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1.
PLoS One ; 5(5): e10677, 2010 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-20505757

RESUMO

BACKGROUND: Located in the Dutch Windward Islands, Saba Bank is a flat-topped seamount (20-45 m deep in the shallower regions). The primary goals of the survey were to improve knowledge of biodiversity for one of the world's most significant, but little-known, seamounts and to increase basic data and analyses to promote the development of an improved management plan. METHODOLOGY/PRINCIPAL FINDINGS: Our team of three divers used scuba to collect algal samples to depths of 50 m at 17 dive sites. Over 360 macrophyte specimens (12 putative new species) were collected, more than 1,000 photographs were taken in truly exceptional habitats, and three astonishing new seaweed community types were discovered. These included: (1) "Field of Greens" (N 17 degrees 30.620', W 63 degrees 27.707') dominated by green seaweeds as well as some filamentous reds, (2) "Brown Town" (N 17 degrees 28.027', W 63 degrees 14.944') dominated by large brown algae, and (3) "Seaweed City" (N 17 degrees 26.485', W 63 degrees 16.850') with a diversity of spectacular fleshy red algae. CONCLUSIONS/SIGNIFICANCE: Dives to 30 m in the more two-dimensional interior habitats revealed particularly robust specimens of algae typical of shallower seagrass beds, but here in the total absence of any seagrasses (seagrasses generally do not grow below 20 m). Our preliminary estimate of the number of total seaweed species on Saba Bank ranges from a minimum of 150 to 200. Few filamentous and thin sheet forms indicative of stressed or physically disturbed environments were observed. A more precise number still awaits further microscopic and molecular examinations in the laboratory. The expedition, while intensive, has only scratched the surface of this unique submerged seamount/atoll.


Assuntos
Biodiversidade , Eucariotos/fisiologia , Água do Mar , Eucariotos/classificação , Geografia , Antilhas Holandesas , Especificidade da Espécie
2.
J Med Virol ; 61(1): 117-24, 2000 May.
Artigo em Inglês | MEDLINE | ID: mdl-10745243

RESUMO

To determine the importance of Norwalk-like viruses (NLVs) as pediatric pathogens in a developing country, the seroprevalence and seroincidence of this group of viruses in a cohort of children less than 4 years of age in an urban shantytown in northeastern Brazil was examined. Serum samples were collected approximately every 6 months from 135 children who were surveyed three times each week for diarrhea and vomiting. NLV IgG was measured by an enzyme immunosorbent assay (EIA) with recombinant Norwalk virus capsid protein. Overall NLV seroprevalence was 71%, and the overall NLV seroconversion rate was 0.7 seroconversions per child-year. The highest age-specific NLV seroconversion rate (0.8 seroconversions per child-year) was observed in the 13-24-month age group. For all study children, the incidence of diarrhea and vomiting was significantly greater (P < 0.01) during time periods spanned by serum pairs that indicated NLV seroconversion compared with time periods without NLV seroconversion. However, NLV seroconversion was not associated with gastrointestinal symptoms during the first year of life.


Assuntos
Infecções por Caliciviridae/epidemiologia , Proteínas do Capsídeo , Capsídeo/imunologia , Brasil/epidemiologia , Infecções por Caliciviridae/imunologia , Infecções por Caliciviridae/patologia , Pré-Escolar , Diarreia/epidemiologia , Diarreia/virologia , Feminino , Gastroenterite/epidemiologia , Gastroenterite/virologia , Humanos , Técnicas Imunoenzimáticas , Incidência , Lactente , Masculino , Estudos Prospectivos , Estudos Soroepidemiológicos , Testes Sorológicos , Vômito/epidemiologia , Vômito/virologia
3.
J Med Virol ; 58(4): 426-34, 1999 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10421412

RESUMO

"Norwalk-like viruses" (NLVs) are a common cause of epidemic gastroenteritis in adults and children in developed countries. However, little is known about the role of NLVs in endemic pediatric gastroenteritis in developing countries. We sequenced Genogroup I and II NLV reverse transcription-polymerase chain reaction (RT-PCR) products from an 81-nucleotide region of the viral RNA polymerase gene to examine the molecular epidemiology of NLV infection in children younger than 5 years of age in Forteleza, Ceará, Brazil. NLV-positive PCR products were obtained from stool specimens collected over a 16-month period (1990-1991) from diarrhea cases and controls in a cohort of 120 children in an urban shantytown and from a study in the same city of hospitalized children with persistent diarrhea. Eight unique strains were detected in 15 specimens from 10 cohort children and in two hospital specimens. Nucleotide identity between the strains (5 Genogroup I, 3 Genogroup II) ranged from 63% to 88%. We designated these strains BraV1-8, for Brazil virus 1-8. The degree of genomic diversity of NLV strains we identified in this cohort during a short time period suggests multiple foci of infection within the community. Furthermore, sequence analysis of strains from two children with multiple symptomatic NLV infections indicates that infection with one strain was not protective against subsequent infection with a different strain in the same genogroup. These findings have implications for vaccine development and the prevention of pediatric gastroenteritis in developing countries.


Assuntos
Infecções por Caliciviridae/virologia , Variação Genética , Genoma Viral , Vírus Norwalk/genética , Brasil/epidemiologia , Infecções por Caliciviridae/sangue , Infecções por Caliciviridae/epidemiologia , Estudos de Coortes , Fezes/virologia , Gastroenterite/virologia , Humanos , Lactente , Vírus Norwalk/classificação , Vírus Norwalk/imunologia , Filogenia , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de Sequência , Análise de Sequência de DNA , Testes Sorológicos
4.
Am J Gastroenterol ; 92(10): 1861-6, 1997 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-9382053

RESUMO

OBJECTIVES: To determine the relative effects of AIDS-related diarrhea with or without cryptosporidiosis and microsporidiosis on intestinal function and injury. METHODS: We studied 40 HIV-infected patients (20 with and 20 without diarrhea) and 13 healthy volunteers, using the differential urinary excretion of ingested lactulose and mannitol as respective markers of barrier disruption and overall villous surface area. We also examined them for fecal leukocytes, lactoferrin, and alpha 1-antitrypsin. Fasting subjects drank test solution containing lactulose (5 g) and mannitol (1 g). Urine was collected for 5 h and tested for sugars by high-performance liquid chromatography with pulsed amperometric detection. RESULTS: HIV-positive patients with diarrhea had a 2.8-fold higher lactulose:mannitol excretion ratio (L:M) than HIV-positive patients without diarrhea (p = 0.01) and 10.4-fold higher than healthy volunteers (p = 0.004). This was accounted for by a 1.5- to 3.1-fold higher rate of lactulose excretion by HIV patients with diarrhea than by those without diarrhea or by healthy volunteers. Mannitol excretion was 32-55% less in patients with diarrhea than in those without diarrhea or in healthy volunteers. Patients with cryptosporidial diarrhea had a nearly 6-fold higher L:M ratio than those without diarrhea (p < 0.001) and nearly 3-fold higher than those with non-cryptosporidial diarrhea (p = 0.02). One patient with microsporidial infection had a nearly 3-fold higher L:M ratio than controls without diarrhea. Alpha 1-Antitrypsin was positive in 40% of HIV-positive patients with cryptosporidial infections and none of 12 HIV-positive patients with non-cryptosporidial diarrhea. Fecal lactoferrin or leukocytes were increased in all HIV patients with diarrhea. CONCLUSION: HIV infection is associated with intestinal dysfunction and injury, even in patients who do not have diarrhea. However, those with diarrhea, especially with cryptosporidiosis or microsporidiosis, have even greater disruption of intestinal barrier function with potentially important nutritional consequences.


PIP: The effects of AIDS-related diarrhea--with and without cryptosporidiosis and microsporidiosis--on intestinal function and injury were studied in 40 AIDS patients and 13 healthy volunteers from Fortaleza, Brazil. The differential urinary excretion of ingested lactulose and mannitol was used as a marker of barrier disruption and overall villous surface area. HIV-infected patients with diarrhea had a 2.8-fold higher lactulose to mannitol excretion ratio than HIV-positive patients without diarrhea and a 10.4-fold higher ratio than healthy volunteers. Moreover, those with crypotosporidial infection had a lactulose to mannitol ratio almost 6-fold greater than those without diarrhea and nearly 3-fold higher than those with non-cryptosporidial diarrhea. This effect involved both decreased mannitol excretion (decreased intestinal absorptive area) and increased lactulose excretion (mucosal barrier disruption). The single patient with microsporidial infection had a nearly 3-fold higher ratio than healthy volunteers. Alpha1-antitrypsin tests were positive in two of five (40%) HIV-positive patients with cryptosporidial infections compared with none of 12 HIV-infected patients with non-cryptosporidial diarrhea. These findings confirm that HIV infection is associated with profound intestinal dysfunction and injury, even in those without diarrhea. Disruption of the intestinal barrier is even greater, however, in HIV-infected patients with cryptosporidial diarrhea, with potential nutritional consequences.


Assuntos
Infecções Oportunistas Relacionadas com a AIDS/metabolismo , Infecções Oportunistas Relacionadas com a AIDS/patologia , Criptosporidiose/metabolismo , Criptosporidiose/patologia , Diarreia/metabolismo , Diarreia/patologia , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Adolescente , Adulto , Criança , Feminino , Humanos , Enteropatias Parasitárias/metabolismo , Enteropatias Parasitárias/patologia , Lactulose/metabolismo , Masculino , Manitol/metabolismo , Permeabilidade , Estudos Prospectivos
5.
Pediatr Infect Dis J ; 16(5): 504-7, 1997 May.
Artigo em Inglês | MEDLINE | ID: mdl-9154546

RESUMO

OBJECTIVE: To study the etiologic role of toroviruses as a cause of gastroenteritis in humans. METHODS: The design was a case-control study. We compared the rate of torovirus detection in fecal specimens from a selection of children with acute or persistent diarrhea and controls without diarrhea from a study of childhood diarrhea in an urban Brazilian slum. Stool samples were coded and tested in a blinded fashion for the presence of torovirus antigen by enzyme-linked immunosorbent assay, other enteropathogens, toxins and fecal leukocytes. RESULTS: Thirty-three children with acute diarrhea, 41 children with persistent diarrhea and 17 controls were enlisted in the study. Torovirus antigen was detected in 9 (27%) samples from children with acute diarrhea, 11 (27%) samples from children with persistent diarrhea and none of the samples from controls (P < 0.05). In addition the presence of enteroaggregative E. coli was associated with persistent diarrhea and the presence of Cryptosporidium oocysts was common although not significant (P = 0.08); torovirus and Cryptosporidium occurred in different subsets of samples, whereas torovirus and enteroaggregative Escherichia coli were commonly found in combination. CONCLUSIONS: These data indicate that toroviruses, alone or in combination with enteroaggregative E. coli, may play a pathogenic role in acute and possibly persistent diarrhea. Further studies are warranted to determine the etiologic role of toroviruses in gastroenteritis.


Assuntos
Antígenos Virais/análise , Diarreia/microbiologia , Infecções por Escherichia coli/complicações , Fezes/virologia , Gastroenterite/microbiologia , Infecções por Torovirus/complicações , Torovirus/imunologia , Doença Aguda , Animais , Brasil/epidemiologia , Pré-Escolar , Doença Crônica , Cryptosporidium/isolamento & purificação , Diarreia/epidemiologia , Ensaio de Imunoadsorção Enzimática , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Fezes/microbiologia , Feminino , Gastroenterite/epidemiologia , Humanos , Incidência , Lactente , Estudos Longitudinais , Masculino , Infecções por Torovirus/epidemiologia , Infecções por Torovirus/virologia , População Urbana
8.
Am J Trop Med Hyg ; 51(1): 1-10, 1994 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-8059906

RESUMO

A cluster-sampling, cross-sectional study was conducted for assessing the prevalence of Cryptosporidium infection in children less than 16 years of age from three villages, Dondian, Linshan, and Fuziyin, in rural Anhui in eastern China. Among 320 apparently healthy children less than 10 years of age from Dondian who had stool specimens collected, cryptosporidial oocysts were found in stools of three children from Dondian, and no positive specimens were found in 239 children studied from Linshan. In addition, a total of 610 serum samples from children in these three villages were tested for specific IgG antibody to Cryptosporidium with an enzyme-linked immunosorbent assay (ELISA) and the prevalence rates were 42.3%, 51.7%, and 57.5%, respectively, in Dondian, Linshan, and Fuziyin. Seroprevalence increased progressively with age. No detectable antibody was found in infants between two and six months of age, and seropositivity steadily increased after one year of age. Among 36 sera from adults 15-60 years of age without diarrheal illness in Huanglu villages of rural Chaohu, 50% (18 of 36) were positive. As expected, a good correlation was found in the specific IgG antibody between the paired serum specimens from 30 matched mother-neonates who showed transplacental transfer of IgG. However, little or no IgM antibody was seen in the neonates even though several mothers had a positive anticryptosporidial IgM enzyme-linked immunoassay result. Forty randomly selected serum samples from children less than four years of age in a similarly impoverished semiurban community in Fortaleza, Brazil, where the majority of households also have pit toilets and shared community water supplies and 172 serum samples from patients one month to 29 years of age admitted to the University of Virginia Hospital without diarrhea were also examined. In Fortaleza, almost all children acquired antibody by their second year of life, demonstrating the high prevalence of this infection. In rural Anhui, only about half the children were infected by 5-7 years of age. The overall prevalence rate (16.9%) of seropositivity among children and young adults in Virginia was much lower than in China and Brazil. These results indicate that cryptosporidial infection is ubiquitous, and is highly endemic in these impoverished communities. The difference between China and Brazil may reflect earlier weaning, hygiene practices, poorer water or sanitation, multiple siblings in family and geographic environment in Brazil.


Assuntos
Anticorpos Antiprotozoários/sangue , Criptosporidiose/epidemiologia , Cryptosporidium/imunologia , Adolescente , Adulto , Fatores Etários , Animais , Brasil/epidemiologia , Criança , Pré-Escolar , China/epidemiologia , Estudos Transversais , Ensaio de Imunoadsorção Enzimática , Fezes/parasitologia , Feminino , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Lactente , Masculino , Prevalência , Reprodutibilidade dos Testes , População Rural
9.
Pediatr Infect Dis J ; 10(10): 746-51, 1991 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-1658721

RESUMO

We examined stools from 40 children with persistent diarrhea (duration, 14 days or more), from 50 children with acute diarrhea and from 38 control children to determine infectious etiologies for persistent diarrhea in Goncalves Dias, an urban favela (slum) in Fortaleza, Ceara, Brazil. Children with persistent diarrhea and children with acute diarrhea had similar rates of isolation of routine viral, bacterial and parasitic enteric pathogens. Routine pathogens were identified in at least 20% of cultures done more than 14 days into the diarrheal illness. We examined Escherichia coli isolated from these stools for adherence potential. Enteroaggregative E. coli were isolated significantly more often from children with persistent diarrhea than from control children or children with acute diarrhea (P less than 0.05). E. coli with hemagglutination patterns suggestive of adherence pili were also isolated more often from children with persistent diarrhea than from children with acute diarrhea (38% vs. 18%; P less than 0.05). Enterotoxigenic E. coli were isolated in combination with rotavirus more often from children with persistent diarrhea than from children with acute diarrhea. E. coli which were hydrophobic or exhibited hemagglutination were also seen more often in association with Giardia in children with persistent diarrhea. These findings suggest that the etiology of persistent diarrhea in children is complex and that the aggregative E. coli are associated with prolonged diarrheal illness. Although routine diarrheal pathogens may be present for more than 14 days, combinations of pathogens, including E. coli with adherence potential, may also contribute to prolonged diarrheal disease.


Assuntos
Aderência Bacteriana , Diarreia/microbiologia , Infecções por Escherichia coli/microbiologia , Escherichia coli/patogenicidade , Animais , Brasil , Pré-Escolar , Cryptosporidium/isolamento & purificação , Diarreia/parasitologia , Escherichia coli/imunologia , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/complicações , Fezes/microbiologia , Fezes/parasitologia , Feminino , Giardíase/complicações , Hemaglutinação , Humanos , Lactente , Masculino , Áreas de Pobreza , Coelhos , Rotavirus/isolamento & purificação , População Urbana
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