Effects of mitochondrial transplantation on chronic pressure wound healing in a human patient.

BACKGROUND AIMS: Wound healing is a multistage process that requires a concerted effort of various cell types. The intricate processes involved in the healing of wounds result in high energy requirements. Furthermore, mitochondria play a crucial role in the healing process because of their involvement in neo angiogenesis, growth factor synthesis, and cell differentiation. It is unclear how mitochondria transplantation, a promising new approach, influences wound healing. METHODS: In this study, healthy autologous mitochondria obtained from skeletal muscle were injected into chronic pressure wounds as an intervention to promote wound healing. RESULTS: Mitochondrial transplantation accelerated wound healing by reducing wound size, increasing granulation tissue, and hastening epithelialization. CONCLUSIONS: This study is the first to demonstrate the therapeutic efficacy of mitochondrial transplantation in wound healing.

The Effects of Colchicine-Impregnated Oxidized Regenerated Cellulose on Capsular Contracture.

Capsular contracture is the most common complication of breast augmentation. Oxidized regenerated cellulose can be used as a matrix for drug transport. Colchicine is an antimitotic drug that interferes with various steps of wound healing. The aim of this study was to evaluate the effects of oxidized regenerated cellulose alone or in combination with colchicine on capsular contracture. Twenty-one adult female Wistar-Albino rats were divided into 3 groups. In group 1 silicone blocks only, in group 2 oxidized regenerated cellulose-wrapped silicone blocks, and in group 3 colchicine-impregnated oxidized regenerated cellulose-wrapped silicone blocks were inserted in the dorsal region. Four weeks later, implants were removed and histopathological examination was performed. Capsular thickness, inflammatory infiltrate degree, collagen fiber organization, and myofibroblast density were evaluated. Macroscopic examination revealed a distinct capsule formation only in group 1 animals, with average measurement being 134.65 µm on histopathological examination. In groups 2 and 3 animals, no distinct capsule formation was seen. Inflammatory infiltrate degree was found to be less in groups 2 and 3 animals than in group 1 animals. Collagen fiber organization around the implants was found to be parallel and organized in group 1 animals, whereas it was random and disorganized in animals in both groups 2 and 3. High myofibroblast density was observed in animals in groups 1 and 2, while no myofibroblast was found in animals in group 3. The results of our study suggest that coating silicone implants with oxidized regenerated cellulose or with colchicine-impregnated oxidized regenerated cellulose may be effective in preventing capsular contracture.

Reconstruction of vermilion deficiencies: the running V-flap technique.

Notching, or 'whistle deformity', is a common vermilion deformity among patients with cleft lips. Vermilion volume deficiency may also be present secondary to trauma or tumour excision. Herein, we present a new flap model to repair such vermilion deformities. Six patients were included in the study. Running V-flaps prepared from the oral mucosa were used. While the underlying cause of vermilion deficiency was cleft-lip surgery in four of the patients, the indication for surgery was trauma related in two patients. In all patients, the volume deficiency was corrected. No patient exhibited scarring, colour mismatch or asymmetry. We believe that our new flap model can be a reliable alternative in secondary vermilion deficiencies.

Closure or reduction of the donor defect of a sural flap with a purse-string suture: long-term results.

We aimed to reduce the defect with a purse-string suture to minimise the deformity of the flap donor area and to assess the later efficiency at long-term follow-up. We studied 20 patients who required a sural flap for the reconstruction of defects of the lower extremity. The mean (SD) area of the donor defect was roughly 28.7 (20.3) cm(2). The mean (SD) defect area after closure with a purse-string was 8.2 (5.8) cm(2) (p < 0.001). The measurements made in the third year were 8.4 (5.3) cm(2). The purse-string can be used successfully in the distal lower extremity, the long-term cosmetic outcome is good, and the scar has not expanded since the postoperative period.

Follicular unit extraction in hair transplantation: personal experience.

Hair transplantation in the form of follicular units constitutes the cornerstone of hair-replacement surgery. Harvesting follicular units directly from the donor area by using punches is described as follicular unit extraction (FUE) technique, and it eliminates the need for excision of a hair-bearing strip. In this article, we presented our surgical approach for hair transplantation, which is based on performing follicular extraction in 2 phases: follicular unit isolation and follicular unit collection, and discussed some technical details of FUE and some advantages and disadvantages of this approach. We transplanted an average of 1651 follicular units in 1 day, 2610 follicular units in 2 days, and 3200 follicular units in 3 days.

An unusual and serious complication of topical wart treatment with monochloroacetic acid.

Numerous chemical agents are used in the topical treatment of warts. Monochloroacetic acid (MCAA) is one of these agents, which is used in low or high concentrations in most European countries. MCAA is a strong organic acid which is irritating and corrosive to the skin and has a high systemic toxicity. In addition to wart treatment, it is used for industrial purposes, such as the synthesis of certain organic chemicals. We present a case of joint deformity manifesting after the use of a preparation containing MCAA for topical wart treatment. This underlines the need to reassess the safety of MCAA use for topical wart treatment.

Effects of surgical or chemical delaying procedures on the survival of proximal predicled venous island flaps: an experimental study in rats.

To increase the survival of proximally pedicled venous island flaps, which are known to be safe only when small, we used surgical and chemical delaying procedures both individually and in combination for 7, 14, and 21 days. Survival was 1% in those flaps designated as controls, in which there was neither surgical nor chemical delay. However, it was 3%, 31%, and 68% at 7, 14, and 21 days, respectively, when surgical delay was used and 16%, 56%, and 86%, at 7, 14, and 21 days, respectively, when chemical delay was used. Survival was 32%, 84%, and 87%, at 7, 14, and 21 days when both chemical and surgical delays were used. We conclude that chemical delay is more effective than surgical delay in increasing survival of proximally pedicled venous island flaps, but the use of combined chemical and surgical delay may reduce the time required for delay.