[Pregnancy outcomes in women with gestational diabetes: specific subgroups might require increased attention]. / Pacientky s obezitou, hypertenzí a nutností aplikace inzulinu pri diagnóze gestacní diabetes mellitus vyzadují zvýsenou porodnickou péci.

OBJECTIVES: To compare peri-partal parameters between two groups of pregnant women - with and without gestational diabetes mellitus (GDM), to correlate degree of glucose abnormality with incidence of peri-partal morbidity and, finally, to analyse the potential effect of comorbidities (i.e. obesity, hypertension, thyreopathy, polycystic ovary syndrome, trombophylia, anemia, allergy, smoking) on pregnancy outcomes. DESIGN: Epidemiological observational "case-control" study. SETTING: Department of Obstetric and Gynaecology, Faculty Hospital Brno; Department of Internal Medicine, Diabetes Centre, Faculty Hospital Brno; Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno. METHODS: The study comprised 432 pregnant women (364 with GDM diagnosis, 68 healthy controls) followed during a period 2011-2013. GDM was diagnosed by oral glucose tolerance test in 24-28th week of gestation (by fasting plasma glucose >5,6 mmol/l or >8,8 mmol/l in 60th min or >7,8 mmol/l in 120th min post-75g glucose load). Following peri-partal parameters were studied: ultrasonographic examination before delivery, a date of delivery, length of childbirth, induction, perinatal complications, post-delivery complications, section, abnormity in pH, base excess, Apgar score, birth weight. RESULTS: Subjects with GDM had significantly increased rate of labour induction compared to healthy controls (P = 0.0035, chi-square test). Subgroup of GDM women classified as having a higher risk for adverse perinatal outcomes by a definition of Czech Obstetric and Gynaecology Society had significantly more labour inductions, more sections and instrumental deliveries. New-borns of those mothers had significantly more common worse perinatal outcomes (Apgar score and macrosomia). CONCLUSION: Based on our data risk stratification of GDM subjects according to Czech Obstetric and Gynaecology Society appears relevant and justified.

Association between three single nucleotide polymorphisms in eotaxin (CCL 11) gene, hexanucleotide repetition upstream, severity and course of coronary atherosclerosis.

The impact of three single-nucleotide polymorphisms in eotaxin (SCYA11) gene promoter (-426C>T and -384A>G) and first exon (67G>A) and recently described hexanucleotide (GAAGGA)(n) 10.9 kb upstream on coronary atherosclerosis was investigated. Elective coronary angiography of 1050 consecutive subjects was performed. All patients were genotyped for the three SNPs. In a subset of the first 472 samples, the number of (GAAGGA)(n) repetitions was determined. For further evaluation, short and long variants were distinguished; the borderline corresponded with the median value of all alleles: ≤8 repetitions were considered as short sequence, ≥9 repetitions as long. Patients with bronchial asthma or insignificant atherosclerosis were excluded; the remaining group of 933 subjects was further investigated. Patients were grouped according to the form of CAD (ACS vs. stable angina) and the number of diseased vessels. The GG variant of 67 G>A polymorphism was associated with acute form of CAD compared to stable angina (p=0.0011, p(corr.)=0.013). The number of (GAAGGA)(n) repetitions in our set of patients ranged from 3 to 12. There were no subjects with 4 or 5 repetitions. The frequency of short repetition alleles increased with the number of affected vessels (1 vs. 3 diseased vessels: p=0.0043, p(corr)=0.034). In our study, the (GAAGGA)(n) hexanucleotide was associated with the severity of CAD. The 67 GG was associated with acute form of CAD. None of the two SNPs in eotaxin promoter had any relation to CAD. The number of (GAAGGA)(n) repetitions can thus be a novel genetic marker of the extent of CAD.

[Influence of calcium hydroxide under filling cements]. / Die Beeinflussung von Kalziumhydroxid durch Unterfüllungszemente.

In the paper lying before the authors estimate changes of pH-values of the Calcium hydroxide (Calxyd) after contact with a zinc oxide phosphate cement (Adhesor), a polyacrylate cement (Adhesor-Carboxy) and a zinc oxide eugenol cement (Caryosan). The last shoved to be best qualified for over capping of pasty preparation with calcium hydroxide.

[Biocompatibility of a chromium-cobalt alloy studied in vitro]. / In-vitro-Untersuchungen zur Biokompatibilität einer Kobalt-Chrom-Legierung.

The authors followed the effect of chromcobalt alloy on human cells cultured in vitro. They found out that corrosion increases the cytotoxicity of this alloy, meanwhile the perfectly laboratory elaboration, especially polishing of substitution decreases the cytotoxicity. We recommend for the complex cytotoxicity review, to test the metals and alloys also after laboratory elaboration, it means in the condition it is passed on to the patient.

[Simple stomatosurgical operations in patients with anticoagulant therapy]. / Jednoduché stomatochirurgické výkony u nemocných s antikoagulacní terapií.

The authors give an account of contemporary therapeutic procedures in stomatosurgery in patients with anticoagulant treatment and draw attention to situations where it is better to treat these patients in the central department of the health community dental surgeon.