Nitric Oxide Detection Using a Corona Phase Molecular Recognition Site on Chiral Single-Walled Carbon Nanotubes.

Semiconducting single-walled carbon nanotubes (s-SWCNT) are structures that fluoresce in the near-infrared region. By coating SWCNT surfaces with polymeric materials such as single-chain DNA, changes in fluorescence emission occur in the presence of reagents. In this way, polymer-coated SWCNT structures allow them to be used as optical sensors for single molecule detection. Especially today, the inadequacy of the methods used in the detection of cellular molecules makes the early diagnosis of diseases such as cancer difficult at the single-molecule level. In this study, the detection of nitric oxide (NO) signals, which are a marker of cancer, was carried out at the single-molecule level. In this context, a sensor structure was formed by coating the 7,6-chiral s-SWCNT surface with ssDNA with different oligonucleotide lengths (AT). The sensor structure was characterized by using UV-vis spectroscopy and Raman spectroscopy microscopy. After formation of the sensor structure, a selectivity library was created using various molecules. As a result of the coating of the SWCNT (7,6) surface with DNA corona phase formation, Raman peaks at 195 and 276 cm-1 were observed to shift to the right. Additionally, the selectivity library results showed that the (AT)30 sequence can be used in NO detection. As a result of the studies using SWCNT (7.6)- (AT)30, the limit of detection (LOD) and limit of determination (LOQ) values of the sensor against NO were found to be 1.24 and 4.13 µM, respectively.

Photocatalytic activity of TiO2-ZnO/g-C3N4 nanocomposites for methylene orange and Rhodamine B dyes removal from water and photocatalytic hydrogen generation.

In this work, for the removal of azo dyes that cause environmental pollution, TiO2-ZnO has been modified with graphitic carbon nitride (g-C3N4) to form an advanced hetero-linked photocatalyst. With this catalyst, photocatalytic hydrogen production and photodegradation activity against methylene orange (MO) and rhodamineB (RhB) dye removal were studied. The synthesized nanostructure was extensively characterized by several techniques such as XRD, TEM, UV-Vis and fluorescence spectrophotometer (PL) techniques. According to the analysis, a significant increase in the photocatalytic efficiency of TiO2-ZnO was determined after it was modified with g-C3N4 nanostructures. The combination between TiO2-ZnO and g-C3N4 was shown to be responsible for the improvement in photocatalytic activity because it significantly decreased electron-hole recombination. After 90 min the 62.81% of MO dye was removed but at 120 min only 57% of RhB was degraded. In addition, the antibacterial activity of TiO2-ZnO/g-C3N4 catalyst was carried out against gram positive and gram negatif bacteria. The bacterial inhibition (%) of TiO2-ZnO/g-C3N4 catalyst.was found to be 44 % against E. coli and 33 % against at 100 µg/ml concentration. In line with the analyzes obtained with this study, important results have been revealed for the application of photocatalytic methods in more industrial dimensions in the production of hydrogen, which is a valuable energy type.

Bimetallic Biogenic Pt-Ag Nanoparticle and Their Application for Electrochemical Dopamine Sensor.

In this study, Silver-Platinum (Pt-Ag) bimetallic nanoparticles were synthesized by the biogenic reduction method using plant extracts. This reduction method offers a highly innovative model for obtaining nanostructures using fewer chemicals. According to this method, a structure with an ideal size of 2.31 nm was obtained according to the Transmission Electron Microscopy (TEM) result. The Pt-Ag bimetallic nanoparticles were characterized using Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffractometry (XRD), and Ultraviolet-Visible (UV-VIS) spectroscopy. For the electrochemical activity of the obtained nanoparticles in the dopamine sensor, electrochemical measurements were made with the Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV) methods. According to the results of the CV measurements taken, the limit of detection (LOD) was 0.03 µM and the limit of quantification (LOQ) was 0.11 µM. To investigate the antibacterial properties of the obtained Pt-Ag NPs, their antibacterial effects on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria were investigated. In this study, it was observed that Pt-Ag NPs, which were successfully synthesized by biogenic synthesis using plant extract, exhibited high electrocatalytic performance and good antibacterial properties in the determination of dopamine (DA).

In situ preparation of TiO2/f-MWCNT catalyst using Pluronic F127 assisted sol-gel process for sonocatalytic degradation of methylene blue.

In this study, titanium dioxide- Pluronics @F127/functionalized -multi walled carbon nanotubes (TiO2-F127f-/MWCNT) nanocatalysts were prepared, characterized, and used in methylene blue (MB) degradation under ultrasonic conditions. The characterization studies were performed using TEM, SEM, and XRD analyses to reveal the morphological and chemical properties of TiO2-F127/MWCNT nanocatalysts. To detect the optimum parameters for MB degradation using TiO2-F127/f-MWCNT nanocatalysts, several experimental parameters were conducted at various conditions such as different temperatures, pH, catalyst amount, hydrogen peroxide (H2O2) concentration, and various reaction contents. Transmission electron microscopy (TEM) analyses showed that TiO2-F127/f-MWCNT nanocatalysts consisted of a homogenous structure and have a 12.23 nm particle size. The crystalline particle size of TiO2-F127/MWCNT nanocatalysts was found to be 13.31 nm. Scanning electron microscope (SEM) analyses revealed the surface structure of TiO2-F127/f-MWCNT nanocatalysts turned to be modified after TiO2 loaded on MWCNT. Under the optimum conditions; pH: 4, MB concentration: 25 mg/L, H2O2 concentration: 30 mol/L, reaction time: and catalyst dose: 24 mg/L, chemical oxygen demand (COD) removal efficiency reached a maximum of 92%. To detect the radical effectiveness, three scavenger solvents were tested. Reuse experiments revealed that TiO2-F127/f-MWCNT nanocatalysts retained 84.2% catalytical activity after 5 cycles. Gas chromatography-mass spectrometry (GC-MS) was successfully used to identify the generated intermediates. Based on the experimental results, it has been suggested that •OH radicals are the main active species responsible for the degradation reaction in the presence of the TiO2-F127/f-MWCNT nanocatalysts.

Modification of multi-walled carbon nanotubes with platinum-osmium to develop stable catalysts for direct methanol fuel cells.

In the study, a new bimetallic catalyst was synthesized for methanol oxidation using multi-walled carbon nanotube (MWCNT)-supported platinum-osmium (PtOs) nanoparticles (PtOs@MWCNT NPs). The morphological structures of the prepared NPs were examined using different techniques, such as scanning electron microscopy (SEM) and X-ray diffraction (XRD). The electrochemical characterization of the synthesized PtOs@MWCNT catalysts, such as chronoamperometry (CA), cyclic voltammetry (CV), scan rate (SR) analysis, cyclic catalytic test, and electrochemical surface area (ECSA) evaluation, were performed in an alkaline medium. From the results obtained, the size of the NPs was found to be 3.12 nm according to the Debye-Schrrer equation, and the MWCNTs were clearly observed by SEM imaging. After the characterization of the prepared nanomaterials, the PtOs@MWCNT catalysts were employed in the methanol oxidation reaction, and a high oxidation current value of 220.86 mA cm-2 was observed. Besides, according to the CA results, the catalyst exhibited high stability for 4000 s, and it was seen that Os metal improved the catalytic activity of the main catalyst. These results show that the PtOs@MWCNT catalyst is highly stable and reusable, and provides high electrocatalytic activity in the methanol oxidation reaction. Moreover, the obtained catalyst gave ideal results in terms of CO tolerance and activity. These data show that the obtained catalyst will provide significant improvement and superior efficiency in fuel-cell applications.

Synthesis and characterization of activated carbon supported bimetallic Pd based nanoparticles and their sensor and antibacterial investigation.

Activated carbon (AC) supported palladium cobalt bimetallic nanoparticles (PdCo@AC NPs) were obtained by green synthesis method using Cinnamomum verum (C. Verum) extract. The obtained NPs were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Crystallography (XRD), Transmission Electron Microscope (TEM) and Ultraviolet Visible (UV-VIS) spectroscopy, and the functional groups and morphology of the nanoparticle were elucidated. The resulting particle size was found to be 2.467 nm. NPs were evaluated using Cyclic Voltammetry (CV), Scan Rate (SR), and Differential Pulse Voltammetry (DPV) techniques for potential dopamine sensors application. According to the obtained DPV results, Limit of Detection (LOD) and Limit of Quantitation (LOQ) values are found to be 5.68 pM and 17.21 pM, respectively. It was also observed that AC supported PdCo nanoparticles obtained from C. verum extract sensed dopamine quite well. Besides, to examine the antibacterial properties of NPs, antibacterial analyzes were performed with Escherichia coli (E. Coli) and Staphylococcus aureus (S. Aureus). It was observed that it showed good antibacterial properties against gram positive (S. aureus) and gram negative (E. coli) bacteria. The study gave important results in terms of the synthesis of bimetallic NPs using the green synthesis method and their usability in different areas. With this study, it was observed that a good antibacterial dopamine sensor were obtained with the successful biogenic synthesis of AC supported PdCo bimetallic NPs.

The removal of heavy metal pollution from wastewaters using thermophilic B. cereus SO-16 bacteria.

In this study, bioaccumulation, remediation, tolerance, and effects of manganese ions (Mn(II)) and copper ions (Cu(II)) on antioxidant enzymes of thermophilic Bacillus cereus (B. cereus) SO-16 were investigated in detail. The findings of the study showed that Mn(II) was less toxic than Cu(II) to B. cereus SO-16. Moreover, B. cereus SO-16 was exhibited less tolerance to Mn(II) and Cu(II) ions in the liquid medium compared to the solid medium. The growth of bacteria was expressively effective for Mn(II) and Cu(II) concentrations of 2.5 mg/L at 24th h. The highest Mn(II) and Cu(II) bioaccumulation values after 48 h incubation of thermophilic B. cereus SO-16 were measured as 102.04 (24th h) and 87.96 (36th h) metal/dry bacteria weight. The change in morphology and functionality of B. cereus SO-16 after interaction with Mn(II) and Cu(II) was tested using various methods. The results indicated that B. cereus SO-16, a thermophilic bacterium, can be utilized in industrial wastewaters to recover and remediation of toxic metals.

Synthesis and application of AuNi@AC nano adsorbents for the removal of Maxilon Blue 5G azo dye from aquatic mediums.

In this research, gold-nicel supported on activated carbon (AC) nanoadsorbent (AuNi@AC) synthesized by following a series of physicochemical procedures was prepared for the removal of Maxilon Blue 5G (MB) which is a cationic textile dye. Experimental studies based on parameters specifically pH, contact time, nano catalytic adsorbent particle, initial MB dye concentration and temperature effect were conducted in aqueous solutions in a batch system. AuNi@AC nanoadsorbents (NAs) reached the equilibrium in 30 min under optimum conditions in adsorption of the dye. The pseudo-first, second-order, and intra-particle diffusion models were tested to evaluate a the experimental results. Adsorption kinetics were found to be represented by the pseudo-second-order model, and the maximum adsorption capacity (qmax.) was calculated to be 542.90 mg/g (or 2.041 mmol/g). The synthesized magnetic AuNi@AC nanoadsorbent showed a high-efficiency reusability effect of about 64% after five reuse runs. Also, thermodynamic function parameters such as activation energy (Ea), Gibbs free energy (ΔG *), and entropy (ΔS *) were investigated in the sorption study. After all evaluation of data, it was concluded that the novel AuNi@AC nanoadsorbent could be considered as an effective support material for the removal of various organic pollutants in aquation solution especially for the removal of MB.

Fabrication of activated carbon supported modified with bimetallic-platin ruthenium nano sorbent for removal of azo dye from aqueous media using enhanced ultrasonic wave.

Herein, activated carbon supported modified with bimetallic-platin ruthenium nano sorbent (PtRu@AC) was synthesized by a thermal decomposition process and used in the removal of methylene blue (MB) from aqueous solutions. The synthesized nano sorbents were characterized by X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), and X-ray Photoelectron Spectroscopy (XPS) spectroscopic techniques. The data obtained from characterization studies showed that PtRu@AC nano sorbent was highly crystalline and in a form of PtRu alloy with a monodispersed composition. The results indicated that the maximum adsorption capacity (qemax) for the removal of MB with PtRu@AC under optimum conditions was detected to be 1.788 mmol/g (569.4 mg/g). The experimental kinetic results of the study revealed that the adsorption of methylene blue was found to be more compatible with the false second-order model compared to some tested models. Calculations for thermodynamic functions including enthalpy change (ΔHo), entropy change (ΔSo), and Gibbs free energy change (ΔGo) values were performed to get an idea about the adsorption mechanism. As a result, the synthesized PtRu@AC nano adsorbent was detected as a highly effective adsorbent material in the removal of MB from aquatic mediums.

Preconcentrations of Cu (II) and Mn (II) by magnetic solid-phase extraction on Bacillus cereus loaded γ-Fe2O3 nanomaterials.

For the simultaneous preconcentrations of Cu(II) and Mn(II), a novel preconcentration technique was developed and described. Bacillus cereus loaded magnetic É£-Fe2O3 nanoparticles were prepared and used as support materials on solid-phase extraction procedure. Important experimental parameters were investigated in details and pH 6.0, 3 mL min-1 of flow rate, 5 mL of 1 mol L-1 of HCl as eluent, 200 mg of biomass, and 200 mg of magnetic É£-Fe2O3 nanoparticles as support material was found as the best conditions. The preconcentrations factor were found to be 80 for Cu (II) and Mn(II). It was confirmed by the results that SPE columns could be used in 32 cycles. The LOD values calculated for Cu (II) and Mn (II) were 0.09 and 0.08 ng mL-1, respectively. The RSD values found were less than 3.4%. The extraction recoveries were achieved as higher than 98%. The biosorption capacities of Cu (II), and Mn (II) were 26.0 mg g-1, 30.3 mg g-1 respectively. The approach devised for analyzing analyte concentrations in food samples proved to be successful.

Investigation of antibacterial, antifungal, antibiofilm, antioxidant and anticancer properties of methanol extracts of Salvia marashica Ilçim, Celep & Dogan and Salvia caespitosa Montbret & Aucher ex Benth plants with medicinal importance.

In this study, Antimicrobial, Antifungal and Antibiofilm activity tests on 16 bacteria and 2 fungi of Salvia marashica Ilçim, Celep & Dogan and Salvia caespitosa Montbret & Aucher ex Benth species were performed by Agar Well Diffusion and Microdilution methods. Salvia species showed sensitivity with a zone diameter of 12-16 mm. Concentrations of 0.5 mg/ml to 16 mg/ml by the dilution method were used to determine the Minimum inhibited concentration (MIC) and Minimum bactericidal concentration (MBK). The MIC values of the plants are mostly 0.5 mg/ml - 4 mg/ml and MBC values are between 0.5 mg/ml - 8 mg/ml. Antifungal activity findings are remarkable on the species and it has been observed to have very high effects especially on Candida parapsilosis. When the antioxidant research findings are examined; It was observed that Salvia marashica and Salvia caespitosa plants have approximately 75% antioxidant activity at 1 mg/ml, and findings mostly directly proportional between concentrations and antioxidant activity capacity were recorded. MCF-7 and HUVEC cell lines were used to investigate anticancer activity properties. In line with the findings, while the IC50 value of Salvia marashica on the MCF-7 Cell line was 0.125 mg/ml, it was 1.65 mg/ml in the HUVEC cell line, while the IC50 value of Salvia caespitosa on the MCF-7 Cell line was 0.115 mg/ml in the HUVEC Cell line. It was found to be 9.87 mg/ml. It has been proven that both Salvia species have a cytotoxic effect on the MCF-7 Cell line.

Glucose nano biosensor with non-enzymatic excellent sensitivity prepared with nickel-cobalt nanocomposites on f-MWCNT.

NiCo (Nickel-cobalt) nanoparticles were obtained by the chemical reduction method on functionalized multi-walled carbon nanotubes. After this process, chronoamperometry, cyclic voltammetry, and amperometric methods were used to investigate the electrochemical and electrocatalytic behavior of NiCo@f-MWCNT against glucose oxidation. In addition, the NiCo@f-MWCNT nanocomposites were analyzed by characterization techniques such as X-Ray Diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Transmission Electron Microscopy (TEM), and Atomic Force Microscopy (AFM) in terms of the morphological and atomic structure of prepared nanomaterials. The sensitivity and limit of detection the non-enzymatic glucose sensor (NiCo@f-MWCNT) were calculated as 10,015 µA/mM-1 cm-2 0.26 µM, respectively. As a result of these studies and experiments, the NiCo@f-MWCNT nanocomposite is a really good sensor and their stability showed that the current nanomaterials expressed to be new material for the electrochemical detection of glucose.

Retraction Note: Suppressor capacity of copper nanoparticles biosynthesized using Crocus sativus L. leaf aqueous extract on methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line.

Editor's Note: this Article has been retracted; the Retraction Note is available at https://doi.org/10.1038/s41598-020-77741-4 .

Suppressor capacity of copper nanoparticles biosynthesized using Crocus sativus L. leaf aqueous extract on methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line.

In this research, we prepared and formulated a neuroprotective supplement (copper nanoparticles in aqueous medium utilizing Crocus sativus L. Leaf aqueous extract) for determining its potential against methadone-induced cell death in PC12. The results of chemical characterization tests i.e., FE-SEM, FT-IR, XRD, EDX, TEM, and UV-Vis spectroscopy revealed that the study showed that copper nanoparticles were synthesized in the perfect way possible. In the TEM and FE-SEM images, the copper nanoparticles were in the mean size of 27.5 nm with the spherical shape. In the biological part of the present research, the Rat inflammatory cytokine assay kit was used to measure the concentrations of inflammatory cytokines. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) test was used to show DNA fragmentation and apoptosis. Caspase-3 activity was assessed by the caspase activity colorimetric assay kit and mitochondrial membrane potential was studied by Rhodamine123 fluorescence dye. Also, the cell viability of PC12 was measured by trypan blue assay. Copper nanoparticles-treated cell cutlers significantly (p ≤ 0.01) decreased the inflammatory cytokines concentrations, caspase-3 activity, and DNA fragmentation and they raised the cell viability and mitochondrial membrane potential in the high concentration of methadone-treated PC12 cells. The best result of neuroprotective properties was seen in the high dose of copper nanoparticles i.e., 4 µg. According to the above results, copper nanoparticles containing C. sativus leaf aqueous extract can be used in peripheral nervous system treatment as a neuroprotective promoter and central nervous system after approving in the clinical trial studies in humans.