Biosolid as an alternative source of nutrients in chrysanthemum cultivation.

The objective was to evaluate the biosolids as an alternative source of nutrients in the production of chrysanthemums by adding increasing doses to the cultivation substrate. The experimental design was in blocks with 6 treatments and 5 replications. The treatments consisted of the mixture (commercial substrate + biosolid) at the concentrations: 20%, 40%, 60% and 80% of biosolid + two controls (100% of biosolid and 100% of substrate). The experiment was conducted in a greenhouse for 90 days. Physiological parameters, number of flower buds, dry biomass and nutrient accumulation were evaluated. Physiological parameters were evaluated using the Infrared Gas Analyzer. The number of flower buds was evaluated by counting. Biomass was determined after drying the structures and then calculated the accumulation of nutrients. A total of 90 plants were evaluated. Concentrations of up to 40% of biosolid promoted a greater number of flower buds, dry biomass and nutrient accumulation. Concentrations above 60% lower number of buds, biomass increment and nutrient accumulation. It is concluded that the biosolid has potential as an alternative source of nutrients in the cultivation of chrysanthemums, indicating concentrations of up to 40% and the nutrient content of each batch generated must be verified.

Interface entre valorização, reconhecimento e satisfação do trabalho de enfermeiros da Atenção Primária à Saúde / Interface between valorization, recognition, and satisfaction of nurses' work in Primary Health Care / Interfaz entre valorización, reconocimiento y satisfacción del trabajo de enfermeras en Atención Primaria de Salud

Objetivo:identificar a interface entre valorização, reconhecimento e satisfação advindas das percepções de enfermeiros no desenvolvimento do trabalho na Atenção Primária à Saúde. Método:estudo qualitativo, descritivo e exploratório, realizado com 132 enfermeiros de 23 municípios de Minas Gerais. Os dados foram coletados por meio de formulário on-line e analisados conforme pressupostos da análise de conteúdo, com amparo do software IraMuteQ.Resultados: a maioria dos participantes eram do sexo feminino, idade média de 36 anos, carga horária semanal de 40 horas e recebiam entre dois e quatro salários-mínimos. Os dados foram organizados em uma categoria denominada: Interface entre a valorização, o reconhecimento e a satisfação, advindas das percepções de enfermeiros no desenvolvimento do trabalho na atenção primária em saúde. Conclusões:a interface entre os fenômenos evidenciou os que permeiam o salário, a carga horária, o reconhecimento de terceiros, o valor social, a importância da profissão e a autonomia.

Objective:to identify the interfaces between appreciation, recognition, and satisfaction arising from the perceptions of nurses in the development of work in Primary Health Care. Method: this qualitative, descriptive, and exploratory study was conducted with 132 nurses from 23 municipalities in Minas Gerais. Data were collected using an online form and analyzed according to content analysis assumptions, supported by the IraMuteQsoftware. Results:the majority of participants were female, with an average age of 36 years, worked 40 hours per week, and received between two and four minimum wages. The data were organized into a category called: Interfaces between appreciation, recognition, and satisfaction arising from the perceptions of nurses in the development of work in primary health care. Conclusions: the interface between the phenomena showed that points of convergence permeate salary, working hours, recognition from third parties, social value, the importance of the profession, and autonomy.

Objetivo: identificar interfaces entre apreciación, reconocimiento y satisfacción surgidos de percepciones de enfermeras en el desarrollo del trabajo en Atención Primaria de Salud. Método: estudio cualitativo que se realizó con 132 enfermeras de 23 municipios en Minas Gerais. Los datos se recopilaron mediante formulario en línea y por análisis de contenido, con el apoyo del software IraMuteQ. Resultados: La mayoría de los participantes eran mujeres, con una edad promedio de 36 años, trabajaban 40 horas por semana y recibían entre dos y cuatro salarios mínimos. Los datos se organizaron en una categoría denominada: Interfaces entre apreciación, reconocimiento y satisfacción surgidos de las percepciones de las enfermeras en el desarrollo del trabajo en atención primaria de salud. Conclusiones: La interfaz entre los fenómenos mostró que los puntos de convergencia permeaban el salario, las horas de trabajo, elreconocimiento de terceros, el valor social, la importancia de la profesión y la autonomía.

Purification and use of crude green glycerol from the transesterification of triglycerides in the formulation of an alcohol gel hand sanitizer.

The aim of this study was to produce an alcohol gel hand sanitizer containing green glycerol. Crude glycerol was purified using chemical and physical treatments. The sanitizer was prepared using 71.100 g of 99.3° GL ethanol, 28.0 g H2O, 0.5 g of Carboxypolymethylene [Carbopol 940® or Carbomer], 5 drops of triethanolamine (pH 5-7), and glycerol (1.5% w/w). The thermal behavior of the ethanol, carbopol, triethanolamine, glycerol, and alcohol gels were evaluated using Thermogravimetry and Differential Thermal Analysis. The apparent viscosity was obtained using a rotary viscometer. The determination of in vitro spreadability was achieved by an adaptation of the Knorst method. The ethanol content was measured by headspace gas chromatography using a flame ionization detector. The thermal behavior of the gels was influenced by the presence of glycerol, which confirms the possible network interactions formed. The relative densities of the samples were between 0.887 and 0.890 g/cm3. No alteration of the pH of the formulation resulted from the incorporation of glycerol. The apparent viscosities of the alcohol gels were greater than 20,000 cP. No alteration in the in vitro spreadability of the gel alcohol (530.6 mm2) resulted from the addition of glycerol. Hand sanitizer was produced using glycerol from a transesterification reaction. It represents an alternative use for the glycerol being produced in biodiesel processes. The product satisfied the requirements of WHO that preconize a formulation containing 1.45% glycerol as an humectant to protect skin against dryness and dermatitis.

Toxicity of Heavy Metals that Affect Germination, Development and Cell Cycle of Allium cepa L.

This study aimed to investigate the impact of heavy metals copper, cadmium, lead, aluminum and nickel, on the growth, physiology, metabolism, and cell cycle of Allium cepa L. Five treatments with increasing concentrations (0, 50, 100, 250, and 500 µM) were applied to the seeds. The results showed that the highest concentrations of copper and cadmium had phytotoxic and biochemical effects on the onion. Additionally, copper concentrations caused an increase in mitodepressive effect and chromosomal abnormalities. Aluminum also induced several chromosomal abnormalities. The study found that Cd > Cu > Pb > Ni > Al and Cu > Al > Ni > Pb > Cd had the highest phytotoxic and cytotoxic potentials, respectively. Furthermore, the UPGMA method revealed three divergent groups. These results suggest that heavy metals, especially copper, have a significant pollution potential when present in high concentrations.

Environmentally Friendly New Catalyst Using Waste Alkaline Solution from Aluminum Production for the Synthesis of Biodiesel in Aqueous Medium.

Red mud (RM) is composed of a waste alkaline solution (pH = 13.3) obtained from the production of alumina. It contains high concentrations of hematite (Fe2O3), goethite (FeOOH), gibbsite [Al(OH)3], a boehmite (AlOOH), anatase (Tetragonal-TiO2), rutile (Ditetragonal dipyramidal-TiO2), hydrogarnets [Ca3Al2(SiO4)3-x(OH)4x], quartz (SiO2), and perovskite (CaTiO3). It was shown to be an excellent catalytic mixture for biodiesel production. To demonstrate the value of RM, an environmentally friendly process of transesterification in aqueous medium using waste cooking oil (WCO), MeOH, and waste alkaline solution (WAS) obtained from aluminum production was proposed. Triglycerides of WCO reacted with MeOH at 60 °C to yield mixtures of fatty acid methyl esters (FAMEs) in the presence of 0.019% (w/w) WAS/WCO using the WAS (0.204 mol L-1, predetermined by potentiometric titration) from aluminum production by the Bayer process. The use of the new catalyst (WAS) resulted in a high yield of the products (greater than 99% yield).

Tandem Transesterification-Esterification Reactions Using a Hydrophilic Sulfonated Silica Catalyst for the Synthesis of Wintergreen Oil from Acetylsalicylic Acid Promoted by Microwave Irradiation.

SiO2-SO3H, with a surface area of 115 m2/g and pore volume of 0.38 cm3g-1, and 1.32 mmol H+/g was used as a 20% w/w catalyst for the preparation of methyl salicylate (wintergreen oil or MS) from acetylsalicylic acid (ASA). A 94% conversion was achieved in a microwave reactor over 40 min at 120 °C in MeOH. The resulting crude product was purified by flash chromatography. The catalyst could be reused three times.

Catalytic Transformation of Triglycerides to Biodiesel with SiO2-SO3H and Quaternary Ammonium Salts in Toluene or DMSO.

SiO2-SO3H, with a surface area of 115 m2·g-1, pore volumes of 0.38 cm3·g-1 and 1.32 mmol H+/g, was used as a transesterification catalyst. Triglycerides of waste cooking oil reacted with methanol in refluxing toluene to yield mixtures of diglycerides, monoglycerides and fatty acid methyl esters (FAMEs) in the presence of 20% (w/w) catalyst/oil using the hydrophilic sulfonated silica (SiO2-SO3H) catalyst alone or with the addition of 10% (w/w) co-catalyst/oil [(Bun4N)(BF4) or Aliquat 336]. The addition of the ammonium salts to the catalyst lead to a decrease in the amounts of diglycerides in the products, but the concentrations of monoglycerides increased. Mixtures of (Bun4N)(BF4)/catalyst were superior to catalyst alone or Aliquat 336/catalyst for promoting the production of mixtures with high concentrations of FAMEs. The same experiments were repeated using DMSO as the solvent. The use of the more polar solvent resulted in excellent conversion of the triglycerides to FAME esters with all three-catalyst media. A simplified mechanism is presented to account for the experimental results.

Cadmium-induced transgenerational effects on tomato plants: A gift from parents to progenies.

The present study aimed to investigate the Cd-induced transgenerational effects on plants. Grafted tomato plants, which exhibited the same cultivar as scion and distinct cultivars with contrasting Cd-tolerance as rootstocks, were grown in soil without and with artificial addition of Cd (less than 2.0, and 6.9 mg kg-1 of Cd, respectively) in a pot experiment carried out in a greenhouse. Their fruits were harvested to extract seeds (i.e., the progenies), which were sown over either Cd-free (control) or Cd-containing germitest paper (germination testing paper with 0 and 35 µM of CdCl2, respectively) and grown in a growth chamber. The immediate progeny of all grafting combinations from stressed plants presented an elevated germinability, despite high internal Cd concentration. When sown in Cd-containing germitest paper, the immediate progeny of plants grown in soil with no Cd addition was generally able to maintain or even increase the content of carotenoids and chlorophylls a and b (up to 93.3, 62.8 and 76.1%, respectively), indicating a Cd-induced hormetic effect on photosynthetic pigments. Two of the grafting combinations from stressed plants yielded seeds that generated seedlings with enhanced dry mass when they were sown in Cd-free media (~41%), suggesting a Cd-induced transgenerational enhancement of biomass production. Because only one tomato cultivar was used as scion, data indicated that type and degree of Cd-induced transgenerational effects depend strongly on signals generated and/or processed in roots of the parental plants. When sown in Cd-contaminated germitest paper, the immediate progeny of Cd-treated plants presented major reductions in the leaf area (35-69%) and content of photosynthetic pigments (57-93%) in comparison to the progeny from control plants. However, one of the grafting combinations exhibited satisfactory performance after "double" exposure to Cd, showing 91% of the biomass that was produced in the seedlings of control seeds from control plants. Further investigation indicated that adjustments in the chlorophyll fluorescence behavior might counterbalance losses in leaf pigments and area. Taken together, our data provide new insights on the origin, outcomes and mode of action of the Cd-induced transgenerational effects.

Dehydration of D-fructose to 5-hydroxymethyl-2-furfural in DMSO using a hydrophilic sulfonated silica catalyst in a process promoted by microwave irradiation.

SiO2-SO3H, with a surface area of 115 m2/g, pore volumes of 0.38 cm3g-1 and 1.32 mmol H+/g, was used as a 10% w/w catalyst for the preparation of 5-hydroxymethyl-2-furfural (HMF) from fructose. A conversion of 100% was achieved in a microwave reactor during 10 min at 150 °C in DMSO, with 100% selectivity for HMF, at a molar ratio of fructose: DMSO equal to 1:56. The catalyst could be re-used three times.

Allelochemical Activity of Eugenol-Derived Coumarins on Lactuca sativa L.

Coumarins are widely distributed substances in plant species that promote phytotoxic effects, allowing them to be exploited as herbicides less harmful to the environment, since many invasive species have demonstrated resistance to commercially available products. The derived coumarins used in this study had not been tested in plant models and their effect on plants was unknown. The objective of this study was to evaluate the phytotoxic action of these coumarins in bioassays with Lactuca sativa L., in order to select the most responsive substance whose toxicity was best elucidated by chromosomal complement and enzymatic antioxidant metabolism studies. From the phytotoxicity assays, coumarin 8-methoxy-2-oxo-6-(prop-2-en-1-yl)-2H-chromene-3-carboxylic acid (A1), reported here for the first time, was selected as the most responsive and caused a reduction in the following parameters: number of normal seedlings, fresh biomass, root length and shoot length. Subsequent studies demonstrated that this coumarin is cytogenotoxic due to damage caused to the cell cycle and the occurrence of chromosomal abnormalities. However, it did not interfere with antioxidant enzyme activity and did not cause lipid peroxidation. The changes caused by coumarin A1 described herein can contribute to better understanding the allelochemical actions of coumarins and the potential use of these substances in the production of natural herbicides.

Eficácia de uma nova geração de xampus no controle da dermatite seborreica do couro cabeludo / Efficacy of a new generation of shampoos in controlling seborrheic dermatitis of the scalp

Introdução: A dermatite seborreica é uma doença inflamatória crônica sobre áreas com maior concentração de glândulas sebáceas, com participação de fungos do gênero Malassezia sp. Objetivo: Avaliar a eficácia do tratamento da dermatite seborreica moderada a intensa com o uso de duas formulações de xampu em monoterapia. Métodos: Pacientes portadores de dermatite seborreica de couro cabeludo moderada à intensa, com cabelos processados quimicamente ou não, foram agrupados de acordo com o grau da afecção e usaram uma das duas versões de xampus em monoterapia, por quatro semanas. Também foi avaliada a ocorrência de recidivas após a suspensão do tratamento. Resultados: Houve redução significativa (p<0,05) da oleosidade desde a primeira aplicação. Os demais sinais (eritema e descamação) apresentaram melhora significativa para ambos os tratamentos. Também foi relatada uma melhora significativa do prurido, eritema e descamação pelos participantes. O efeito sobre os fios foi considerado positivo com ambos os tratamentos. Após uma semana da suspensão, o índice de recidivas foi considerado não significativo (p<0,05). Conclusões: As duas versões de xampus foram capazes de promover um controle efetivo da dermatite seborreica moderada à intensa. Essas formulações demonstraram também não agredirem os fios, mesmo quando processados, fato considerado fundamental para a adesão ao tratamento.

Introduction: Seborrheic dermatitis is a chronic inflammatory disease in areas with a higher concentration of sebaceous glands and the participation of the fungi of the genus Malassezia sp. Objective: To assess the effectiveness of treating moderate to severe seborrheic dermatitis using two shampoo formulations in monotherapy. Methods: Patients with moderate to severe seborrheic dermatitis of the scalp, with or without chemically processed hair, were grouped according to the degree of affection and used one of two versions of shampoo monotherapy for four weeks. Relapse after treatment cessation was also evaluated. Results: There was a significant reduction (p <0.05) of oiliness since the first application. The other signs (erythema and peeling) showed significant improvement for both treatments. Participants also reported a considerable improvement in pruritus, erythema, and peeling. The effect on the hair strands was considered positive with both procedures. After one week of suspension, the relapse rate was considered non-significant (p <0.05). Conclusions: Both versions of shampoos were able to promote effective control of moderate to severe seborrheic dermatitis. These formulations also demonstrated not to harm the hair strands, even when chemically processed, a fundamental fact to treatment adherence.

Synthesis of Phenyl Esters Using SiO2-SO3H Catalyst in Conventional Heating and Microwave-Irradiated Esterification Processes.

A SiO2-SO3H amorphous catalyst containing a small surface area of 115.0 m²g-1 and 1.32 mmol H+/g was prepared from fine construction sand and sodium carbonate and sulfonated with H2SO4. In a 10% (w/w) basis, it is very efficient for catalyzing the esterification of carboxylic acids with phenol. The reaction processes were performed using conventional heating and under microwave irradiation. The yields were higher in the microwave-irradiated esterification. The catalyst could be used for three esterification sequences in both processes.

Effect of Salicylic Acid, 2,4-D and 2i-P on the Production of Secondary Metabolites in Garcinia brasiliensis Mart. Callus

Abstract The aim of this work was to evaluate the effect of Salicylic acid, 2,4-D and 2-iP on the production of total phenolic and flavonoids as well in the levels of fukugetin and 7-epiclusianone in callus of Garcinia brasiliensis zygotic embryos. For this, Bacupari callus were exposed to different concentrations (0.0; 0.1; 1; 10; 100 µM) of Salicylic acid (SA) in the presence or in the absence of 72 μM 2-iP and 28.73 μM 2,4-D. The highest concentration of total phenolic occurred in the treatment with 100 μM SA in callus subcultured in the absence of 2,4-D and 2-iP, and with 10 μM SA in callus subcultured in the presence of these regulators. Concerning flavonoids, 2,4-D and 2-iP supplementation without the presence of the SA was sufficient for the highest levels. Additionally, it was possible to identify the fukugetin and 7-epiclusianone. However, the variation in the levels was very high, especially for the fukugetin. Therefore, in most treatments, there was no statistically difference. Except for the treatment with 10 μM SA + 2,4-D and 2-iP, where there was a significant increase in 7-epiclusianone.

The preparation of benzyl esters using stoichiometric niobium (V) chloride versus niobium grafted SiO2 catalyst: A comparison study.

Two solvent free methods of a one-to-one alcohol/acid mol ratio synthesis of benzyl esters of the formic, acetic, benzoic, salicylic, nicotinic, and oxalic acids are described. The stoichiometric reactions used 1.5 mol ratio solid NbCl5 as the reagent and required from two to three hours for completion at room temperature; for the catalytic processes, NbCl5 was grafted directly, at room temperature, onto a silica gel of specific area of 507 m2g-1, produced from construction sand and sodium carbonate, forming a 5.4% Nb w/w SiO2-Nb gel with a specific area of 412 m2g-1. At 10% w/w catalyst/alcohol ratio, this SiO2-Nb catalyst gave similarly very good yields but required from 6 to 9 hours at the reflux temperature of the slurry. The catalyst could be re-used three times.

Vitamin C and total phenols quantification in calli of native passion fruit induced by combinations of Picloram and Kinetin / Quantificação de vitamina C e fenóis totais em calos de maracujazeiro nativo induzidos por combinações de Picloram e Cinetina

Brazil is one of the center of origin of passion fruit and has an important natural variability of the genus Passiflora. Several wild species of this genus are resistant to some pests and diseases and many are considered as medicinal. The aim of this research was to induce callus from in vitro Passiflora gibertii leaf explants for quantification of vitamin C and total phenols. Once the appropriate auxin/cytokine balance promotes callus formation and may optimize the production of secondary compounds and vitamins, calli were induced using a half-strength MS medium with a combination of the auxin Picloran (PIC) and the cytokine Kinetin (KIN). The vitamin C and total phenols were quantified by colorimetric methods from calli after different culture periods. The calli induction was strongly dependent of the combination PIC/KIN. It was observed high vitamin C content (94.8mg 100g-1) during the calli induction period in MS+4.14µM PIC+ 0.207µM KIN. Higher PIC/KIN concentrations promoted an increase in the vitamin C content after three subcultures. The higher PIC (8.28µM)/KIN (0.828µM) concentration was the higher was the total phenols production (66mg tannic acid 100g-1 of fresh callus) during the calli induction period.

O Brasil é um dos centros de origem do maracujazeiro e possui uma importante variabilidade natural do gênero Passiflora. Muitas espécies selvagens desse gênero são resistentes a algumas pragas e doenças e várias são consideradas medicinais. O objetivo deste trabalho foi induzir calos in vitro a partir de folhas de Passiflora gibertii para a quantificação de vitamina C e fenóis totais. Uma vez que o balanço adequado entre auxina/citocinina promove a formação de calos e pode otimizar a produção de compostos secundários e vitaminas, calos foram induzidos em meio MS meia-força com uma combinação da auxina Picloram e da citocinina Cinetina, todos na ausência de luz. O teor de vitamina C e de fenóis totais foi quantificado por métodos colorimétricos após diferentes períodos de cultivo dos calos. A indução de calos foi fortemente influenciada pela combinação de Picloram/Cinetina. Foi observado um alto teor de vitamina C (94,8mg 100g-1) durante o período de indução de calos em MS+4,14µM de Picloram+0,207µM de Cinetina. Altas concentrações de Picloram/Cinetina conduzem a um aumento no teor de vitamina C após três subcultivos. Quanto maior a concentração de Picloram (8,28µM)/Cinetina (0,828µM), maior é a produção de fenóis (66mg de ácido tânico 100g-1 de calos) totais durante o período de indução de calos.

Decontaminant solution on in vitro growth of Byrsonima intermedia seedlings / Solução desinfestante no crescimento in vitro de plântulas de Byrsonima intermedia

Byrsonima intermedia A. Juss. é uma planta medicinal e frutífera do Cerrado, cuja propagação convencional é difícil, devido à presença do endocarpo extremamente lignificado. Embora o hipoclorito de sódio (NaOCl) seja amplamente utilizado para a desinfestação superficial, existem poucos relatos de seus efeitos sobre o crescimento do explante. Neste trabalho, o objetivo foi estudar o efeito de diferentes pHs e períodos de exposição de sementes de B. intermedia em uma solução de NaOCl. As sementes foram submetidas a diferentes tempos de exposição (1, 5 e 10 minutos) numa solução de NaOCl com diferentes pHs (5, 7, 10 e 12) e, após o tratamento com hipoclorito de sódio, os embriões foram inoculados em meio WPM com a concentração de 50% de sais sem sacarose, ágar a 0,5% e pH 5,8 e, depois de 75 dias de cultivo, o crescimento das plântulas foi avaliado. A utilização de NaOCl é eficaz na desinfestação de sementes de B. intermedia, independente da variação do pH ou dos períodos de exposição. A porcentagem de plântulas normais, o comprimento da parte aérea e o número de folhas são positivamente afetados pela utilização de solução de NaOCl a pH de 8,5-8,9 ou pelo aumento do período de exposição, enquanto o número de raízes é afetado apenas pelo aumento do período de exposição na solução NaOCl.

Byrsonima intermedia A. Juss., is a medicinal and fruit plant of the Cerrado in which the conventional propagation is difficult due to the presence of extremely lignified endocarps. Although sodium hypochlorite (NaOCl) is widely used in the surface decontamination, there are few reports of its effects on explant growth. The aim of this work was to study the effect of different pH and exposure periods of B. intermedia seeds to a NaOCl solution. Seeds were subjected to different exposure periods (1, 5 and 10 minutes) to a NaOCl solution at different pH (5, 7, 10 and 12) and after treatment with NaOCl, embryos were inoculated in a WPM medium with 50% concentration of salts without sucrose, 0.5% agar and pH 5.8 and after 75 days of culture the growth of seedlings was evaluated. The use of NaOCl is effective in the decontamination of B. intermedia seeds, independent of pH variation and exposure periods and the parameters such as percentage of normal seedlings, shoot length, and number of leaves are positively affected by the use of NaOCl solution at pH 8.5-8.9 and by increasing the exposure period, however, the number of roots is affected only by increasing the exposure period in the NaOCl solution.

Ecotoxicity of raw and treated effluents generated by a veterinary pharmaceutical company: a comparison of the sensitivities of different standardized tests.

Pharmaceutical effluents have recently been recognized as an important contamination source to aquatic environments and the toxicity related to the presence of antibiotics in effluents has attracted great attention. Conventionally, these effluents have been treated using physico-chemical and aerobic biological processes, usually with low rates of pharmaceuticals removal. Due to the complexity of effluents, it is impossible to determine all pharmaceuticals and their degradation products using analytical methods. Ecotoxicity tests with different organisms may be used to determine the effect level of effluents and thus their environmental impacts. The objective of this work was to compare the sensitivities of five ecotoxicity tests using aquatic and terrestrial organisms to evaluate the toxicity of effluents from the production of veterinary medicines before and after treatment. Raw and chemically treated effluent samples were highly toxic to aquatic organisms, achieving 100,000 toxic units, but only few of those samples presented phytotoxicity. We observed a reduction in the toxicity in the biologically treated effluent samples, which were previously chemically pre-treated, however the toxicity was not eliminated. The rank of test organisms' reactions levels was: Daphnia similis > Raphidocelis subcapitata > Aliivibrio fischeri > Allium cepa ~ Lactuca sativa. Effluent treatment employed by the evaluated company was only partially efficient at removing the effluent toxicity, suggesting potential risks to biota. The acute toxicity test with D. similis proved to be the most sensitive for both raw and treated effluents and is a suitable option for further characterization and monitoring of pharmaceutical effluents.

Variation of karyotype and nuclear DNA content among four species of Plectranthus L' Héritier, 1788 (Lamiaceae) from Brazil.

Plectranthus is a genus which includes species of ornamental and medicinal potential. It faces taxonomic problems due to aggregating species previously belonging to the genus Coleus, a fact that has contributed to the existence of various synonymies. The species Plectranthus amboinicus, Plectranthus barbatus, Plectranthus grandis and Plectranthus neochilus are included in this context. Some authors consider Plectranthus barbatus and Plectranthus grandis as synonyms. The present work was carried out with the aim of comparing plants of the above-mentioned species, originating from different localities in Brazil, with regards to chromosome number and karyotypic morphology, correlated to the nuclear DNA content. There was no variation in chromosome number among plants of the same species. Plectranthus amboinicus was the only species to exhibit 2n=34, whereas the others had 2n=30. No karyotypic differences were found among the plants of each species, except for Plectranthus barbatus. The plants of the Plectranthus species revealed little coincidence between chromosome pairs. The nuclear DNA content allowed grouping Plectranthus amboinicus and Plectranthus neochilus, with the highest mean values, and Plectranthus grandis and Plectranthus barbatus with the lowest ones. Differences in DNA amount among the plants were identified only for Plectranthus barbatus. These results allow the inference that the populations of Plectranthus amboinicus and Plectranthus neochilus present coincident karyotypes among their plants, and Plectranthus grandis is probably a synonym of Plectranthus barbatus.

Growth curve, biochemical profile and phytochemical analyses in calli obtained from the procambium segments of Bacupari

Garcinia brasiliensis, popularly known as Bacupari, is native to the Amazon and commonly used in folk medicine for its therapeutic properties. This plant is rich in bioactive compounds like benzophenones. However, there are no works about the in vitro establishment and achievement of secondary metabolites in this plant. Thus, the aim of this work was to determine the growth curve and to perform the biochemical and phytochemical analyses in calli obtained from the procambium segments of Bacupari. The growth curve of calli followed a sigmoidal pattern, with four distinct phases (lag, exponential, linear, deceleration). Total soluble sugars were higher on the inoculation day and the reducing sugars on the 20 th day. Amino acids increased from the 60 th day up to the stabilization on the 120 th day. The protein content varied, but it seemed to be related to the amino acids metabolism. The phytochemical screening showed the presence of phenolic and flavonoid compounds in the calli and the HPLC analysis allowed the identification of Fukugetin, Guttiferone A and 7-epiclusianone.