Linfoma extranodal de células B em vesícula urinária de um cão / Extranodal B-cell lymphoma in the urinary bladder in a dog

O linfoma é uma neoplasia de alta recorrência na rotina oncológica de medicina veterinária. Pode ser classificado em linfoma Hodgking-liked, com raros casos descritos somente em felinos,e não Hodgking, sendo este segundo o mais comum, subdividido em linfomas B ou T. O objetivo deste trabalho foi relatar a conduta clínica, diagnóstica e terapêutica do caso de uma cadela, de 12 anos, sem raça definida, que manifestava disúria, prostração, dor abdominal e ao exame físico a presença de uma massa na região hipogástrica. Esta foi diagnosticada com linfoma de grandes células por meio de exames de citologia e biópsia, com solicitação do exame de imunoistoquímica que confirmou linfoma difuso de grandes células de imunofenótipo B. Sem o envolvimento de nenhum outro sistema, classificou-se como linfoma primário de bexiga extranodal. O animal passou pelo tratamento quimioterápico realizando nove sessões de quimioterapia pelo protocolo de CHOP, contudo devido ao agravamento do caso a paciente veio a óbito cerca de sete meses após o diagnóstico da doença. O caso estudado foi de extrema importância para a compreensão de linfomas primários de bexiga em razão da escassez de informações relacionadas na literatura. Ainda, o cão é um excelente modelo experimental de linfomas não Hodgking em humanos, consequentemente compreender essa doença em cães promove a evolução conjunta da medicina humana.

Lymphoma is a highly recurrent rate neoplasm in the oncology routine of veterinary medicine. It can be classified into Hodgking-like, rarely described just in felines, and non-Hodgking lymphoma, the latter being the most commun, subdivided into B-cell lymphoma and T-cell lymphoma. The objective of this study was to report the clinical and therapeutic conduct within the diagnosis procedures of a 12-years-old female dog, mixed breed, who manifested dysuria, prostation, abdominal pain and on the physical examination a mass in the hypogastric region was noticed. This was diagnosed as a large cell lymphoma by means cytology and biopsy, also immunohistochemistry was required which confirmed the diffuse large cell lymphoma of immunophenotyping B. Without any other sistem envolved, the neoplasm was classified as primary urinary bladder lymphoma extranodal. The animal underwent chemotherapy, performing nine sessions according to the Madison protocol, however, due to the worsening of the case, the patient died about seven months after the diagnosis of the disease. This case was extremely importante for the understanding of primary urinary bladder lymphomas due to the scarcity of informations in the literature. Also, dog is an excellent experi,emtal model of non Hodgking lymphomas in humans, thus understandig this disease in dogs promotes the joint evolution of human medicine.

Parasitological and immunological evaluation of cattle experimentally infected with Trypanosoma vivax.

Trypanosoma vivax infection causes relevant economical impact due to high morbidity and mortality leading to negative impact on local livestock. Despite parasitological and serological methods are used for the diagnosis of T. vivax infection, gaps regarding sensitivity and specificity of these methods still represent a challenge. The present study aimed to compare the kinetics of parasitological and serological parameters in cattle experimentally infected with T. vivax along with immunophenotypic analysis of whole blood leukocytes. Based on the parasitemia profile the analysis were performed in three distinct periods, referred as pre-patent, patent and post-treatment. Distinct kinetics of anti-T. vivax IgM and IgG were observed during the pre-patent, patent and post-treatment periods. Increased levels of WC1+ γδ T-cells were observed throughout the infection with strong correlations with other biomarkers observed during post-treatment period. Our findings demonstrated that there is a important participation of Monocytes:CD14+; NK-cells:CD335+ and WC1+ γδ T-cells that coincide with the peak of parasitemia and also with the adaptive immunity, specially CD4+ T-cells in T. vivax infection. The knowledge of the immune response is important not only for understanding the biology of the parasite in the host, but for the design of new treatment strategies for trypanosome infections.

Prevalência de parasitos gastrintestinais e de toxoplasmose em ovinos da região de Uberaba, MG / Prevalence of gastrointestinal parasites and toxoplasmosis sheep in the region of Uberaba, state of Minas Gerais

Com o objetivo de identificar os principais parasitos gastrintestinais que acometem os ovinos, bem como a ocorrência de anticorpos IgG contra Toxoplasma gondii, foram realizados 270 exames coprológicos (OPG/OOPG) e 250 sorológicos (imunofluorescência indireta-IFI) nos ovinos da região de Uberaba-MG. Das amostras de fezes coletadas, 217 (80,37%) eram positivas, com prevalência de Strongyloidea (88,47%), Eimeria spp. (63,13%), Strongyloides sp. (24,42%), Skrjabinema sp. (0,73%) e Moniezia sp. (0,73%) e Capillaria sp. (0,46%). Dos 250 soros testados para Toxoplasma gondii, 144 (57,60%) apresentavam títulos acima do ponto de corte (64), sendo 77,78% com título de 64 e 22,22% de 128. Esses resultados são importantes para nortear proprietários e veterinários sobre a necessidade de diagnosticar e controlar as parasitoses que acometem os rebanhos ovinos.

In order to identify the gastrointestinal sheep´s parasites, and the occurrence of antibodies against Toxoplasma gondii, 270 stool tests (OPG/OOPG) and 250 serological tests (indirect immunofluorescence (IIF) were performed in properties in the region of Uberaba-MG. From the stool samples collected 217 (80.37%) were positive, with a prevalence of Strongyloidea (88.47%), Eimeria spp. (63.13%), Strongyloides sp. (24.42%), Skrjabinema sp. (0.73%), Moniezia (0.73%) and 0.46% Capillaria sp. From the 250 sera tested for Toxoplasma gondii, 144 (57.60%) had titers above the cut-off (64), 77.77% with title 64 and 22.22% with title 128. These results are important to guide owners and veterinarians about the need for prevention and control methods for parasites that affect sheep flocks.