The All4Children project to assess the initial implementation of the Integrated Model of Family Foster Care in Portugal: A description of the study protocol.

BACKGROUND: The All4Children project addresses the urgent need to transition from institutionalization to family-based care for out-of-home children in Portugal. Despite evidence highlighting the detrimental effects of institutionalization, only a small percentage of children (less than 4%) are currently placed in family foster care in the country. In response to European directives for deinstitutionalization, Portuguese legislation now prioritizes non-kinship family foster care as the preferred alternative for young children in need of care. To facilitate this transition, the Integrated Model of Family Foster Care (MIAF) was developed, offering a comprehensive framework covering the entire spectrum of family foster care. OBJECTIVE: This research aims to investigate the initial implementation stage of the MIAF to promote high-quality family foster care in Portugal. METHOD: The study will conduct a mixed-method and longitudinal research project in family foster care agencies across different regions of Portugal, focusing on evaluating the implementation and outcomes of the MIAF model using a multi-informant and multi-method approach. The participants will include caseworkers, children aged 0-9 years entering foster care, and their respective foster families enrolled in the MIAF program. Process evaluation will assess fidelity, feasibility, appropriateness, and acceptability of MIAF modules, while outcome evaluation will examine child safety, stability, well-being, as well as foster family well-being and quality of relational care. OUTCOMES: The insights gained from this research initiative will serve as a foundation for the ongoing enhancement of MIAF. Consequently, this project has the capacity to advance evidence-based child welfare practices by refining processes and strategies to better serve vulnerable children and youth. CONCLUSION: Facilitated by a multidisciplinary team, this project will contribute to advancing research in the field, enhancing practice, and informing policy during a pivotal stage of deinstitutionalization in Portugal.

How does sensitivity influence early executive function? A critical review on hot and cool processes.

There is compelling evidence that the quality of caregiver-child interactions during toddlerhood and the preschool years supports the development of executive function (EF) (Bernier et al., 2010; 2015; 2016; Fay-Stammbach et al., 2014; Geeraerts et al., 2021). Based on such findings, we make the case herein that sensitivity may be one of the most important dimensions of parenting contributing to early EF. In the present article, we will review empirical evidence, integrating findings from a wide range of scientific disciplines - cognitive psychology, neuroscience, and developmental psychopathology - and present theoretical ideas about how two contexts of sensitive caregiving - i.e. sensitivity to distress and non-distress cues - may be contributing differently to hot and cool EF development. Implications for future investigations on the environmental contributors of early EF, and its mechanisms, are discussed.

Cortisol reactivity and negative affect among preterm infants at 12 months during a mother-infant interaction task.

The purpose of this study was to investigate correlates of preterm (PT) infant's cortisol reactivity and the association to infant negative affect, during a mother-infant interaction procedure. Participants included 48 infants born prematurely (gestational age < 37 weeks) and their mothers, assessed when infants were 12 months old corrected for prematurity. The examined variables comprised both neonatal and environmental dimensions including maternal interactive behavior. Infant negative affect and maternal interactive behavior were assessed with a standardized mother-infant interaction task. A baseline infant saliva sample was collected before the interaction began, and a second sample after the interaction episodes ended. Results revealed that decrease of infant's cortisol concentration was significantly associated with the exposure to more sensitive, and less intrusive maternal behaviors. However, once controlled for neonatal risk, family SES and maternal psychological distress, the associations were rendered non-significant. Although the association between cortisol reactivity and negative affect trended toward significance, maternal intrusiveness was the only significant predictor of observed infant negative affect. Findings suggest the importance of primary relational experiences on PT infants' early regulatory competencies.

Emotional and Behavioral Health among Portuguese Toddlers during the COVID-19 Crisis: The Impact of Social Isolation and Caregiving Distress.

The COVID-19 pandemic has led to significant changes in the lives of families with young children. The present study aimed to explore whether child social isolation due to the COVID-19 crisis was associated with toddlers' emotional and behavioral health (EBH) and whether this association was moderated by caregiving distress, during the second mandatory lockdown in Portugal. Participants included 315 toddlers and their primary caregivers. Caregivers were invited to complete a set of questionnaires in order to report about toddlers' social isolation from other significant family members, other children, and activities outside the house, and to provide ratings of caregiving distress and toddlers' EBH. Family socioeconomic factors, including stressors resulted from the pandemic, were also measured. Significant interaction effects, independent of child sex and sociodemographic factors, between COVID-19-related social isolation and caregiving distress emerged in the prediction of toddlers' EBH: COVID-19-related social isolation was found to be a significant predictor of both emotional/behavioral competencies and problems, but only among toddlers exposed to higher levels of caregiving distress. This study evidences the negative impact of the COVID-19 crisis on the functioning of Portuguese families and toddlers' EBH. It emphasizes the importance for policies to consider the implications of the COVID-19 crisis for young children, and to provide psychosocial support to families in order to reduce caregiving distress and, thus, prevent children's mental health problems.

Psychometric Properties of the Parent-Report Version of the UCLA PTSD Reaction Index for DSM-5.

The UCLA PTSD Reaction Index for DSM-5 (RI-5) is a developmentally appropriate and well recognized screening tool for the assessment of Post-Traumatic Stress Disorder (PTSD) in children and adolescents. The children/adolescent self-report version of the RI-5 has been thoroughly studied in very recent years, showing adequate psychometric properties. Despite such results, the utility of the parent/caregiver-report version, which also integrates the RI-5 system, remains to be explored. As such, the present study aimed to test the general psychometric properties of the parent/caregiver-report version of RI-5, based on a sample of 457 children and adolescents, aged between 7 and 17 years, exposed to at least one potentially traumatic experience, and their respective primary caregivers. The RI-5 total score and categories revealed good internal consistency reliability. The total number of traumas reported emerged as a significant predictor of the RI-5 total score. The RI-5 total score proved to be significantly correlated with more internalizing and externalizing problems, but correlation coefficients were below .70, serving as an indicator of discriminant validity. The four-factor structure of the RI-5 was supported through confirmatory factor analysis. In conclusion, the present study provided preliminary evidence supporting the utility of the parent/caregiver-report version of the RI-5 for research purposes and for clinical assessment and treatment, anchored on a multiple informant perspective of child psychopathology.

Beliefs about sensitive parenting among mothers of children with disabilities.

The relevance of the quality of parent-child interactions for child development has long been established. Nevertheless, research on beliefs about maternal sensitivity is still scarce, to an even greater extent in the context of child disability. This inquiry aimed to describe beliefs about sensitive parenting among mothers of children with developmental disabilities and to examine how those beliefs relate to sociodemographic factors and perceived stress. Participants included 40 mothers with a child up to 7 years of age with a developmental disability. Mothers reported on sociodemographic factors and daily hassles and sorted an adapted version of the Maternal Behavior Q-Sort. Results revealed a strong convergence between maternal beliefs about the ideal mother and the attachment theory's concept of sensitivity. Daily hassles predicted beliefs about the ideal mother. These results underline the importance of implementing interventions in the context of child disability aimed at reducing parental stress.

Maternal and paternal overprotection of children born preterm: Relations to child and parental factors.

The purpose of this study was to investigate the correlates of observed overprotective behaviors of mothers and fathers of preterm-born preschoolers. Participants included 85 children born prematurely (gestational age <37 weeks) and their parents, assessed when children were 12, 24, and 42 months old. Observed overprotection was measured through the standardized observation of a parent-child interaction. The examined correlates included parent-reported perception of their protective behavior, parental gender, child neonatal risk, child development, parent-reported perception of child vulnerability, parent-reported psychological distress, and family's socioeconomic disadvantage. Results revealed that the correlation between parent-reported and observed parental overprotection was not significant for mothers and fathers. There was a significant positive association between maternal and paternal observed overprotection and no significant differences between mothers' and fathers' overprotection levels. Lower child developmental level and more socioeconomic disadvantage at child's 12 months of age were significant predictors of observed overprotective behaviors. Findings suggest the importance of targeting parents of preterm-born children with socioeconomic disadvantages and whose children have a lower developmental level for providing support to both mothers and fathers in the development of more adaptive parenting strategies. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

The Quality of Interactive Behaviors in the Context of Prematurity: Fathers, Mothers, Daughters, and Sons.

OBJECTIVE: This study examined the quality of interaction between preterm-born preschoolers and their mothers and fathers, focusing on the role of child and parental sex. METHODS: Participants included 88 preterm-born children (<37 wk gestational age) and 44 full-term-born children (≥37 wk gestational age) aged 3 1/2 years and their parents. Mother-child and father-child dyads were observed during a structured interactive task. Children's cooperation-compliance and negativity-hostility behaviors were coded using the Coding System for Mother-Child Interactions, and parents' sensitive behavior was coded using the Ainsworth Sensitivity Scale. RESULTS: There was no association between preterm birth and the quality of child and parents' interactive behaviors. In the full-term group, fathers exhibited lower levels of sensitive behavior than mothers, but in the preterm group, both parents exhibited similar levels of sensitive behavior. Preterm boys exhibited more interactive difficulties than preterm girls, but there was no significant effect of child sex on full-term children's interactive behaviors. Children exhibited more compliance-cooperation and less negativity-hostility toward fathers than toward mothers. CONCLUSION: The findings suggest that prematurity in itself does not affect the quality of parent-preterm child interactive behaviors as the children enter the preschool period. Preterm boys seem to be at higher risk for interactive difficulties than girls, and thus, child sex should be considered when monitoring and examining the development of preterm children.

Lifelong mercury bioaccumulation in Atlantic horse mackerel (Trachurus trachurus) and the potential risks to human consumption.

Atlantic horse mackerel is one of the most commercially important species in Europe. It can reach a longevity of 30 years, with potential implications in lifespan mercury contamination. This study conducted along the Portuguese coast aimed at evaluating the total Hg content and tissue distribution, to determine the annual mercury bioaccumulation patterns and the associated risk for consumption. The T-Hg accumulation patterns observed followed the order: muscle (0.34) > liver (0.28) > heart (0.19) > gills (0.11) > brain (0.041 mg kg-1). Significant differences between tissues reflect the role of the different tissues in storage and redistribution. Significant relationships observed between age and T-Hg for all tissues highlight the continuous nature of the bioaccumulation process. European food safety guidelines signalled significant risk of consumption in about 30% of the samples. Still, there was an overall low risk from the consumption of this species, which can be further minimized through consumer options to avoid health issues.

Function of estuaries and coastal areas as nursery grounds for marine fish early life stages.

The present study describes the larval and juvenile fish fauna of an estuary and its adjacent coastal area (Mondego estuary, Northwest coast of Portugal) and evaluates their function as nurseries for marine fish. For this, larvae and juveniles were sampled in both systems. The temporal and spatial patterns of the ichthyoplankton community were described for each system and related to the influence of environmental factors. Additionally, the recruitment pattern was evaluated based on the composition of juveniles. Results show a seasonal variation of larval density and community structure between and within systems, indicating a degree of segregation according to their ecological functional classification. Temperature was the most important environmental factor structuring the communities. The juvenile recruitment patterns observed show a different nursery function of the estuary and coastal area for early life stages of different species, reinforcing the need to integrate larval and juvenile stages to better understand fish life cycles and the connectivity between systems.

The functional composition of nearshore fish communities demonstrated by trait analysis: Response to environmental gradients.

There is limited understanding on nearshore fish community functioning in relation to changing environmental gradients. To address this, we investigated the trait composition of fish communities in five regions along the Portuguese coast during 4 years. Northern regions were characterized by higher Chlorophyll-α and small-sized species with short lifespans. The southernmost region was characterized by warmer waters, more intense upwelling events and typical warm-water species. Higher species richness and functional redundancy at the southernmost region suggested that in case of potential species loss, community functioning would be less affected in this region, and thus, a certain level of community resilience is maintained. The central region presented lower functional redundancy and high functional richness, which indicated the presence of rare species with rare traits, suggesting a more vulnerable community. This information on the nearshore fish community distribution may be particularly important towards managing coastal ecosystems in response to changing environmental conditions.

Mothers' distress exposure and children's withdrawn behavior - A moderating role for the Interferon Gamma gene (IFNG).

The dysregulation of the inflammatory response, including pro-inflammatory molecules, produces neuropsychiatric symptoms and depression-like behavior, including withdrawal from the physical and social environment. Genetic variants that enhance immune reactivity may thus increase inflammatory and withdrawn reactions to stress. Here we investigated a functional polymorphism of Interferon Gamma gene (IFNG +874 T > A, rs2430561) as moderator of the relationship between mothers' distress exposure and children's withdrawn behavior at preschool age. Participants were 198 Portuguese preschool children (mean age = 57.98 months). Exposure to mother's distress was assessed using the Brief Symptom Inventory, and withdrawn behavior with the Caregiver Teacher Report Form. All children provided saliva samples for genotyping. Contrary to expecations based on prior work, the rs2430561 AA genotype-not the T variant-interacted with (high levels of) mothers' distress exposure, to increase children's withdrawn behavior. No significant main effects were detected. The polymorphism in Interferon Gamma gene showed specific environmental stressor-dependent effects on withdrawn behavior during childhood, ones which are interpreted in light of the "behavioral immune system" hypothesis, and which proved inconsistent with diathesis-stress thinking.

Mercury accumulation in fish species along the Portuguese coast: Are there potential risks to human health?

This paper aimed at evaluating the total mercury content in five common fish species from the western European Atlantic coastal waters, and the associated risk of consumption. Mercury concentrations in muscle of Atlantic mackerel (Scomber scombrus), Atlantic chub mackerel (Scomber colias), European anchovy (Engraulis encrasicolus), Atlantic horse mackerel (Trachurus trachurus) and European pilchard (Sardina pilchardus) ranged from 0.003 to 0.20 mg kg-1 wet weight, and no significant differences were observed between the average concentration in each species. A significant increasing trend in mercury content with fish size was observed for all species (except for European anchovy), suggesting mercury bioaccumulation throughout their life cycle. Still, the mercury content was far below the European Food Safety Authority and World Health Organization food safety thresholds in all species, highlighting the low risk to human health due to the ingestion of these species and the importance of consumer options for risk reduction.

Early family adversity, stability and consistency of institutional care and infant cognitive, language and motor development across the first six months of institutionalization.

This study extends research on the effects of institutionalization-by examining the trajectories of cognitive, language and motor development of 64 Portuguese infants and toddlers across the first six months of institutionalization, while determining whether pre-institutional adversities and the stability and consistency of institutional care predict children's development. At time of enrollment, 23.4%, 32.8% and 31.3% of the children were moderately to severely delayed, respectively, in their cognitive, linguistic and motor functioning. Developmental problems persisted after six months of institutionalization. The accumulation of early pre-institutional adversities predicted cognitive and motor limitations at admission to the institutions, but not variation in subsequent development. The stability and consistency of institutional care also failed to predict developmental growth and change. Children who had never lived with their families of origin showed a better language development at enrollment than their counterparts who had lived with their families of origin before institutionalization. Such advantage was followed by a deceleration in language growth after six months of institutional placement. Results are discussed in terms of short- vs. longer-term effects of institutionalization.

Maternal Interactive Behaviours in Parenting Children with Williams Syndrome and Autism Spectrum Disorder: Relations with Emotional/Behavioural Problems.

This study compared maternal responsiveness to children with two neurodevelopmental disorders sharing different but, in some cases, overlapping social phenotypes-Williams syndrome (WS) and autism spectrum disorder (ASD)-and explored the relations between maternal responsiveness and child emotional/behavioural problems (EBP). The sample included 16 pre-schoolers with WS and 43 with ASD, and their mothers. Responsiveness was assessed during a mother-child interaction task. Mothers completed the CBCL 1½-5, providing a measure of EBP. No significant differences emerged between groups, and most dyads were characterized by less responsive behaviours. Maternal responsiveness proved related to child developmental age, but not with EBP. These results provide further insight into the rearing environment of children with neurodevelopmental disorders, highlighting the need for early relationship-based interventions.

Development and validation of an observational measure of symptoms of Reactive Attachment Disorder.

Reactive Attachment Disorder (RAD) is presumed to be a consequence of social neglect and deprivation of the kind particularly associated with institutional care. Despite its clinical relevance there is a lack of assessment tools for RAD based on the direct observation of child-caregiver interaction. Here we describe the development and validation of such a tool for use with preschool children, the Rating of Inhibited Attachment Disordered Behavior (RInAB). The RInAB is composed of 17 ratings grouped in three subscales assessing (1) Attachment, (2) Exploratory, and (3) Socioemotional behavior. Participants were 134 institutionalized preschool children (M = 54.84 months; SD = 10.83; 60% boys) and their caregivers. Adequate reliability was found for RInAB subscales and total score. Confirmatory factor analyses documented the three aforementioned RInAB subscales. Correlational analyses documented: (i) construct validity via positive and significant associations with caregiver sensitivity and quality of child-caregiver relationship; (ii) convergence validity via association evidence with some emotionally/withdrawn inhibited items of the Disturbed Attachment Interview (DAI), as well as, with Child Behavior Checklist (CBCL)'s somatic complaints and withdraw syndrome scales; and (iii) discriminant validity via nonsignificant or negative associations with DAI-indiscriminate subscale, Rating of Infant and Stranger Engagement (RISE) and CBCL-externalizing problems. Discussion highlights the contributions complementary roles of RInAB for a comprehensive assessment of child RAD-related functioning.

Child's oxytocin response to mother-child interaction: The contribution of child genetics and maternal behavior.

The oxytocinergic system is a primary biological system involved in regulating a child's needs for bonding and for protection from threats. It is responsive to social experiences in close relationships, though evidence across studies is not entirely consistent. Guided by previous literature, we investigated individual and environmental factors predicting and presumably affecting children's oxytocin (OT) response during mother-child interaction. by focusing on children's OXTR genotype, and maternal behavior, respectively. This was achieved by assessing salivary OT levels of 88 Portuguese preschoolers prior to and following a mother-child interaction task, and by genotyping children's OXTR SNP rs53576. Maternal interactive behavior was assessed using Ainsworth scales. Results indicated that child genotype and mother's sensitive responsiveness interacted in predicting change in child OT concentrations from before to after the interaction. Specifically, Genotypic differences emerged under conditions of low maternal sensitive responsiveness: OT levels increased over time for children with the GG genotype when maternal sensitive responsiveness was low, but no such genotypic differences were evident when mothers were highly sensitive responsive. Findings provide preliminary support for the notion that increased understanding of children's OT and close relationships requires consideration of both individual and environmental factors.

EARLY MALTREATMENT AND CURRENT QUALITY OF RELATIONAL CARE PREDICT SOCIOEMOTIONAL PROBLEMS AMONG INSTITUTIONALIZED INFANTS AND TODDLERS.

The present study is focused on child socioemotional problems 6 months after institutionalization, by considering the putative predictive role of child maltreatment, of developmental functioning at admission and the following months, and of the quality of institutional relational care. Fifty institutionalized infants and toddlers participated in this study. Child developmental functioning (i.e., cognitive, language, and motor development) was assessed at admission to the institution (Wave 0), and 3 (Wave 1) and 6 months (Wave 2) thereafter. The quality of institutional relational care-operationalized in terms of caregivers' sensitivity and cooperation-was measured at Wave 2. Caregivers reported on the presence of disturbed socioemotional behaviors at Wave 2. Child gestational age, birth weight, age, and stunted growth at admission to the institution served as covariates. Results revealed significant associations between socioemotional difficulties and lower levels of motor development at Waves 0 and 1, child maltreatment, and less sensitive caregiving. A logistic regression showed that child maltreatment and caregiver insensitivity were the only significant predictors of disturbed socioemotional functioning by the end of 6 months of institutionalization.

Being a mother of preterm multiples in the context of socioeconomic disadvantage: perceived stress and psychological symptoms / Ser mãe de múltiplos prematuros no contexto de desvantagem socioeconômica: estresse percebido e sintomas psicológicos

Abstract Objective: This study aimed to examine the differences between mothers of preterm multiples and mothers of preterm singletons regarding perceived stress and maternal psychological symptoms, and to explore the putative adverse amplified effect of socioeconomic disadvantage. Method: Ninety-five mothers of 1-year-olds born preterm participated in this cross-sectional study. Data collection was carried out in two public hospitals from Northern Portugal. To assess maternal perceived daily stress and psychological symptoms, mothers completed two questionnaires. Mothers reported on socioeconomic factors, including family poverty, parent unemployment, and low education, and two groups of family socioeconomic disadvantage were created. A child medical risk index was calculated. Results: Results indicated that mothers of preterm multiples reported higher levels of stress than mothers of preterm singletons. Moreover, and specifically regarding psychological functioning, mothers of preterm multiples reported more symptoms than mothers of preterm singletons, but only when living in a context of socioeconomic adversity. Conclusions: The results of the present study have important implications for practice. Mothers of preterm multiples are at higher risk to present mental health difficulties, in comparison to mothers of singletons, especially when exposed to socioeconomic adversities. The development of psychosocial intervention programs and public policies are of decisive importance in helping mothers of multiples adjust to parenthood.

Resumo Objetivo: Examinar as diferenças entre mães de múltiplos prematuros e mães de filhos únicos prematuros a respeito de estresse percebido e sintomas psicológicos maternos e explorar o efeito adverso amplificado putativo da desvantagem socioeconômica. Método: Participaram deste estudo transversal 95 mães de crianças de um ano nascidas prematuras. A coleta de dados foi feita em dois hospitais públicos do norte de Portugal. Para avaliar o estresse diário percebido e os sintomas psicológicos maternos, as mães responderam dois questionários. As mães relataram fatores socioeconômicos, incluindo pobreza familiar, desemprego dos pais e baixo nível de escolaridade, e foram criados dois grupos de desvantagem socioeconômica familiar. Foi calculado um índice de risco médico infantil. Resultados: Os resultados indicaram que as mães de múltiplos prematuros relataram maiores níveis de estresse do que as mães de filhos únicos prematuros. Além disso e especificamente com relação ao funcionamento psicológico, as mães de múltiplos prematuros relataram mais sintomas do que as mães de filhos únicos prematuros, porém apenas quando moravam em um contexto de adversidade socioeconômica. Conclusões: Os resultados deste estudo possuem importantes implicações para a prática. As mães de múltiplos prematuros apresentam maior risco de dificuldades de saúde mental, em comparação a mães de filhos únicos, principalmente quando expostas a adversidades socioeconômicas. O desenvolvimento de programas de intervenção psicossocial e políticas públicas é de importância decisiva ao ajudar as mães de filhos múltiplos a se ajustarem à maternidade.

The Di-iron RIC Protein (YtfE) of Escherichia coli Interacts with the DNA-Binding Protein from Starved Cells (Dps) To Diminish RIC Protein-Mediated Redox Stress.

The RIC (repair of iron clusters) protein of Escherichia coli is a di-iron hemerythrin-like protein that has a proposed function in repairing stress-damaged iron-sulfur clusters. In this work, we performed a bacterial two-hybrid screening to search for RIC-protein interaction partners in E. coli As a result, the DNA-binding protein from starved cells (Dps) was identified, and its potential interaction with RIC was tested by bacterial adenylate cyclase-based two-hybrid (BACTH) system, bimolecular fluorescence complementation, and pulldown assays. Using the activity of two Fe-S-containing enzymes as indicators of cellular Fe-S cluster damage, we observed that strains with single deletions of ric or dps have significantly lower aconitase and fumarase activities. In contrast, the ric dps double mutant strain displayed no loss of aconitase and fumarase activity with respect to that of the wild type. Additionally, while complementation of the ric dps double mutant with ric led to a severe loss of aconitase activity, this effect was no longer observed when a gene encoding a di-iron site variant of the RIC protein was employed. The dps mutant exhibited a large increase in reactive oxygen species (ROS) levels, but this increase was eliminated when ric was also inactivated. Absence of other iron storage proteins, or of peroxidase and catalases, had no impact on RIC-mediated redox stress induction. Hence, we show that RIC interacts with Dps in a manner that serves to protect E. coli from RIC protein-induced ROS.IMPORTANCE The mammalian immune system produces reactive oxygen and nitrogen species that kill bacterial pathogens by damaging key cellular components, such as lipids, DNA, and proteins. However, bacteria possess detoxifying and repair systems that mitigate these deleterious effects. The Escherichia coli RIC (repair of iron clusters) protein is a di-iron hemerythrin-like protein that repairs stress-damaged iron-sulfur clusters. E. coli Dps is an iron storage protein of the ferritin superfamily with DNA-binding capacity that protects cells from oxidative stress. This work shows that the E. coli RIC and Dps proteins interact in a fashion that counters RIC protein-induced reactive oxygen species (ROS). Altogether, we provide evidence for the formation of a new bacterial protein complex and reveal a novel contribution for Dps in bacterial redox stress protection.