study was cross-sectional from December 2020 to April 2021 involving 272 postpartum adult females, aged between 18 and 36 years. The right (2D:4DR) and the left (2D:4DL) digit ratios were measured using computer-assisted analysis. Fasting venous samples were collected at a median (interquartile range) of 111 (44-210) days postpartum and analyzed for total testosterone (TT), estradiol, sex hormone-binding globulin, and complete blood count. RESULTS: The mean ± standard deviation 2D:4DR and 2D:4DL were 0.94 ± 0.04 and 0.93 ± 0.04, respectively. As expected, the TT (r = -0.198, p = .015) and the free androgen index (FAI: r = -0.186, p = .019) were inversely correlated with the 2D:4DL while free testosterone (FT%: r = -0.157, p = .038) was inversely correlated with the 2D:4DR. The absolute basophile count (BASO: r = -0.124, p = .040) and the Platelet-lymphocyte ratio (PLR: r = -0.153, p = .016) were inversely correlated with the 2D:4DL and the 2D:4DR respectively. In addition, the mean cell volume was inversely correlated with the 2D:4DR (r = -0.139, p = .024) and the 2D:4DL (r = -0.122, p = .045). Moreover, the 2D:4DR was inversely correlated with height (r = -0.164, p = .007). Unexpectedly, the red blood cell count (RBC: r = 0.138, p = .025) was positively correlated with the 2D:4DR. CONCLUSION: There are significant relationships between the 2D:4D ratio and postpartum female variables. These findings are useful preliminary reference data for postpartum research and subsequent 2D:4D ratio studies.

Second to fourth (2D:4D) digit ratio and their relationships among a mother and child population in Ghana.

The study aimed to determine the relationship between digit ratios among a mother-child population in Ghana. This was a cross-sectional study from December 2020 to April 2021 involving 272 mothers, their daughters (n = 132) and their sons (n = 140). The right (2D:4DR) and the left (2D:4DL) digit ratios were measured using computer-assisted analysis. The data were analysed in SPSS (v23) and GraphPad Prism (v8) at an alpha value of 0.05. The mean ± SD age of the mothers was 23.9 ± 3.67 years while the median (IQR) age of daughters was 116(54-240) days and sons, 134(54-240) days. The mean ± SD 2D:4DR were 0.94 ± 0.04, 0.91 ± 0.04 and 0.90 ± 0.04 respectively for mothers, daughters and sons. The mean ± SD 2D:4DL was 0.93 ± 0.04, for mothers, 0.92 ± 0.05 for daughters and 0.92 ± 0.05 for sons. The daughters and sons showed leftward asymmetry while the mothers showed rightward asymmetry in digit ratios. The 2D:4DR of sons was significantly lower than daughters (P = 0.031). There were negative correlations between the 2D:4DL and age of daughters (r = -0.182, P = 0.043) and sons (r = -0.221, P = 0.012). The 2D:4DR of mothers was positively correlated with that of daughters (r = 0.332, P = 0.000) and that of sons (r = 0.233, P = 0.008). There are significant relationships between digit ratios in a mother-child population.

Single nucleotide polymorphisms in LCAT may contribute to dyslipidaemia in HIV-infected individuals on HAART in a Ghanaian population.

Highly active antiretroviral therapy (HAART) is known to cause lipid abnormalities such as dyslipidaemia in HIV-infected individuals. Yet, dyslipidaemia may not independently occur as it may be worsened by single nucleotide polymorphisms (SNPs) in lecithin cholesterol acyltransferase (LCAT) and lipoprotein lipase (LPL). This case-control study was conducted in three-selected hospitals in the Northern part of Ghana. The study constituted a total of 118 HIV-infected participants aged 19-71 years, who had been on HAART for 6-24 months. Dyslipidaemia was defined based on the NCEP-ATP III criteria. HIV-infected individuals on HAART with dyslipidaemia were classified as cases while those without dyslipidaemia were grouped as controls. Lipid profile was measured using an automatic clinical chemistry analyzer and genomic DNA was extracted for PCR (GeneAmp PCR System 2700). Overall, the prevalence of dyslipidaemia was 39.0% (46/118). High levels of low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), and reduced levels of high-density lipoprotein cholesterol (HDL-C) were observed in all cases. A total of 256 selected PCR amplicons comprising 137 LPL (exons 3, 5 and 6) and 119 LCAT (exons 1, 4, and 6) were sequenced in 46 samples (Inqaba Biotech). Six (6) clinically significant SNPs were identified in exons 1 and 4 for LCAT whereas 25 non-clinically significant SNPs were identified for LPL in exons 5 and 6. At position 97 for LCAT exon 1, there was a deletion of the nucleotide, 'A' in 32.5% (13/40) of the sampled population while 67.5% (27/40) of the sample population retained the nucleotide, 'A' which was significantly associated with dyslipidaemic outcomes in the study population (p = 0.0004). A total of 25 SNPs were identified in exons 5 and 6 of LPL; 22 were substitutions, and 3 were insertions. However, none of the 25 SNPs identified in LPL exon 5 and 6 were statistically significant. SNPs in LCAT may independently contribute to dyslipidaemia among Ghanaian HIV-infected individuals on HAART, thus, allowing genetic and/or functional differential diagnosis of dyslipidaemia and creating an opportunity for potentially preventive options.

Forensic science in Ghana: A review.

The use of forensic science continues to grow across the world. In Ghana, major advancements took off in 2011, including the introduction of modern DNA profiling and the establishment of an automated fingerprint identification system. These developments have led to some positive impacts on the delivery of justice, including the exoneration of a wrongly incarcerated individual. However, a review of the policy-related aspects of forensic science shows gaps in legislation, governance, service provision, quality assurance and accreditation, education and research. An important recommendation to improve forensic science in Ghana is the creation of a "national policy strategy", a blueprint informed by relevant stakeholders, best practice from other countries and the status of the field. Resolutions to the policy issues identified in this review will ensure a more robust application of forensic science in delivering safe justice and enhancing public security.