Degradation of crude oil-associated polycyclic aromatic hydrocarbons by marine-derived fungi.

One of the major environmental concerns today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Crude oil is a complex mixture of hydrocarbons like alkanes, naphthene and polycyclic aromatic hydrocarbons (PAHs). PAHs are known to be highly toxic to humans and animals due to their carcinogenic and mutagenic effects. PAHs are environmentally recalcitrant due to their hydrophobicity which makes them difficult to degrade, thus making them persistent environmental contaminants. The mechanical and chemical methods in practice currently to remove hydrocarbon contaminants have limited effectiveness and are expensive. Bioremediation is a cost-effective technology for treating hydrocarbon-contaminated sites as it results in the complete mineralisation of the pollutant. This study demonstrates the degradation of crude oil and associated PAHs using ten fungal cultures isolated from the aquatic environment. The current study reported a 98.6% and 92.9% reduction in total PAHs in crude oil by Fusarium species, i.e. isolate NIOSN-T4 and NIOSN-T5, respectively. The fungal isolate, NIOSN-T4, identified as Fusarium equiseti, showed maximum PAH degradation efficiency of LMW PAHs 97.8%. NIOSN-M126, identified as Penicillium citrinum, exhibited a 100% removal of HMW PAHs. Microorganisms possess an untapped potential for various applications in biotechnology, and the current study demonstrated the potential of marine fungi for use in the bioremediation of xenobiotic hydrocarbons in the environment. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03753-2.

Mechanisms regulating the airborne survival of Klebsiella pneumoniae under different relative humidity and temperature levels.

In this study, Klebsiella pneumoniae was suspended in synthetic saliva in a nebulizer (N0 ) and nebulized for 5 min (N5 ) into an aerosol chamber and further prolonged in the aerosolization phase for 15 min (A15 ) under four different conditions: 20°C, 50% relative humidity (RH); 20°C, 80% RH; 30°C, 50% RH; and 30°C, 80% RH. Samples were collected at N0 , N5 , and A15 , then subjected to survival analysis and comparative transcriptomic analysis in order to help elucidate the underlying mechanisms of airborne survival. Survival analysis shows that a higher humidity and lower temperature were favorable for the airborne survival of K. pneumoniae, and the effect of RH was more remarkable at 20°C than that at 30°C. The RNA-seq results show that during the nebulization phase (N0 vs. N5 ), a total number of 201 differentially expressed genes (DEGs) were identified (103 downregulated and 98 upregulated). Comparison between nebulization and aerosolization phases (N5 vs. A15 ) indicates up to 132 DEGs, with 46 downregulated and 86 upregulated. The most notable groups of genes are those involved in cellular remodeling, metabolism and energy processes. Alarmingly, the mbl gene, which encodes antibiotic resistance in K. pneumoniae, was upregulated during the suspension phase under all the tested conditions. This study provides insights into the control of airborne transmitted diseases.

In-Cabin Air Quality during Driving and Engine Idling in Air-Conditioned Private Vehicles in Hong Kong.

Many people spend lengthy periods each day in enclosed vehicles in Hong Kong. However, comparably limited data is available about in-cabin air quality in air-conditioned private vehicles, and the car usage that may affect the air quality. Fifty-one vehicles were tested for particulate matter (PM0.3 and PM2.5), total volatile organic compounds (TVOCs), carbon monoxide (CO), carbon dioxide (CO2), airborne bacteria, and fungi levels during their routine travel journey. Ten of these vehicles were further examined for PM0.3, PM2.5, TVOCs, CO, and CO2 during engine idling. In general, during driving PM2.5 levels in-cabin reduced overtime, but not PM0.3. For TVOCs, 24% vehicles exceeded the recommended Indoor Air Quality (IAQ) level in offices and public places set by the Hong Kong Environmental Protection Department. The total volatile organic compounds (TVOC) concentration positively correlated with the age of the vehicle. Carbon monoxide (CO) levels in all of the vehicles were lower than the IAQ recommendation, while 96% vehicles exceeded the recommended CO2 level of 1000 ppmv; 16% vehicles >5000 ppmv. Microbial counts were relatively low. TVOCs levels at idle engine were higher than that during driving. Although the time we spend in vehicles is short, the potential exposure to high levels of pollutants should not be overlooked.

Bioremediation potential of hydrocarbon-utilizing fungi from select marine niches of India.

Ten fungal isolates with an ability to degrade crude oil were isolated from select marine substrates, such as mangrove sediments, Arabian Sea sediments, and tarballs. Out of the ten isolates, six belonged to Aspergillus, two to Fusarium and one each to Penicillium and Acremonium as identified using ITS rDNA sequencing. The selected ten fungal isolates were found to degrade the long-chain n-alkanes as opposed to short-chain n-alkanes from the crude oil. Mangrove fungus #NIOSN-M126 (Penicillium citrinum) was found to be highly efficient in biodegradation of crude oil, reducing the total crude oil content by 77% and the individual n-alkane fraction by an average of 95.37%, indicating it to be a potential candidate for the development into a bioremediation agent.