RESUMO
The activity of important glycolytic enzymes (hexokinase, phosphofructokinase, aldolase, phosphohexoseisomerase, pyruvate kinase and lactate dehydrogenase) and glutaminolytic enzymes (phosphate-dependent glutaminase) was determined in the thymus and mesenteric lymph nodes of Wistar rats submitted to protein malnutrition (6% protein in the diet rather than 20%) from conception to 12 weeks after birth. The wet weight (g) of the thymus and mesenteric lymph nodes decreased due to protein malnutrition by 87% (from 0.30 +/- 0.05 to 0.04 +/- 0.01) and 75% (0.40 +/- 0.04 to 0.10 +/- 0.02), respectively. The protein content was reduced only in the thymus from 102.3 +/- 4.4 (control rats) to 72.6 +/- 6.6 (malnourished rats). The glycolytic enzymes were not affected by protein malnutrition, but the glutaminase activity of the thymus and lymph nodes was reduced by half in protein-malnourished rats as compared to controls. This fact may lead to a decrease in the cellularity of the organ and thus in its size, weight and protein content.
Assuntos
Glucose/metabolismo , Glutamina/metabolismo , Glicólise/fisiologia , Linfonodos/enzimologia , Desnutrição Proteico-Calórica/enzimologia , Timo/enzimologia , Animais , Proteínas Alimentares/metabolismo , Frutose-Bifosfato Aldolase/metabolismo , Glucose-6-Fosfato Isomerase/metabolismo , Glutaminase/metabolismo , Hexoquinase/metabolismo , L-Lactato Desidrogenase/metabolismo , Masculino , Fosfofrutoquinase-1/metabolismo , Piruvato Quinase/metabolismo , Ratos , Ratos WistarRESUMO
The activity of important glycolytic enzymes (hexokinase, phosphofructokinase, aldolase, phosphohexoseisomerase, pyruvate kinase and lactate dehydrogenase) and glutaminolytic enzymes (phosphate-dependent glutaminase) was determined in the thymus and mesenteric lymph nodes of wistar rats submited to protein malnutrition (6 percent protein in the diet rather than 20 percent) from conception to 12 weeks after birth. The wet weight (g) of the thymus and mesenteric lymph nodes decreased due to protein malnutrition by 87 percent (from 0.30 + 0.05 to 0.04 + 0.01) and 75 percent (0.40 + 0.04 to 0.10 + 0.02), respectively. The protein content was reduced only in the thymus from 102.3 + 4.4 (control rats) to 72.6 + 6.6 (malnourished rats). The glycolytic enzymes were not affected by protein malnutrition, but the glutaminase activity of the thymus and lymph nodes was reduced by halfing in protein-malnourished rats as compared to controls. This fact may lead to a decrease in the cellularity of the organ and thus in its size, weight and protein content.
Assuntos
Ratos , Masculino , Animais , Proteínas Alimentares/metabolismo , Frutose-Bifosfato Aldolase/metabolismo , Glucose-6-Fosfato Isomerase/metabolismo , Glucose/metabolismo , Glutaminase/metabolismo , Glutamina/metabolismo , Glicólise/fisiologia , Hexoquinase/metabolismo , L-Lactato Desidrogenase/metabolismo , Linfonodos/enzimologia , Fosfofrutoquinase-1/metabolismo , /metabolismo , Desnutrição Proteico-Calórica/enzimologia , Piruvato Quinase/metabolismo , Timo/enzimologia , Ratos WistarRESUMO
Large unilamellar vesicles, prepared with dioctadecyldimethylammonium chloride, entrap nitroblue tetrazolium. Addition of solid KO2, or production of superoxide anion by riboflavin photolysis, to nitroblue tetrazolium-containing dioctadecyldimethylammonium vesicles results in the formation of monoformazan above the phase-transition temperature of the bilayer. Below the phase-transition temperature the yield of monoformazan is negligible. These results demonstrate that superoxide anion permeates vesicles above the phase-transition temperature of the bilayer.
Assuntos
Bicamadas Lipídicas/química , Superóxidos/química , Ânions/química , Micelas , Fotólise , Compostos de Amônio Quaternário , TemperaturaRESUMO
Em 109 escolares de 8 a 12 anos de idade provenientes de zona periferica da cidade de Campinas-SP,encontrou-se uma prevalencia de parasitos intestinais de 42,2%, a media de concentraçao da hemoglobina sanguinea foi de 13,57g/dl 0,98 e concentraçao serica de ferritina de 43,67 ng/ml 21,34. No estudocomparativo entre a populaçao infectada e nao infectada por parasitas intestinais nao houve diferenças estaristicamente significativas para dosagem de hemoglobina e ferritina serica.