Cold plasma application to fresh green leafy vegetables: Impact on microbiology and product quality.

Fresh green leafy vegetables (FGLVs) are consumed either garden-fresh or by going through very few simple processing steps. For this reason, foodborne diseases that come with the consumption of fresh products in many countries have prioritized the development of new and reliable technologies to reduce food-related epidemics. Cold plasma (CP) is considered one of the sustainable and green processing approaches that inactivate target microorganisms without causing a significant temperature increase during processing. This review presents an overview of recent developments regarding the commercialization potential of CP-treated FGLVs, focusing on specific areas such as microbial inactivation and the influence of CP on product quality. The effect of CP differs according to the power of the plasma, frequency, gas flow rate, application time, ionizing gases composition, the distance between the electrodes and pressure, as well as the characteristics of the product. As well as microbial decontamination, CP offers significant potential for increasing the shelf life of perishable and short-shelf-life products. In addition, organizations actively involved in CP research and development and patent applications (2016-2022) have also been analyzed.

Inhibition effect of belzalkonium chloride treatment on growth of common food contaminating fungal species.

The improvement of disinfection applications for hard contact surfaces in food processing is critical for the control and prevention of disease-causing and food spoilage microorganisms. The objective in this study was to determine the efficiency of the antifungal agent benzalkonium chloride on growth and/or spore germination of postharvest fruit pathogenic fungi (Aspergillus spp., Penicillum spp., and Alternae alternate) in vitro. The benzalkonium chloride was found to be active against all fungal species but to a different extent. Addition of ethylenediamine-tetraacetic acid and its sodium salt increased the sensitivity of fungi to benzalkonium chloride. Thus, integrated washing and sanitizing with benzalkonium chloride or homologous surface active compounds combined with ethylenediamine-tetraacetic acid and its sodium salt is promising fungicide candidates for reducing fungal contamination of storage.

Plant molecular farming: opportunities and challenges.

Production of foreign molecules in transgenic plants is anticipated to be an alternative to already established, microbial or animal expression systems with lower production costs. This article reviews the different technologies and approaches currently used to produce economically interesting molecules in plants or plant cell cultures, to evaluate their technical feasibility and economic implications, and to assess the potential socioeconomic and environmental impacts deriving from the adoption of molecular farming products.

Elimination of Aspergillus parasiticus from nut surface with low pressure cold plasma (LPCP) treatment.

Low pressure cold plasma (LPCP) using air gases and sulfur hexafluoride (SF(6)) was developed and tested for anti-fungal efficacy against Aspergillus parasiticus on various nut samples. Artificially A. parasiticus contaminated hazelnuts, peanuts, and pistachio nuts were treated with air gases plasma and SF(6) plasma for up to 20 min duration. The sterilizing effect of LPCP on A. parasiticus was higher during the early treatment period than the later treatment period. Air gases plasma treatment for 5 min resulted in 1-log reduction of A. parasiticus and a further 5 min treatment resulted in additional 1-log reduction. SF(6) plasma application was more effective resulting in approximately a 5-log decrease in fungal population for the same duration. When effectiveness of plasma treatment against aflatoxins were tested, 20 min air gases plasma treatment resulted in a 50% reduction in total aflatoxins (AFB1, AFB2, AFG1, and AFG2), while only a 20% reduction in total aflatoxin was observed after 20 min SF(6) plasma treatment. In this study, a rapid, functional clean-up method for the elimination of aflatoxin producing fungus from shelled and unshelled nuts was investigated as a suitable fungal decontamination method.

Decontamination of grains and legumes infected with Aspergillus spp. and Penicillum spp. by cold plasma treatment.

The objective of this study was to determine the efficacy of a self-designed low pressure cold plasma (LPCP) system using air gases or SF6. For the inactivation and/or elimination of two pathogenic fungi, Aspergillus spp. and Penicillum spp. artificially contaminated on seed surface. The plasma decontamination process was performed by batch process in vacuum chamber, using gas injection followed by plasma discharge for the duration of 5-20 min. The plasma treatment reduced the fungal attachment to seeds below 1% of initial load depending on the initial contamination level, while preserving germination quality of the seed. A significant reduction of 3-log for both species was achieved within 15 min of SF6 plasma treatment time. Air gases plasma and SF6 plasma in particular provides an interesting surface decontamination alternative for seeds.

Characterization of beta-xylosidase enzyme from a Pichia stipitis mutant.

beta-Xylosidase production was maximal for the mutant Pichia stipitis NP54376 grown on xylan as the sole carbon source. beta-Xylosidase was purified from culture supernatant by (NH(4))(2)SO(4) precipitation and a hydrophobic interaction chromatography on phenyl sepharose. Optima of pH and temperature were 5.0 and 50 degrees C, respectively. The enzyme was inhibited by 2-mercaptoethanol (100%) and Fe(3+) (80%), and moderately affected by Cu(2+), Ag(+), NH(4)(+) and Mg(2+) and SDS. The purified xylosidase hydrolyzed xylobiose and xylo-oligosaccharides and it did not exhibit activity against cellulose, starch, maltose and cellobiose. 2.5 g l(-1) glucose repressed beta-xylosidase activity in the NP54376 strain. The K(m) and V(max) values on p-nitrophenyl-beta-xylopyranoside were 1.6 mM and 186 micromol p-nitrophenyl min(-1)mg(-1) protein, respectively. Analysis of the hydrolysis products by HPLC indicated that the major hydrolysis product is xylobiose in all the carbon sources tested.

Contemporary approaches into obesity: drugs and genes.

Obesity is a global epidemic effecting the health and life style of millions of people in both developed and developing countries. In this article, current medical treatments, recent scientific progresses toward understanding obesity, and future potentials in biotechnology applications in pharmaceutical research are reviewed in detail.