RESUMO
BACKGROUND: Birth weight and sodium intake are both associated with risk for hypertension. It is not known whether birth weight influences response to salty taste. OBJECTIVE: To assess the relationship between birth weight and salty taste acceptance of infants and young children. DESIGN: Acceptance of salty taste was assessed at 2 (n = 80) and 6 (n = 76) months in infants (birth weight >2.5 kg) enrolled in a prospective cohort study. Acceptance was expressed as proportional intake following 1-min ingestion tests with water and salt solutions (0.17 and 0.34 mol/l NaCl, in water). Birth weight was obtained by maternal report. Questionnaires completed by mothers and food-ranking procedures performed by children evaluated salt liking and preference in a subset (n = 38) of subjects at preschool age (36 or 48 months). SETTING: Nonprofit basic research institute in Philadelphia, PA, USA. RESULTS: Regression analysis revealed significant negative associations between birth weight and acceptance of salty taste at 2 months (0.17 mol/l, P < 0.0001; 0.34 mol/l, P < 0.01) but not at 6 months. Relationships were not affected by adjustment for potential confounders. In preschoolers, greater liking of (P < 0.05) and preference for (P < 0.01) salty foods was associated with lower birth weight in simple, but not adjusted, models. CONCLUSION: Measures related to salty taste preference were inversely related to birth weight over the first 4 years of life. Additional studies should substantiate these findings and explore whether early response to salty taste predicts future sodium intake, blood pressure, or other public health-related outcomes. SPONSORSHIP: National Institutes of Health (DC 00882).
Assuntos
Peso ao Nascer/fisiologia , Preferências Alimentares/fisiologia , Cloreto de Sódio , Paladar/fisiologia , Pré-Escolar , Estudos de Coortes , Feminino , Humanos , Lactente , Recém-Nascido , Estudos Longitudinais , Masculino , Estudos Prospectivos , Inquéritos e QuestionáriosRESUMO
The results of recent studies suggest that the mouse Sac (saccharin preference) locus is identical to the Tas1r3 (taste receptor) gene. The goal of this study was to identify Tas1r3 sequence variants associated with saccharin preference in a large number of inbred mouse strains. Initially, we sequenced approximately 6.7 kb of the Tas1r3 gene and its flanking regions from six inbred mouse strains with high and low saccharin preference, including the strains in which the Sac alleles were described originally (C57BL/6J, Sac(b); DBA/2J, Sac(d)). Of the 89 sequence variants detected among these six strains, eight polymorphic sites were significantly associated with preferences for 1.6 mm saccharin. Next, each of these eight variant sites were genotyped in 24 additional mouse strains. Analysis of the genotype-phenotype associations in all 30 strains showed the strongest association with saccharin preference at three sites: nucleotide (nt) -791 (3 bp insertion/deletion), nt +135 (Ser45Ser), and nt +179 (Ile60Thr). We measured Tas1r3 gene expression, transcript size, and T1R3 immunoreactivity in the taste tissue of two inbred mouse strains with different Tas1r3 haplotypes and saccharin preferences. The results of these experiments suggest that the polymorphisms associated with saccharin preference do not act by blocking gene expression, changing alternative splicing, or interfering with protein translation in taste tissue. The amino acid substitution (Ile60Thr) may influence the ability of the protein to form dimers or bind sweeteners. Here, we present data for future studies directed to experimentally confirm the function of these polymorphisms and highlight some of the difficulties of identifying specific DNA sequence variants that underlie quantitative trait loci.
Assuntos
Comportamento de Escolha/fisiologia , Preferências Alimentares/fisiologia , Polimorfismo Genético , Receptores de Superfície Celular/genética , Sacarina/farmacologia , Paladar/genética , Animais , Comportamento de Escolha/efeitos dos fármacos , Preferências Alimentares/efeitos dos fármacos , Expressão Gênica , Frequência do Gene , Genótipo , Masculino , Camundongos , Camundongos Endogâmicos , Receptores de Superfície Celular/biossíntese , Receptores Acoplados a Proteínas G , Especificidade da Espécie , Paladar/efeitos dos fármacos , Paladar/fisiologia , Papilas Gustativas/metabolismoRESUMO
Odours play a critical role in the behaviour and physiology of many species. For mice and probably many other species, including humans, an individual's olfactory identity (its odourtype) is coded in part by a pattern of volatile compounds that is regulated by genes in the major histocompatibility complex, a string of linked genes that is intimately involved in immune function. The mouse olfactory system is exquisitely sensitive to minute variations in odourtypes. Layered within these chemical signals of individuality is information on the age and health status of the mouse. In the case of age, it appears that information is coded based on a pattern of volatile metabolites; we do not know how a mouse detects, for example, the presence of a viral infection in volatiles from an infected mouse. This chemical information serves to regulate mate choice and other aspects of social behaviour.
Assuntos
Odorantes , Transdução de Sinais , Envelhecimento , Animais , Comportamento de Escolha , Infecções/metabolismo , Complexo Principal de Histocompatibilidade , Camundongos , Neurônios Receptores Olfatórios/metabolismo , Olfato , Comportamento SocialRESUMO
An acetylated sugar, sucrose octaacetate (SOA), tastes bitter to humans and has an aversive taste to at least some mice and other animals. In mice, taste aversion to SOA depends on allelic variation of a single locus, Soa. Three Soa alleles determine 'taster' (Soa(a)), 'nontaster' (Soa(b)), and 'demitaster' (Soa(c)) phenotypes of taste sensitivity to SOA. Although Soa has been mapped to distal Chromosome (Chr) 6, the limits of the Soa region have not been defined. In this study, mice from congenic strains SW.B6-Soa(b), B6.SW-Soa(a), and C3.SW-Soa(a/c) and from an outbred CFW strain were genotyped with polymorphic markers on Chr 6. In the congenic strains, the limits of introgressed donor fragments were determined. In the outbred mice, linkage disequilibrium and haplotype analyses were conducted. Positions of the markers were further resolved by using radiation hybrid mapping. The results show that the Soa locus is contained in an approximately 1-cM (3.3-4.9 Mb) region including the Prp locus.
Assuntos
Cromossomos , Camundongos Endogâmicos/genética , Sacarose/análogos & derivados , Sacarose/metabolismo , Paladar/genética , Animais , Animais não Endogâmicos , Mapeamento Cromossômico/métodos , Cricetinae , Feminino , Genótipo , Desequilíbrio de Ligação , Masculino , Camundongos , Camundongos Endogâmicos C3H/genética , Camundongos Endogâmicos C57BL , Fenótipo , Limiar Gustativo/fisiologiaRESUMO
Previous studies have shown large differences in taste responses to several sweeteners between mice of the C57BL/6ByJ (B6) and 129P3/J (129) inbred strains. The goal of this study was to compare behavioral responses of B6 and 129 mice to a wider variety of sweeteners. Seventeen sweeteners were tested using two-bottle preference tests with water. Three main patterns of strain differences were evident. First, sucrose, maltose, saccharin, acesulfame-K, sucralose and SC-45647 were preferred by both strains, but the B6 mice had lower preference thresholds and higher solution intakes. Second, the amino acids D-phenylalanine, D-tryptophan, L-proline and glycine were highly preferred by B6 mice, but not by 129 mice. Third, glycyrrhizic acid, neohesperidin dihydrochalcone, thaumatin and cyclamate did not evoke strong preferences in either strain. Aspartame was neutral to all 129 and some B6 mice, but other B6 mice strongly preferred it. Thus, compared with the 129 mice the B6 mice had higher preferences for sugars, sweet tasting amino acids and several but not all non-caloric sweeteners. Glycyrrhizic acid, neohesperidin, thaumatin and cyclamate are not palatable to B6 or 129 mice.
Assuntos
Sacarose/análogos & derivados , Limiar Gustativo , Paladar/efeitos dos fármacos , Animais , Relação Dose-Resposta a Droga , Preferências Alimentares/efeitos dos fármacos , Masculino , Maltose/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Sacarina/farmacologia , Soluções/farmacologia , Especificidade da Espécie , Sacarose/farmacologia , Edulcorantes/farmacologia , Tiazinas/farmacologiaRESUMO
The C57BL/6ByJ (B6) strain of mice exhibits higher preferences than does the 129P3/J (129) strain for a variety of sweet tasting compounds. We measured gustatory afferent responses of the whole chorda tympani nerve in these two strains using a broad array of sweeteners and other taste stimuli. Neural responses were greater in B6 than in 129 mice to the sugars sucrose and maltose, the polyol D-sorbitol and the non-caloric sweeteners Na saccharin, acesulfame-K, SC-45647 and sucralose. Lower neural response thresholds were also observed in the B6 strain for most of these stimuli. The strains did not differ in their neural responses to amino acids that are thought to taste sweet to mice, with the exception of L-proline, which evoked larger responses in the B6 strain. Aspartame and thaumatin, which taste sweet to humans but are not strongly preferred by B6 or 129 mice, did not evoke neural responses that exceeded threshold in either strain. The strains generally did not differ in their neural responses to NaCl, quinine and HCl. Thus, variation between the B6 and 129 strains in the peripheral gustatory system may contribute to differences in their consumption of many sweeteners.
Assuntos
Nervo da Corda do Tímpano/efeitos dos fármacos , Sacarose/análogos & derivados , Edulcorantes/farmacologia , Limiar Gustativo , Animais , Aspartame/farmacologia , Relação Dose-Resposta a Droga , Eletrofisiologia , Glucanos/farmacologia , Guanidinas/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Oligossacarídeos/farmacologia , Proteínas de Plantas/farmacologia , Quinina/farmacologia , Sacarina/farmacologia , Especificidade da Espécie , Sacarose/farmacologia , Paladar/efeitos dos fármacos , Tiazinas/farmacologiaRESUMO
Differences in sweetener intake among inbred strains of mice are partially determined by allelic variation of the saccharin preference (Sac) locus. Genetic and physical mapping limited a critical genomic interval containing Sac to a 194 kb DNA fragment. Sequencing and annotation of this region identified a gene (Tas1r3) encoding the third member of the T1R family of putative taste receptors, T1R3. Introgression by serial backcrossing of the 194 kb chromosomal fragment containing the Tas1r3 allele from the high-sweetener-preferring C57BL/6ByJ strain onto the genetic background of the low-sweetener-preferring 129P3/J strain rescued its low-sweetener-preference phenotype. Polymorphisms of Tas1r3 that are likely to have functional significance were identified using analysis of genomic sequences and sweetener-preference phenotypes of genealogically distant mouse strains. Tas1r3 has two common haplotypes, consisting of six single nucleotide polymorphisms: one haplotype was found in mouse strains with elevated sweetener preference and the other in strains relatively indifferent to sweeteners. This study provides compelling evidence that Tas1r3 is equivalent to the Sac locus and that the T1R3 receptor responds to sweeteners.
Assuntos
Clonagem Molecular , Preferências Alimentares , Sacarina/farmacologia , Alelos , Sequência de Aminoácidos , Animais , Mapeamento Cromossômico , Mapeamento de Sequências Contíguas , Cruzamentos Genéticos , Feminino , Biblioteca Gênica , Ligação Genética , Variação Genética , Genótipo , Haplótipos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Modelos Genéticos , Dados de Sequência Molecular , Fenótipo , Mapeamento Físico do Cromossomo , Polimorfismo Genético , Polimorfismo de Nucleotídeo Único , Homologia de Sequência de Aminoácidos , Limiar GustativoRESUMO
BACKGROUND: Flavors from the mother's diet during pregnancy are transmitted to amniotic fluid and swallowed by the fetus. Consequently, the types of food eaten by women during pregnancy and, hence, the flavor principles of their culture may be experienced by the infants before their first exposure to solid foods. Some of these same flavors will later be experienced by infants in breast milk, a liquid that, like amniotic fluid, comprises flavors that directly reflect the foods, spices, and beverages eaten by the mother. The present study tested the hypothesis that experience with a flavor in amniotic fluid or breast milk modifies the infants' acceptance and enjoyment of similarly flavored foods at weaning. METHODS: Pregnant women who planned on breastfeeding their infants were randomly assigned to 1 of 3 groups. The women consumed either 300 mL of carrot juice or water for 4 days per week for 3 consecutive weeks during the last trimester of pregnancy and then again during the first 2 months of lactation. The mothers in 1 group drank carrot juice during pregnancy and water during lactation; mothers in a second group drank water during pregnancy and carrot juice during lactation, whereas those in the control group drank water during both pregnancy and lactation. Approximately 4 weeks after the mothers began complementing their infants' diet with cereal and before the infants had ever been fed foods or juices containing the flavor of carrots, the infants were videotaped as they fed, in counterbalanced order, cereal prepared with water during 1 test session and cereal prepared with carrot juice during another. Immediately after each session, the mothers rated their infants' enjoyment of the food on a 9-point scale. RESULTS: The results demonstrated that the infants who had exposure to the flavor of carrots in either amniotic fluid or breast milk behaved differently in response to that flavor in a food base than did nonexposed control infants. Specifically, previously exposed infants exhibited fewer negative facial expressions while feeding the carrot-flavored cereal compared with the plain cereal, whereas control infants whose mothers drank water during pregnancy and lactation exhibited no such difference. Moreover, those infants who were exposed to carrots prenatally were perceived by their mothers as enjoying the carrot-flavored cereal more compared with the plain cereal. Although these same tendencies were observed for the amount of cereal consumed and the length of the feeds, these findings were not statistically significant. CONCLUSIONS: Prenatal and early postnatal exposure to a flavor enhanced the infants' enjoyment of that flavor in solid foods during weaning. These very early flavor experiences may provide the foundation for cultural and ethnic differences in cuisine.
Assuntos
Feto/fisiologia , Preferências Alimentares/fisiologia , Recém-Nascido/fisiologia , Aprendizagem/fisiologia , Troca Materno-Fetal/fisiologia , Leite Humano/química , Paladar/fisiologia , Líquido Amniótico/química , Líquido Amniótico/fisiologia , Bebidas , Daucus carota/química , Dieta/métodos , Expressão Facial , Feminino , Humanos , Leite Humano/fisiologia , Gravidez , DesmameRESUMO
In mice, behavioral acceptance of the bitter compound sucrose octaacetate (SOA) depends on allelic variation of a single gene, Soa. The SW.B6-Soa(b)congenic mouse strain has the genetic background of an "SOA taster" SWR/J strain and an Soa-containing donor chromosome fragment from an "SOA nontaster" C57BL/6J strain. Using microsatellite markers polymorphic between the two parental strains, we determined that the donor fragment spans 5-10 cM of distal chromosome 6. The SWR/J mice avoided SOA in two-bottle tests with water and had strong responses to SOA in two gustatory nerves, the chorda tympani (CT) and glossopharyngeal (GL). In contrast, the SW.B6-Soa(b) mice were indifferent to SOA in two-bottle tests and had very weak responses to SOA in both of these nerves. The SWR/J and SW.B6-Soa(b) mice did not differ in responses of either nerve to sucrose, NaCl, HCl, or the bitter-tasting stimuli quinine, denatonium, strychnine, 6-n-propylthiouracil, phenylthiocarbamide, and MgSO(4). Thus the effect of the Soa genotype on SOA avoidance is mediated by peripheral taste responsiveness to SOA, involving taste receptor cells innervated by both the CT and GL nerves.
Assuntos
Proteínas de Drosophila , Proteínas de Membrana/genética , Receptores de Superfície Celular/genética , Sacarose/farmacologia , Paladar/genética , Paladar/fisiologia , Língua/inervação , Animais , Nervo da Corda do Tímpano/efeitos dos fármacos , Nervo da Corda do Tímpano/fisiologia , Mapeamento Cromossômico , Eletrofisiologia , Genótipo , Nervo Glossofaríngeo/efeitos dos fármacos , Nervo Glossofaríngeo/fisiologia , Masculino , Camundongos , Camundongos Congênicos , Sacarose/análogos & derivadosRESUMO
Purified carbohydrates and fats are usually palatable to humans and other animals, and their consumption often induces weight gain and accumulation of fat. In this study, we examined consumption of complex carbohydrates (cornstarch and Polycose) and fats (soybean oil and margarine) in mice from two inbred strains, C57BL/6ByJ and 129P3/J. At lower concentrations of liquid nutrients tested using two-bottle tests, when the amounts consumed had negligible energy content, the C57BL/6ByJ mice had higher acceptance of Polycose and soybean oil. This was probably due to strain differences in chemosensory perception of Polycose and oil. At higher concentrations, the mice consumed a substantial part of their daily energy from the macronutrient sources, however, there were no or only small strain differences in nutrient consumption. These small differences were probably due to strain variation in body size. The two strains also did not differ in chow intake. Despite similar energy intakes, access to the nutrients resulted in greater body weight (BW) gain in the C57BL/6ByJ mice than in the 129P3/J mice. The diet-induced weight gain was examined in detail in groups of 2-month-old C57BL/6ByJ and 129P3/J mice given ether chow, or chow and margarine to eat. Access to margarine did not increase total energy consumption of either strain. It increased BW and adiposity of the C57BL/6ByJ mice, but only after they reached the age of approximately 3 months. There were no differences in BW and adiposity between control and margarine-exposed 129P3/J mice. The results suggest that diet-induced adiposity in the B6 mice depends on age and does not depend on hyperphagia.
Assuntos
Dieta , Preferências Alimentares/fisiologia , Obesidade/psicologia , Animais , Composição Corporal/efeitos dos fármacos , Carboidratos da Dieta/farmacologia , Gorduras na Dieta/farmacologia , Substitutos da Gordura , Feminino , Glucanos/farmacologia , Masculino , Margarina , Camundongos , Camundongos Endogâmicos C57BLRESUMO
The Sac (saccharin preference) locus affecting mouse behavioral and neural responsiveness to sweeteners has been mapped to distal Chr 4. A putative sweet taste receptor, T1R1, has been recently cloned, and the gene encoding it, Gpr70, has also been mapped to mouse distal Chr 4. To assess Gpr70 as a candidate gene for Sac, we compared the Gpr70 sequences of C57BL/6ByJ and 129P3/J mouse strains with different alleles of Sac. Using Gpr70 sequence variation between the C57BL/6ByJ and 129P3/J strains, we conducted a high-resolution analysis of the chromosomal localization of the Gpr70 and Sac loci in the F2 hybrids and 129.B6-Sac partially congenic mice originating from these two strains. The Gpr70 gene maps proximal to Sac, which demonstrates that they are different loci.
Assuntos
Mapeamento Cromossômico , Receptores de Superfície Celular/genética , Receptores Acoplados a Proteínas G , Sacarina , Paladar/genética , Animais , Sequência de Bases , Nervo da Corda do Tímpano/fisiologia , Cruzamentos Genéticos , Feminino , Ligação Genética , Haplótipos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Dados de Sequência Molecular , Alinhamento de SequênciaRESUMO
The several linked polymorphic genes of the MHC, which has been proposed as a prime determinant of sensed genetic individuality within species, is known to operate in mice by olfactory recognition in aspects of reproductive behavior that concern mate selection, thereby favoring outbreeding and heterozygosity, and also concern the maintenance of pregnancy. A single base-change can alter an individual MHC odortype, and the potential range of combinatorial MHC-determined odortypes is clearly vast. Following our findings that newborn mice already express their MHC odortype (which is detectable at 9 days of gestational age), we sought to determine whether MHC is involved in behavioral aspects of early development, such as rearing. In the studies presented herein, we report the ability and proclivity of mothers to recognize and preferentially retrieve syngeneic (genetically identical) pups from other pups differing only for MHC. Reciprocally, we report the ability of pups to recognize their familial environment, regardless of whether they had been nursed by their biological mothers or by foster mothers. Early learning experiences of the MHC environment are apparently a key element in survival, assuring maternal protection and promoting outbreeding.
Assuntos
Comportamento Animal , Complexo Principal de Histocompatibilidade/genética , Odorantes , Animais , Animais Recém-Nascidos , Feminino , Camundongos , Camundongos Endogâmicos C57BL , GravidezRESUMO
Major histocompatibility complex (MHC) genes confer individual olfactory identity that can be detected with exquisite accuracy by mice. The fact that MHC genes themselves generate the characteristic odortype, rather than dedicated odor-determining genes, was supported in studies of point mutations in H2K and HLA transgenic mice, which evinced distinct odor profiles in olfactory assays. In this article we provide further evidence for a central role of MHC genes themselves in odortype specification by demonstrating that mice that are unable to express their genomic class I MHC genes because they lack beta2-microglobulin are distinguishable by scent from otherwise identical mice which possess an intact B2m gene. This odortype disparity appears at 9-12 days of gestational age, the period in which the MHC is first detectable in fetal cells of normal mice.
Assuntos
Complexo Principal de Histocompatibilidade , Odorantes , Microglobulina beta-2/fisiologia , Animais , Feminino , Generalização Psicológica , Aprendizagem em Labirinto , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Gravidez , Microglobulina beta-2/genéticaRESUMO
In two-bottle preference tests with water and solutions of monosodium glutamate (MSG) and inosine-5'-monophosphate (IMP), mice from the C57BL/6ByJ inbred strain consumed more and had higher preferences for these solutions compared with mice from the 129/J strain. The C57BL/6ByJ mice consumed 300 mmol/L MSG in large amounts, which were comparable to intakes of highly preferred solutions of sweeteners. The strain differences in voluntary consumption of 300 mmol/L MSG depended at least in part on postingestive effects because prior experience with MSG influenced the expression of the strain difference in MSG acceptance. The strain difference in MSG acceptance was in the opposite direction to the strain difference in NaCl acceptance and was not affected by previous consumption of saccharin. Although the C57BL/6ByJ mice had higher avidity for both MSG and sweeteners than did the 129/J mice, there was no correlation between preferences for these solutions in the second hybrid generation (F(2)) derived from these two strains. Thus, the strain differences in MSG acceptance are not related to the strain differences in salty or sweet taste responsiveness and most likely represent specific umami taste responsiveness. High acceptance of MSG solutions by the C57BL/6ByJ mice was inherited as a recessive trait in the F(2) generation. Further genetic linkage analyses using the F(2) hybrids are being conducted to map chromosomal locations of genes determining the strain difference in MSG acceptance.
Assuntos
Ingestão de Líquidos/efeitos dos fármacos , Aditivos Alimentares/farmacologia , Camundongos/fisiologia , Glutamato de Sódio/farmacologia , Paladar/efeitos dos fármacos , Animais , Feminino , Preferências Alimentares/fisiologia , Genes Recessivos/fisiologia , Hibridização Genética , Masculino , Camundongos/genética , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , Sacarina/farmacologia , Cloreto de Sódio/farmacologia , Soluções , Especificidade da Espécie , Edulcorantes/farmacologiaRESUMO
The present series of studies aimed to investigate whether experience with a flavor in mothers' milk modifies the infants' acceptance of similarly flavored foods at weaning. First, we established, using methods developed in our laboratory, that the ingestion of carrot juice by lactating women produced a sensory change in their milk approximately 2 to 3 hr after the ingestion of the beverage. Second, we randomly formed two groups of breast-fed infants who had been fed cereal for a few weeks but had only experienced cereal prepared with water. Their mothers were asked to consume one of two types of beverages (i.e., carrot juice, water) during the exposure period. Each mother was observed feeding her infant cereal during four test sessions. The first two sessions occurred during the 2 days before the exposure period; in counterbalanced order, infants were fed cereal prepared with water on 1 testing day and cereal prepared with carrot juice on the other. These two test sessions were then repeated following the exposure period. The results demonstrated that the infants who had exposure to the flavor of carrots in their mothers' milk during the exposure period consumed less of the carrot-flavored cereal and spent less time feeding when compared to the control infants whose mothers consumed the water. This may be a form of sensory-specific satiety such that the infants become less responsive to a flavor that they have been extensively exposed to in the very recent past.
Assuntos
Grão Comestível , Alimentos Infantis , Leite Humano , Psicologia da Criança , Paladar , Adulto , Bebidas , Daucus carota , Feminino , Humanos , Lactente , Masculino , Resposta de Saciedade , DesmameRESUMO
To investigate the possible role of peripheral gustatory responsiveness to changes in NaCl acceptance, we studied NaCl consumption and the chorda tympani nerve responses to lingual application of NaCl in C57BL/6ByJ mice. The mice were treated with 300 mM NaCl (given to drink in 96-h two-bottle tests with water) or with injections of deoxycorticosterone acetate (DOCA; 33 mg/kg daily). Naive mice were neutral to 75 mM NaCl, but mice previously exposed to 300 mM NaCl avoided 75 mM NaCl. The NaCl-exposed (300 mM for 4 days and 75 mM for 2 days) mice had enhanced amiloride-sensitive components of the chorda tympani responses to 10-30 mM NaCl applied at room temperature (24 degrees C). DOCA injections increased acceptance of 300 mM NaCl, but did not change the chorda tympani responses to 100-1000 mM NaCl. However, the DOCA-treated mice had enhanced amiloride-sensitive components of the chorda tympani responses to cold (12 degrees C) 10-30 mM NaCl. These data suggest that peripheral gustatory responsiveness possibly contributes to the NaCl aversion induced by exposure to concentrated NaCl, but not to the DOCA-induced increase of NaCl acceptance.
Assuntos
Comportamento Animal/efeitos dos fármacos , Desoxicorticosterona/farmacologia , Neurônios Aferentes/efeitos dos fármacos , Cloreto de Sódio/farmacologia , Paladar/efeitos dos fármacos , Animais , Nervo da Corda do Tímpano/fisiologia , Comportamento de Ingestão de Líquido/efeitos dos fármacos , Eletrofisiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , TemperaturaRESUMO
Odors that distinguish one individual from another member of the species and are determined by polymorphic genes are called odortypes. Odortypes and their considerable societal significance have been studied intimately only in mice and mainly with respect to the genes of the major histocompatibility complex. Further understanding and the matter of human relevance have been hampered by the apparent restriction of odortype expression to urine. The present finding that odorants comprising prerenal odortypes are already present in blood, albeit in masked form, affords the basis of a comprehensive view of odortypes. Accordingly, major histocompatibility complex and other polymorphic genes of antiquity are seen inter alia as agents of normal variation, which entails quantitative variation in output of odorant metabolites. Relatively few such normal variations should suffice for a vast range of compound odors whose specificity is determined by combinative assortment of the same set of individual volatile compounds.