Emergent contaminants: Endocrine disruptors and their laccase-assisted degradation - A review.

Herein, an effort has been made to highlight the trends of the state-of-the-art of laccase-assisted degradation of emerging contaminants at large and endocrine disruptors in particular. Since first described in the 19th century, laccase has received particular interest for inter- and multidisciplinary investigations due to its uniqueness and remarkable biotechnological applicability. There has always been a paramount concern over the widespread occurrences of various pollutant types, around the globe. Therefore, pollution free processes are gaining ground all over the world. With ever increasing scientific knowledge, socioeconomic awareness, human health-related issues and ecological apprehensions, people are more concerned about the widespread environmental pollutants. In this context, the occurrences of newly identified pollutants so-called "emerging contaminants - ECs" in our main water bodies is of continued and burning concern worldwide. Undoubtedly, various efforts have already been made to tackle this challenging ECs concern though using different approaches including physical and chemical, however, each has considerable limitations. In this review, we present information on how laccase-assisted approach can change this limited tendency of physical and chemical based approaches. A special focus has been given to the laccase-assisted systems including pristine laccase, laccase-mediator catalyzed system and immobilized-laccase catalyzed system that promotes the endocrine disruptors removal. Towards the end, a list of outstanding questions and research gaps are given that can pave the way for future studies.

Potentialities of active membranes with immobilized laccase for Bisphenol A degradation.

Herein, we report the development of immobilized laccase based membrane bioreactor as a novel bio-catalytic system for the degradation of emerging endocrine disruptor i.e., Bisphenol A. Two laccase forms i.e. (1) in-house isolated and purified from an indigenous white-rot fungi Pycnoporus sanguineus (CS43) and (2) Trametes versicolor (commercial laccase from Sigma-Aldrich®) were immobilized on a multi-channel ceramic membrane (1.4µm in diameter) using 4% glutaraldehyde as a cross-linking agent. The immobilization yield and bisphenol A degradation activities of immobilized laccases were recorded at various pH levels. The surface topographies of immobilized-laccase membranes were accessed by scanning electron microscopy. In this study, 100% degradation of bisphenol A (20mg/L) was achieved in less than 24h in the presence of laccase from P. sanguineus (CS43) (620.55±14.85U/L) and T. versicolor (620.55±14.85U/L). The enzymes showed an optimal activity at pH 5 and 5.4 with a degradation rate of 204.8±1.8 and 79.0±0.1µmol/min/U for P. sanguineus (CS43) and T. versicolor, respectively. In conclusion, the highest immobilization of unit per square centimeter and efficient degradation potentiality strongly recommend the newly developed immobilized laccase based membrane bioreactor as a novel tool to tackle emerging contaminants degradation issues.